Joanna Klećkowska-Nawrot - Academia.edu (original) (raw)

Papers by Joanna Klećkowska-Nawrot

Research paper thumbnail of Histological and histochemical study of the harderian gland, upper and lower eyelids and third eyelid in different age groups of the indian peafowl (Pavo cristatus)

XV Kongres Polskiego Towarzystwa Nauk Weterynaryjnych : „Per scientiam ad salutem animalium et hominum” : Materiały kongresowe, 2016

Research paper thumbnail of Characteristic features of mechanical lingual papillae in the warsaw wild captive pisula stryjek rats (WWCPS)

XV Kongres Polskiego Towarzystwa Nauk Weterynaryjnych : „Per scientiam ad salutem animalium et hominum” : Materiały kongresowe, 2016

Research paper thumbnail of Histological and ultrastructural (sem) studies of the tongue in different age groups of the indian peafowl (pavo cristatus)

XV Kongres Polskiego Towarzystwa Nauk Weterynaryjnych : „Per scientiam ad salutem animalium et hominum” : Materiały kongresowe, 2016

Research paper thumbnail of The presence of ovarian cysts in a captive Antillean manatee (Trichechus manatus manatus L. 1758)

BMC Veterinary Research, Aug 15, 2017

Background: Several pathological changes associated with reproductive systems of marine mammals h... more Background: Several pathological changes associated with reproductive systems of marine mammals have been reported in primary literature. However, no such records exist regarding ovarian cysts in the Antillean manatee (Trichechus manatus manatus L. 1758). Case presentation: A nulliparous female Antillean manatee, held in captivity at the Wroclaw Zoological Garden, died in April 2015. The animal was 370 cm long from nose to tail and weighed 670 kg. The width of manatee's fluke was 80 cm. The post-mortem examination of the reproductive system showed the numerous pathological cysts on the external surface of the left and the right ovaries. Morphologically, the cysts had varying diameters and were attached to the ovaries by stalks. Some of the cysts were thin-walled and contained fluid, while several others were solid or contained a semi-solid mass. The structure of the ovaries displayed features of the polycystic ovary syndrome (PCOS). The cysts also exhibited positivity with cytokeratin and vimentin. There were no pathological changes within the uterus, uterine tube and vagina. Conclusion: Although we were unable to definitively determine the exact source of the ovarian cysts in the studied manatee, we found that one of the causes may be age-related. Our study also revealed that ovarian cysts in the Antillean manatee form both types of corpora lutea (CL).

Research paper thumbnail of Pathological Changes in the Ovaries of an Antillean Manatee (Trichechus manatus manatus l. 1758)

Journal of Comparative Pathology, 2017

Introduction: In the ovary, the alternation in dominance between follicles and corpora lutea tran... more Introduction: In the ovary, the alternation in dominance between follicles and corpora lutea translates into a cyclical dynamic of sex hormones. The aim of this study was to understand the morphology and immunohistochemical biomarkers in this process. Material and Methods: We used 20 sections of ovaries, either in the follicular (n 5 10) or luteal (n 5 10) phase of the oestrous cycle. The phase was determined based on the morphological aspect of the ovary and uterus; the oestrogenic phase had follicular growth in the ovary and non-specific glands in the endometrium and the luteal phase had corpora lutea in the ovary and endometrial glands at their maximum coiling expression. Results: The expression of cytokeratin (CK) 7, 8/18, 14 and 20 for epithelial cells and vimentin, desmin and a-actin for stroma, vessels and muscle was performed by indirect immunohistochemistry. Blood vessels, theca cells and stroma showed positive labelling for vimentin, desmin and a-actin. Corpora lutea had strong positivity for vimentin, CK7 and 20, and weak and inconstant expression of CK8/18. Surface epithelium showed strong labelling for CK 7 and 8/18 and was negative for CK14 and 20. Rete ovarii were positive for CK8/18 and CK7. Granulosa cells and interstitial gland had moderate positivity for CK7 and 20. Conclusion: This knowledge of morphological biomarkers of the cat ovary can be used to assess other ovarian lesions.

Research paper thumbnail of Ultrastructure of the tongue in the African pygmy hedgehog ( <i>Atelerix albiventris</i> ), comparison within the family <i>Erinaceidae</i>

Acta Zoologica, Jun 26, 2021

Research paper thumbnail of The tongue of the red panda (<i>Ailurus fulgens fulgens</i> Cuvier, 1825)—a stereoscopy, light microscopy and ultrastructural analysis

