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Papers by Joana Chakraborty
Trends in Endocrinology & Metabolism, 1992
Experimental Cell Research, 1968
Biology of Reproduction, 1975
Trends Endocrinol Metab, 1991
Animal Reproduction Science, 1985
Four monoclonal antibodies were generated during the present investigation. Mice were immunized w... more Four monoclonal antibodies were generated during the present investigation. Mice were immunized with washed ejaculated bull spermatozoa. Spleen cells from the immunized mice were fused with myeloma cells (SP2/0) and four different hybridoma clones were obtained, producing specific monoclonal antibodies. These antibodies were designated as SP~As, SP~A2, SP1C 4 and SP1C 6 respectively. All belonged to the IgG subclaus 1. The specificity of these monoclonal antibodies was tested using both ELISA (enzyme-linked immunosorbent assay) and indirect immunofluorescence staining techniques. Quantitative estimation of antibody-antigen reaction was done by optical density measurements. Ejaculated bull spermatozoa were always reactive for each ELISA procedure. Other test cells including spleen, testicular, ovarian, uterine and pancreatic cells from both bull and rabbit were non-reactive. Bull testicular spermatozoa were also nonreactive. However, seminal fluid (without sperm) was reactive. All four monoclonal antibodies were reactive to the midpiece of the ejaculated bull spermatozoa. In addition, SP2As was also reactive to the acrosomal area and SPIA: was reactive to the acrosomal and the post-acrosomal area. Cytoplasmic droplets of ejaculated spermatozoa from bull and human were also possibly reactive to one antibody (SP~C~). The results clearly suggest the heterogeneous nature of the sperm cell plasma membrane and precise molecular alteration in the plasma membrane components (antigens) as the sperm cells differentiate and mature during their transit through the epididymis.
Urology, 1985
Torsion of the spermatic cord is not uncommon among young men. Various abnormalities in the histo... more Torsion of the spermatic cord is not uncommon among young men. Various abnormalities in the histology of the contralateral testis have been reported due to unilateral torsion of the spermatic cord. We quantitatively estimated the germ cells from three groups of men: normal ...
Journal of General Virology, 2012
Perinatal infection with a temperature-sensitive mutant (ts-1) of Moloney murine leukemia virus (... more Perinatal infection with a temperature-sensitive mutant (ts-1) of Moloney murine leukemia virus (MoMuLV) results in massive splenomegaly and thymomegaly in mice and development of lymphoma in .55 % of infected pups. Previous flow cytometry studies showed a decrease in CD4 + cells in perinatally infected pups, but cell population changes in infected animals with lymphoma compared with infected animals without lymphoma has not yet been reported. In the current study, BALB/c mice were infected with ts-1 through breast milk transmission and observed until development of clinical signs and symptoms of lymphoma and/or symptomatic ts-1 infection. Flow cytometry studies were performed on blood, spleen and thymus samples and correlated with gross morphology and histological changes, resulting from the development of lymphoma. Infected animals with lymphoma had significant decreases in CD4 + and CD8 + cell counts in blood and spleen compared with controls. The spleens of infected animals without lymphoma showed a decrease in CD4 + and CD8 + cell counts, but this was not significant compared with controls. In the thymus, CD4 + and CD8 + cell counts also decreased, but this was not significant in infected animals with and without lymphoma compared with controls. Markers of myeloid cell dysfunction increased in the thymus of animals with infection with and without lymphoma compared with controls. Thus, immunosuppression and CD4 + /CD8 + cell decreases in the spleen and thymus are associated with malignant transformation and development of lymphoma in this animal model.
