Jane Zollinger - Academia.edu (original) (raw)

Papers by Jane Zollinger

Research paper thumbnail of Progress on the Development and Single-Laboratory Validation of a High-Performance Liquid Chromatographic Method for the Determination of Carbadox and Pyrantel Tartrate in Type B and C Medicated Feeds

Journal of AOAC INTERNATIONAL

Carbadox, an antimicrobial agent, and pyrantel tartrate, an anthelmintic, are feed additives that... more Carbadox, an antimicrobial agent, and pyrantel tartrate, an anthelmintic, are feed additives that are often used in combination in the United States. The current AOAC methods for these analytes are spectrophotometric, using standard addition techniques. These methods are labor-intensive and prone to variability as well as matrix interferences. Published methods for both analytes that use high-performance liquid chromatography were evaluated and a test method was developed. The method uses a water prewetting step to enhance extraction of pyrantel followed by extraction with acetonitrileethanol (50 + 50). Sample extracts are filtered through a glass fiber filter and purified using alumina solid-phase extraction columns. Chromatography is performed on a C18 column with a gradient mobile phase of dibutylamine acetate and acetonitrile. The data show that both analytes exhibit acceptable peak shape when a C18 column that is both acid- and base-deactivated is used. Linearity has been estab...

Research paper thumbnail of Determination of Nutritional and Cyclopropenoid Fatty Acids in Cottonseed by a Single GC Analysis

Journal of the American Oil Chemists' Society, 2015

Research paper thumbnail of Quantification of picomole amounts of leu-enkephalin by means of field desorption mass spectrometry

Biological Mass Spectrometry, 1981

Leu-enkephalin is quantified down to 30 ng (52 pmol) by means of field desorption mass spectromet... more Leu-enkephalin is quantified down to 30 ng (52 pmol) by means of field desorption mass spectrometry. Extensive chemical derivatization (acetylation, permethylation) is avoided. Linearity is obtained from 30 to 900 ng. INTRODUCTION The objective of this paper is to illustrate quantification of picomole (pmol) amounts of underivatized biologically important model oligopeptides by means of field desorption mass spectrometry (FDMS). This paper addresses quantification of Leu-enkephalin. Radioimmunoassay and bioassay data of biologic compounds must be confirmed by mass spectrometry and/or gaschromatography mass spectrometry (GCMS) analyses. Reports indicate that prostaglandins, steroids mela

Research paper thumbnail of High-performance liquid chromatography and field desorption mass spectrometry of hypothalamic oligopeptides

Journal of Chromatography A, 1980

A high-performance liquid chromatographic system employing a chemically bonded alhyl phase and a ... more A high-performance liquid chromatographic system employing a chemically bonded alhyl phase and a tetraalkylammonium phosphate bufber has been evaluated for separation of synthetic mixtures of hypothalamic oligopeptides. Separation of two peptides in the mixture having very simiiar adjusted retention times has been achieved. Sensitivity down to 5 ng is attained. Peptides range in sire from three to 31 amino acids. Field desorption mass spectra illustrate novel structural elucidation methods for individual peptides and signal a quantitkation method. I~ODUCIION This paper describes a high-petiormance liquid chromatogmphic (HPLC) method to separate mixtures of biological oligopeptides in a fast and facile manner, optimizing sensitivity, speed and resolution of the separation to provide a purified peptide fraction for quantification and structural elucidation studies. Towatds this end, the triethylamine phosphate (TEAP)-acetonitrile system is used with a @3ondapak C,, HPLC column. Synthetic mixtmes of hypothalamic oligopeptides coutaining from three to 31 amino acids are employed_ A field desorption (FD) spectmm of an underivatized hcxapeptide illustrates the utility of the novel ionization method in peptide chemistry to provide molecular ions of underivatized oligopeptides and for quantifkation. Reversed-phase (RP) HPLC using chemically bonded al&l stationary phases has become an important and powerful tool in peptide separation chemistry. Long chain al@1 silanes are chemically bonded to silanol groups located on the silicon surface of the packing material. An end-capping reaction follows where trimethylsiiyl groups are chemically bonded to most of the re maining unreacted silanol groups. Various authors reviewed HPLC column technology and provided a theoretical basis for separations afforded by RP col~unns~-~. HPLC retention indices related to compound lipophilicity were estimated'. Ho&&h and co-worker&' show separation on RP columns is a fbn&on of increasin g hydrophobic&y of the peptide-paired-ionsolvent hydration complex.. ml-9673p~ @0225

Research paper thumbnail of Field desorption collision activation linked scanning mass spectrometry of underivatized oligopeptides

Biological Mass Spectrometry, 1981

Field desorption production of protonated molecular ions from nanomole amounts of chemically unde... more Field desorption production of protonated molecular ions from nanomole amounts of chemically underivatized penta-, tetra-and dipeptides, coupled with collision induced decomposition of [M + HI' ions, yielded partial amino acid sequence information. Linked scanning of two of the three fields detected ions produced in the first field free region of a double focusing mass spectrometer.