PeerJ, Nov 25, 2021

In the light of recent molecular studies, there are two phylogenetic species of the red panda (Ai... more In the light of recent molecular studies, there are two phylogenetic species of the red panda (Ailurus fulgens): Ailurus fulgens fulgens and Ailurus fulgens styani. The red panda belongs to the endangered species living in the wild only in Asia and is included in the CITES list. Although the biology and diet of this species has been extensively described, the histological structure of the tongue and lingual glands has not yet been characterized in detail in relation to the lifestyle of this mammal under specific conditions and as a basis for comparative anatomical studies of the biodiversity of endemic species. Study samples were collected from two adult males of Ailurus fulgens f. held in Wrocław Zoological Garden. Both tongues were examined macroscopically; moreover, samples with lingual papillae for light microscopy and scanning electron microscopy (SEM) were collected from the apex, body and root of the tongue. Both tongues of the Ailurus fulgens f. males were approximately 9 cm long. The dorsal lingual surface was covered with mechanical and gustatory lingual papillae. Filiform papillae were observed on the apex and the body of the tongue, while small conical papillae were observed on the root of the tongue. An elongated, 1-1.5 cm long cylinder-shaped lyssa was observed in the ventral part of the apex. Moreover, most numerous and largest round in shape fungiform papillae were observed on the apex and on the border of the body and root of the tongue, located directly rostrally to 12-13 round and oval in shape vallate papillae. The SEM study showed that filiform papillae on the apex had several long secondary processes, while filiform papillae on the body of the tongue were taller and their secondary papillae were shorter than the equivalent structures on the apex of the tongue. The SEM study showed numerous taste pores on the surface of the fungiform papilla, while irregular surface of the vallate papillae, however some of them had smoother surface. Mixed glands (comprised of mucous acini and serous acini) were present within the vallum (within the connective tissue core) of the vallate papilla. Beneath the papillae more serous glands were observed, while the posterior lingual glands in the caudal part of the root of the tongue were mucoserous (mucous units were prevalent). A characteristic feature of the tongue of Ailurus How to cite this article Goździewska-Harłajczuk K, Hamouzová P, Klećkowska-Nawrot J, Čížek P. 2021. The tongue of the red panda (Ailurus fulgens fulgens Cuvier, 1825)-a stereoscopy, light microscopy and ultrastructural analysis.

Research paper thumbnail of Microstructure of the tongue surface and lingual glands of the Sulawesi bear cuscus, Ailurops ursinus (Marsupialia: Phalangeridae)—A light and scanning electron microscopic study

Acta Zoologica, Dec 3, 2020

This study aimed to evaluate the microstructure of the surface of the tongue and lingual glands o... more This study aimed to evaluate the microstructure of the surface of the tongue and lingual glands of the vulnerable native Sulawesi bear Ailurops ursinus. The study was carried out on five tongues collected from four adult animals and one young animal. There was variation in the shape of the filiform papillae. Few conical papillae were observed on the caudo-lateral surface of the tongue. The gustatory papillae included fungiform papillae on the entire lingual surface and three vallate papillae, with the largest papilla located centrally. The foliate papillae were present on all the studied tongues and consisted of parallel folia on the right and left caudo-lateral side of the tongue, which were divided into five to eight sulci. Numerous openings of the lingual glands were present on surface of the root of the tongue. Histochemical studies of the cells of the lingual glands showed the differences between young and adult animals. Thus, the composition of the secretion of these glands depends on age of animals. An aggregation of lymphatic cells was observed beneath the surface of the root epithelium. Keel (enlargement of mucosa) was present on the ventral surface of the tongue medially and was composed of the connective tissue and epithelium.

Research paper thumbnail of Microscopic structure of the tongue in the lesser hedgehog tenrec (Echinops telfairi, Afrosoricida) and its relation to phylogenesis

Anatomical Science International, Jan 13, 2020

The tongue of the lesser hedgehog tenrec (Echinops telfairi) was evaluated by light and scanning ... more The tongue of the lesser hedgehog tenrec (Echinops telfairi) was evaluated by light and scanning electron microscopy. Dorsal and lateral surfaces of the tongue were densely covered with various types of lingual papillae. Two types of mechanical papillae (filiform and conical) and two types of sensory papillae (fungiform and vallate) were observed. Rostrocaudally, the number of spikes in filiform papillae increased. Filiform, crown-like papillae were found on the radix, as well as large conical papillae with a wide base and a caudally bent single spike. Fungiform papillae were scattered in four rows on the dorsal surface. Three vallate papillae in inverse triangular layout were developed in the area of the lingual radix. Taste buds were found in the sensory papillae, i.e., fungiform and vallate. The lingual glands were predominantly seromucous. These glands were evenly scattered from the caudal portion of the lingual apex to the lingual radix. Purely serous glands were localized close to vallate papillae. A histochemical analysis of glands was performed. Results were compared to the Afrotheria (tenrec's relatives) and Eulipotyphla (animals with similar feeding habits to tenrecs). This study is the first description of the lingual morphology in the member of the Order Afrosoricida. Moreover, it compares the effect of the phylogenetic relation to the effect of the feeding habits on the lingual morphology.

