Joaquín Duarte - Academia.edu (original) (raw)
Papers by Joaquín Duarte
Molecules, 2021
Cinnamoyl sucrose esters (CSEs) were evaluated as AGIs and their enzyme inhibition activity and p... more Cinnamoyl sucrose esters (CSEs) were evaluated as AGIs and their enzyme inhibition activity and potency were compared with gold standard acarbose. The inhibition activity of the CSEs against α-glucosidase and α-amylase depended on their structure including the number of the cinnamoyl moieties, their position, and the presence or absence of the acetyl moieties. The inhibitory values of the CSEs 2–9 generally increases in the order of mono-cinnamoyl moieties < di-cinnamoyl ≤ tri-cinnamoyl < tetra-cinnamoyl. This trend was supported from both in vitro and in silico results. Both tetra-cinnamoyl CSEs 5 and 9 showed the highest α-glucosidase inhibitory activities of 77 ± 5%, 74 ± 9%, respectively, against acarbose at 27 ± 4%, and highest α-amylase inhibitory activities of 98 ± 2%, 99 ± 1%, respectively, against acarbose at 93 ± 2%. CSEs 3, 4, 6, 7, 8 showed desired higher inhibition of α-glucosidase than α-amylase suggesting potential for further development as AGIs with reduced si...
Current Medicinal Chemistry, 2021
Introduction: Feruloyl Sucrose Esters (FSEs) are a class of Phenylpropanoid Sucrose Esters (PSEs)... more Introduction: Feruloyl Sucrose Esters (FSEs) are a class of Phenylpropanoid Sucrose Esters (PSEs) widely distributed in plants. They were investigated as potential selective Alpha Glucosidase Inhibitors (AGIs) to eliminate the side effects associated with the current commercial AGIs. The latter effectively lowers blood glucose levels in diabetic patients but causes severe gastrointestinal side effects. Methods: Systematic structure-activity relationship (SAR) studies using in silico, in vitro and in vivo experiments were used to accomplish this aim. FSEs were evaluated for their in vitro inhibition of starch and oligosaccharide digesting enzymes α-glucosidase and α-amylase followed by in silico docking studies to identify the binding modes. A lead candidate, FSE 12 was investigated in an STZ mouse model. Results: All active FSEs showed desired higher % inhibition of α-glucosidase and desired lower inhibition of α-amylase in comparison to AGI gold standard acarbose. This suggests a g...
Scientific Reports, 2019
DNA damage and DNA damage response (DDR) pathways in β-cells have received little attention espec... more DNA damage and DNA damage response (DDR) pathways in β-cells have received little attention especially in the context of type-2 diabetes. We postulate that p21 plays a key role in DDR by preventing apoptosis, associated through its overexpression triggered by DNA stand breaks (DSBs). Our results show that β-cells from chronic diabetic mice had a greater extent of DSBs as compared to their non-diabetic counterparts. Comet assays and nuclear presence of γH2AX and 53bp1 revealed increased DNA DSBs in 16 weeks old (wo) db/db β-cells as compared to age matched non-diabetic β-cells. Our study of gene expression changes in MIN6 cell line with doxorubicin (Dox) induced DNA damage, showed that the DDR was similar to primary β-cells from diabetic mice. There was significant overexpression of DDR genes, gadd45a and p21 after a 24-hr treatment. Western blot analysis revealed increased cleaved caspase3 over time, suggesting higher frequency of apoptosis due to Dox-induced DNA strand breaks. Inhi...
Revista Médico …, 2010
Materiales y métodos: estudio descriptivo, retrospectivo, longitudinal, cuyo universo son todos l... more Materiales y métodos: estudio descriptivo, retrospectivo, longitudinal, cuyo universo son todos los pacientes con diagnóstico de infección nosocom¡al atendldos en las unidades de cuidados intensivos: cardiovascular, cirugía, medicina intema y neurocirugía del hospital, en el ...
Geleneksel anlamda var olan tüketici davranışları algısı, sosyal medya reklamlarının gelişmesiyle... more Geleneksel anlamda var olan tüketici davranışları algısı, sosyal medya reklamlarının gelişmesiyle birlikte yeniden şekillenmekte ve sosyal medyada tüketici davranışı kendine özgü bir karaktere sahip olmaktadır. Sosyal medya reklamları ve tüketici davranışları ilişkisi, birçok disiplinde çeşitli araştırmalara konu olmaktadır. Instagram, en popüler sosyal medya uygulamalarından biridir ve özellikle kullanıcı profilinin büyük bir bölümünü genç kuşak oluşturmaktadır. Bu çalışmada, üniversite öğrencilerinin tüketim davranışlarında Instagram'daki sponsorlu reklamların etkileri incelenmiştir. Bu çerçevede ilk olarak, teorik kısımda; reklam, sosyal medya reklamcılığı, Instagram reklamları, tüketim ve tüketici davranışları kavramlarına açıklık getirilmeye çalışılmıştır. Bolu Abant İzzet Baysal Üniversitesi'ndeki 384 öğrenciden anket tekniği ile elde edilen veriler SPSS 20 adlı program ile analiz edilmiştir. Araştırmanın bulgularında, Instagram'daki sponsorlu reklamların, öğrencilerin tüketim davranışları üzerinde önemli etkiler oluşturduğu görülmüştür.
