Janice Marcon - Academia.edu (original) (raw)

Papers by Janice Marcon

Research paper thumbnail of Posttranslational modification at the N terminus of the human adenovirus type 12 E1A 235R tumor antigen

Journal of Virology, 1986

The adenovirus E1A transforming region, which encodes immortalization, partial cell transformatio... more The adenovirus E1A transforming region, which encodes immortalization, partial cell transformation, and gene activation functions, expresses two early mRNAs, 13S and 12S. Multiple-T antigen species with different electrophoretic mobilities are formed from each mRNA, presumably by unknown posttranslational modifications. The adenovirus type 12 (Ad12) 13S and 12S mRNAs encode E1A T antigens of 266 and 235 amino acid residues (266R and 235R), respectively. To study possible posttranslational processing at the N and C termini and to distinguish between the Ad12 266R and 235R T antigens, we prepared antibodies targeted to synthetic peptides encoded at the common C (peptide 204) and N (peptide 202) termini of the 266R and 235R T antigens and at the unique internal domain of the 266R T antigen (peptide 206). The specificity of each anti-peptide antibody was confirmed by immunoprecipitation of the 266R and 235R T antigens produced in Escherichia coli. Immunoprecipitation analysis of the E1A...

Research paper thumbnail of Biosynthesis of adenovirus type 2 i-leader protein

Journal of Virology, 1986

The i-leader is a 440-base-pair sequence located between 21.8 and 23.0 map units on the adenoviru... more The i-leader is a 440-base-pair sequence located between 21.8 and 23.0 map units on the adenovirus type 2 genome and is spliced between the second and third segments of the major tripartite leader in certain viral mRNA molecules. The i-leader contains an open translational reading frame for a hypothetical protein of Mr about 16,600, and a 16,000-Mr polypeptide (16K protein) has been translated in vitro on mRNA selected with DNA containing the i-leader (A. Virtanen, P. Aleström, H. Persson, M. G. Katze, and U. Pettersson, Nucleic Acids Res. 10:2539-2548, 1982). To determine whether the i-leader protein is synthesized during productive infection and to provide an immunological reagent to study the properties and functions of the i-leader protein, we prepared antipeptide antibodies directed to a 16-amino acid synthetic peptide which is encoded near the N terminus of the hypothetical i-leader protein and contains a high acidic amino acid and proline content. Antipeptide antibodies immun...

Research paper thumbnail of The 55K protein on the 5' termini of adenovirus type 2 DNA is unrelated to virus-coded candidate transformation proteins (E1-53K, E1-40K-50K) and DNA-binding proteins (E2-42K/47K/73K)

Journal of Virology, 1979

A polypeptide of 55,000 daltons (55K) is linked, probably covalently, to the K' termini of ad... more A polypeptide of 55,000 daltons (55K) is linked, probably covalently, to the K' termini of adenovirus type 2 DNA. The 55K polypeptide is synthesized during early stages of infection (T. Yamashita, M. Arens, and M. Green, J. Virol. 30: 497-507, 1979) and thus may function in viral DNA replication, gene regulation, or cell transformation. Several virus-coded early polypeptides have been identified that could correspond to the terminal 55K, including the E1-40K-50K and E1-53K candidate transformation polypeptides and the E2-42K/47K/73K single-stranded DNA-binding polypeptide. We show here that two-dimensional tryptic [35S]methionine-peptide maps of the terminal 55K differ completely from [35S]methionine-peptide maps of four related E1-40K-50K polypeptides, the E1-53K, and the related E2-42K, E2-47K, and E2-73K polypeptides. We conclude that the terminal 55K polypeptide does not correspond to any of the known virus-coded early polypeptides.

Research paper thumbnail of Genome expression and mRNA maturation at late stages of productive adenovirus type 2 infection

Journal of Virology, 1976

RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral h... more RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand from which they originated. Early whole cell RNA extracted 5 h postinfection annealed rapidly to 10 to 15% of l and r strands and then slowly to final values of 60 and 40% of l and r strands. By 9 h postinfection the expression of late genes was apparent and whole cell RNA annealed to 20 and 75% of l and r strands. Whole cell RNA extracted between 12 and 36 h postinfection annealed to 7 to 15% and 75 to 90% of l and r strands. Late nuclear RNA hybridized to 10 and 90% of l and r strands, and late polyribosomal RNA hybridized to 20 and 75% of l and r strands. Based upon kinetic analyses, we estimate that mRNA synthesized exclusively during late stages arises from about 6 to...