Research paper thumbnail of Functional Anatomy of the Thoracic Limb of the Komodo Dragon (Varanus komodoensis)

Animals

Since the Komodo dragon has been included on The International Union for Conservation of Nature (... more Since the Komodo dragon has been included on The International Union for Conservation of Nature (IUCN) Red List of Threatened Species, it is crucial to know in detail its biology as there is a limited availability of research material on these animals—mainly those who died in zoos or whose remains were found in the wild. Anatomy is essential for understanding physiology, identification of diseases, adaptations in the environment, and behavior. In this dissection study, the relationship of individual anatomical structures was analyzed, the anatomy of the active and passive movement system of the thoracic limb was described, photographs were taken, and a radiographic examination was conducted. This species has its own differences, even within closely related lizard species. Varanus komodoensis possesses triceps muscles with three heads, and the wrist is extended with additional bones for greater flexibility of the hand. The muscles of the forelimb are analogous to the hind limb; howev...

Research paper thumbnail of Topography of cranial foramina and anaesthesia techniques of cranial nerves in selected species of primates (Cebidae, Cercopithecidae, Lemuridae) – part I – osteology

BMC Veterinary Research

Background Conductive anaesthesia of the nerves around the head is one of the methods of intraope... more Background Conductive anaesthesia of the nerves around the head is one of the methods of intraoperative pain relief (under deep anaesthesia but before proceeding with the procedure). Performing this procedure on primates is especially challenging for the veterinarian, due to their cranial anatomy and topography, which has more in common with the human skull than with the skulls of other animals. Knowledge of key bony structures, including cranial foramina, is essential for effective anaesthesia of the cranial nerves. Results In this study, the differences in the topography of the cranial foramina in eight selected species of primates were examined: Angola colobus (Colobus angolensis), Celebes crested macaque (Macaca nigra), L’Hoest’s monkey (Allochrocebus lhoesti), baboon (Papio cynocephalus), buff-bellied capuchin (Sapajus xanthosternos), black-and-white ruffed lemur (Varecia variegata), crowned lemur (Eulemur coronatus), and a ring-tailed lemur (Lemur catta) coming from the Wrocla...

Research paper thumbnail of Comparative Histology and Histochemistry of the Parotid Gland and Mandibular Gland in the Lowland Tapir (Tapirus terrestris Perissodactyla) and Aardvark (Orycteropus afer Tubulidentata)

Animals

In terrestrial mammals, the parotid and mandibular glands secrete different types of saliva into ... more In terrestrial mammals, the parotid and mandibular glands secrete different types of saliva into the oral cavity. Both glands were obtained from two female lowland tapirs (Tapirus terrestris) and one female aardvark (Orycteropus afer) from the Wroclaw Zoological Garden (Poland) and examined by light microscopy (hematoxylin and eosin, mucicarmine, periodic acid-Schiff, Alcian blue pH 1.0, Alcian blue pH 2.5, Alcian blue pH 2.5/PAS, and Hale’s dialysed iron). Both the parotid glands observed in the lowland tapir and aardvark were compound alveolar serous secretory units, and in both species, the secretion was composed of neutral and acidic mucopolysaccharides (sialo and sulfated mucins). However, in both the lowland tapir and aardvark, a histological examination found the stroma of the mandibular gland was divided into very large lobes by poorly marked connective tissue. While many interlobar and striated ducts were found in the aardvark, very few were found in the lowland tapir. The ...

Research paper thumbnail of Comparative Study of Lingual Papillae, Lingual Glands and Lyssa of the Tongue of Selected Wild Felids (Carnivora, Felidae) in Biological Aspects

Biology

Although much attention has been paid in the literature to the morphology of the tongue in variou... more Although much attention has been paid in the literature to the morphology of the tongue in various animal species, including some Felidae, it has not yet been described in detail in the vulnerable Neofelis nebulosa and Panthera leo bleyenberghi and the last concern Lynx lynx and Otocolobus manul. Therefore, the present study aimed to characterize the features of the tongue surface, lingual glands and lyssa in the above-mentioned four selected wild species of the Pantherinae and Felinae subfamilies. Macroscopic, histological, histochemical and ultrastructural analyzes were used in the present work. Comparative analyzes of the dorsal tongue surface showed the presence of mechanical lingual papillae on five subtypes of filiform papillae on the apex and body and conical papillae on the root of the tongue. Gustatory papillae in the four analyzed species were fungiform papillae and various numbers of vallate papillae. Foliate papillae were absent in P. leo bleyenberghi and L. lynx, while ...

