Jaroslav Kobliha - Academia.edu (original) (raw)
Papers by Jaroslav Kobliha
Tree Genetics & Genomes, 2011
We developed a deterministic model to optimize DNA fingerprinting effort in the presence of gene ... more We developed a deterministic model to optimize DNA fingerprinting effort in the presence of gene flow during the application of Breeding without Breeding. The method considers trait's heritability, level of gene flow, selection differential, and the proportion of progeny test subjected to fingerprinting (truncation). All the model's variables individually or in concert proved important; however, the trait's heritability magnitude played the most important role in minimizing the selection of contaminated individuals and it could be used as a surrogate to traits with low heritability.
Tree Genetics & Genomes, 2012
Tree Genetics & Genomes, 2013
Nine microsatellite DNA markers (simple sequence repeats, SSRs) were used to estimate pairwise re... more Nine microsatellite DNA markers (simple sequence repeats, SSRs) were used to estimate pairwise relationships among 597 Scots pine (Pinus sylvestris) trees as well as to generate a sibship structure for quantitative genetic parameters' estimation comparison. The studied trees were part of an open-pollinated progeny test of 102 first-generation parents. Three methods were used to estimate variance components and heritabilities, namely, structured pedigree (half-and fullsib), marker-based pairwise relationships (four pairwise estimators), and a combined pedigree and marker-based relationship. In each of the three methods, the same animal model was used to compute variances except when marker-based relationship was used wherein we substituted the average numerator relationship matrix (i.e., pedigree-based matrix) with that computed based on markers' pairwise relationships. Our results showed a high correlation in estimated breeding values between the pedigree (full-sib) and the combined marker-pedigree estimates. The marker-based relationship method produced high correlations when individual site data were analyzed. In contrast, the marker-based relationship method resulted in a significant decrease in both variance estimation and their standard errors which were in concordance with earlier published results; however, no estimates were produced when across-site analyses were attempted. We concluded that the combined pedigree method is the best approach as it represents the historical (pairwise) and contemporary (pedigree) relationships among the tested individuals, a situation that cannot be attained by any of the used methods individually. This method is dependent on the number and informativeness of the markers used.
Plant Systematics and Evolution, 2013
Crossability relationships between six species
J. FOR. SCI, 2007
Two exemplary black spruce (Picea mariana [Mill.] B.S.P.) provenance trials were analyzed using t... more Two exemplary black spruce (Picea mariana [Mill.] B.S.P.) provenance trials were analyzed using traditional and spatial techniques. The objective was to find out possible differences between these approaches in terms of both the resulting fit-statistics and the estimated mean heights of provenances. Further, the spatial model was consequently adjusted to treat global and extraneous sources of variation. As expected, models incorporating spatial variation provided a better fit to the data. Consequently, there was also a noticeable shift in ranking of individual provenances, which has an important implication for the interpretation of provenance experiments results. Problems associated with the analysis of traditional randomized block designs in forestry research are discussed.
Acta Biologica Cracoviensia Series Botanica, 2000
The present paper deals with data obtained from fifteen years old Norway spruce ( Picea abies (L.... more The present paper deals with data obtained from fifteen years old Norway spruce ( Picea abies (L.) Karst.) progeny test established at three sites in the Sázava River region. Parameter under the evaluation was a tree height in 15 years following the establishment of the trial. Genetic parameters were estimated using the REML (Restricted Maxi - mum Likelihood) procedure followed
Tree Genetics & Genomes, 2011
We developed a deterministic model to optimize DNA fingerprinting effort in the presence of gene ... more We developed a deterministic model to optimize DNA fingerprinting effort in the presence of gene flow during the application of Breeding without Breeding. The method considers trait's heritability, level of gene flow, selection differential, and the proportion of progeny test subjected to fingerprinting (truncation). All the model's variables individually or in concert proved important; however, the trait's heritability magnitude played the most important role in minimizing the selection of contaminated individuals and it could be used as a surrogate to traits with low heritability.
Tree Genetics & Genomes, 2012
Tree Genetics & Genomes, 2013
Nine microsatellite DNA markers (simple sequence repeats, SSRs) were used to estimate pairwise re... more Nine microsatellite DNA markers (simple sequence repeats, SSRs) were used to estimate pairwise relationships among 597 Scots pine (Pinus sylvestris) trees as well as to generate a sibship structure for quantitative genetic parameters' estimation comparison. The studied trees were part of an open-pollinated progeny test of 102 first-generation parents. Three methods were used to estimate variance components and heritabilities, namely, structured pedigree (half-and fullsib), marker-based pairwise relationships (four pairwise estimators), and a combined pedigree and marker-based relationship. In each of the three methods, the same animal model was used to compute variances except when marker-based relationship was used wherein we substituted the average numerator relationship matrix (i.e., pedigree-based matrix) with that computed based on markers' pairwise relationships. Our results showed a high correlation in estimated breeding values between the pedigree (full-sib) and the combined marker-pedigree estimates. The marker-based relationship method produced high correlations when individual site data were analyzed. In contrast, the marker-based relationship method resulted in a significant decrease in both variance estimation and their standard errors which were in concordance with earlier published results; however, no estimates were produced when across-site analyses were attempted. We concluded that the combined pedigree method is the best approach as it represents the historical (pairwise) and contemporary (pedigree) relationships among the tested individuals, a situation that cannot be attained by any of the used methods individually. This method is dependent on the number and informativeness of the markers used.
Plant Systematics and Evolution, 2013
Crossability relationships between six species
J. FOR. SCI, 2007
Two exemplary black spruce (Picea mariana [Mill.] B.S.P.) provenance trials were analyzed using t... more Two exemplary black spruce (Picea mariana [Mill.] B.S.P.) provenance trials were analyzed using traditional and spatial techniques. The objective was to find out possible differences between these approaches in terms of both the resulting fit-statistics and the estimated mean heights of provenances. Further, the spatial model was consequently adjusted to treat global and extraneous sources of variation. As expected, models incorporating spatial variation provided a better fit to the data. Consequently, there was also a noticeable shift in ranking of individual provenances, which has an important implication for the interpretation of provenance experiments results. Problems associated with the analysis of traditional randomized block designs in forestry research are discussed.
Acta Biologica Cracoviensia Series Botanica, 2000
The present paper deals with data obtained from fifteen years old Norway spruce ( Picea abies (L.... more The present paper deals with data obtained from fifteen years old Norway spruce ( Picea abies (L.) Karst.) progeny test established at three sites in the Sázava River region. Parameter under the evaluation was a tree height in 15 years following the establishment of the trial. Genetic parameters were estimated using the REML (Restricted Maxi - mum Likelihood) procedure followed