Jaroslav Salava - Academia.edu (original) (raw)

Papers by Jaroslav Salava

Research paper thumbnail of Origin and diversity of the sources of resistance to PPV in apricot: implications and use in marker-assisted resistance to sharka

HAL (Le Centre pour la Communication Scientifique Directe), Sep 3, 2013

HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Research paper thumbnail of A genetic linkage map for an apricot (Prunus armeniaca L.) BC1 population mapping plum pox virus resistance

Tree Genetics & Genomes, 2008

Plum pox virus (sharka; PPV) can cause severe crop loss in economically important Prunus species ... more Plum pox virus (sharka; PPV) can cause severe crop loss in economically important Prunus species such as peach, plum, apricot, and cherry. Of these species, certain apricot cultivars ('Stark Early Orange', 'Goldrich', 'Harlayne') display significant levels of resistance to the disease and are the genetic substrate for studies of several xlaboratories working cooperatively to genetically characterize and mark the resistance locus or loci for marker-assisted breeding. The goals of the work presented in this communication are the characterization of the genetics of PPV resistance in 'Stark Early Orange' and the development of co-dominant molecular markers for marker-assisted selection (MAS) in PPV resistance breeding. We present the first genetic linkage map for an apricot backcross population of 'Stark Early Orange' and the susceptible cultivar 'Vestar' that segregates for resistance to PPV. This map is comprised of 357 loci (330 amplified fragment length polymorphisms (AFLPs), 26 simple sequence repeats (SSRs), and 1 morphological marker for PPV resistance) assigned to eight linkage groups. Twentytwo of the mapped SSRs are shared in common with genetic reference map for Prunus (T × E; Joobeur et al. 1998) and anchor our apricot map to the general Prunus map. A PPV resistance locus was mapped in linkage group 1 and four AFLP markers segregating with the PPV resistance trait, identified through bulk segregant analysis, facilitated the development of SSRs in this region.

Research paper thumbnail of Construction of a Genetic Map for Apricot with Molecular Markers and Identification of Markers Associated with Plum Pox Virus Resistance

International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species, 2007

Plum pox virus (PPV) causes serious damage in apricots and breeding and growing of PPV-resistant ... more Plum pox virus (PPV) causes serious damage in apricots and breeding and growing of PPV-resistant apricot cultivars are the only way of its efficient control. An integrated genetic linkage map for apricot (Prunus armeniaca L.) was obtained using a BC 1 progeny 'LE-3246' x 'Vestar'. A total of 316 molecular markers (290 AFLPs, 26 SSRs) and the resistance to PPV were assigned to 8 linkage groups covering 574 cM of the apricot genome. The average distance between adjacent loci is 2.5 cM. A PPVres1 locus conditioning resistance to PPV was mapped in linkage group 1. Several AFLPs and an SSR tightly linked to PPVres1 locus were identified. The construction of a genetic map for apricot and identification of molecular markers linked to PPV resistance will facilitate the breeder to obtain resistant apricot cultivars.

Research paper thumbnail of Genetic Diversity between Yacon Landraces from Different Countries Based on Random Amplified Polymorphic DNAs

Czech Journal of Genetics and Plant Breeding, 2011

Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relati... more Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relationship of crop germplasm. Five different landraces of yacon (Smallantus sonchifolius (Poepp. and Hendl.) H. Robinson; Asteraceae) collected in various countries and showing different morphological traits were investigated using a total of 61 decamer primers. A total of 282 RAPD markers were scored and 28.7% of them were polymorphic at least within landraces. RAPD markers generated by one primer (OBP14) discriminated between all landraces. Markers were used to calculate genetic similarity coefficient and to build a dendrogram representing the genetic relationship between analysed landraces. The results suggest that RAPD markers could be used as a reliable tool to perform fingerprinting studies in Smallantus sonchifolius genome. This is the first report on the use of RAPDs to evaluate genetic distance and to distinguish between different landraces in yacon.

