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Papers by Jenny Ho

Research paper thumbnail of The Role of Colloidal Delivery Systems in DNA Genetic Therapeutics

Research paper thumbnail of Delivery of DNA vaccines: an overview on the use of biodegradable polymeric and magnetic nanoparticles

Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2010

Research paper thumbnail of Preparation of stable isotope-labeled peripheral cannabinoid receptor CB2 by bacterial fermentation

Protein Expression and Purification, 2010

Research paper thumbnail of Superparamagnetic Nanoparticles for Effective Delivery of Malaria DNA Vaccine

Research paper thumbnail of The extraction of liquid, protein molecules and yeast cells from paper through surface acoustic wave atomization

Research paper thumbnail of Performance of R-N(R′)-R′′ functionalised poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolithic sorbent for plasmid DNA adsorption

Journal of Separation Science, 2007

Research paper thumbnail of Mesoporous silica spheres from colloids

Journal of Colloid and Interface Science, 2007

Research paper thumbnail of Protein loaded mesoporous silica spheres as a controlled delivery platform

Journal of Chemical Technology & Biotechnology, 2008

Research paper thumbnail of A thermal expulsion approach to homogeneous large-volume methacrylate monolith preparation; Enabling large-scale rapid purification of biomolecules

Journal of Applied Polymer Science, 2008

Research paper thumbnail of Mitigating the looming vaccine crisis: production and delivery of plasmid-based vaccines

Critical Reviews in Biotechnology, 2011

The exponentially growing human population and the emergence of new diseases are clear indication... more The exponentially growing human population and the emergence of new diseases are clear indications that the world can no longer depend solely on conventional vaccine technologies and production schemes. The race to find a new vaccine technology is crucial to help speed up and complement the World Health Organization (WHO) disease elimination program. The ultimate goal is to uncover fast and efficient production schemes in the event of a pandemic, and also to effectively fight deadly diseases such as malaria, bird flu, hepatitis, and human immunodeficiency virus (HIV). Plasmid DNA vaccines, if properly formulated, offer specific priming of the immune system and similar or even better prophylactic effects than conventional vaccines. This article discusses many of the critical issues that need to be considered when developing fast, effective, and reliable plasmid DNA vaccine manufacturing processes. Different modes of plasmid production via bacterial fermentation are compared. Plasmid purification by chromatography is specifically discussed as it is the most commercially viable bioprocess engineering technique for continuous purification of supercoiled plasmid DNA. Current techniques and progress covering the area of plasmid DNA vaccine design, formulation, and delivery are also put forward.

Research paper thumbnail of N,N′-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier

Colloids and Surfaces B: Biointerfaces, 2011

Research paper thumbnail of Using DNA as a drug—Bioprocessing and delivery strategies

Chemical Engineering Research and Design, 2009

Research paper thumbnail of Process considerations related to the microencapsulation of plasmid DNA via ultrasonic atomization

Biotechnology and Bioengineering, 2008

Research paper thumbnail of Characterization of SUMO-conjugating enzyme mutants in Schizosaccharomyces pombe identifies a dominant-negative allele that severely reduces SUMO conjugation

Biochemical Journal, 2003

Research paper thumbnail of Protein concentration in the pancreatic zymogen granule

Biochimica et Biophysica Acta (BBA) - General Subjects, 1983

We have estimated the concentration of protein in the zymogen granule, a major storage depot for ... more We have estimated the concentration of protein in the zymogen granule, a major storage depot for secretory protein in the pancreatic acinar cell, in four different ways. Each of these approaches yielded roughly similar values. The protein concentration in the granule is approx. 135-270 mg protein/ml granule volume in rat and rabbit, as compared to an average value for the protein concentration of tissue of 135 mg/g tissue for rabbits and 183 mg/g tissue for rats. This is equivalent to an average molarity for the contained proteins of between 4-9 mM based on an estimated average molecular weight for the mixture of 30 000-40 000. An upper limit for the concentration of protein in these granules can reasonably be set at about double the overall concentration of protein in the non-granule portion of the cell.

