DrJayapal M.R - Academia.edu (original) (raw)
Papers by DrJayapal M.R
The Southeast Asian journal of tropical medicine and public health
Two major factors, higher temperatures and the application of insecticides, can drastically alter... more Two major factors, higher temperatures and the application of insecticides, can drastically alter the genetic structure of a vector mosquito population. Due to these two stresses, the majority of the population gets wiped out, but the ones that withstand the stress and survive are likely to pass on survivability, and have an altered physiology. Our study shows that exposures to higher temperatures and DDT during the larval stage affects their susceptibility as adult mosquitoes to the DEN-2 virus. The overall transcription and translation status of heat shock protein (Hsp70) in virus high- and low-susceptible was the same as that in other batches. In the case of a DDT-resistant (R-7) strain two bands were obtained during RT-PCRs after heat shock. These two alleles were obtained only with HY-1 in which R-7 males were used for the crosses, suggesting that the second allele is probably male sex linked. The higher expression of Hsp70 may provide DDT-resistant strains a better chance of s...
IETE Journal of Research, 1995
2009 International Conference on Industrial and Information Systems (ICIIS), 2009
Abstract-In this paper a four stage character/bit level encoding technique (RBCMCPCC) has been pr... more Abstract-In this paper a four stage character/bit level encoding technique (RBCMCPCC) has been proposed, where the first three steps take input block of length 128 bit, 192bit and 256 bit, respectively, and generate intermediate blocks of the same lengths using identical ...
Statistica Sinica
The isotonic regression problem with a smoothness penalty is considered. The shape-restricted smo... more The isotonic regression problem with a smoothness penalty is considered. The shape-restricted smooth estimator was characterized as a solution to a set of recurrence relations by Tantiyaswasdikul and Woodroofe (1994). Using a related Green's function, the estimator can be represented as a kernel regression estima-tor. Under regularity conditions on the underlying regression function, asymptotic normality of the estimator is established for a large range of choices of the tuning parameter.
There have been recent advances in the nonparametric estimation of the distribution of dark matte... more There have been recent advances in the nonparametric estimation of the distribution of dark matter in nearby dwarf spheroidal galaxies. This work uses Jeans' Equations to relate the distribution of (total) matter to velocity dispersions. The estimation of velocity dispersions is facilitated by observing certain shape restrictions, stronger that convexity in the isotropic case, that follow from Jeans' Equations. This work is reviewed, modified slightly, and extended to include some anisotropy.
Free Radical Research
Oxidative stress leads to perturbation of a variety of cellular processes resulting in inhibition... more Oxidative stress leads to perturbation of a variety of cellular processes resulting in inhibition of cell proliferation. This study has determined the effect of oxidative stress on protein synthesis in human K562 cells using a hydrophilic peroxyl radical initiator, AAPH and H(2)O(2). The results indicated that oxidative stress leads to a significant decrease in the rate of protein synthesis caused due to induced activation as well as expression of the erythroid cell-specific eIF-2alpha kinase, called the Heme Regulated Inhibitor (HRI). Elevated levels of HRI expression and activity were accompanied by increased lipid peroxidation and decreased cell proliferation. Further, oxidative stress also caused inactivation of p34(cdc2) kinase, thereby arresting cell division leading to apoptosis. Thus, the data provides the mechanism of inhibition of protein synthesis and perturbation of a cell cycle regulatory protein leading to inhibition of cell proliferation in K562 cells during oxidative...
The International Journal of Biochemistry & Cell Biology
The heme-regulated inhibitor (HRI), a regulator of translation initiation, is known to be activat... more The heme-regulated inhibitor (HRI), a regulator of translation initiation, is known to be activated and upregulated, and it acts as either a cytoprotective player promoting cell survival or as an inducer of apoptosis during stress. However, the exact role of HRI in these two responses has not been elucidated. In the present investigation, using human cell lines, we attempted to unravel the molecular mechanism(s) of HRI-mediated differential response and the involved signalling pathways. While during low dose (5μM) lead acetate treatment, cells did not show any diminished cell survival, significant level of apoptosis was observed at high dose (100μM) lead acetate. Based on the results of an interactome analysis, we determined the interaction of HRI with PI-3-Kca, only at a low dose stress, which is followed by phosphorylation and activation of its downstream target, AKT. Interestingly, such an interaction and AKT activation was not observed at a high dose stress. On the other hand, a...