Research paper thumbnail of Field desorption collision activation linked scanning mass spectrometry of underivatized oligopeptides

Biological Mass Spectrometry, 1981

Field desorption production of protonated molecular ions from nanomole amounts of chemically unde... more Field desorption production of protonated molecular ions from nanomole amounts of chemically underivatized penta-, tetra-and dipeptides, coupled with collision induced decomposition of [M + HI' ions, yielded partial amino acid sequence information. Linked scanning of two of the three fields detected ions produced in the first field free region of a double focusing mass spectrometer.

Research paper thumbnail of In Vitro Effects of Fatty Acids on the Actions of Serum on Rat and Pig Cartilage

Hormone and Metabolic Research, 1979

It has been reported that fatty acids preferentially inhibit serum-stimulated incorporation of su... more It has been reported that fatty acids preferentially inhibit serum-stimulated incorporation of sulfate by embryonic chick cartilage, suggesting that they may interfere with the effects of a proposed mediator (serum somatomedin) of the actions of growth hormone (GH). This was studied further in mammalian cartilage. Butyrate and octanoate at concentrations of 0.5 to 5 mM produced a concentration-dependent inhibition of both basal and serum-stimulated sulfate and thymidine incorporation by costal cartilage from hypophysectomized rats. Butyrate also inhibited basal and serum-stimulated sulfate incorporation in cartilage from normal pigs and normal sucklings rats. In all 3 test systems, oleate (0.2--5 mM) bound to serum albumin (4 g/dl) was ineffective. There was no evidence that fatty acids preferentially inhibited the stimulation of sulfate incorporation produced by serum.

Research paper thumbnail of Progress on the Development and Single-Laboratory Validation of a High-Performance Liquid Chromatographic Method for the Determination of Carbadox and Pyrantel Tartrate in Type B and C Medicated Feeds

Journal of AOAC INTERNATIONAL

Carbadox, an antimicrobial agent, and pyrantel tartrate, an anthelmintic, are feed additives that... more Carbadox, an antimicrobial agent, and pyrantel tartrate, an anthelmintic, are feed additives that are often used in combination in the United States. The current AOAC methods for these analytes are spectrophotometric, using standard addition techniques. These methods are labor-intensive and prone to variability as well as matrix interferences. Published methods for both analytes that use high-performance liquid chromatography were evaluated and a test method was developed. The method uses a water prewetting step to enhance extraction of pyrantel followed by extraction with acetonitrileethanol (50 + 50). Sample extracts are filtered through a glass fiber filter and purified using alumina solid-phase extraction columns. Chromatography is performed on a C18 column with a gradient mobile phase of dibutylamine acetate and acetonitrile. The data show that both analytes exhibit acceptable peak shape when a C18 column that is both acid- and base-deactivated is used. Linearity has been estab...

Research paper thumbnail of Determination of Nutritional and Cyclopropenoid Fatty Acids in Cottonseed by a Single GC Analysis

Journal of the American Oil Chemists' Society, 2015

Research paper thumbnail of Quantification of picomole amounts of leu-enkephalin by means of field desorption mass spectrometry

Biological Mass Spectrometry, 1981

Leu-enkephalin is quantified down to 30 ng (52 pmol) by means of field desorption mass spectromet... more Leu-enkephalin is quantified down to 30 ng (52 pmol) by means of field desorption mass spectrometry. Extensive chemical derivatization (acetylation, permethylation) is avoided. Linearity is obtained from 30 to 900 ng. INTRODUCTION The objective of this paper is to illustrate quantification of picomole (pmol) amounts of underivatized biologically important model oligopeptides by means of field desorption mass spectrometry (FDMS). This paper addresses quantification of Leu-enkephalin. Radioimmunoassay and bioassay data of biologic compounds must be confirmed by mass spectrometry and/or gaschromatography mass spectrometry (GCMS) analyses. Reports indicate that prostaglandins, steroids mela