Research paper thumbnail of Macroanatomical, Histological and Microtomographic Study of the Teeth of the Komodo Dragon (Varanus komodoensis)—Adaptation to Hunting

Biology

The present study aimed to characterize the macrostructure and microstructure of the mandibular t... more The present study aimed to characterize the macrostructure and microstructure of the mandibular teeth of the Komodo dragon (Varanus komodoensis) and the methods it uses to obtain food. Examinations were performed using a stereoscopic microscope, autofluorescence method, histological method and computed microtomography. A detailed macro- and micro-structural description of V. komodoensis mandibular teeth were made. The mandibular teeth are laterally flattened along their entire length and the dental crown is hooked caudally. The part of the nasal margin of the tooth crown is irregular, while the caudal margin of the tooth is characteristically serrated, except for the tooth base area. There are longitudinal grooves on the lingual and vestibular surfaces up to the lower third of the tooth height. The mandibular tooth is surrounded by a cuff made of the oral mucosa, containing the opening of the venom gland. In the histological structure of the tooth, the enamel covering the tooth crow...

Research paper thumbnail of Scanning electron microscopy study on the lingual and buccal papillae of the alpaca (Vicugna Pacos)

Anatomia Histologia Embryologia, 2014

Research paper thumbnail of Comparative Histology of the Cornea and Palisades of Vogt in the Different Wild Ruminants (Bovidae, Camelidae, Cervidae, Giraffidae, Tragulidae)

Animals

In the study, we data concerning the histological and morphometrical examination of the cornea an... more In the study, we data concerning the histological and morphometrical examination of the cornea and palisades of Vogt in the different species of ruminants from the families Bovidae, Camelidae, Cervidae, Giraffidae and Tragulidae, coming from the Warsaw Zoological Garden, the Wroclaw Zoological Garden and the Division of Animal Anatomy. The following ruminant species were investigated: common wildebeest, Kirk’s dik-dik, Natal red duiker, scimitar oryx, sitatunga, Philippine spotted deer, Père David’s deer, moose, reindeer, reticulated giraffe, okapi, Balabac mouse-deer and alpaca. The cornea of ruminant species such as the common wildebeest, Kirk’s dik-dik, Natal red duiker, scimitar oryx, reindeer and Balabac mouse-deer consisted of four layers (not found in the Bowman’s layer): the anterior corneal epithelium, the proper substance of the cornea, the posterior limiting membrane (Descemet’s membrane) and the posterior corneal epithelium (endothelium). The anterior corneal epithelium ...

Research paper thumbnail of Evaluations and Measurements of the Occurrence of Maxilla and Palatine Bone Asymmetry Based on 3D Printed Stereolithographic Models in Elderly Edentulous People

Applied sciences, Sep 17, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Anatomical and morphometric evaluation of the orbit, eye tunics, eyelids and orbital glands of the captive females of the South African painted dog (Lycaon pictus pictus Temminck, 1820) (Caniformia: Canidae)

PLOS ONE, 2021

In this study, we present the first data concerning the anatomical, morphometrical, histological ... more In this study, we present the first data concerning the anatomical, morphometrical, histological and histochemical study of the orbit, eye tunics, eyelids and orbital glands in South African Painted Dogs (Lycaon pictus pictus). The study was performed using eyeball morphometry, analysis of the bony orbit including its morphometry, macroscopic study, morphometry, histological examination of the eye tunics and chosen accessory organs of the eye and histochemical analysis. The orbit was funnel shaped and was open-type. There was a single ethmoid opening for the ethmoid nerve on the orbital lamina. The pupil was round, while the ciliary body occupied a relatively wide zone. The iris was brown and retina had a pigmented area. The cellulartapetum lucidumwas semi-circular and milky and was composed of 14–17 layers of tapetal cells arranged in a bricklike structure. In the lower eyelid, there was a single conjunctival lymph nodule aggregate. One or two additional large conjunctval folds wer...

Research paper thumbnail of Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study

Animals : an Open Access Journal from MDPI, 2022

Simple Summary The present study aimed to determine the possibility of limiting potential dioxin ... more Simple Summary The present study aimed to determine the possibility of limiting potential dioxin disorders of the structure of hard tissues in the offspring of intoxicated rat mothers by simultaneous administration of α-tocopherol (vitamin E) or acetylsalicylic acid (ASA). The levels of magnesium (Mg), calcium (Ca) and phosphorus (P) contained in bone tissue as indicators of the process of mineralization of hard tissues were also determined. The chaps were harvested from the offspring of eight female Buffalo rats for testing. Selected morphological, chemical and physical parameters of the teeth of the offspring of female rats from the experimental groups were analyzed. The analysis showed the effect of vitamin E and ASA for the content of Mg, Ca and P. In combination with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), vitamin E and ASA, they positively inhibit the inflammatory process, preventing the leaching of Ca and Mg from the bones. ASA counteracted this phenomenon much more effec...