Research paper thumbnail of Application of molecular markers in breeding for resistance to the Plum Pox Virus in Prunus spp

HAL (Le Centre pour la Communication Scientifique Directe), Jan 13, 2007

Research paper thumbnail of The emergence of a atrazine resistant black nightshade (Solanum nigrum L.) biotype and molecular basis of the resistance

Plant Protection Science, 2010

Seeds from atrazine resistant plants of black nightshade (<I>Solanum nigrum </I>L.)&l... more Seeds from atrazine resistant plants of black nightshade (<I>Solanum nigrum </I>L.)<I> </I>were collected at the railway station Prague-Vršovice, seeds from susceptible plants in Vyšehořovice (Prague East district). Tests on emergence showed that in both resistant and susceptible biotypes it was highest at a seeding depth of 1 mm, and that at the same seeding depth there were statistically significant differences in emergence between the resistant and susceptible biotypes. The resistance or susceptibility to atrazine was tested by both a chlorophyll fluorescence assay and spraying with atrazine. A region of the gene encoding D1 protein of photosystem II was sequenced and compared between the resistant and susceptible biotypes. Resistance to atrazine in the <I>S. nigrum</I> biotype from Vršovice was conferred by a glycine for serine substitution at residue 264 of the D1 protein. In the plants of the biotypes there was excellent correspondence betwe...

Research paper thumbnail of Changes in baking quality of winter wheat with different intensity of Fusarium spp. contamination detected by means of new rheological system

Czech Journal of Food Sciences, 2011

The aim of our work was to assess the possibility of detecting the changes in the baking quality ... more The aim of our work was to assess the possibility of detecting the changes in the baking quality of winter wheat with different levels of Fusarium spp. contamination using a new rheological system Mixolab, and to determine the correlations between the Mixolab characteristics and other quality parameters of wheat flour and grain. The standard technological characteristics (crude protein, Zeleny sedimentation index, wet gluten, falling number), loaf volume, shape features of bread (height and diameter), Mixolab parameters, and mycotoxin deoxynivalenol (DON) content were determined in 3 winter wheat cultivars (Akteur – quality group E – elite; Eurofit – quality group A; Meritto – quality group B) with different levels of Fusarium spp. contamination (8 variants) in two years. Increasing intensity of Fusarium spp. contamination evidently worsened the rheological quality and its negative effects on protein and mainly on the starch part of the grain was obvious in Mixolab curves. High corr...

Research paper thumbnail of Methodology for the phenotyping resistance to Plum pox virus in peach

Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important v... more Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important virus disease in fruit trees worldwide. Since it is not possible to completely eradicate PPV in the Czech Republic, it is necessary to find another way to control this pathogen. The most promising seems to be the breeding of new cultivars for resistance. This methodology provides a comprehensive protocol for testing PPV resistance in peach. It is based on visual assessment of disease symptoms on leaves and fruits, and determining the presence of the virus in plants using ELISA and RT-PCR techniques

Research paper thumbnail of Mendelian inheritance of introrse orientated anthers in Brassica rapa

Czech Journal of Genetics and Plant Breeding, 2022

The inheritance of anther orientation of 154 individuals from two B1 populations of Brassica rapa... more The inheritance of anther orientation of 154 individuals from two B1 populations of Brassica rapa (syn. Brassica campestris) was evaluated under controlled conditions in a greenhouse. The anther orientation was evaluated visually at the time of fully open flowers. The observed extrorse:introrse ratios were 1 : 1 in R-o-18 × (R-o-18 × RM29) population and 3 : 1 in R-o-18 × (R-o-18 × R c-50) population. It was concluded that this trait is controlled by two duplicated pairs of genes (A1, A2) for extrorse anthers, either of which can produce extrorse anthers when a single dominant allele is present. Introrse anthers result when all alleles at both loci are recessive.

Research paper thumbnail of Testing of susceptibility of horseweed (Conyza canadensis L. (Cronq.)) to glyphosate after its long term use in two railway stations

Research paper thumbnail of Assessment of genetic diversity in sorghum bicolor using RAPD markers

Genetika, 2019

Sorghum is one of the most important cereal crop and ranks fifth among cereals after wheat, rice,... more Sorghum is one of the most important cereal crop and ranks fifth among cereals after wheat, rice, maize and barley for economic importance. Because the demand of food is increasing, sorghum will increase in importance as a source of food, feed, fibre, and fuel; specially in the European continent where sorghum is little cultivated, mainly due to the lack of sorghum varieties well adapted to the soil and climate conditions such as photoperiod, cold and drought; for this reason, the genetic diversity analysis, through molecular characterization, is an important requirement to begin a plant breeding program. The analysis was performed in 46 sorghum genotypes obtained from the Czech Plant Gene Bank, Crop Research Institute, Prague. Genetic variability values were estimated, through the genetic distance using Dice's coefficient, and dendrogram constructed using DARwing software. Four out of fifteen of the primers evaluated were completely polymorphic (100%), A hundred and twenty-six scorable bands were identified and 89% of them were polymorphic, the bands ranged from 200 to 2000 bp. The dendrogram grouped the accession into six 790