Research paper thumbnail of Large-Scale Purification of Ribonucleic Acid Tumor Viruses by Use of Continuous-Flow Density Gradient Centrifugation

Applied Microbiology, 1973

This report describes a 200to 300-fold increase in the quantity of ribonucleic acid tumor virus p... more This report describes a 200to 300-fold increase in the quantity of ribonucleic acid tumor virus particles previously isolated at one time by zonal centrifugation.

Research paper thumbnail of Identification ofAdenovirus 12Encoded ElATumorAntigens Synthesized inInfected andTransformed MammalianCells andin Escherichia coli

Research paper thumbnail of Studies on Early Proteins and Transformation Proteins of Human Adenoviruses

Cold Spring Harbor Symposia on Quantitative Biology, 1980

Research paper thumbnail of Adenovirus-2 DNA: In Vitro Replication and Analysis of Viral Early Proteins

Cold Spring Harbor Symposia on Quantitative Biology, 1979

Research paper thumbnail of Brincando e Aprendendo com o Jogo: A Trilha das Operações

O objetivo principal deste artigo é apresentar e relatar uma experiência realizada no Atendimento... more O objetivo principal deste artigo é apresentar e relatar uma experiência realizada no Atendimento Educacional Especializado do polo Alberto Bauer, na cidade de Jaraguá do Sul-SC, no ano de 2014. Compreende-se que é um desafio ensinar matemática para alunos com deficiência e, por isso, sempre são buscadas estratégias metodológicas diferenciadas. Nesse sentido, a utilização de jogos tem sido sempre uma boa opção, pois motiva os alunos para a aprendizagem de uma forma lúdica. Nesta experiência, será mostrado como foi criado e aplicado com os alunos o jogo "Trilha das Operações". Levando em consideração que o processo de aprendizagem é longo e que cada aluno aprende no seu tempo, este projeto foi executado durante o ano todo, avançando o nível de dificuldade conforme o progresso de cada aluno. A avaliação foi feita por meio de observação sobre a autonomia que cada aluno demonstrou ao realizar as atividades. Palavras-chave: Apr endizagem matemática. Alunos com deficiência. J ogos. E d u c a ç ã o M a t e má t i c a e m R e v i s t a SOCIEDADE BRASILEIRA DE EDUCAÇÃO MATEMÁTICA

Research paper thumbnail of Posttranslational modification at the N terminus of the human adenovirus type 12 E1A 235R tumor antigen

Journal of Virology, 1986

The adenovirus E1A transforming region, which encodes immortalization, partial cell transformatio... more The adenovirus E1A transforming region, which encodes immortalization, partial cell transformation, and gene activation functions, expresses two early mRNAs, 13S and 12S. Multiple-T antigen species with different electrophoretic mobilities are formed from each mRNA, presumably by unknown posttranslational modifications. The adenovirus type 12 (Ad12) 13S and 12S mRNAs encode E1A T antigens of 266 and 235 amino acid residues (266R and 235R), respectively. To study possible posttranslational processing at the N and C termini and to distinguish between the Ad12 266R and 235R T antigens, we prepared antibodies targeted to synthetic peptides encoded at the common C (peptide 204) and N (peptide 202) termini of the 266R and 235R T antigens and at the unique internal domain of the 266R T antigen (peptide 206). The specificity of each anti-peptide antibody was confirmed by immunoprecipitation of the 266R and 235R T antigens produced in Escherichia coli. Immunoprecipitation analysis of the E1A...

Research paper thumbnail of Biosynthesis of adenovirus type 2 i-leader protein

Journal of Virology, 1986

The i-leader is a 440-base-pair sequence located between 21.8 and 23.0 map units on the adenoviru... more The i-leader is a 440-base-pair sequence located between 21.8 and 23.0 map units on the adenovirus type 2 genome and is spliced between the second and third segments of the major tripartite leader in certain viral mRNA molecules. The i-leader contains an open translational reading frame for a hypothetical protein of Mr about 16,600, and a 16,000-Mr polypeptide (16K protein) has been translated in vitro on mRNA selected with DNA containing the i-leader (A. Virtanen, P. Aleström, H. Persson, M. G. Katze, and U. Pettersson, Nucleic Acids Res. 10:2539-2548, 1982). To determine whether the i-leader protein is synthesized during productive infection and to provide an immunological reagent to study the properties and functions of the i-leader protein, we prepared antipeptide antibodies directed to a 16-amino acid synthetic peptide which is encoded near the N terminus of the hypothetical i-leader protein and contains a high acidic amino acid and proline content. Antipeptide antibodies immun...