Trends in Endocrinology & Metabolism, 1994
Background: ts-1 is a temperature sensitive mutant of the wild type Moloney Murine Leukemia Virus... more Background: ts-1 is a temperature sensitive mutant of the wild type Moloney Murine Leukemia Virus (Mo MuLV) which differs from wild type by 11 amino acids. Infection of susceptible mouse strains with ts-1 virus result in a well-documented syndrome of neuro-degeneration and immunodeficiency with splenic and thymic atrophy. We have developed and characterized an animal model of perinatal transmission of this retrovirus including novel observations not seen in the model of ts-1 for neurodegeneration. Methods: BALB/C mice inoculated IP 72 hours after birth with 0.1 ml of 4x106 ffu/ml ts-1 were mated at maturity. The viral transmission to fetuses and pups was evaluated. Histology and clinical features of perinatal disease were compared to those of non-perinatally acquired ts-1 infection and wild type Mo MuLV infection. Results: In utero transmission rates were as follows: 12% (2/17 fetuses) day 10 gestation, 39% (37/94 fetuses) at day 19 of gestation, 78.3% (36/46 pups) immediately after...
Background: Approximately 95% of HIV-1-associated lymphomas are considered to be of B-cell origin... more Background: Approximately 95% of HIV-1-associated lymphomas are considered to be of B-cell origin. The vast majority of these tumors are high grade B-cell lymphoma. The objective of the current study is to determine: 1) the phenotype of lymphoma associated with temperature sensitive Moloney Murine Leukemia Virus (Mo-MuLV-ts1) and 2) the alteration in mRNA expression of these lymphomas. Methods: A total of 117 BALB/c pups were divided into 4 groups: 24 (group #1) from infected mothers were allowed to suckle from their infected biological mother; 38 control pups (group #2) from non-infected mother suckled from surrogate infected mothers; 46 pups of infected mothers (group #3), suckled from control surrogate mothers and 9 control pups (group #4) suckled from their non infected biological mother. Splenic tissues were used for immunohistochemistry and qRT-PCR. Antibodies to CD3 and CD79a were used to identify the T-and B-cell lymphomas respectively. mRNA expression levels for each of 28 ...
Background: We have developed a mouse model to study the maternally inherited virus induced lymph... more Background: We have developed a mouse model to study the maternally inherited virus induced lymphoma. We have observed, 9 of 26 pups of Molony Murine Leukemia temperature sensitive virus (MoMuLv-ts-1) infected mothers developed lymphoma, while 17 of their infected littermates did not. None of the control pups of uninfected mothers developed lymphoma.Our model is unique because it is T-cell derived and similar to human AIDS related lymphomas while most murine leukemia virus are known to cause B-cell lymphoma. The goal of the present study was to examine the common integration sites (CIS) of the viral genome into the infected host genome, causing lymphoma. Methods: Genomic DNA from lymph nodes of mice with lymphoma was digested with BamH1, purified and ligated using T4 ligase for I-PCR. PCR was performed using each set of inverse primers designed from MoMuLV-ts1 sequence for the 5’end and the 3’ end products of BamH1 digest. Secondary PCR reaction was performed using primary PCR produ...
Systems Biology in Reproductive Medicine, 1983
Male rats were immobilized for 2 hr/day, 5 days a week, for varying periods of time (7-3 I days) ... more Male rats were immobilized for 2 hr/day, 5 days a week, for varying periods of time (7-3 I days) to assess the effects of stress on the reproductive system. Mice were immobilized for 25-38 days. The effectiveness of the stress was demonstrated by assessing the adrenal and body weight. Adrenal weights were significantly increased (p<.OS-t-test) compared to controls. Body weights were decreased compared to controls. No consistent change was noticed in the testicular weight and progressive motility of the spermatozoa drawn from the cauda epididymis and vas deferens. There were no deleterious effects of immobilization stress on the morphology of the differentiating germ cells, peritubular wall, and the interstitial tissues of the testis.
Reproductive BioMedicine Online, 2006
In 1990 he spent time at the University Hospital of Ghent, Belgium, where he was introduced to th... more In 1990 he spent time at the University Hospital of Ghent, Belgium, where he was introduced to the venographic approach to varicocele. In 1999 he co-developed an innovative method for the diagnosis and treatment of testicular venous incompetence.