Research paper thumbnail of Effect of temperature on severity of Fusarium wilt of cabbage caused by Fusarium oxysporum f. sp. conglutinans

European Journal of Plant Pathology, 2019

Research paper thumbnail of Origin, mechanism and molecular basis of weed resistance to herbicides

Plant Protection Science, 2010

This review summarises information from the literature and experimental experience of the authors... more This review summarises information from the literature and experimental experience of the authors in research on weed resistance to herbicides. Factors conditioning the origin of resistance are described. The origin of resistant weeds to nine active ingredients with a different mode of action is presented chronologically, and the distribution of resistant weeds around the world outlined. The fundamental modes of action: reduction of the target site sensitivity, so-called "target site resistance", and the mode by which a herbicide is metabolised into inactive products, are listed. Function and genetic modifications of target sites of selected herbicides are described. Czech biotypes of resistant weeds with a mutation at codon 264 of the psbA gene encoding the D1 protein and at codon 574 of the acetolactate synthase gene are presented.

Research paper thumbnail of Selecting with markers linked to the PPVres major QTL is not sufficient to predict resistance to Plum Pox Virus (PPV) in apricot

Tree Genetics & Genomes, 2014

ABSTRACT Sharka is one of the most serious viral diseases affecting stone fruit species and, in a... more ABSTRACT Sharka is one of the most serious viral diseases affecting stone fruit species and, in apricot, resistance to its viral agent, the Plum Pox Virus (PPV), is conferred by one major quantitative trait locus (QTL), named PPVres for PPV resistance. Previous studies indicated that PPV-resistant cultivars and breeding progenies can be selected by using a set of SSR markers (named PGS) targeting the PPVres locus. However, before these markers can be employed for markerassisted selection, they were validated in a wide range of genetic backgrounds and environments. We used a total of 11 mapping populations issued from three distinct environments to confirm that this marker set located within the QTL adequately predicted PPV resistance. In this study, we show that selection of PPV-resistant material based only on markers co-localizing with the PPVres major locus is not fully reliable. Indeed, genotype-phenotype discrepancies were observed depending on the progeny and the PPV-resistant/susceptible parents.While most of the PPV-resistant individuals displayed the resistant alleles, a significant number of PPV-susceptible individuals showed the same resistant haplotype. An effect of the PPV strain used for phenotyping was also demonstrated. We thus hypothesize that the presence of other factors or genes involved in the mechanism of resistance to sharka in apricot could explain these unexpected results. Our work indicates that the current PGS marker set is not broadly applicable for MAS and that marker-assisted breeding based on the sole PPVres locus is not sufficient to unambiguously select PPVresistant apricot cultivars.

Research paper thumbnail of Quantitative trait loci meta-analysis of Plum pox virus resistance in apricot ( Prunus armeniaca L.): new insights on the organization and the identification of genomic resistance factors

Molecular Plant Pathology, 2009

Research paper thumbnail of Evaluation of the Prunus interspecific progenies for resistance to Plum pox virus

Czech Journal of Genetics and Plant Breeding, 2013

Sharka disease caused by the infection with the Plum pox virus (PPV) in stone fruit trees is worl... more Sharka disease caused by the infection with the Plum pox virus (PPV) in stone fruit trees is worldwide the most devastating for stone fruit production. Until now, good sources of resistance to PPV within the peach group have not been available. There are no commercial cultivars of peach that are resistant to PPV. Other Prunus species are known to show varying levels of resistance. Interspecific hybrids GF 677 (Prunus amygdalus × P. persica) and Cadaman (P. davidiana × P. persica) were revealed to be resistant to PPV. The resistance to a Dideron isolate of the descendants of Cresthaven × GF 677 and Cresthaven × Cadaman and their progenitors was evaluated after inoculation by chip-budding in a sealed screenhouse. Results demonstrate a certain level of resistance in both progenies of interspecific hybrids and indicate a potential for PPV resistance transfer to commercial peach cultivars but it will be necessary to perform backcrosses with peach cultivars of agri...

