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Papers by Jian Zhu

Research paper thumbnail of Rearranging Exosites in Noncatalytic Domains Can Redirect the Substrate Specificity of ADAMTS Proteases

Journal of Biological Chemistry, 2012

Background: ADAMTS metalloproteases are multidomain proteins with remarkable substrate specificit... more Background: ADAMTS metalloproteases are multidomain proteins with remarkable substrate specificity. Results: Swapping noncatalytic domains between ADAMTS13 and ADAMTS5 causes reciprocal changes in the cleavage of their natural substrates. Conclusion: ADAMTS exosites in noncatalytic domains are portable modifiers of proteolytic activity. Significance: Shuffling and recombination of ADAMTS ancillary structural domains may be exploited to evolve or engineer new protease functions.

Research paper thumbnail of Rearranging Exosites in Noncatalytic Domains Can Redirect the Substrate Specificity of ADAMTS Proteases

Journal of Biological Chemistry, 2012

Background: ADAMTS metalloproteases are multidomain proteins with remarkable substrate specificit... more Background: ADAMTS metalloproteases are multidomain proteins with remarkable substrate specificity. Results: Swapping noncatalytic domains between ADAMTS13 and ADAMTS5 causes reciprocal changes in the cleavage of their natural substrates. Conclusion: ADAMTS exosites in noncatalytic domains are portable modifiers of proteolytic activity. Significance: Shuffling and recombination of ADAMTS ancillary structural domains may be exploited to evolve or engineer new protease functions.

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