Research paper thumbnail of Ziołolecznictwo w medycynie weterynaryjnej

Research paper thumbnail of Histological and histochemical study of the harderian gland, upper and lower eyelids and third eyelid in different age groups of the indian peafowl (Pavo cristatus)

XV Kongres Polskiego Towarzystwa Nauk Weterynaryjnych : „Per scientiam ad salutem animalium et hominum” : Materiały kongresowe, 2016

Research paper thumbnail of Characteristic features of mechanical lingual papillae in the warsaw wild captive pisula stryjek rats (WWCPS)

XV Kongres Polskiego Towarzystwa Nauk Weterynaryjnych : „Per scientiam ad salutem animalium et hominum” : Materiały kongresowe, 2016

Research paper thumbnail of Histological and ultrastructural (sem) studies of the tongue in different age groups of the indian peafowl (pavo cristatus)

XV Kongres Polskiego Towarzystwa Nauk Weterynaryjnych : „Per scientiam ad salutem animalium et hominum” : Materiały kongresowe, 2016

Research paper thumbnail of The presence of ovarian cysts in a captive Antillean manatee (Trichechus manatus manatus L. 1758)

BMC Veterinary Research, Aug 15, 2017

Background: Several pathological changes associated with reproductive systems of marine mammals h... more Background: Several pathological changes associated with reproductive systems of marine mammals have been reported in primary literature. However, no such records exist regarding ovarian cysts in the Antillean manatee (Trichechus manatus manatus L. 1758). Case presentation: A nulliparous female Antillean manatee, held in captivity at the Wroclaw Zoological Garden, died in April 2015. The animal was 370 cm long from nose to tail and weighed 670 kg. The width of manatee's fluke was 80 cm. The post-mortem examination of the reproductive system showed the numerous pathological cysts on the external surface of the left and the right ovaries. Morphologically, the cysts had varying diameters and were attached to the ovaries by stalks. Some of the cysts were thin-walled and contained fluid, while several others were solid or contained a semi-solid mass. The structure of the ovaries displayed features of the polycystic ovary syndrome (PCOS). The cysts also exhibited positivity with cytokeratin and vimentin. There were no pathological changes within the uterus, uterine tube and vagina. Conclusion: Although we were unable to definitively determine the exact source of the ovarian cysts in the studied manatee, we found that one of the causes may be age-related. Our study also revealed that ovarian cysts in the Antillean manatee form both types of corpora lutea (CL).

Research paper thumbnail of Pathological Changes in the Ovaries of an Antillean Manatee (Trichechus manatus manatus l. 1758)

Journal of Comparative Pathology, 2017

Introduction: In the ovary, the alternation in dominance between follicles and corpora lutea tran... more Introduction: In the ovary, the alternation in dominance between follicles and corpora lutea translates into a cyclical dynamic of sex hormones. The aim of this study was to understand the morphology and immunohistochemical biomarkers in this process. Material and Methods: We used 20 sections of ovaries, either in the follicular (n 5 10) or luteal (n 5 10) phase of the oestrous cycle. The phase was determined based on the morphological aspect of the ovary and uterus; the oestrogenic phase had follicular growth in the ovary and non-specific glands in the endometrium and the luteal phase had corpora lutea in the ovary and endometrial glands at their maximum coiling expression. Results: The expression of cytokeratin (CK) 7, 8/18, 14 and 20 for epithelial cells and vimentin, desmin and a-actin for stroma, vessels and muscle was performed by indirect immunohistochemistry. Blood vessels, theca cells and stroma showed positive labelling for vimentin, desmin and a-actin. Corpora lutea had strong positivity for vimentin, CK7 and 20, and weak and inconstant expression of CK8/18. Surface epithelium showed strong labelling for CK 7 and 8/18 and was negative for CK14 and 20. Rete ovarii were positive for CK8/18 and CK7. Granulosa cells and interstitial gland had moderate positivity for CK7 and 20. Conclusion: This knowledge of morphological biomarkers of the cat ovary can be used to assess other ovarian lesions.

Research paper thumbnail of Ultrastructure of the tongue in the African pygmy hedgehog ( <i>Atelerix albiventris</i> ), comparison within the family <i>Erinaceidae</i>

Acta Zoologica, Jun 26, 2021

Research paper thumbnail of The tongue of the red panda (<i>Ailurus fulgens fulgens</i> Cuvier, 1825)—a stereoscopy, light microscopy and ultrastructural analysis