Research paper thumbnail of Oligogenic Inheritance of Resistance to Plum Pox Virus in Apricots

Czech Journal of Genetics and Plant Breeding

In order to determine the inheritance of resistance to PPV in apricot three crosses between resis... more In order to determine the inheritance of resistance to PPV in apricot three crosses between resistant and susceptible cultivars and selections were performed. The B<sub>1</sub> seedlings were inoculated with the PPV-M strain by an infected bud. PPV infection was evaluated over 5 consecutive growth periods through visual symptoms, ELISA and in some cases reverse transcriptase PCR assays. Chi-square analysis of each B<sub>1</sub> progeny was performed to determine if the segregation ratio differed from the expected ratio. PPV resistance segregated in three apricot B<sub>1</sub>progenies in a 1:7 (resistant:susceptible) ratio, indicating that resistance was controlled by three independent dominant complementary genes. All three dominant genes are needed for the resistance to be expressed, and the lack of any one of the dominant alleles will result in susceptibility. This knowledge will help us in effective planning of apricot breeding programs with t...

Research paper thumbnail of Analysis of Ribosomal Dna Sequences of Synchytrium Endobioticum (Schilberzsky) Percival

... QC0048). References ALTSCHUL, SF – MADDEN, TL – SCHÄFFER, AA – ZHANG, J. – ZHANG, Z. – MILLER... more ... QC0048). References ALTSCHUL, SF – MADDEN, TL – SCHÄFFER, AA – ZHANG, J. – ZHANG, Z. – MILLER, W. AND LIPMAN, DJ 1997. ... Humana Press, Totowa, NJ. 2000, p. 365-386. WARD, TJ – ADAMS, MJ – MUTASA, ES – COLLIER, CR and ASHER, MJC 1994. ...

Research paper thumbnail of Molecular Study on Acetolactate Synthase Gene (Als) of Two Czech Biotypes of Kochia (Kochia Scoparia (L.) SCHRAD.)

Research paper thumbnail of The Present State of Herbicide Resistance of Weed Populations in the Czech Republic

Journal of Plant Protection Research, 2007

Research paper thumbnail of Origin and diversity of the sources of resistance to PPV in apricot: implications and use in marker-assisted resistance to sharka

HAL (Le Centre pour la Communication Scientifique Directe), Sep 3, 2013

HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

Research paper thumbnail of A genetic linkage map for an apricot (Prunus armeniaca L.) BC1 population mapping plum pox virus resistance

Tree Genetics & Genomes, 2008

Plum pox virus (sharka; PPV) can cause severe crop loss in economically important Prunus species ... more Plum pox virus (sharka; PPV) can cause severe crop loss in economically important Prunus species such as peach, plum, apricot, and cherry. Of these species, certain apricot cultivars ('Stark Early Orange', 'Goldrich', 'Harlayne') display significant levels of resistance to the disease and are the genetic substrate for studies of several xlaboratories working cooperatively to genetically characterize and mark the resistance locus or loci for marker-assisted breeding. The goals of the work presented in this communication are the characterization of the genetics of PPV resistance in 'Stark Early Orange' and the development of co-dominant molecular markers for marker-assisted selection (MAS) in PPV resistance breeding. We present the first genetic linkage map for an apricot backcross population of 'Stark Early Orange' and the susceptible cultivar 'Vestar' that segregates for resistance to PPV. This map is comprised of 357 loci (330 amplified fragment length polymorphisms (AFLPs), 26 simple sequence repeats (SSRs), and 1 morphological marker for PPV resistance) assigned to eight linkage groups. Twentytwo of the mapped SSRs are shared in common with genetic reference map for Prunus (T × E; Joobeur et al. 1998) and anchor our apricot map to the general Prunus map. A PPV resistance locus was mapped in linkage group 1 and four AFLP markers segregating with the PPV resistance trait, identified through bulk segregant analysis, facilitated the development of SSRs in this region.