Molecules, 2021
Cinnamoyl sucrose esters (CSEs) were evaluated as AGIs and their enzyme inhibition activity and p... more Cinnamoyl sucrose esters (CSEs) were evaluated as AGIs and their enzyme inhibition activity and potency were compared with gold standard acarbose. The inhibition activity of the CSEs against α-glucosidase and α-amylase depended on their structure including the number of the cinnamoyl moieties, their position, and the presence or absence of the acetyl moieties. The inhibitory values of the CSEs 2–9 generally increases in the order of mono-cinnamoyl moieties < di-cinnamoyl ≤ tri-cinnamoyl < tetra-cinnamoyl. This trend was supported from both in vitro and in silico results. Both tetra-cinnamoyl CSEs 5 and 9 showed the highest α-glucosidase inhibitory activities of 77 ± 5%, 74 ± 9%, respectively, against acarbose at 27 ± 4%, and highest α-amylase inhibitory activities of 98 ± 2%, 99 ± 1%, respectively, against acarbose at 93 ± 2%. CSEs 3, 4, 6, 7, 8 showed desired higher inhibition of α-glucosidase than α-amylase suggesting potential for further development as AGIs with reduced si...
Current Medicinal Chemistry, 2021
Introduction: Feruloyl Sucrose Esters (FSEs) are a class of Phenylpropanoid Sucrose Esters (PSEs)... more Introduction: Feruloyl Sucrose Esters (FSEs) are a class of Phenylpropanoid Sucrose Esters (PSEs) widely distributed in plants. They were investigated as potential selective Alpha Glucosidase Inhibitors (AGIs) to eliminate the side effects associated with the current commercial AGIs. The latter effectively lowers blood glucose levels in diabetic patients but causes severe gastrointestinal side effects. Methods: Systematic structure-activity relationship (SAR) studies using in silico, in vitro and in vivo experiments were used to accomplish this aim. FSEs were evaluated for their in vitro inhibition of starch and oligosaccharide digesting enzymes α-glucosidase and α-amylase followed by in silico docking studies to identify the binding modes. A lead candidate, FSE 12 was investigated in an STZ mouse model. Results: All active FSEs showed desired higher % inhibition of α-glucosidase and desired lower inhibition of α-amylase in comparison to AGI gold standard acarbose. This suggests a g...
Scientific Reports, 2019
DNA damage and DNA damage response (DDR) pathways in β-cells have received little attention espec... more DNA damage and DNA damage response (DDR) pathways in β-cells have received little attention especially in the context of type-2 diabetes. We postulate that p21 plays a key role in DDR by preventing apoptosis, associated through its overexpression triggered by DNA stand breaks (DSBs). Our results show that β-cells from chronic diabetic mice had a greater extent of DSBs as compared to their non-diabetic counterparts. Comet assays and nuclear presence of γH2AX and 53bp1 revealed increased DNA DSBs in 16 weeks old (wo) db/db β-cells as compared to age matched non-diabetic β-cells. Our study of gene expression changes in MIN6 cell line with doxorubicin (Dox) induced DNA damage, showed that the DDR was similar to primary β-cells from diabetic mice. There was significant overexpression of DDR genes, gadd45a and p21 after a 24-hr treatment. Western blot analysis revealed increased cleaved caspase3 over time, suggesting higher frequency of apoptosis due to Dox-induced DNA strand breaks. Inhi...
Revista Médico …, 2010
Materiales y métodos: estudio descriptivo, retrospectivo, longitudinal, cuyo universo son todos l... more Materiales y métodos: estudio descriptivo, retrospectivo, longitudinal, cuyo universo son todos los pacientes con diagnóstico de infección nosocom¡al atendldos en las unidades de cuidados intensivos: cardiovascular, cirugía, medicina intema y neurocirugía del hospital, en el ...
Geleneksel anlamda var olan tüketici davranışları algısı, sosyal medya reklamlarının gelişmesiyle... more Geleneksel anlamda var olan tüketici davranışları algısı, sosyal medya reklamlarının gelişmesiyle birlikte yeniden şekillenmekte ve sosyal medyada tüketici davranışı kendine özgü bir karaktere sahip olmaktadır. Sosyal medya reklamları ve tüketici davranışları ilişkisi, birçok disiplinde çeşitli araştırmalara konu olmaktadır. Instagram, en popüler sosyal medya uygulamalarından biridir ve özellikle kullanıcı profilinin büyük bir bölümünü genç kuşak oluşturmaktadır. Bu çalışmada, üniversite öğrencilerinin tüketim davranışlarında Instagram'daki sponsorlu reklamların etkileri incelenmiştir. Bu çerçevede ilk olarak, teorik kısımda; reklam, sosyal medya reklamcılığı, Instagram reklamları, tüketim ve tüketici davranışları kavramlarına açıklık getirilmeye çalışılmıştır. Bolu Abant İzzet Baysal Üniversitesi'ndeki 384 öğrenciden anket tekniği ile elde edilen veriler SPSS 20 adlı program ile analiz edilmiştir. Araştırmanın bulgularında, Instagram'daki sponsorlu reklamların, öğrencilerin tüketim davranışları üzerinde önemli etkiler oluşturduğu görülmüştür.