Indian journal of biochemistry & biophysics
The heme-regulated inhibitor (HRI), a member of the eIF-2 alpha kinase family is crucial for regu... more The heme-regulated inhibitor (HRI), a member of the eIF-2 alpha kinase family is crucial for regulating protein synthesis during stress. In addition to heme, stress proteins Hsp90 and Hsp70 are known to regulate HRI. The present study aims to determine the physical association of these Hsps in the regulation of HRI activation during oxidative stress using human K562 cells as a model. Extracts from the stress-induced cells were used for determining HRI kinase activity by measuring eIF-2 alpha phosphorylation, and Hsp-HRI interaction by immunoprecipitation and immunoblot analyses. The results indicate a significant increase in both Hsp70 and Hsp90 expression during AAPH (2,2'-azobis (2-amidinopropane) dihydrochloride)-induced oxidative stress. Further, their interaction with HRI, which correlates well with its increased HRI kinase activity leads to inhibition of protein synthesis. Thus, we demonstrate that Hsps play an important role in the regulation of initiation of protein synt...
Pesticide Biochemistry and Physiology
The effect of sublethal concentrations, 0.00141% (LC20), 0.00251% (LC30), and 0.0036% (LC40) of a... more The effect of sublethal concentrations, 0.00141% (LC20), 0.00251% (LC30), and 0.0036% (LC40) of a dispersible formulation of an acylurea insect growth regulator, flufenoxuron (Cascade) on certain biochemical parameters in the larvae of Tribolium castaneum was investigated. When neonates were fed on diet treated with sublethal concentrations for 24 h, it was observed that at all concentrations tested, there was a significant reduction in chitin content on the 15th day of development. Total soluble protein content at LC20 and LC30 decreased with increasing age of the larvae. At LC20 and LC40 concentrations there was a progressive increase in the protein: chitin ratio as a function of increase in age of the larvae. SDS–PAGE analysis of the larval tissue extracts indicated gross quantitative changes in some of the protein bands (MW 50–97 kDa). Western blot analysis revealed significant increase in the level of HSP70 in the extracts of larvae fed on LC30 treated diet, on the 7th and 10th...
The international journal of biochemistry & cell biology
Erythropoiesis is controlled by a complex interplay of several signalling pathways and key transc... more Erythropoiesis is controlled by a complex interplay of several signalling pathways and key transcription factors, as well as microRNAs (miRNAs). MicroRNAs function as critical modulators of gene expression for cellular processes. In the present study, we found that miR-320a inhibits erythroid differentiation by targeting Matrix Attachment Region binding protein SMAR1. miR-320a negatively regulates the expression of SMAR1 by directly binding to its 3'UTR. In response to mild DNA damage, miR-320a expression is decreased resulting in enhanced expression of SMAR1 protein, which in turn, reduces its targets, Bax and Puma inhibiting apoptosis. Our data demonstrate that during hemin-induced erythroid differentiation, enhanced expression of SMAR1 negatively correlates with miR-320a expression. Further analysis reveals that SMAR1 regulates erythroid differentiation, by binding to the promoter of miR-221/222, which play a crucial role in early erythropoiesis. Overall, our studies provide ...
Communications in Computer and Information Science, 2010
N. Meghanathan et al. (Eds.): NeCoM, WiMoN, and WeST 2010, CCIS 90, pp. 124133, 2010. © Springer... more N. Meghanathan et al. (Eds.): NeCoM, WiMoN, and WeST 2010, CCIS 90, pp. 124133, 2010. © Springer-Verlag Berlin Heidelberg 2010 ... A Novel Visual Cryptographic Technique through Grey ... Jayanta Kumar Pal1, JK Mandal2, and Kousik Dasgupta1
2006 IEEE International Conference on Industrial Technology, 2006
Abstract The work intends to obtain the model reduction using balanced singular perturbation appr... more Abstract The work intends to obtain the model reduction using balanced singular perturbation approximation (BSPA) of any LTI system, which may be utilized for controller reduction as well. The MATLAB based computational difficulties for obtaining balanced realized minimal order model of the system necessary for preservation of DC gain of non-minimal systems have been investigated. The algorithm is illustrated by an example showing MATLAB based actual computational difficulties being faced by the researchers ...
