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Papers by Jesús Gil-Muñoz
Biotechnology for Biofuels, Jun 23, 2017
Background: Genomic and proteomic analysis are potent tools for metabolic characterization of mic... more Background: Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms. Although cellulose usually triggers cellulase production in cellulolytic fungi, the secretion of the different enzymes involved in polymer conversion is subjected to different factors, depending on growth conditions. These enzymes are key factors in biomass exploitation for second generation bioethanol production. Although highly effective commercial cocktails are available, they are usually deficient for β-glucosidase activity, and genera like Penicillium and Talaromyces are being explored for its production. Results: This article presents the description of Talaromyces amestolkiae as a cellulase-producer fungus that secretes high levels of β-glucosidase. β-1,4-endoglucanase, exoglucanase, and β-glucosidase activities were quantified in the presence of different carbon sources. Although the two first activities were only induced with cellulosic substrates, β-glucosidase levels were similar in all carbon sources tested. Sequencing and analysis of the genome of this fungus revealed multiple genes encoding β-glucosidases. Extracellular proteome analysis showed different induction patterns. In all conditions assayed, glycosyl hydrolases were the most abundant proteins in the supernatants, albeit the ratio of the diverse enzymes from this family depended on the carbon source. At least two different β-glucosidases have been identified in this work: one is induced by cellulose and the other one is carbon source-independent. The crudes induced by Avicel and glucose were independently used as supplements for saccharification of slurry from acid-catalyzed steam-exploded wheat straw, obtaining the highest yields of fermentable glucose using crudes induced by cellulose. Conclusions: The genome of T. amestolkiae contains several genes encoding β-glucosidases and the fungus secretes high levels of this activity, regardless of the carbon source availability, although its production is repressed by glucose. Two main different β-glucosidases have been identified from proteomic shotgun analysis. One of them is produced under different carbon sources, while the other is induced in cellulosic substrates and is a good supplement to Celluclast in saccharification of pretreated wheat straw.
Biotechnology for biofuels, 2017
Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewab... more Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewable feedstock for the production of second-generation biofuels and other chemicals. The discovery of novel, highly efficient β-glucosidases remains as one of the major bottlenecks for cellulose degradation. In this context, the ascomycete isolated from cereal samples, has been studied as a promising source for these enzymes. BGL-2 is the major β-glucosidase secreted by this fungus in the presence of cellulosic inductors. This enzyme possesses a CBD (Cellulose Binding Domain), an unusual feature among this type of proteins. Besides, when growing on cellulose, the fungus produced two different - mRNAs that were cloned and expressed in . A complete recombinant protein (BGL-2*) and its truncated form, lacking CBD (BGL-2T*), have been purified, characterized and compared with the native enzyme (BGL-2). The three BGL-2 forms studied are highly stable in a wide pH range, but BGL-2T* showed an im...
Additional file 3. Proteins in secretomes of T. amestolkiae growing with different carbon sources... more Additional file 3. Proteins in secretomes of T. amestolkiae growing with different carbon sources: 3A) Avicel; 3B) Glucose; 3C) Slurry; 3D) Xylan. The file contains four tables with the list of proteins identified in each condition.
Additional file 2. Gene prediction from T. amestolkiae genome. The file contains the amino acid s... more Additional file 2. Gene prediction from T. amestolkiae genome. The file contains the amino acid sequences of the proteins encoded by the genome of T. amestolkiae.
Additional file 1. Identification of the fungal isolate. This file contains three figures, the ma... more Additional file 1. Identification of the fungal isolate. This file contains three figures, the materials and methods associated to the information presented, and a brief discussion of the data presented. Figure S1. Maximum likelihood phylogenetic analysis of RPB1 (A), ITS (B), and BT2 (C) regions from different Talaromyces strains. Figure S2. Agar colonies of T. amestolkiae, Penicillium purpurogenum var. rubrisclerotium and Penicillium rubrum. Figure S3. SEM micrography of conidiophores and hyphae from the three fungal species.