PeerJ, Nov 25, 2021

In the light of recent molecular studies, there are two phylogenetic species of the red panda (Ai... more In the light of recent molecular studies, there are two phylogenetic species of the red panda (Ailurus fulgens): Ailurus fulgens fulgens and Ailurus fulgens styani. The red panda belongs to the endangered species living in the wild only in Asia and is included in the CITES list. Although the biology and diet of this species has been extensively described, the histological structure of the tongue and lingual glands has not yet been characterized in detail in relation to the lifestyle of this mammal under specific conditions and as a basis for comparative anatomical studies of the biodiversity of endemic species. Study samples were collected from two adult males of Ailurus fulgens f. held in Wrocław Zoological Garden. Both tongues were examined macroscopically; moreover, samples with lingual papillae for light microscopy and scanning electron microscopy (SEM) were collected from the apex, body and root of the tongue. Both tongues of the Ailurus fulgens f. males were approximately 9 cm long. The dorsal lingual surface was covered with mechanical and gustatory lingual papillae. Filiform papillae were observed on the apex and the body of the tongue, while small conical papillae were observed on the root of the tongue. An elongated, 1-1.5 cm long cylinder-shaped lyssa was observed in the ventral part of the apex. Moreover, most numerous and largest round in shape fungiform papillae were observed on the apex and on the border of the body and root of the tongue, located directly rostrally to 12-13 round and oval in shape vallate papillae. The SEM study showed that filiform papillae on the apex had several long secondary processes, while filiform papillae on the body of the tongue were taller and their secondary papillae were shorter than the equivalent structures on the apex of the tongue. The SEM study showed numerous taste pores on the surface of the fungiform papilla, while irregular surface of the vallate papillae, however some of them had smoother surface. Mixed glands (comprised of mucous acini and serous acini) were present within the vallum (within the connective tissue core) of the vallate papilla. Beneath the papillae more serous glands were observed, while the posterior lingual glands in the caudal part of the root of the tongue were mucoserous (mucous units were prevalent). A characteristic feature of the tongue of Ailurus How to cite this article Goździewska-Harłajczuk K, Hamouzová P, Klećkowska-Nawrot J, Čížek P. 2021. The tongue of the red panda (Ailurus fulgens fulgens Cuvier, 1825)-a stereoscopy, light microscopy and ultrastructural analysis.

Research paper thumbnail of Microstructure of the tongue surface and lingual glands of the Sulawesi bear cuscus, Ailurops ursinus (Marsupialia: Phalangeridae)—A light and scanning electron microscopic study

Acta Zoologica, Dec 3, 2020

This study aimed to evaluate the microstructure of the surface of the tongue and lingual glands o... more This study aimed to evaluate the microstructure of the surface of the tongue and lingual glands of the vulnerable native Sulawesi bear Ailurops ursinus. The study was carried out on five tongues collected from four adult animals and one young animal. There was variation in the shape of the filiform papillae. Few conical papillae were observed on the caudo-lateral surface of the tongue. The gustatory papillae included fungiform papillae on the entire lingual surface and three vallate papillae, with the largest papilla located centrally. The foliate papillae were present on all the studied tongues and consisted of parallel folia on the right and left caudo-lateral side of the tongue, which were divided into five to eight sulci. Numerous openings of the lingual glands were present on surface of the root of the tongue. Histochemical studies of the cells of the lingual glands showed the differences between young and adult animals. Thus, the composition of the secretion of these glands depends on age of animals. An aggregation of lymphatic cells was observed beneath the surface of the root epithelium. Keel (enlargement of mucosa) was present on the ventral surface of the tongue medially and was composed of the connective tissue and epithelium.

Research paper thumbnail of Microscopic structure of the tongue in the lesser hedgehog tenrec (Echinops telfairi, Afrosoricida) and its relation to phylogenesis

Anatomical Science International, Jan 13, 2020

The tongue of the lesser hedgehog tenrec (Echinops telfairi) was evaluated by light and scanning ... more The tongue of the lesser hedgehog tenrec (Echinops telfairi) was evaluated by light and scanning electron microscopy. Dorsal and lateral surfaces of the tongue were densely covered with various types of lingual papillae. Two types of mechanical papillae (filiform and conical) and two types of sensory papillae (fungiform and vallate) were observed. Rostrocaudally, the number of spikes in filiform papillae increased. Filiform, crown-like papillae were found on the radix, as well as large conical papillae with a wide base and a caudally bent single spike. Fungiform papillae were scattered in four rows on the dorsal surface. Three vallate papillae in inverse triangular layout were developed in the area of the lingual radix. Taste buds were found in the sensory papillae, i.e., fungiform and vallate. The lingual glands were predominantly seromucous. These glands were evenly scattered from the caudal portion of the lingual apex to the lingual radix. Purely serous glands were localized close to vallate papillae. A histochemical analysis of glands was performed. Results were compared to the Afrotheria (tenrec's relatives) and Eulipotyphla (animals with similar feeding habits to tenrecs). This study is the first description of the lingual morphology in the member of the Order Afrosoricida. Moreover, it compares the effect of the phylogenetic relation to the effect of the feeding habits on the lingual morphology.

