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![Research paper thumbnail of Interaction of the [PtCl2(DMSO)2] Complex with L-Cysteine](https://attachments.academia-assets.com/43586772/thumbnails/1.jpg)
](Zeitschrift für Naturforschung C, 2009
The reaction between [PtCl 2 (DMSO) 2 ] and L-cysteine (L-Cys) has been investigated in the prese... more The reaction between [PtCl 2 (DMSO) 2 ] and L-cysteine (L-Cys) has been investigated in the presence of micelles of sodium dodecyl sulfate (SDS) -as a model for biological membranes. Additionally, the inhibitory effect of [PtCl 2 (DMSO) 2 ] on the Na + ,K + -ATPase activity and its partial prevention with 10 mM L-Cys were demonstrated. The interaction of L-Cys with [PtCl 2 (DMSO) 2 ] resulted in the formation of a [Pt(DMSO) 2 (L-Cys) 2 ] 2+ (DMSO) 2 ] complex, which most probably occurs through stepwise replacement of Clwith L-Cys. It has also been demonstrated that neither the pH value nor SDS affects the composition of the new complex. On the other hand, the pH value and SDS do affect the reaction rate, most probably due to electrostatic interactions with reactants. In summary, this study can be used as a simple model approach for the investigation of reaction mechanisms between platinum complexes and various biomolecules, and for the determination of potential toxicity and/or side effects of antitumour platinum drugs.
Rapid Communications in Mass Spectrometry, 2009
Attempts are being made to overcome the resistance of tumour cells to platinum (Pt) drugs by the ... more Attempts are being made to overcome the resistance of tumour cells to platinum (Pt) drugs by the synthesis of new generations of Pt complexes, and it is important to find appropriate and simple methods for the characterization of those novel complexes. The additional applicability of such a method for the analysis of the interactions of metal complexes with biomolecules would be advantageous. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) seems to possess the capability to become this method of choice, since it could be applied to low-mass complexes as well as for the analysis of large biomolecules. In this work the applicability of flavonoids -quercetin and rutin -as matrices for MALDI-TOFMS analysis of dichlorido(ethylendiamine)platinum(II) ([PtCl 2 (en)]), dichlorido(diaminocyclohexane)platinum(II) ([PtCl 2 (dach)]) and chloride (diethylenetriamine) palladium(II) chloride ([PdCl(dien)]Cl) complexes is demonstrated. Spectra of Pt(II) and Pd(II) complexes recorded in the presence of quercetin and rutin are rather simple: Pt(II) complexes generate [MRNa] R or [MRK] R ions, whereas the investigated Pd(II) complex gives ions generated by the loss of one Cl À or HCl. Flavonoids give a relatively small number of well-defined ions in the low-mass region (at m/z 303.3 for quercetin and m/z 633.5 for rutin). Quercetin and rutin can be applied in much lower concentrations than other common MALDI matrices and require rather low laser intensity. We speculate that flavonoids stabilize the structures of the metal complexes and that they may be useful for the analysis of other biologically active metal complexes, thus implying their broader applicability.
Russian Journal of Physical Chemistry A, 2009
Zeitschrift für Naturforschung C, 2009
The reaction between [PtCl 2 (DMSO) 2 ] and L-cysteine (L-Cys) has been investigated in the prese... more The reaction between [PtCl 2 (DMSO) 2 ] and L-cysteine (L-Cys) has been investigated in the presence of micelles of sodium dodecyl sulfate (SDS) -as a model for biological membranes. Additionally, the inhibitory effect of [PtCl 2 (DMSO) 2 ] on the Na + ,K + -ATPase activity and its partial prevention with 10 mM L-Cys were demonstrated. The interaction of L-Cys with [PtCl 2 (DMSO) 2 ] resulted in the formation of a [Pt(DMSO) 2 (L-Cys) 2 ] 2+ (DMSO) 2 ] complex, which most probably occurs through stepwise replacement of Clwith L-Cys. It has also been demonstrated that neither the pH value nor SDS affects the composition of the new complex. On the other hand, the pH value and SDS do affect the reaction rate, most probably due to electrostatic interactions with reactants. In summary, this study can be used as a simple model approach for the investigation of reaction mechanisms between platinum complexes and various biomolecules, and for the determination of potential toxicity and/or side effects of antitumour platinum drugs.
Rapid Communications in Mass Spectrometry, 2009
Attempts are being made to overcome the resistance of tumour cells to platinum (Pt) drugs by the ... more Attempts are being made to overcome the resistance of tumour cells to platinum (Pt) drugs by the synthesis of new generations of Pt complexes, and it is important to find appropriate and simple methods for the characterization of those novel complexes. The additional applicability of such a method for the analysis of the interactions of metal complexes with biomolecules would be advantageous. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) seems to possess the capability to become this method of choice, since it could be applied to low-mass complexes as well as for the analysis of large biomolecules. In this work the applicability of flavonoids -quercetin and rutin -as matrices for MALDI-TOFMS analysis of dichlorido(ethylendiamine)platinum(II) ([PtCl 2 (en)]), dichlorido(diaminocyclohexane)platinum(II) ([PtCl 2 (dach)]) and chloride (diethylenetriamine) palladium(II) chloride ([PdCl(dien)]Cl) complexes is demonstrated. Spectra of Pt(II) and Pd(II) complexes recorded in the presence of quercetin and rutin are rather simple: Pt(II) complexes generate [MRNa] R or [MRK] R ions, whereas the investigated Pd(II) complex gives ions generated by the loss of one Cl À or HCl. Flavonoids give a relatively small number of well-defined ions in the low-mass region (at m/z 303.3 for quercetin and m/z 633.5 for rutin). Quercetin and rutin can be applied in much lower concentrations than other common MALDI matrices and require rather low laser intensity. We speculate that flavonoids stabilize the structures of the metal complexes and that they may be useful for the analysis of other biologically active metal complexes, thus implying their broader applicability.
Russian Journal of Physical Chemistry A, 2009