Journal of Molecular and Cellular Cardiology, 1991
The mechanismof nlrateeffeotlvenessinpatientswlh anginapeotorls isundear. We examinedbbodflow dis... more The mechanismof nlrateeffeotlvenessinpatientswlh anginapeotorls isundear. We examinedbbodflow distribution in collateral dependent and collateral independent myooardium alter isosorbide din&ate (ISDN)
Trends in Endocrinology & Metabolism, 1992
Experimental Cell Research, 1968
Biology of Reproduction, 1975
Trends Endocrinol Metab, 1991
Animal Reproduction Science, 1985
Four monoclonal antibodies were generated during the present investigation. Mice were immunized w... more Four monoclonal antibodies were generated during the present investigation. Mice were immunized with washed ejaculated bull spermatozoa. Spleen cells from the immunized mice were fused with myeloma cells (SP2/0) and four different hybridoma clones were obtained, producing specific monoclonal antibodies. These antibodies were designated as SP~As, SP~A2, SP1C 4 and SP1C 6 respectively. All belonged to the IgG subclaus 1. The specificity of these monoclonal antibodies was tested using both ELISA (enzyme-linked immunosorbent assay) and indirect immunofluorescence staining techniques. Quantitative estimation of antibody-antigen reaction was done by optical density measurements. Ejaculated bull spermatozoa were always reactive for each ELISA procedure. Other test cells including spleen, testicular, ovarian, uterine and pancreatic cells from both bull and rabbit were non-reactive. Bull testicular spermatozoa were also nonreactive. However, seminal fluid (without sperm) was reactive. All four monoclonal antibodies were reactive to the midpiece of the ejaculated bull spermatozoa. In addition, SP2As was also reactive to the acrosomal area and SPIA: was reactive to the acrosomal and the post-acrosomal area. Cytoplasmic droplets of ejaculated spermatozoa from bull and human were also possibly reactive to one antibody (SP~C~). The results clearly suggest the heterogeneous nature of the sperm cell plasma membrane and precise molecular alteration in the plasma membrane components (antigens) as the sperm cells differentiate and mature during their transit through the epididymis.
Urology, 1985
Torsion of the spermatic cord is not uncommon among young men. Various abnormalities in the histo... more Torsion of the spermatic cord is not uncommon among young men. Various abnormalities in the histology of the contralateral testis have been reported due to unilateral torsion of the spermatic cord. We quantitatively estimated the germ cells from three groups of men: normal ...
Journal of General Virology, 2012
Perinatal infection with a temperature-sensitive mutant (ts-1) of Moloney murine leukemia virus (... more Perinatal infection with a temperature-sensitive mutant (ts-1) of Moloney murine leukemia virus (MoMuLV) results in massive splenomegaly and thymomegaly in mice and development of lymphoma in .55 % of infected pups. Previous flow cytometry studies showed a decrease in CD4 + cells in perinatally infected pups, but cell population changes in infected animals with lymphoma compared with infected animals without lymphoma has not yet been reported. In the current study, BALB/c mice were infected with ts-1 through breast milk transmission and observed until development of clinical signs and symptoms of lymphoma and/or symptomatic ts-1 infection. Flow cytometry studies were performed on blood, spleen and thymus samples and correlated with gross morphology and histological changes, resulting from the development of lymphoma. Infected animals with lymphoma had significant decreases in CD4 + and CD8 + cell counts in blood and spleen compared with controls. The spleens of infected animals without lymphoma showed a decrease in CD4 + and CD8 + cell counts, but this was not significant compared with controls. In the thymus, CD4 + and CD8 + cell counts also decreased, but this was not significant in infected animals with and without lymphoma compared with controls. Markers of myeloid cell dysfunction increased in the thymus of animals with infection with and without lymphoma compared with controls. Thus, immunosuppression and CD4 + /CD8 + cell decreases in the spleen and thymus are associated with malignant transformation and development of lymphoma in this animal model.