Indian journal of biochemistry & biophysics, 1995
Antisera prepared against total water-soluble lens proteins of the shark, Scoliodon sorrakowah we... more Antisera prepared against total water-soluble lens proteins of the shark, Scoliodon sorrakowah were reacted with homologous antigen and analysed reaction products by immunoelectrophoresis (IE) and two dimensional crossed antigen-antibody electrophoresis (2D-CE). On IE, shark antigens formed 5 precipitin lines including 1 alpha, 3 beta and 1 gamma crystallins and on 2D-CE 3 alpha, 6 beta and 6 gamma peaks accounting for 8%, 27% and 65% antigen in the respective group were obtained from the total crystallins. Using anti-shark antisera, the immunocrossreactivity of lens proteins from 6 Chondropterygii, 23 teleosts and 16 higher vertebrates was examined by IE. It is found that beta crystallins are the most conserved and crossreact with all vertebrate classes, whereas gamma crystallin crossreactivity is specific to the class Pisces and alpha crystallins are least conserved and their crossreactivity is confined to subclass Chondropterygii. Based on IE patterns, a phylogenetic tree is cons...
Journal of cell science, 1988
The prosomes, a novel type of small RNA-protein complex previously characterized in avian and mam... more The prosomes, a novel type of small RNA-protein complex previously characterized in avian and mammalian cells, were isolated from axolotl (Ambystoma mexicanum) oocytes and identified by sedimentation analysis and protein composition. The prosomal nature of these particles was further ascertained by immunoblot analysis with anti-duck prosome monoclonal antibodies. By in vitro [35S]methionine labelling, de novo synthesis of prosomal proteins could be detected neither during oogenesis nor meiotic maturation. The results obtained by both indirect immunofluorescence and immunoblot analyses demonstrated a dramatic change in the localization of prosomal antigens during oocyte development. They were initially detected in the oocyte cytoplasm, during oocyte growth. At the end of vitellogenesis (stages V-VI), they entered the nucleus (germinal vesicle) and were accumulated there to the highest concentration. During oocyte maturation, after nuclear envelope breakdown, prosomal antigens were fo...
The Journal of Biochemistry, Molecular Biology and Biophysics, 2002
We have used human cell lines, namely, K562 and HeLa cells as model systems in understanding the ... more We have used human cell lines, namely, K562 and HeLa cells as model systems in understanding the mechanism of lead toxicity and heat shock, that may be mediated by the heme-regulated eIF-2alpha kinase which is also called the heme-regulated inhibitor (HRI). RT-PCR analysis using HRI-specific primers indicated a two- to three-fold increase in HRI expression in K562 and HeLa cells exposed to lead acetate and heat shock, respectively. Further, in vitro eIF-2alpha kinase assay indicated a two- to three-fold increase in HRI kinase activity during lead toxicity in K562 cells. This increase in HRI expression and its activity was accompanied by a significant decrease in cell proliferation and cell viability. This is therefore, the first report indicating that both heavy metal exposure and heat shock cause inhibition of protein synthesis not by activation of HRI alone but by its over-expression as well as activation. Our data indicate further that lead-induced inhibition of cell proliferation may be caused due to inhibition of protein synthesis resulted due to induced expression and activity of HRI.
2006 IEEE International Conference on Industrial Technology, 2006
Abstract In the paper, robust design and tuning of power system stabilizer (PSS) is considered. T... more Abstract In the paper, robust design and tuning of power system stabilizer (PSS) is considered. The objective is to tune a PSS so that the closed-loop response of a single machine-infinite bus system is made not only stable over a wider range of operating points but also the time responses are restricted to lie within specific bounds while requiring minimum control effort. The new robust PSS approach uses the Luus-Jaakola (LJ) optimization procedure in conjunction with genetic algorithm (GA). Simulation results on a ...
