Josphine Jenifer - Academia.edu (original) (raw)
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Papers by Josphine Jenifer
Brazilian Journal of Pharmaceutical Sciences
In the recent past, drug delivery through nanoparticles is considered an effective tool to treat ... more In the recent past, drug delivery through nanoparticles is considered an effective tool to treat various diseases. Biopolymeric nanoparticles such as protein based nanoparticles have vital role as drug carrier as it is non-antigenic, and easily biodegradable. Curcumin, plant polyphenolic anticancerous compound was loaded into the casein nanoparticles by coacervation method. Particle size and surface charge of spherical casein nanoparticles as observed to be 201.4 nm and-86.9 mV. The loading efficiency of curcumin loaded casein nanoparticles was found to 85.05 %. In vitro drug release was performed at different pH (7.4 and 3.0), and the cumulative release was observed to be 24.8 and 20.13% and at different temperatures (25°C and 37°C), the cumulative release was observed to be 24.8 and 28.60 % respectively in 48 h. Curcumin release from casein nanoparticles was shown to be in a steady, and prolonged rate. The nanoparticles were observed to have an effective antimocrobial activity than curcumin in free form. The drug loaded casein nanoparticles were found to be potent particles to protect cells from hydrogen peroxide and UV light damage. The cytotoxic activity of nanoparticles on MCF7 and A549 cells were assayed and was observed to have an IC50 value of 609 and 825.2µg/ml. Cell death was observed to be through apoptosis, accompanied by DNA fragmentation.
Osteochondromas are the rare benign and malignant tumour of the growing bone, usually affecting t... more Osteochondromas are the rare benign and malignant tumour of the growing bone, usually affecting the young adults. Solitary osteocartilaginous exostosis is more common than the hereditary multiple exostosis (HME). The first 3 decades of life has maximum chances of getting affected with osteochondroma and hardly occurs in craniofacial bones because of the fact that these bones are not formed by endochondral ossification. Most of the symptoms occur at the periphery of the bone tissues, with causes of osteochondroma being unknown. It involves in genetic condition and is associated with mutations of EXT1 or EXT2 genes. Diagnosis is difficult at symptomless stage, incidentally it is diagnosed when X-ray is carried out. Detection of tumor by ultra sound is accurate than other diagnosis process. No treatment is required other than regular monitoring of tumor. Standard allele specific PCR based techniques on osteochondroma showing both recombine and intact alleles were reported. The observat...
Background Nanoparticles are widely used for various therapeutic treatments. Proteins have the ad... more Background Nanoparticles are widely used for various therapeutic treatments. Proteins have the advantage of being naturally occurring, with relative lack of toxicity and antigenicity, but easy biodegradability and potent ability to bind other compounds. Proteins have been produced in nanoformulations, which have been made by various methods including desolvation and precipitation. Biopolymeric nanoparticles including proteins are used in delivery of genes and drugs, and in tissue engineering. Protein nanoparticulate drug delivery systems are used to treat several diseases because of their high drug-binding capacity, biocompatibility, nutritional value, stability, and potent site-specific action. Among the various proteins, casein and albumin nanoparticles are used widely as nanocarriers for various therapeutic drugs. Objectives To review the types of nanoparticles and their role in therapeutic applications, and the important role of casein and albumin nanoparticles in cancer therapy...
Brazilian Journal of Pharmaceutical Sciences
In the recent past, drug delivery through nanoparticles is considered an effective tool to treat ... more In the recent past, drug delivery through nanoparticles is considered an effective tool to treat various diseases. Biopolymeric nanoparticles such as protein based nanoparticles have vital role as drug carrier as it is non-antigenic, and easily biodegradable. Curcumin, plant polyphenolic anticancerous compound was loaded into the casein nanoparticles by coacervation method. Particle size and surface charge of spherical casein nanoparticles as observed to be 201.4 nm and-86.9 mV. The loading efficiency of curcumin loaded casein nanoparticles was found to 85.05 %. In vitro drug release was performed at different pH (7.4 and 3.0), and the cumulative release was observed to be 24.8 and 20.13% and at different temperatures (25°C and 37°C), the cumulative release was observed to be 24.8 and 28.60 % respectively in 48 h. Curcumin release from casein nanoparticles was shown to be in a steady, and prolonged rate. The nanoparticles were observed to have an effective antimocrobial activity than curcumin in free form. The drug loaded casein nanoparticles were found to be potent particles to protect cells from hydrogen peroxide and UV light damage. The cytotoxic activity of nanoparticles on MCF7 and A549 cells were assayed and was observed to have an IC50 value of 609 and 825.2µg/ml. Cell death was observed to be through apoptosis, accompanied by DNA fragmentation.
Osteochondromas are the rare benign and malignant tumour of the growing bone, usually affecting t... more Osteochondromas are the rare benign and malignant tumour of the growing bone, usually affecting the young adults. Solitary osteocartilaginous exostosis is more common than the hereditary multiple exostosis (HME). The first 3 decades of life has maximum chances of getting affected with osteochondroma and hardly occurs in craniofacial bones because of the fact that these bones are not formed by endochondral ossification. Most of the symptoms occur at the periphery of the bone tissues, with causes of osteochondroma being unknown. It involves in genetic condition and is associated with mutations of EXT1 or EXT2 genes. Diagnosis is difficult at symptomless stage, incidentally it is diagnosed when X-ray is carried out. Detection of tumor by ultra sound is accurate than other diagnosis process. No treatment is required other than regular monitoring of tumor. Standard allele specific PCR based techniques on osteochondroma showing both recombine and intact alleles were reported. The observat...
Background Nanoparticles are widely used for various therapeutic treatments. Proteins have the ad... more Background Nanoparticles are widely used for various therapeutic treatments. Proteins have the advantage of being naturally occurring, with relative lack of toxicity and antigenicity, but easy biodegradability and potent ability to bind other compounds. Proteins have been produced in nanoformulations, which have been made by various methods including desolvation and precipitation. Biopolymeric nanoparticles including proteins are used in delivery of genes and drugs, and in tissue engineering. Protein nanoparticulate drug delivery systems are used to treat several diseases because of their high drug-binding capacity, biocompatibility, nutritional value, stability, and potent site-specific action. Among the various proteins, casein and albumin nanoparticles are used widely as nanocarriers for various therapeutic drugs. Objectives To review the types of nanoparticles and their role in therapeutic applications, and the important role of casein and albumin nanoparticles in cancer therapy...