Título: Enseñar ciencia a través de blogs de divulgación. Resumen Durante la última década hemos ... more Título: Enseñar ciencia a través de blogs de divulgación. Resumen Durante la última década hemos sido testigos de un creciente interés por las herramientas Web 2.0, con el potencial suficiente para complementar y mejorar la difusión de contenidos de cualquier índole, llevándola a una nueva dimensión. Especialmente significativo ha sido el impacto de los blogs en la comunicación de la ciencia. Han proporcionado un espacio abierto para que una audiencia diversa acceda a una información científica. La educación no es ajena, por lo que es necesario adaptarla. Se analiza el papel de los blogs y se explora algunos dedicados a la divulgación científica con contenidos trasladables al aula. Palabras clave: blogs, educación, divulgación, didáctica, ciencia.
Biotechnology for biofuels, 2017
Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms.... more Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms. Although cellulose usually triggers cellulase production in cellulolytic fungi, the secretion of the different enzymes involved in polymer conversion is subjected to different factors, depending on growth conditions. These enzymes are key factors in biomass exploitation for second generation bioethanol production. Although highly effective commercial cocktails are available, they are usually deficient for β-glucosidase activity, and genera like Penicillium and Talaromyces are being explored for its production. This article presents the description of Talaromyces amestolkiae as a cellulase-producer fungus that secretes high levels of β-glucosidase. β-1,4-endoglucanase, exoglucanase, and β-glucosidase activities were quantified in the presence of different carbon sources. Although the two first activities were only induced with cellulosic substrates, β-glucosidase levels were similar in a...
Biotechnology for biofuels, 2017
Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms.... more Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms. Although cellulose usually triggers cellulase production in cellulolytic fungi, the secretion of the different enzymes involved in polymer conversion is subjected to different factors, depending on growth conditions. These enzymes are key factors in biomass exploitation for second generation bioethanol production. Although highly effective commercial cocktails are available, they are usually deficient for β-glucosidase activity, and genera like Penicillium and Talaromyces are being explored for its production. This article presents the description of Talaromyces amestolkiae as a cellulase-producer fungus that secretes high levels of β-glucosidase. β-1,4-endoglucanase, exoglucanase, and β-glucosidase activities were quantified in the presence of different carbon sources. Although the two first activities were only induced with cellulosic substrates, β-glucosidase levels were similar in a...
Books by Jesús Gil-Muñoz
Radical Barbatilo, diez años divulgando ciencia, 2024
Biotechnology for Biofuels, Jun 23, 2017
Background: Genomic and proteomic analysis are potent tools for metabolic characterization of mic... more Background: Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms. Although cellulose usually triggers cellulase production in cellulolytic fungi, the secretion of the different enzymes involved in polymer conversion is subjected to different factors, depending on growth conditions. These enzymes are key factors in biomass exploitation for second generation bioethanol production. Although highly effective commercial cocktails are available, they are usually deficient for β-glucosidase activity, and genera like Penicillium and Talaromyces are being explored for its production. Results: This article presents the description of Talaromyces amestolkiae as a cellulase-producer fungus that secretes high levels of β-glucosidase. β-1,4-endoglucanase, exoglucanase, and β-glucosidase activities were quantified in the presence of different carbon sources. Although the two first activities were only induced with cellulosic substrates, β-glucosidase levels were similar in all carbon sources tested. Sequencing and analysis of the genome of this fungus revealed multiple genes encoding β-glucosidases. Extracellular proteome analysis showed different induction patterns. In all conditions assayed, glycosyl hydrolases were the most abundant proteins in the supernatants, albeit the ratio of the diverse enzymes from this family depended on the carbon source. At least two different β-glucosidases have been identified in this work: one is induced by cellulose and the other one is carbon source-independent. The crudes induced by Avicel and glucose were independently used as supplements for saccharification of slurry from acid-catalyzed steam-exploded wheat straw, obtaining the highest yields of fermentable glucose using crudes induced by cellulose. Conclusions: The genome of T. amestolkiae contains several genes encoding β-glucosidases and the fungus secretes high levels of this activity, regardless of the carbon source availability, although its production is repressed by glucose. Two main different β-glucosidases have been identified from proteomic shotgun analysis. One of them is produced under different carbon sources, while the other is induced in cellulosic substrates and is a good supplement to Celluclast in saccharification of pretreated wheat straw.