Research paper thumbnail of Functional Anatomy of the Thoracic Limb of the Komodo Dragon (Varanus komodoensis)

Animals

Since the Komodo dragon has been included on The International Union for Conservation of Nature (... more Since the Komodo dragon has been included on The International Union for Conservation of Nature (IUCN) Red List of Threatened Species, it is crucial to know in detail its biology as there is a limited availability of research material on these animals—mainly those who died in zoos or whose remains were found in the wild. Anatomy is essential for understanding physiology, identification of diseases, adaptations in the environment, and behavior. In this dissection study, the relationship of individual anatomical structures was analyzed, the anatomy of the active and passive movement system of the thoracic limb was described, photographs were taken, and a radiographic examination was conducted. This species has its own differences, even within closely related lizard species. Varanus komodoensis possesses triceps muscles with three heads, and the wrist is extended with additional bones for greater flexibility of the hand. The muscles of the forelimb are analogous to the hind limb; howev...

Research paper thumbnail of Topography of cranial foramina and anaesthesia techniques of cranial nerves in selected species of primates (Cebidae, Cercopithecidae, Lemuridae) – part I – osteology

BMC Veterinary Research

Background Conductive anaesthesia of the nerves around the head is one of the methods of intraope... more Background Conductive anaesthesia of the nerves around the head is one of the methods of intraoperative pain relief (under deep anaesthesia but before proceeding with the procedure). Performing this procedure on primates is especially challenging for the veterinarian, due to their cranial anatomy and topography, which has more in common with the human skull than with the skulls of other animals. Knowledge of key bony structures, including cranial foramina, is essential for effective anaesthesia of the cranial nerves. Results In this study, the differences in the topography of the cranial foramina in eight selected species of primates were examined: Angola colobus (Colobus angolensis), Celebes crested macaque (Macaca nigra), L’Hoest’s monkey (Allochrocebus lhoesti), baboon (Papio cynocephalus), buff-bellied capuchin (Sapajus xanthosternos), black-and-white ruffed lemur (Varecia variegata), crowned lemur (Eulemur coronatus), and a ring-tailed lemur (Lemur catta) coming from the Wrocla...

Research paper thumbnail of Comparative Histology and Histochemistry of the Parotid Gland and Mandibular Gland in the Lowland Tapir (Tapirus terrestris Perissodactyla) and Aardvark (Orycteropus afer Tubulidentata)

Animals

In terrestrial mammals, the parotid and mandibular glands secrete different types of saliva into ... more In terrestrial mammals, the parotid and mandibular glands secrete different types of saliva into the oral cavity. Both glands were obtained from two female lowland tapirs (Tapirus terrestris) and one female aardvark (Orycteropus afer) from the Wroclaw Zoological Garden (Poland) and examined by light microscopy (hematoxylin and eosin, mucicarmine, periodic acid-Schiff, Alcian blue pH 1.0, Alcian blue pH 2.5, Alcian blue pH 2.5/PAS, and Hale’s dialysed iron). Both the parotid glands observed in the lowland tapir and aardvark were compound alveolar serous secretory units, and in both species, the secretion was composed of neutral and acidic mucopolysaccharides (sialo and sulfated mucins). However, in both the lowland tapir and aardvark, a histological examination found the stroma of the mandibular gland was divided into very large lobes by poorly marked connective tissue. While many interlobar and striated ducts were found in the aardvark, very few were found in the lowland tapir. The ...

Research paper thumbnail of Comparative Study of Lingual Papillae, Lingual Glands and Lyssa of the Tongue of Selected Wild Felids (Carnivora, Felidae) in Biological Aspects

Biology

Although much attention has been paid in the literature to the morphology of the tongue in variou... more Although much attention has been paid in the literature to the morphology of the tongue in various animal species, including some Felidae, it has not yet been described in detail in the vulnerable Neofelis nebulosa and Panthera leo bleyenberghi and the last concern Lynx lynx and Otocolobus manul. Therefore, the present study aimed to characterize the features of the tongue surface, lingual glands and lyssa in the above-mentioned four selected wild species of the Pantherinae and Felinae subfamilies. Macroscopic, histological, histochemical and ultrastructural analyzes were used in the present work. Comparative analyzes of the dorsal tongue surface showed the presence of mechanical lingual papillae on five subtypes of filiform papillae on the apex and body and conical papillae on the root of the tongue. Gustatory papillae in the four analyzed species were fungiform papillae and various numbers of vallate papillae. Foliate papillae were absent in P. leo bleyenberghi and L. lynx, while ...

