John Pollinger - Academia.edu (original) (raw)
Papers by John Pollinger
Journal of the European Ceramic Society, 2002
A commercial Si3N4-based aqueous slurry (AS800) was processed to enable the forming of shapes via... more A commercial Si3N4-based aqueous slurry (AS800) was processed to enable the forming of shapes via a new method called colloidal isopressing. The as-received slurry was reformulated at pH 9–10 to contain 2 wt.% PEG-silane (N-[(triethoxysilyl)propyl]-O-polyethylene oxide urethane) and 0.5 M of either tetramethylammonium chloride or tetramethylammonium nitrate. The addition of the salt to an AS800 slurry formulated in the dispersed
Journal of the American Ceramic Society, 2004
ABSTRACT
Journal of the American Ceramic Society, 1994
Journal of Materials Research, 1988
Journal of Veterinary Behavior: Clinical Applications and Research, Volume 4, Issue 2, Pages 71, ... more Journal of Veterinary Behavior: Clinical Applications and Research, Volume 4, Issue 2, Pages 71, March 2009, Authors:Robert Wayne; John Pollinger; Klaus Koepfli; Carolyne Bardeleben;Melissa Gray; Carlos Bustamante; Bridgett vonHoldt; Nate Sutter; Elaine Ostrander. ...
Evolutionary Applications, 2014
Current biology : CB, Jan 8, 2014
Large carnivores can be particularly sensitive to the effects of habitat fragmentation on genetic... more Large carnivores can be particularly sensitive to the effects of habitat fragmentation on genetic diversity [1, 2]. The Santa Monica Mountains (SMMs), a large natural area within Greater Los Angeles, is completely isolated by urban development and the 101 freeway to the north. Yet the SMMs support a population of mountain lions (Puma concolor), a very rare example of a large carnivore persisting within the boundaries of a megacity. GPS locations of radio-collared lions indicate that freeways are a near-absolute barrier to movement. We genotyped 42 lions using 54 microsatellite loci and found that genetic diversity in SMM lions, prior to 2009, was lower than that for any population in North America except in southern Florida, where inbreeding depression led to reproductive failure [3-5]. We document multiple instances of father-daughter inbreeding and high levels of intraspecific strife, including the unexpected behavior of a male killing two of his offspring and a mate and his son k...
PLoS genetics, 2014
The Tibetan grey wolf (Canis lupus chanco) occupies habitats on the Qinghai-Tibet Plateau, a high... more The Tibetan grey wolf (Canis lupus chanco) occupies habitats on the Qinghai-Tibet Plateau, a high altitude (>3000 m) environment where low oxygen tension exerts unique selection pressure on individuals to adapt to hypoxic conditions. To identify genes involved in hypoxia adaptation, we generated complete genome sequences of nine Chinese wolves from high and low altitude populations at an average coverage of 25× coverage. We found that, beginning about 55,000 years ago, the highland Tibetan grey wolf suffered a more substantial population decline than lowland wolves. Positively selected hypoxia-related genes in highland wolves are enriched in the HIF signaling pathway (P = 1.57E-6), ATP binding (P = 5.62E-5), and response to an oxygen-containing compound (P≤5.30E-4). Of these positively selected hypoxia-related genes, three genes (EPAS1, ANGPT1, and RYR2) had at least one specific fixed non-synonymous SNP in highland wolves based on the nine genome data. Our re-sequencing studies ...
Veterinary Microbiology, 2012
Molecular Phylogenetics and Evolution, 2007
Molecular Ecology Resources, 2009
We characterized 15 polymorphic tetranucleotide microsatellite markers for the ringtail, Bassaris... more We characterized 15 polymorphic tetranucleotide microsatellite markers for the ringtail, Bassariscus astutus. We tested these loci in 21 individuals captured in Arizona and Texas and found six to 19 alleles per locus. Observed and expected heterozygosities ranged from 0.381 to 1.000 and from 0.381 to 0.941, respectively. All loci were in Hardy-Weinberg equilibrium, and none were in linkage disequilibrium. These markers may be used to investigate population genetics and mating patterns in this species.
Journal of Virological Methods, 2011
Efficient influenza A viral surveillance of wild and domestic birds requires rapid viral detectio... more Efficient influenza A viral surveillance of wild and domestic birds requires rapid viral detection and quantitation of high and low quality samples. Current influenza A qPCR-based detection protocols specified by CDC, OIE and USDA utilize fluorogenic hydrolysis probe based real-time reverse transcription PCR (RRT-PCR) assays for detection and quantitation. The sequence diversity of this virus, even in the conserved matrix gene M1, makes primer and probe designs challenging. In this report it was determined that false RRT-PCR positives are possible with this method. This is particularly problematic when surveying non-cultured or inactivated avian tracheal and cloacal mucosal samples with low concentrations of virus and large proportions of background nucleic acids. This report presents a modification of a one-step RRT-PCR detection method for influenza A using SYBR green intercalating dye-based target amplification detection. High Resolution Melting (HRM), amplicon size quantitation and sequence verification is used to screen for non-target amplification (false positives). The resulting protocol has the sensitivity of hydrolysis probe methods, allows for flexible primer design and verification of target amplification, and provides high confidence in positive results. A multiplex subtype detection method using the RRT-PCR HRM assay is also demonstrated. Overall, this method is both time and cost effective while providing an extra measure of confidence in surveillance results through the implementation of target verification.