2008 16th International Conference on Advanced Computing and Communications, 2008
In this paper, variable block length based character / bit level transformation has been proposed... more In this paper, variable block length based character / bit level transformation has been proposed for encryption, where a block of 16 characters/128 bits has been taken into account. The block is passed through a substitution operation followed by various transpositions using multi dimensional array. The block is operated with its one-time sub key which produces intermediate output of the
Biotechnology and Applied Biochemistry, 2002
The haem-regulated eukaryotic initiation factor 2α kinase, also called the haem-regulated inhibit... more The haem-regulated eukaryotic initiation factor 2α kinase, also called the haem-regulated inhibitor (HRI), has been shown to increase in the peripheral blood cells as a function of drug-induced anaemia in rabbits, suggesting that it could be a molecular indicator of drug-induced anaemia [Anand and Pal (1997) J. Biosci. 22, 287-298]. In the present investigation, we have determined the expression of HRI during lead-induced anaemia in rabbits. The level of anaemia has been determined by routine procedures such as reticulocyte count, haemoglobin content and packed cell volume. These values were compared with the results obtained for a quantitative Western blot of HRI in the blood cell lysates of drug-and lead-induced anaemic rabbits. These results indicate that HRI could be used as a molecular marker for lead-induced anaemia since a progressive increase in HRI levels could be detected as a function of the time of lead exposure. In order to understand the role of stress proteins, heat-shock protein (Hsp) 70 and Hsp90, in inducing anaemia during lead exposure, levels of Hsp70 and Hsp90, and their interaction with HRI, have been determined. Increased levels of these proteins and their intermolecular complexes with HRI suggest their role in regulating protein synthesis during lead-induced anaemia. These observations further reiterate the use of HRI as a potential indicator for drug-and heavy-metal-induced anaemia in humans.
The Southeast Asian journal of tropical medicine and public health
Two major factors, higher temperatures and the application of insecticides, can drastically alter... more Two major factors, higher temperatures and the application of insecticides, can drastically alter the genetic structure of a vector mosquito population. Due to these two stresses, the majority of the population gets wiped out, but the ones that withstand the stress and survive are likely to pass on survivability, and have an altered physiology. Our study shows that exposures to higher temperatures and DDT during the larval stage affects their susceptibility as adult mosquitoes to the DEN-2 virus. The overall transcription and translation status of heat shock protein (Hsp70) in virus high- and low-susceptible was the same as that in other batches. In the case of a DDT-resistant (R-7) strain two bands were obtained during RT-PCRs after heat shock. These two alleles were obtained only with HY-1 in which R-7 males were used for the crosses, suggesting that the second allele is probably male sex linked. The higher expression of Hsp70 may provide DDT-resistant strains a better chance of s...
IETE Journal of Research, 1995
2009 International Conference on Industrial and Information Systems (ICIIS), 2009
Abstract-In this paper a four stage character/bit level encoding technique (RBCMCPCC) has been pr... more Abstract-In this paper a four stage character/bit level encoding technique (RBCMCPCC) has been proposed, where the first three steps take input block of length 128 bit, 192bit and 256 bit, respectively, and generate intermediate blocks of the same lengths using identical ...
Statistica Sinica
The isotonic regression problem with a smoothness penalty is considered. The shape-restricted smo... more The isotonic regression problem with a smoothness penalty is considered. The shape-restricted smooth estimator was characterized as a solution to a set of recurrence relations by Tantiyaswasdikul and Woodroofe (1994). Using a related Green's function, the estimator can be represented as a kernel regression estima-tor. Under regularity conditions on the underlying regression function, asymptotic normality of the estimator is established for a large range of choices of the tuning parameter.