Research paper thumbnail of Construction of a Genetic Map for Apricot with Molecular Markers and Identification of Markers Associated with Plum Pox Virus Resistance

International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species, 2007

Plum pox virus (PPV) causes serious damage in apricots and breeding and growing of PPV-resistant ... more Plum pox virus (PPV) causes serious damage in apricots and breeding and growing of PPV-resistant apricot cultivars are the only way of its efficient control. An integrated genetic linkage map for apricot (Prunus armeniaca L.) was obtained using a BC 1 progeny 'LE-3246' x 'Vestar'. A total of 316 molecular markers (290 AFLPs, 26 SSRs) and the resistance to PPV were assigned to 8 linkage groups covering 574 cM of the apricot genome. The average distance between adjacent loci is 2.5 cM. A PPVres1 locus conditioning resistance to PPV was mapped in linkage group 1. Several AFLPs and an SSR tightly linked to PPVres1 locus were identified. The construction of a genetic map for apricot and identification of molecular markers linked to PPV resistance will facilitate the breeder to obtain resistant apricot cultivars.

Research paper thumbnail of Genetic Diversity between Yacon Landraces from Different Countries Based on Random Amplified Polymorphic DNAs

Czech Journal of Genetics and Plant Breeding, 2011

Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relati... more Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relationship of crop germplasm. Five different landraces of yacon (Smallantus sonchifolius (Poepp. and Hendl.) H. Robinson; Asteraceae) collected in various countries and showing different morphological traits were investigated using a total of 61 decamer primers. A total of 282 RAPD markers were scored and 28.7% of them were polymorphic at least within landraces. RAPD markers generated by one primer (OBP14) discriminated between all landraces. Markers were used to calculate genetic similarity coefficient and to build a dendrogram representing the genetic relationship between analysed landraces. The results suggest that RAPD markers could be used as a reliable tool to perform fingerprinting studies in Smallantus sonchifolius genome. This is the first report on the use of RAPDs to evaluate genetic distance and to distinguish between different landraces in yacon.

Research paper thumbnail of Application of molecular markers in breeding for resistance to the Plum Pox Virus in Prunus spp

HAL (Le Centre pour la Communication Scientifique Directe), Jan 13, 2007

Research paper thumbnail of The emergence of a atrazine resistant black nightshade (Solanum nigrum L.) biotype and molecular basis of the resistance

Plant Protection Science, 2010

Seeds from atrazine resistant plants of black nightshade (<I>Solanum nigrum </I>L.)&l... more Seeds from atrazine resistant plants of black nightshade (<I>Solanum nigrum </I>L.)<I> </I>were collected at the railway station Prague-Vršovice, seeds from susceptible plants in Vyšehořovice (Prague East district). Tests on emergence showed that in both resistant and susceptible biotypes it was highest at a seeding depth of 1 mm, and that at the same seeding depth there were statistically significant differences in emergence between the resistant and susceptible biotypes. The resistance or susceptibility to atrazine was tested by both a chlorophyll fluorescence assay and spraying with atrazine. A region of the gene encoding D1 protein of photosystem II was sequenced and compared between the resistant and susceptible biotypes. Resistance to atrazine in the <I>S. nigrum</I> biotype from Vršovice was conferred by a glycine for serine substitution at residue 264 of the D1 protein. In the plants of the biotypes there was excellent correspondence betwe...

Research paper thumbnail of Changes in baking quality of winter wheat with different intensity of Fusarium spp. contamination detected by means of new rheological system

Czech Journal of Food Sciences, 2011

The aim of our work was to assess the possibility of detecting the changes in the baking quality ... more The aim of our work was to assess the possibility of detecting the changes in the baking quality of winter wheat with different levels of Fusarium spp. contamination using a new rheological system Mixolab, and to determine the correlations between the Mixolab characteristics and other quality parameters of wheat flour and grain. The standard technological characteristics (crude protein, Zeleny sedimentation index, wet gluten, falling number), loaf volume, shape features of bread (height and diameter), Mixolab parameters, and mycotoxin deoxynivalenol (DON) content were determined in 3 winter wheat cultivars (Akteur – quality group E – elite; Eurofit – quality group A; Meritto – quality group B) with different levels of Fusarium spp. contamination (8 variants) in two years. Increasing intensity of Fusarium spp. contamination evidently worsened the rheological quality and its negative effects on protein and mainly on the starch part of the grain was obvious in Mixolab curves. High corr...

