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Papers by Jenny Xu
Nucleic Acids Research
Previous studies of the Drosophila melanogaster hsp26 gene promoter have demonstrated the importa... more Previous studies of the Drosophila melanogaster hsp26 gene promoter have demonstrated the importance of a homopurine´homopyrimidine segment [primarily (CT) n´( GA) n ] for chromatin structure formation and gene activation. (CT) n regions are known to bind GAGA factor, a dominant enhancer of PEV thought to play a role in generating an accessible chromatin structure. The (CT) n region can also form an H-DNA structure in vitro under acidic pH and negative supercoiling; a detailed map of that structure is reported here. To test whether the (CT) n sequence can function through H-DNA in vivo, we have analyzed a series of hsp26-lacZ transgenes with altered sequences in this region.
Nucleic Acids Research
Previous studies of the Drosophila melanogaster hsp26 gene promoter have demonstrated the importa... more Previous studies of the Drosophila melanogaster hsp26 gene promoter have demonstrated the importance of a homopurine´homopyrimidine segment [primarily (CT) n´( GA) n ] for chromatin structure formation and gene activation. (CT) n regions are known to bind GAGA factor, a dominant enhancer of PEV thought to play a role in generating an accessible chromatin structure. The (CT) n region can also form an H-DNA structure in vitro under acidic pH and negative supercoiling; a detailed map of that structure is reported here. To test whether the (CT) n sequence can function through H-DNA in vivo, we have analyzed a series of hsp26-lacZ transgenes with altered sequences in this region.