Trends in Endocrinology & Metabolism, 1994
Background: ts-1 is a temperature sensitive mutant of the wild type Moloney Murine Leukemia Virus... more Background: ts-1 is a temperature sensitive mutant of the wild type Moloney Murine Leukemia Virus (Mo MuLV) which differs from wild type by 11 amino acids. Infection of susceptible mouse strains with ts-1 virus result in a well-documented syndrome of neuro-degeneration and immunodeficiency with splenic and thymic atrophy. We have developed and characterized an animal model of perinatal transmission of this retrovirus including novel observations not seen in the model of ts-1 for neurodegeneration. Methods: BALB/C mice inoculated IP 72 hours after birth with 0.1 ml of 4x106 ffu/ml ts-1 were mated at maturity. The viral transmission to fetuses and pups was evaluated. Histology and clinical features of perinatal disease were compared to those of non-perinatally acquired ts-1 infection and wild type Mo MuLV infection. Results: In utero transmission rates were as follows: 12% (2/17 fetuses) day 10 gestation, 39% (37/94 fetuses) at day 19 of gestation, 78.3% (36/46 pups) immediately after...
Background: Approximately 95% of HIV-1-associated lymphomas are considered to be of B-cell origin... more Background: Approximately 95% of HIV-1-associated lymphomas are considered to be of B-cell origin. The vast majority of these tumors are high grade B-cell lymphoma. The objective of the current study is to determine: 1) the phenotype of lymphoma associated with temperature sensitive Moloney Murine Leukemia Virus (Mo-MuLV-ts1) and 2) the alteration in mRNA expression of these lymphomas. Methods: A total of 117 BALB/c pups were divided into 4 groups: 24 (group #1) from infected mothers were allowed to suckle from their infected biological mother; 38 control pups (group #2) from non-infected mother suckled from surrogate infected mothers; 46 pups of infected mothers (group #3), suckled from control surrogate mothers and 9 control pups (group #4) suckled from their non infected biological mother. Splenic tissues were used for immunohistochemistry and qRT-PCR. Antibodies to CD3 and CD79a were used to identify the T-and B-cell lymphomas respectively. mRNA expression levels for each of 28 ...
Background: We have developed a mouse model to study the maternally inherited virus induced lymph... more Background: We have developed a mouse model to study the maternally inherited virus induced lymphoma. We have observed, 9 of 26 pups of Molony Murine Leukemia temperature sensitive virus (MoMuLv-ts-1) infected mothers developed lymphoma, while 17 of their infected littermates did not. None of the control pups of uninfected mothers developed lymphoma.Our model is unique because it is T-cell derived and similar to human AIDS related lymphomas while most murine leukemia virus are known to cause B-cell lymphoma. The goal of the present study was to examine the common integration sites (CIS) of the viral genome into the infected host genome, causing lymphoma. Methods: Genomic DNA from lymph nodes of mice with lymphoma was digested with BamH1, purified and ligated using T4 ligase for I-PCR. PCR was performed using each set of inverse primers designed from MoMuLV-ts1 sequence for the 5’end and the 3’ end products of BamH1 digest. Secondary PCR reaction was performed using primary PCR produ...
Systems Biology in Reproductive Medicine, 1983
Male rats were immobilized for 2 hr/day, 5 days a week, for varying periods of time (7-3 I days) ... more Male rats were immobilized for 2 hr/day, 5 days a week, for varying periods of time (7-3 I days) to assess the effects of stress on the reproductive system. Mice were immobilized for 25-38 days. The effectiveness of the stress was demonstrated by assessing the adrenal and body weight. Adrenal weights were significantly increased (p<.OS-t-test) compared to controls. Body weights were decreased compared to controls. No consistent change was noticed in the testicular weight and progressive motility of the spermatozoa drawn from the cauda epididymis and vas deferens. There were no deleterious effects of immobilization stress on the morphology of the differentiating germ cells, peritubular wall, and the interstitial tissues of the testis.
Reproductive BioMedicine Online, 2006
In 1990 he spent time at the University Hospital of Ghent, Belgium, where he was introduced to th... more In 1990 he spent time at the University Hospital of Ghent, Belgium, where he was introduced to the venographic approach to varicocele. In 1999 he co-developed an innovative method for the diagnosis and treatment of testicular venous incompetence.
Journal of Molecular and Cellular Cardiology, 1991
The mechanismof nlrateeffeotlvenessinpatientswlh anginapeotorls isundear. We examinedbbodflow dis... more The mechanismof nlrateeffeotlvenessinpatientswlh anginapeotorls isundear. We examinedbbodflow distribution in collateral dependent and collateral independent myooardium alter isosorbide din&ate (ISDN)