Research paper thumbnail of Methodology for the phenotyping resistance to Plum pox virus in peach

Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important v... more Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important virus disease in fruit trees worldwide. Since it is not possible to completely eradicate PPV in the Czech Republic, it is necessary to find another way to control this pathogen. The most promising seems to be the breeding of new cultivars for resistance. This methodology provides a comprehensive protocol for testing PPV resistance in peach. It is based on visual assessment of disease symptoms on leaves and fruits, and determining the presence of the virus in plants using ELISA and RT-PCR techniques

Research paper thumbnail of Mendelian inheritance of introrse orientated anthers in Brassica rapa

Czech Journal of Genetics and Plant Breeding, 2022

The inheritance of anther orientation of 154 individuals from two B1 populations of Brassica rapa... more The inheritance of anther orientation of 154 individuals from two B1 populations of Brassica rapa (syn. Brassica campestris) was evaluated under controlled conditions in a greenhouse. The anther orientation was evaluated visually at the time of fully open flowers. The observed extrorse:introrse ratios were 1 : 1 in R-o-18 × (R-o-18 × RM29) population and 3 : 1 in R-o-18 × (R-o-18 × R c-50) population. It was concluded that this trait is controlled by two duplicated pairs of genes (A1, A2) for extrorse anthers, either of which can produce extrorse anthers when a single dominant allele is present. Introrse anthers result when all alleles at both loci are recessive.

Research paper thumbnail of Testing of susceptibility of horseweed (Conyza canadensis L. (Cronq.)) to glyphosate after its long term use in two railway stations

Research paper thumbnail of Assessment of genetic diversity in sorghum bicolor using RAPD markers

Genetika, 2019

Sorghum is one of the most important cereal crop and ranks fifth among cereals after wheat, rice,... more Sorghum is one of the most important cereal crop and ranks fifth among cereals after wheat, rice, maize and barley for economic importance. Because the demand of food is increasing, sorghum will increase in importance as a source of food, feed, fibre, and fuel; specially in the European continent where sorghum is little cultivated, mainly due to the lack of sorghum varieties well adapted to the soil and climate conditions such as photoperiod, cold and drought; for this reason, the genetic diversity analysis, through molecular characterization, is an important requirement to begin a plant breeding program. The analysis was performed in 46 sorghum genotypes obtained from the Czech Plant Gene Bank, Crop Research Institute, Prague. Genetic variability values were estimated, through the genetic distance using Dice's coefficient, and dendrogram constructed using DARwing software. Four out of fifteen of the primers evaluated were completely polymorphic (100%), A hundred and twenty-six scorable bands were identified and 89% of them were polymorphic, the bands ranged from 200 to 2000 bp. The dendrogram grouped the accession into six 790

Research paper thumbnail of Effect of temperature on severity of Fusarium wilt of cabbage caused by Fusarium oxysporum f. sp. conglutinans

European Journal of Plant Pathology, 2019

Research paper thumbnail of Origin, mechanism and molecular basis of weed resistance to herbicides

Plant Protection Science, 2010

This review summarises information from the literature and experimental experience of the authors... more This review summarises information from the literature and experimental experience of the authors in research on weed resistance to herbicides. Factors conditioning the origin of resistance are described. The origin of resistant weeds to nine active ingredients with a different mode of action is presented chronologically, and the distribution of resistant weeds around the world outlined. The fundamental modes of action: reduction of the target site sensitivity, so-called "target site resistance", and the mode by which a herbicide is metabolised into inactive products, are listed. Function and genetic modifications of target sites of selected herbicides are described. Czech biotypes of resistant weeds with a mutation at codon 264 of the psbA gene encoding the D1 protein and at codon 574 of the acetolactate synthase gene are presented.

Research paper thumbnail of Selecting with markers linked to the PPVres major QTL is not sufficient to predict resistance to Plum Pox Virus (PPV) in apricot