There have been recent advances in the nonparametric estimation of the distribution of dark matte... more There have been recent advances in the nonparametric estimation of the distribution of dark matter in nearby dwarf spheroidal galaxies. This work uses Jeans' Equations to relate the distribution of (total) matter to velocity dispersions. The estimation of velocity dispersions is facilitated by observing certain shape restrictions, stronger that convexity in the isotropic case, that follow from Jeans' Equations. This work is reviewed, modified slightly, and extended to include some anisotropy.
Free Radical Research
Oxidative stress leads to perturbation of a variety of cellular processes resulting in inhibition... more Oxidative stress leads to perturbation of a variety of cellular processes resulting in inhibition of cell proliferation. This study has determined the effect of oxidative stress on protein synthesis in human K562 cells using a hydrophilic peroxyl radical initiator, AAPH and H(2)O(2). The results indicated that oxidative stress leads to a significant decrease in the rate of protein synthesis caused due to induced activation as well as expression of the erythroid cell-specific eIF-2alpha kinase, called the Heme Regulated Inhibitor (HRI). Elevated levels of HRI expression and activity were accompanied by increased lipid peroxidation and decreased cell proliferation. Further, oxidative stress also caused inactivation of p34(cdc2) kinase, thereby arresting cell division leading to apoptosis. Thus, the data provides the mechanism of inhibition of protein synthesis and perturbation of a cell cycle regulatory protein leading to inhibition of cell proliferation in K562 cells during oxidative...
The International Journal of Biochemistry & Cell Biology
The heme-regulated inhibitor (HRI), a regulator of translation initiation, is known to be activat... more The heme-regulated inhibitor (HRI), a regulator of translation initiation, is known to be activated and upregulated, and it acts as either a cytoprotective player promoting cell survival or as an inducer of apoptosis during stress. However, the exact role of HRI in these two responses has not been elucidated. In the present investigation, using human cell lines, we attempted to unravel the molecular mechanism(s) of HRI-mediated differential response and the involved signalling pathways. While during low dose (5μM) lead acetate treatment, cells did not show any diminished cell survival, significant level of apoptosis was observed at high dose (100μM) lead acetate. Based on the results of an interactome analysis, we determined the interaction of HRI with PI-3-Kca, only at a low dose stress, which is followed by phosphorylation and activation of its downstream target, AKT. Interestingly, such an interaction and AKT activation was not observed at a high dose stress. On the other hand, a...
Indian journal of biochemistry & biophysics
The heme-regulated inhibitor (HRI), a member of the eIF-2 alpha kinase family is crucial for regu... more The heme-regulated inhibitor (HRI), a member of the eIF-2 alpha kinase family is crucial for regulating protein synthesis during stress. In addition to heme, stress proteins Hsp90 and Hsp70 are known to regulate HRI. The present study aims to determine the physical association of these Hsps in the regulation of HRI activation during oxidative stress using human K562 cells as a model. Extracts from the stress-induced cells were used for determining HRI kinase activity by measuring eIF-2 alpha phosphorylation, and Hsp-HRI interaction by immunoprecipitation and immunoblot analyses. The results indicate a significant increase in both Hsp70 and Hsp90 expression during AAPH (2,2'-azobis (2-amidinopropane) dihydrochloride)-induced oxidative stress. Further, their interaction with HRI, which correlates well with its increased HRI kinase activity leads to inhibition of protein synthesis. Thus, we demonstrate that Hsps play an important role in the regulation of initiation of protein synt...
Pesticide Biochemistry and Physiology
The effect of sublethal concentrations, 0.00141% (LC20), 0.00251% (LC30), and 0.0036% (LC40) of a... more The effect of sublethal concentrations, 0.00141% (LC20), 0.00251% (LC30), and 0.0036% (LC40) of a dispersible formulation of an acylurea insect growth regulator, flufenoxuron (Cascade) on certain biochemical parameters in the larvae of Tribolium castaneum was investigated. When neonates were fed on diet treated with sublethal concentrations for 24 h, it was observed that at all concentrations tested, there was a significant reduction in chitin content on the 15th day of development. Total soluble protein content at LC20 and LC30 decreased with increasing age of the larvae. At LC20 and LC40 concentrations there was a progressive increase in the protein: chitin ratio as a function of increase in age of the larvae. SDS–PAGE analysis of the larval tissue extracts indicated gross quantitative changes in some of the protein bands (MW 50–97 kDa). Western blot analysis revealed significant increase in the level of HSP70 in the extracts of larvae fed on LC30 treated diet, on the 7th and 10th...