Research paper thumbnail of Macroanatomical, Histological and Microtomographic Study of the Teeth of the Komodo Dragon (Varanus komodoensis)—Adaptation to Hunting

Biology

The present study aimed to characterize the macrostructure and microstructure of the mandibular t... more The present study aimed to characterize the macrostructure and microstructure of the mandibular teeth of the Komodo dragon (Varanus komodoensis) and the methods it uses to obtain food. Examinations were performed using a stereoscopic microscope, autofluorescence method, histological method and computed microtomography. A detailed macro- and micro-structural description of V. komodoensis mandibular teeth were made. The mandibular teeth are laterally flattened along their entire length and the dental crown is hooked caudally. The part of the nasal margin of the tooth crown is irregular, while the caudal margin of the tooth is characteristically serrated, except for the tooth base area. There are longitudinal grooves on the lingual and vestibular surfaces up to the lower third of the tooth height. The mandibular tooth is surrounded by a cuff made of the oral mucosa, containing the opening of the venom gland. In the histological structure of the tooth, the enamel covering the tooth crow...

Research paper thumbnail of Scanning electron microscopy study on the lingual and buccal papillae of the alpaca (Vicugna Pacos)

Anatomia Histologia Embryologia, 2014

Research paper thumbnail of Comparative Histology of the Cornea and Palisades of Vogt in the Different Wild Ruminants (Bovidae, Camelidae, Cervidae, Giraffidae, Tragulidae)

Animals

In the study, we data concerning the histological and morphometrical examination of the cornea an... more In the study, we data concerning the histological and morphometrical examination of the cornea and palisades of Vogt in the different species of ruminants from the families Bovidae, Camelidae, Cervidae, Giraffidae and Tragulidae, coming from the Warsaw Zoological Garden, the Wroclaw Zoological Garden and the Division of Animal Anatomy. The following ruminant species were investigated: common wildebeest, Kirk’s dik-dik, Natal red duiker, scimitar oryx, sitatunga, Philippine spotted deer, Père David’s deer, moose, reindeer, reticulated giraffe, okapi, Balabac mouse-deer and alpaca. The cornea of ruminant species such as the common wildebeest, Kirk’s dik-dik, Natal red duiker, scimitar oryx, reindeer and Balabac mouse-deer consisted of four layers (not found in the Bowman’s layer): the anterior corneal epithelium, the proper substance of the cornea, the posterior limiting membrane (Descemet’s membrane) and the posterior corneal epithelium (endothelium). The anterior corneal epithelium ...

Research paper thumbnail of Evaluations and Measurements of the Occurrence of Maxilla and Palatine Bone Asymmetry Based on 3D Printed Stereolithographic Models in Elderly Edentulous People

Applied sciences, Sep 17, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Anatomical and morphometric evaluation of the orbit, eye tunics, eyelids and orbital glands of the captive females of the South African painted dog (Lycaon pictus pictus Temminck, 1820) (Caniformia: Canidae)

PLOS ONE, 2021

In this study, we present the first data concerning the anatomical, morphometrical, histological ... more In this study, we present the first data concerning the anatomical, morphometrical, histological and histochemical study of the orbit, eye tunics, eyelids and orbital glands in South African Painted Dogs (Lycaon pictus pictus). The study was performed using eyeball morphometry, analysis of the bony orbit including its morphometry, macroscopic study, morphometry, histological examination of the eye tunics and chosen accessory organs of the eye and histochemical analysis. The orbit was funnel shaped and was open-type. There was a single ethmoid opening for the ethmoid nerve on the orbital lamina. The pupil was round, while the ciliary body occupied a relatively wide zone. The iris was brown and retina had a pigmented area. The cellulartapetum lucidumwas semi-circular and milky and was composed of 14–17 layers of tapetal cells arranged in a bricklike structure. In the lower eyelid, there was a single conjunctival lymph nodule aggregate. One or two additional large conjunctval folds wer...

Research paper thumbnail of Assessment of Selected Morphological, Physical and Chemical Parameters of the Teeth of the Offspring of Female Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), Taking into Account the Protective Role of Selected Antioxidants—Preliminary Study

Animals : an Open Access Journal from MDPI, 2022

Simple Summary The present study aimed to determine the possibility of limiting potential dioxin ... more Simple Summary The present study aimed to determine the possibility of limiting potential dioxin disorders of the structure of hard tissues in the offspring of intoxicated rat mothers by simultaneous administration of α-tocopherol (vitamin E) or acetylsalicylic acid (ASA). The levels of magnesium (Mg), calcium (Ca) and phosphorus (P) contained in bone tissue as indicators of the process of mineralization of hard tissues were also determined. The chaps were harvested from the offspring of eight female Buffalo rats for testing. Selected morphological, chemical and physical parameters of the teeth of the offspring of female rats from the experimental groups were analyzed. The analysis showed the effect of vitamin E and ASA for the content of Mg, Ca and P. In combination with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), vitamin E and ASA, they positively inhibit the inflammatory process, preventing the leaching of Ca and Mg from the bones. ASA counteracted this phenomenon much more effec...

Research paper thumbnail of Ziołolecznictwo w medycynie weterynaryjnej