Tree Genetics & Genomes, 2014

ABSTRACT Sharka is one of the most serious viral diseases affecting stone fruit species and, in a... more ABSTRACT Sharka is one of the most serious viral diseases affecting stone fruit species and, in apricot, resistance to its viral agent, the Plum Pox Virus (PPV), is conferred by one major quantitative trait locus (QTL), named PPVres for PPV resistance. Previous studies indicated that PPV-resistant cultivars and breeding progenies can be selected by using a set of SSR markers (named PGS) targeting the PPVres locus. However, before these markers can be employed for markerassisted selection, they were validated in a wide range of genetic backgrounds and environments. We used a total of 11 mapping populations issued from three distinct environments to confirm that this marker set located within the QTL adequately predicted PPV resistance. In this study, we show that selection of PPV-resistant material based only on markers co-localizing with the PPVres major locus is not fully reliable. Indeed, genotype-phenotype discrepancies were observed depending on the progeny and the PPV-resistant/susceptible parents.While most of the PPV-resistant individuals displayed the resistant alleles, a significant number of PPV-susceptible individuals showed the same resistant haplotype. An effect of the PPV strain used for phenotyping was also demonstrated. We thus hypothesize that the presence of other factors or genes involved in the mechanism of resistance to sharka in apricot could explain these unexpected results. Our work indicates that the current PGS marker set is not broadly applicable for MAS and that marker-assisted breeding based on the sole PPVres locus is not sufficient to unambiguously select PPVresistant apricot cultivars.

Research paper thumbnail of Quantitative trait loci meta-analysis of Plum pox virus resistance in apricot ( Prunus armeniaca L.): new insights on the organization and the identification of genomic resistance factors

Molecular Plant Pathology, 2009

Research paper thumbnail of Evaluation of the Prunus interspecific progenies for resistance to Plum pox virus

Czech Journal of Genetics and Plant Breeding, 2013

Sharka disease caused by the infection with the Plum pox virus (PPV) in stone fruit trees is worl... more Sharka disease caused by the infection with the Plum pox virus (PPV) in stone fruit trees is worldwide the most devastating for stone fruit production. Until now, good sources of resistance to PPV within the peach group have not been available. There are no commercial cultivars of peach that are resistant to PPV. Other Prunus species are known to show varying levels of resistance. Interspecific hybrids GF 677 (Prunus amygdalus × P. persica) and Cadaman (P. davidiana × P. persica) were revealed to be resistant to PPV. The resistance to a Dideron isolate of the descendants of Cresthaven × GF 677 and Cresthaven × Cadaman and their progenitors was evaluated after inoculation by chip-budding in a sealed screenhouse. Results demonstrate a certain level of resistance in both progenies of interspecific hybrids and indicate a potential for PPV resistance transfer to commercial peach cultivars but it will be necessary to perform backcrosses with peach cultivars of agri...

Research paper thumbnail of Oligogenic Inheritance of Resistance to Plum Pox Virus in Apricots

Czech Journal of Genetics and Plant Breeding

In order to determine the inheritance of resistance to PPV in apricot three crosses between resis... more In order to determine the inheritance of resistance to PPV in apricot three crosses between resistant and susceptible cultivars and selections were performed. The B<sub>1</sub> seedlings were inoculated with the PPV-M strain by an infected bud. PPV infection was evaluated over 5 consecutive growth periods through visual symptoms, ELISA and in some cases reverse transcriptase PCR assays. Chi-square analysis of each B<sub>1</sub> progeny was performed to determine if the segregation ratio differed from the expected ratio. PPV resistance segregated in three apricot B<sub>1</sub>progenies in a 1:7 (resistant:susceptible) ratio, indicating that resistance was controlled by three independent dominant complementary genes. All three dominant genes are needed for the resistance to be expressed, and the lack of any one of the dominant alleles will result in susceptibility. This knowledge will help us in effective planning of apricot breeding programs with t...

Research paper thumbnail of Analysis of Ribosomal Dna Sequences of Synchytrium Endobioticum (Schilberzsky) Percival

... QC0048). References ALTSCHUL, SF – MADDEN, TL – SCHÄFFER, AA – ZHANG, J. – ZHANG, Z. – MILLER... more ... QC0048). References ALTSCHUL, SF – MADDEN, TL – SCHÄFFER, AA – ZHANG, J. – ZHANG, Z. – MILLER, W. AND LIPMAN, DJ 1997. ... Humana Press, Totowa, NJ. 2000, p. 365-386. WARD, TJ – ADAMS, MJ – MUTASA, ES – COLLIER, CR and ASHER, MJC 1994. ...

Research paper thumbnail of Molecular Study on Acetolactate Synthase Gene (Als) of Two Czech Biotypes of Kochia (Kochia Scoparia (L.) SCHRAD.)

Research paper thumbnail of The Present State of Herbicide Resistance of Weed Populations in the Czech Republic

Journal of Plant Protection Research, 2007