Biotechnology for biofuels, 2017
Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewab... more Cellulose, the most abundant biopolymer on earth, is an alternative for fossil fuels as a renewable feedstock for the production of second-generation biofuels and other chemicals. The discovery of novel, highly efficient β-glucosidases remains as one of the major bottlenecks for cellulose degradation. In this context, the ascomycete isolated from cereal samples, has been studied as a promising source for these enzymes. BGL-2 is the major β-glucosidase secreted by this fungus in the presence of cellulosic inductors. This enzyme possesses a CBD (Cellulose Binding Domain), an unusual feature among this type of proteins. Besides, when growing on cellulose, the fungus produced two different - mRNAs that were cloned and expressed in . A complete recombinant protein (BGL-2*) and its truncated form, lacking CBD (BGL-2T*), have been purified, characterized and compared with the native enzyme (BGL-2). The three BGL-2 forms studied are highly stable in a wide pH range, but BGL-2T* showed an im...
Additional file 3. Proteins in secretomes of T. amestolkiae growing with different carbon sources... more Additional file 3. Proteins in secretomes of T. amestolkiae growing with different carbon sources: 3A) Avicel; 3B) Glucose; 3C) Slurry; 3D) Xylan. The file contains four tables with the list of proteins identified in each condition.
Additional file 2. Gene prediction from T. amestolkiae genome. The file contains the amino acid s... more Additional file 2. Gene prediction from T. amestolkiae genome. The file contains the amino acid sequences of the proteins encoded by the genome of T. amestolkiae.
Additional file 1. Identification of the fungal isolate. This file contains three figures, the ma... more Additional file 1. Identification of the fungal isolate. This file contains three figures, the materials and methods associated to the information presented, and a brief discussion of the data presented. Figure S1. Maximum likelihood phylogenetic analysis of RPB1 (A), ITS (B), and BT2 (C) regions from different Talaromyces strains. Figure S2. Agar colonies of T. amestolkiae, Penicillium purpurogenum var. rubrisclerotium and Penicillium rubrum. Figure S3. SEM micrography of conidiophores and hyphae from the three fungal species.
Título: Enseñar ciencia a través de blogs de divulgación. Resumen Durante la última década hemos ... more Título: Enseñar ciencia a través de blogs de divulgación. Resumen Durante la última década hemos sido testigos de un creciente interés por las herramientas Web 2.0, con el potencial suficiente para complementar y mejorar la difusión de contenidos de cualquier índole, llevándola a una nueva dimensión. Especialmente significativo ha sido el impacto de los blogs en la comunicación de la ciencia. Han proporcionado un espacio abierto para que una audiencia diversa acceda a una información científica. La educación no es ajena, por lo que es necesario adaptarla. Se analiza el papel de los blogs y se explora algunos dedicados a la divulgación científica con contenidos trasladables al aula. Palabras clave: blogs, educación, divulgación, didáctica, ciencia.
Biotechnology for biofuels, 2017
Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms.... more Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms. Although cellulose usually triggers cellulase production in cellulolytic fungi, the secretion of the different enzymes involved in polymer conversion is subjected to different factors, depending on growth conditions. These enzymes are key factors in biomass exploitation for second generation bioethanol production. Although highly effective commercial cocktails are available, they are usually deficient for β-glucosidase activity, and genera like Penicillium and Talaromyces are being explored for its production. This article presents the description of Talaromyces amestolkiae as a cellulase-producer fungus that secretes high levels of β-glucosidase. β-1,4-endoglucanase, exoglucanase, and β-glucosidase activities were quantified in the presence of different carbon sources. Although the two first activities were only induced with cellulosic substrates, β-glucosidase levels were similar in a...
Biotechnology for biofuels, 2017
Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms.... more Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms. Although cellulose usually triggers cellulase production in cellulolytic fungi, the secretion of the different enzymes involved in polymer conversion is subjected to different factors, depending on growth conditions. These enzymes are key factors in biomass exploitation for second generation bioethanol production. Although highly effective commercial cocktails are available, they are usually deficient for β-glucosidase activity, and genera like Penicillium and Talaromyces are being explored for its production. This article presents the description of Talaromyces amestolkiae as a cellulase-producer fungus that secretes high levels of β-glucosidase. β-1,4-endoglucanase, exoglucanase, and β-glucosidase activities were quantified in the presence of different carbon sources. Although the two first activities were only induced with cellulosic substrates, β-glucosidase levels were similar in a...
Radical Barbatilo, diez años divulgando ciencia, 2024