The international journal of biochemistry & cell biology
Erythropoiesis is controlled by a complex interplay of several signalling pathways and key transc... more Erythropoiesis is controlled by a complex interplay of several signalling pathways and key transcription factors, as well as microRNAs (miRNAs). MicroRNAs function as critical modulators of gene expression for cellular processes. In the present study, we found that miR-320a inhibits erythroid differentiation by targeting Matrix Attachment Region binding protein SMAR1. miR-320a negatively regulates the expression of SMAR1 by directly binding to its 3'UTR. In response to mild DNA damage, miR-320a expression is decreased resulting in enhanced expression of SMAR1 protein, which in turn, reduces its targets, Bax and Puma inhibiting apoptosis. Our data demonstrate that during hemin-induced erythroid differentiation, enhanced expression of SMAR1 negatively correlates with miR-320a expression. Further analysis reveals that SMAR1 regulates erythroid differentiation, by binding to the promoter of miR-221/222, which play a crucial role in early erythropoiesis. Overall, our studies provide ...
Communications in Computer and Information Science, 2010
N. Meghanathan et al. (Eds.): NeCoM, WiMoN, and WeST 2010, CCIS 90, pp. 124133, 2010. © Springer... more N. Meghanathan et al. (Eds.): NeCoM, WiMoN, and WeST 2010, CCIS 90, pp. 124133, 2010. © Springer-Verlag Berlin Heidelberg 2010 ... A Novel Visual Cryptographic Technique through Grey ... Jayanta Kumar Pal1, JK Mandal2, and Kousik Dasgupta1
2006 IEEE International Conference on Industrial Technology, 2006
Abstract The work intends to obtain the model reduction using balanced singular perturbation appr... more Abstract The work intends to obtain the model reduction using balanced singular perturbation approximation (BSPA) of any LTI system, which may be utilized for controller reduction as well. The MATLAB based computational difficulties for obtaining balanced realized minimal order model of the system necessary for preservation of DC gain of non-minimal systems have been investigated. The algorithm is illustrated by an example showing MATLAB based actual computational difficulties being faced by the researchers ...
Indian journal of biochemistry & biophysics, 1995
Antisera prepared against total water-soluble lens proteins of the shark, Scoliodon sorrakowah we... more Antisera prepared against total water-soluble lens proteins of the shark, Scoliodon sorrakowah were reacted with homologous antigen and analysed reaction products by immunoelectrophoresis (IE) and two dimensional crossed antigen-antibody electrophoresis (2D-CE). On IE, shark antigens formed 5 precipitin lines including 1 alpha, 3 beta and 1 gamma crystallins and on 2D-CE 3 alpha, 6 beta and 6 gamma peaks accounting for 8%, 27% and 65% antigen in the respective group were obtained from the total crystallins. Using anti-shark antisera, the immunocrossreactivity of lens proteins from 6 Chondropterygii, 23 teleosts and 16 higher vertebrates was examined by IE. It is found that beta crystallins are the most conserved and crossreact with all vertebrate classes, whereas gamma crystallin crossreactivity is specific to the class Pisces and alpha crystallins are least conserved and their crossreactivity is confined to subclass Chondropterygii. Based on IE patterns, a phylogenetic tree is cons...