Research paper thumbnail of Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography–electrospray ionization–mass spectrometry

Analytical Biochemistry, 2008

Research paper thumbnail of Paper-based microfluidic surface acoustic wave sample delivery and ionization source for rapid and sensitive ambient mass spectrometry

Analytical chemistry, Apr 7, 2011

Research paper thumbnail of Template-free Synthesis and Encapsulation Technique for Layer-by-Layer Polymer Nanocarrier Fabrication

Research paper thumbnail of The Role of Colloidal Delivery Systems in DNA Genetic Therapeutics

Research paper thumbnail of Delivery of DNA vaccines: an overview on the use of biodegradable polymeric and magnetic nanoparticles

Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2010

Research paper thumbnail of Preparation of stable isotope-labeled peripheral cannabinoid receptor CB2 by bacterial fermentation

Protein Expression and Purification, 2010

Research paper thumbnail of Superparamagnetic Nanoparticles for Effective Delivery of Malaria DNA Vaccine

Research paper thumbnail of The extraction of liquid, protein molecules and yeast cells from paper through surface acoustic wave atomization

Research paper thumbnail of Performance of R-N(R′)-R′′ functionalised poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolithic sorbent for plasmid DNA adsorption

Journal of Separation Science, 2007

Research paper thumbnail of Mesoporous silica spheres from colloids

Journal of Colloid and Interface Science, 2007

Research paper thumbnail of Protein loaded mesoporous silica spheres as a controlled delivery platform

Journal of Chemical Technology & Biotechnology, 2008

Research paper thumbnail of A thermal expulsion approach to homogeneous large-volume methacrylate monolith preparation; Enabling large-scale rapid purification of biomolecules

Journal of Applied Polymer Science, 2008

Research paper thumbnail of Mitigating the looming vaccine crisis: production and delivery of plasmid-based vaccines

Critical Reviews in Biotechnology, 2011

The exponentially growing human population and the emergence of new diseases are clear indication... more The exponentially growing human population and the emergence of new diseases are clear indications that the world can no longer depend solely on conventional vaccine technologies and production schemes. The race to find a new vaccine technology is crucial to help speed up and complement the World Health Organization (WHO) disease elimination program. The ultimate goal is to uncover fast and efficient production schemes in the event of a pandemic, and also to effectively fight deadly diseases such as malaria, bird flu, hepatitis, and human immunodeficiency virus (HIV). Plasmid DNA vaccines, if properly formulated, offer specific priming of the immune system and similar or even better prophylactic effects than conventional vaccines. This article discusses many of the critical issues that need to be considered when developing fast, effective, and reliable plasmid DNA vaccine manufacturing processes. Different modes of plasmid production via bacterial fermentation are compared. Plasmid purification by chromatography is specifically discussed as it is the most commercially viable bioprocess engineering technique for continuous purification of supercoiled plasmid DNA. Current techniques and progress covering the area of plasmid DNA vaccine design, formulation, and delivery are also put forward.

Research paper thumbnail of N,N′-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier

Colloids and Surfaces B: Biointerfaces, 2011

Research paper thumbnail of Using DNA as a drug—Bioprocessing and delivery strategies

Chemical Engineering Research and Design, 2009

Research paper thumbnail of Process considerations related to the microencapsulation of plasmid DNA via ultrasonic atomization

Biotechnology and Bioengineering, 2008

Research paper thumbnail of Characterization of SUMO-conjugating enzyme mutants in Schizosaccharomyces pombe identifies a dominant-negative allele that severely reduces SUMO conjugation

Biochemical Journal, 2003

Research paper thumbnail of Protein concentration in the pancreatic zymogen granule

Biochimica et Biophysica Acta (BBA) - General Subjects, 1983

We have estimated the concentration of protein in the zymogen granule, a major storage depot for ... more We have estimated the concentration of protein in the zymogen granule, a major storage depot for secretory protein in the pancreatic acinar cell, in four different ways. Each of these approaches yielded roughly similar values. The protein concentration in the granule is approx. 135-270 mg protein/ml granule volume in rat and rabbit, as compared to an average value for the protein concentration of tissue of 135 mg/g tissue for rabbits and 183 mg/g tissue for rats. This is equivalent to an average molarity for the contained proteins of between 4-9 mM based on an estimated average molecular weight for the mixture of 30 000-40 000. An upper limit for the concentration of protein in these granules can reasonably be set at about double the overall concentration of protein in the non-granule portion of the cell.

Research paper thumbnail of Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography–electrospray ionization–mass spectrometry

Analytical Biochemistry, 2008

Research paper thumbnail of Paper-based microfluidic surface acoustic wave sample delivery and ionization source for rapid and sensitive ambient mass spectrometry

Analytical chemistry, Apr 7, 2011

Research paper thumbnail of Template-free Synthesis and Encapsulation Technique for Layer-by-Layer Polymer Nanocarrier Fabrication

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