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Papers by Jeff Wu
Cell cycle (Georgetown, Tex.), Jan 8, 2017
Androgenetic alopecia is the most common form of hair loss. Minoxidil has been approved for the t... more Androgenetic alopecia is the most common form of hair loss. Minoxidil has been approved for the treatment of hair loss, however its mechanism of action is still not fully clarified. In this study, we aimed to elucidate the effects of 5% minoxidil topical foam on gene expression and activation of signaling pathways in vertex and frontal scalp of men with androgenetic alopecia. We identified regional variations in gene expression and perturbed signaling pathways using in silico Pathway Activation Network Decomposition Analysis (iPANDA) before and after treatment with minoxidil. Vertex and frontal scalp of patients showed a generally similar response to minoxidil. Both scalp regions showed upregulation of genes that encode keratin associated proteins and downregulation of ILK, Akt, and MAPK signaling pathways after minoxidil treatment. Our results provide new insights into the mechanism of action of minoxidil topical foam in men with androgenetic alopecia.
Journal of Investigative Dermatology, 2002
Volunteers and application method In all experiments 10 ml of each solution were applied evenly o... more Volunteers and application method In all experiments 10 ml of each solution were applied evenly on predetermined circular areas (2.9 cm diameter) on the volar forearm of each volunteer.
Applied Spectroscopy, 1994
An inexpensive peristaltic pump has been modified for computer control and coupled to a commercia... more An inexpensive peristaltic pump has been modified for computer control and coupled to a commercial autosampler to provide completely automated sample introduction and calibration via controlled-dispersion flow injection. Precision of 4.2% RSD can be achieved with an injection volume of 10 #L or larger. With this device, controlled-dispersion flow analysis was used for computer-controlled standard calibration of Ca ÷2 from a single concentrated sample; a dilution of 180-fold of a 10-#g/mL Ca ÷2 sample was readily achieved. Intelligent control of the system was also realized through the implementation of a LabVIEW software system on a Macintosh computer. Advantages of the new system are the benefit of simplicity and low cost for flow-injection analysis, a reduction in sample consumption, and the convenience of performing single-standard calibration.
Cell cycle (Georgetown, Tex.), Jan 8, 2017
Androgenetic alopecia is the most common form of hair loss. Minoxidil has been approved for the t... more Androgenetic alopecia is the most common form of hair loss. Minoxidil has been approved for the treatment of hair loss, however its mechanism of action is still not fully clarified. In this study, we aimed to elucidate the effects of 5% minoxidil topical foam on gene expression and activation of signaling pathways in vertex and frontal scalp of men with androgenetic alopecia. We identified regional variations in gene expression and perturbed signaling pathways using in silico Pathway Activation Network Decomposition Analysis (iPANDA) before and after treatment with minoxidil. Vertex and frontal scalp of patients showed a generally similar response to minoxidil. Both scalp regions showed upregulation of genes that encode keratin associated proteins and downregulation of ILK, Akt, and MAPK signaling pathways after minoxidil treatment. Our results provide new insights into the mechanism of action of minoxidil topical foam in men with androgenetic alopecia.
Journal of Investigative Dermatology, 2002
Volunteers and application method In all experiments 10 ml of each solution were applied evenly o... more Volunteers and application method In all experiments 10 ml of each solution were applied evenly on predetermined circular areas (2.9 cm diameter) on the volar forearm of each volunteer.
Applied Spectroscopy, 1994
An inexpensive peristaltic pump has been modified for computer control and coupled to a commercia... more An inexpensive peristaltic pump has been modified for computer control and coupled to a commercial autosampler to provide completely automated sample introduction and calibration via controlled-dispersion flow injection. Precision of 4.2% RSD can be achieved with an injection volume of 10 #L or larger. With this device, controlled-dispersion flow analysis was used for computer-controlled standard calibration of Ca ÷2 from a single concentrated sample; a dilution of 180-fold of a 10-#g/mL Ca ÷2 sample was readily achieved. Intelligent control of the system was also realized through the implementation of a LabVIEW software system on a Macintosh computer. Advantages of the new system are the benefit of simplicity and low cost for flow-injection analysis, a reduction in sample consumption, and the convenience of performing single-standard calibration.