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Papers by Joe Hubbard
Mycopathologia, 1980
Incorporation of thymidine, thymidine monophosphate (TMP), thymidine triphosphate (TTP), uridine ... more Incorporation of thymidine, thymidine monophosphate (TMP), thymidine triphosphate (TTP), uridine and orotic acid into DNA, RNA and protein in Blastomyces dermatitidis and Histoplasma capsulatum was studied utilizing a specific acid hydrolysis technique developed for these fungi. Thymidine was incorporated to the greatest extent (approximately 0.5 '),, of added label) followed by uridine, orotic acid, TMP and TTP. In Blastomyces, uridine and orotic acid labeled primarily RNA. TMP and TTP labeled RNA, DNA and protein at nearly the same level. In Histoplasma RNA was labeled poorly by any of these precursors. TMP and TTP labeled DNA predominately and protein to a slightly lower level. Deoxyadenosine or uridine media supplements of 250 pg/ml did not enhance incorporation. All precursors tested were found to be nonspecific in that RNA, DNA and protein were labeled. All data indicate that neither RNA nor DNA synthesis can be specifically measured in whole cells or acid precipitates by any of these precursors. Specific radiometric monitoring with these isotopes tberefore requires the separation of these macromolecules.
Depositional sequence mapping is a powerful technique to assist basin evaluation on a passive con... more Depositional sequence mapping is a powerful technique to assist basin evaluation on a passive continental margin. The study method described in this paper helps to establish the tectonic and stratigraphic framework and provides a rapid assessment of hydrocarbon potential. The technique produces more realistic stratigraphic predictions in areas of sparse well control than can be achieved with isolated studies of geophysical structural mapping and well log lithostratigraphic correlation. The basis of the study method is to use an integrated geological/geophysical database to define the sedimentary record in terms of depositional sequences. Sequences are separated by widespread unconformity surfaces and form the fundamental stratigraphic building blocks of basin analysis. In effect, each depositional sequence is a single basin entity possessing a basin margin, a depocenter and an internal facies pattern. Sequences can be stacked together into groups of related sequences, a megasequence...
Mycopathologia, 1980
Incorporation of thymidine, thymidine monophosphate (TMP), thymidine triphosphate (TTP), uridine ... more Incorporation of thymidine, thymidine monophosphate (TMP), thymidine triphosphate (TTP), uridine and orotic acid into DNA, RNA and protein in Blastomyces dermatitidis and Histoplasma capsulatum was studied utilizing a specific acid hydrolysis technique developed for these fungi. Thymidine was incorporated to the greatest extent (approximately 0.5 '),, of added label) followed by uridine, orotic acid, TMP and TTP. In Blastomyces, uridine and orotic acid labeled primarily RNA. TMP and TTP labeled RNA, DNA and protein at nearly the same level. In Histoplasma RNA was labeled poorly by any of these precursors. TMP and TTP labeled DNA predominately and protein to a slightly lower level. Deoxyadenosine or uridine media supplements of 250 pg/ml did not enhance incorporation. All precursors tested were found to be nonspecific in that RNA, DNA and protein were labeled. All data indicate that neither RNA nor DNA synthesis can be specifically measured in whole cells or acid precipitates by any of these precursors. Specific radiometric monitoring with these isotopes tberefore requires the separation of these macromolecules.
Depositional sequence mapping is a powerful technique to assist basin evaluation on a passive con... more Depositional sequence mapping is a powerful technique to assist basin evaluation on a passive continental margin. The study method described in this paper helps to establish the tectonic and stratigraphic framework and provides a rapid assessment of hydrocarbon potential. The technique produces more realistic stratigraphic predictions in areas of sparse well control than can be achieved with isolated studies of geophysical structural mapping and well log lithostratigraphic correlation. The basis of the study method is to use an integrated geological/geophysical database to define the sedimentary record in terms of depositional sequences. Sequences are separated by widespread unconformity surfaces and form the fundamental stratigraphic building blocks of basin analysis. In effect, each depositional sequence is a single basin entity possessing a basin margin, a depocenter and an internal facies pattern. Sequences can be stacked together into groups of related sequences, a megasequence...