Journal of cell science, 1988
The prosomes, a novel type of small RNA-protein complex previously characterized in avian and mam... more The prosomes, a novel type of small RNA-protein complex previously characterized in avian and mammalian cells, were isolated from axolotl (Ambystoma mexicanum) oocytes and identified by sedimentation analysis and protein composition. The prosomal nature of these particles was further ascertained by immunoblot analysis with anti-duck prosome monoclonal antibodies. By in vitro [35S]methionine labelling, de novo synthesis of prosomal proteins could be detected neither during oogenesis nor meiotic maturation. The results obtained by both indirect immunofluorescence and immunoblot analyses demonstrated a dramatic change in the localization of prosomal antigens during oocyte development. They were initially detected in the oocyte cytoplasm, during oocyte growth. At the end of vitellogenesis (stages V-VI), they entered the nucleus (germinal vesicle) and were accumulated there to the highest concentration. During oocyte maturation, after nuclear envelope breakdown, prosomal antigens were fo...
The Journal of Biochemistry, Molecular Biology and Biophysics, 2002
We have used human cell lines, namely, K562 and HeLa cells as model systems in understanding the ... more We have used human cell lines, namely, K562 and HeLa cells as model systems in understanding the mechanism of lead toxicity and heat shock, that may be mediated by the heme-regulated eIF-2alpha kinase which is also called the heme-regulated inhibitor (HRI). RT-PCR analysis using HRI-specific primers indicated a two- to three-fold increase in HRI expression in K562 and HeLa cells exposed to lead acetate and heat shock, respectively. Further, in vitro eIF-2alpha kinase assay indicated a two- to three-fold increase in HRI kinase activity during lead toxicity in K562 cells. This increase in HRI expression and its activity was accompanied by a significant decrease in cell proliferation and cell viability. This is therefore, the first report indicating that both heavy metal exposure and heat shock cause inhibition of protein synthesis not by activation of HRI alone but by its over-expression as well as activation. Our data indicate further that lead-induced inhibition of cell proliferation may be caused due to inhibition of protein synthesis resulted due to induced expression and activity of HRI.
2006 IEEE International Conference on Industrial Technology, 2006
Abstract In the paper, robust design and tuning of power system stabilizer (PSS) is considered. T... more Abstract In the paper, robust design and tuning of power system stabilizer (PSS) is considered. The objective is to tune a PSS so that the closed-loop response of a single machine-infinite bus system is made not only stable over a wider range of operating points but also the time responses are restricted to lie within specific bounds while requiring minimum control effort. The new robust PSS approach uses the Luus-Jaakola (LJ) optimization procedure in conjunction with genetic algorithm (GA). Simulation results on a ...
2008 16th International Conference on Advanced Computing and Communications, 2008
In this paper, variable block length based character / bit level transformation has been proposed... more In this paper, variable block length based character / bit level transformation has been proposed for encryption, where a block of 16 characters/128 bits has been taken into account. The block is passed through a substitution operation followed by various transpositions using multi dimensional array. The block is operated with its one-time sub key which produces intermediate output of the
Biotechnology and Applied Biochemistry, 2002
The haem-regulated eukaryotic initiation factor 2α kinase, also called the haem-regulated inhibit... more The haem-regulated eukaryotic initiation factor 2α kinase, also called the haem-regulated inhibitor (HRI), has been shown to increase in the peripheral blood cells as a function of drug-induced anaemia in rabbits, suggesting that it could be a molecular indicator of drug-induced anaemia [Anand and Pal (1997) J. Biosci. 22, 287-298]. In the present investigation, we have determined the expression of HRI during lead-induced anaemia in rabbits. The level of anaemia has been determined by routine procedures such as reticulocyte count, haemoglobin content and packed cell volume. These values were compared with the results obtained for a quantitative Western blot of HRI in the blood cell lysates of drug-and lead-induced anaemic rabbits. These results indicate that HRI could be used as a molecular marker for lead-induced anaemia since a progressive increase in HRI levels could be detected as a function of the time of lead exposure. In order to understand the role of stress proteins, heat-shock protein (Hsp) 70 and Hsp90, in inducing anaemia during lead exposure, levels of Hsp70 and Hsp90, and their interaction with HRI, have been determined. Increased levels of these proteins and their intermolecular complexes with HRI suggest their role in regulating protein synthesis during lead-induced anaemia. These observations further reiterate the use of HRI as a potential indicator for drug-and heavy-metal-induced anaemia in humans.