Research paper thumbnail of The 55K protein on the 5' termini of adenovirus type 2 DNA is unrelated to virus-coded candidate transformation proteins (E1-53K, E1-40K-50K) and DNA-binding proteins (E2-42K/47K/73K)

Journal of Virology, 1979

A polypeptide of 55,000 daltons (55K) is linked, probably covalently, to the K' termini of ad... more A polypeptide of 55,000 daltons (55K) is linked, probably covalently, to the K' termini of adenovirus type 2 DNA. The 55K polypeptide is synthesized during early stages of infection (T. Yamashita, M. Arens, and M. Green, J. Virol. 30: 497-507, 1979) and thus may function in viral DNA replication, gene regulation, or cell transformation. Several virus-coded early polypeptides have been identified that could correspond to the terminal 55K, including the E1-40K-50K and E1-53K candidate transformation polypeptides and the E2-42K/47K/73K single-stranded DNA-binding polypeptide. We show here that two-dimensional tryptic [35S]methionine-peptide maps of the terminal 55K differ completely from [35S]methionine-peptide maps of four related E1-40K-50K polypeptides, the E1-53K, and the related E2-42K, E2-47K, and E2-73K polypeptides. We conclude that the terminal 55K polypeptide does not correspond to any of the known virus-coded early polypeptides.

Research paper thumbnail of Genome expression and mRNA maturation at late stages of productive adenovirus type 2 infection

Journal of Virology, 1976

RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral h... more RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand from which they originated. Early whole cell RNA extracted 5 h postinfection annealed rapidly to 10 to 15% of l and r strands and then slowly to final values of 60 and 40% of l and r strands. By 9 h postinfection the expression of late genes was apparent and whole cell RNA annealed to 20 and 75% of l and r strands. Whole cell RNA extracted between 12 and 36 h postinfection annealed to 7 to 15% and 75 to 90% of l and r strands. Late nuclear RNA hybridized to 10 and 90% of l and r strands, and late polyribosomal RNA hybridized to 20 and 75% of l and r strands. Based upon kinetic analyses, we estimate that mRNA synthesized exclusively during late stages arises from about 6 to...

Research paper thumbnail of Large-Scale Purification of Ribonucleic Acid Tumor Viruses by Use of Continuous-Flow Density Gradient Centrifugation

Applied Microbiology, 1973

This report describes a 200to 300-fold increase in the quantity of ribonucleic acid tumor virus p... more This report describes a 200to 300-fold increase in the quantity of ribonucleic acid tumor virus particles previously isolated at one time by zonal centrifugation.

Research paper thumbnail of Identification ofAdenovirus 12Encoded ElATumorAntigens Synthesized inInfected andTransformed MammalianCells andin Escherichia coli

Research paper thumbnail of Studies on Early Proteins and Transformation Proteins of Human Adenoviruses

Cold Spring Harbor Symposia on Quantitative Biology, 1980

Research paper thumbnail of Adenovirus-2 DNA: In Vitro Replication and Analysis of Viral Early Proteins

Cold Spring Harbor Symposia on Quantitative Biology, 1979

Research paper thumbnail of Brincando e Aprendendo com o Jogo: A Trilha das Operações

O objetivo principal deste artigo é apresentar e relatar uma experiência realizada no Atendimento... more O objetivo principal deste artigo é apresentar e relatar uma experiência realizada no Atendimento Educacional Especializado do polo Alberto Bauer, na cidade de Jaraguá do Sul-SC, no ano de 2014. Compreende-se que é um desafio ensinar matemática para alunos com deficiência e, por isso, sempre são buscadas estratégias metodológicas diferenciadas. Nesse sentido, a utilização de jogos tem sido sempre uma boa opção, pois motiva os alunos para a aprendizagem de uma forma lúdica. Nesta experiência, será mostrado como foi criado e aplicado com os alunos o jogo "Trilha das Operações". Levando em consideração que o processo de aprendizagem é longo e que cada aluno aprende no seu tempo, este projeto foi executado durante o ano todo, avançando o nível de dificuldade conforme o progresso de cada aluno. A avaliação foi feita por meio de observação sobre a autonomia que cada aluno demonstrou ao realizar as atividades. Palavras-chave: Apr endizagem matemática. Alunos com deficiência. J ogos. E d u c a ç ã o M a t e má t i c a e m R e v i s t a SOCIEDADE BRASILEIRA DE EDUCAÇÃO MATEMÁTICA