Research paper thumbnail of High-performance liquid chromatography and field desorption mass spectrometry of hypothalamic oligopeptides

Journal of Chromatography A, 1980

A high-performance liquid chromatographic system employing a chemically bonded alhyl phase and a ... more A high-performance liquid chromatographic system employing a chemically bonded alhyl phase and a tetraalkylammonium phosphate bufber has been evaluated for separation of synthetic mixtures of hypothalamic oligopeptides. Separation of two peptides in the mixture having very simiiar adjusted retention times has been achieved. Sensitivity down to 5 ng is attained. Peptides range in sire from three to 31 amino acids. Field desorption mass spectra illustrate novel structural elucidation methods for individual peptides and signal a quantitkation method. I~ODUCIION This paper describes a high-petiormance liquid chromatogmphic (HPLC) method to separate mixtures of biological oligopeptides in a fast and facile manner, optimizing sensitivity, speed and resolution of the separation to provide a purified peptide fraction for quantification and structural elucidation studies. Towatds this end, the triethylamine phosphate (TEAP)-acetonitrile system is used with a @3ondapak C,, HPLC column. Synthetic mixtmes of hypothalamic oligopeptides coutaining from three to 31 amino acids are employed_ A field desorption (FD) spectmm of an underivatized hcxapeptide illustrates the utility of the novel ionization method in peptide chemistry to provide molecular ions of underivatized oligopeptides and for quantifkation. Reversed-phase (RP) HPLC using chemically bonded al&l stationary phases has become an important and powerful tool in peptide separation chemistry. Long chain al@1 silanes are chemically bonded to silanol groups located on the silicon surface of the packing material. An end-capping reaction follows where trimethylsiiyl groups are chemically bonded to most of the re maining unreacted silanol groups. Various authors reviewed HPLC column technology and provided a theoretical basis for separations afforded by RP col~unns~-~. HPLC retention indices related to compound lipophilicity were estimated'. Ho&&h and co-worker&' show separation on RP columns is a fbn&on of increasin g hydrophobic&y of the peptide-paired-ionsolvent hydration complex.. ml-9673p~ @0225

Research paper thumbnail of Field desorption collision activation linked scanning mass spectrometry of underivatized oligopeptides

Biological Mass Spectrometry, 1981

Field desorption production of protonated molecular ions from nanomole amounts of chemically unde... more Field desorption production of protonated molecular ions from nanomole amounts of chemically underivatized penta-, tetra-and dipeptides, coupled with collision induced decomposition of [M + HI' ions, yielded partial amino acid sequence information. Linked scanning of two of the three fields detected ions produced in the first field free region of a double focusing mass spectrometer.

Research paper thumbnail of Field desorption collision activation linked scanning mass spectrometry of underivatized oligopeptides

Biological Mass Spectrometry, 1981

Field desorption production of protonated molecular ions from nanomole amounts of chemically unde... more Field desorption production of protonated molecular ions from nanomole amounts of chemically underivatized penta-, tetra-and dipeptides, coupled with collision induced decomposition of [M + HI' ions, yielded partial amino acid sequence information. Linked scanning of two of the three fields detected ions produced in the first field free region of a double focusing mass spectrometer.

Research paper thumbnail of In Vitro Effects of Fatty Acids on the Actions of Serum on Rat and Pig Cartilage

Hormone and Metabolic Research, 1979

It has been reported that fatty acids preferentially inhibit serum-stimulated incorporation of su... more It has been reported that fatty acids preferentially inhibit serum-stimulated incorporation of sulfate by embryonic chick cartilage, suggesting that they may interfere with the effects of a proposed mediator (serum somatomedin) of the actions of growth hormone (GH). This was studied further in mammalian cartilage. Butyrate and octanoate at concentrations of 0.5 to 5 mM produced a concentration-dependent inhibition of both basal and serum-stimulated sulfate and thymidine incorporation by costal cartilage from hypophysectomized rats. Butyrate also inhibited basal and serum-stimulated sulfate incorporation in cartilage from normal pigs and normal sucklings rats. In all 3 test systems, oleate (0.2--5 mM) bound to serum albumin (4 g/dl) was ineffective. There was no evidence that fatty acids preferentially inhibited the stimulation of sulfate incorporation produced by serum.