Journal of the European Ceramic Society, 2002
A commercial Si3N4-based aqueous slurry (AS800) was processed to enable the forming of shapes via... more A commercial Si3N4-based aqueous slurry (AS800) was processed to enable the forming of shapes via a new method called colloidal isopressing. The as-received slurry was reformulated at pH 9–10 to contain 2 wt.% PEG-silane (N-[(triethoxysilyl)propyl]-O-polyethylene oxide urethane) and 0.5 M of either tetramethylammonium chloride or tetramethylammonium nitrate. The addition of the salt to an AS800 slurry formulated in the dispersed
Journal of the American Ceramic Society, 2004
ABSTRACT
Journal of the American Ceramic Society, 1994
Journal of Materials Research, 1988
Journal of Veterinary Behavior: Clinical Applications and Research, Volume 4, Issue 2, Pages 71, ... more Journal of Veterinary Behavior: Clinical Applications and Research, Volume 4, Issue 2, Pages 71, March 2009, Authors:Robert Wayne; John Pollinger; Klaus Koepfli; Carolyne Bardeleben;Melissa Gray; Carlos Bustamante; Bridgett vonHoldt; Nate Sutter; Elaine Ostrander. ...
Evolutionary Applications, 2014
Current biology : CB, Jan 8, 2014
Large carnivores can be particularly sensitive to the effects of habitat fragmentation on genetic... more Large carnivores can be particularly sensitive to the effects of habitat fragmentation on genetic diversity [1, 2]. The Santa Monica Mountains (SMMs), a large natural area within Greater Los Angeles, is completely isolated by urban development and the 101 freeway to the north. Yet the SMMs support a population of mountain lions (Puma concolor), a very rare example of a large carnivore persisting within the boundaries of a megacity. GPS locations of radio-collared lions indicate that freeways are a near-absolute barrier to movement. We genotyped 42 lions using 54 microsatellite loci and found that genetic diversity in SMM lions, prior to 2009, was lower than that for any population in North America except in southern Florida, where inbreeding depression led to reproductive failure [3-5]. We document multiple instances of father-daughter inbreeding and high levels of intraspecific strife, including the unexpected behavior of a male killing two of his offspring and a mate and his son k...
PLoS genetics, 2014
The Tibetan grey wolf (Canis lupus chanco) occupies habitats on the Qinghai-Tibet Plateau, a high... more The Tibetan grey wolf (Canis lupus chanco) occupies habitats on the Qinghai-Tibet Plateau, a high altitude (>3000 m) environment where low oxygen tension exerts unique selection pressure on individuals to adapt to hypoxic conditions. To identify genes involved in hypoxia adaptation, we generated complete genome sequences of nine Chinese wolves from high and low altitude populations at an average coverage of 25× coverage. We found that, beginning about 55,000 years ago, the highland Tibetan grey wolf suffered a more substantial population decline than lowland wolves. Positively selected hypoxia-related genes in highland wolves are enriched in the HIF signaling pathway (P = 1.57E-6), ATP binding (P = 5.62E-5), and response to an oxygen-containing compound (P≤5.30E-4). Of these positively selected hypoxia-related genes, three genes (EPAS1, ANGPT1, and RYR2) had at least one specific fixed non-synonymous SNP in highland wolves based on the nine genome data. Our re-sequencing studies ...
Veterinary Microbiology, 2012
Molecular Phylogenetics and Evolution, 2007
Molecular Ecology Resources, 2009
We characterized 15 polymorphic tetranucleotide microsatellite markers for the ringtail, Bassaris... more We characterized 15 polymorphic tetranucleotide microsatellite markers for the ringtail, Bassariscus astutus. We tested these loci in 21 individuals captured in Arizona and Texas and found six to 19 alleles per locus. Observed and expected heterozygosities ranged from 0.381 to 1.000 and from 0.381 to 0.941, respectively. All loci were in Hardy-Weinberg equilibrium, and none were in linkage disequilibrium. These markers may be used to investigate population genetics and mating patterns in this species.
Journal of Virological Methods, 2011
Efficient influenza A viral surveillance of wild and domestic birds requires rapid viral detectio... more Efficient influenza A viral surveillance of wild and domestic birds requires rapid viral detection and quantitation of high and low quality samples. Current influenza A qPCR-based detection protocols specified by CDC, OIE and USDA utilize fluorogenic hydrolysis probe based real-time reverse transcription PCR (RRT-PCR) assays for detection and quantitation. The sequence diversity of this virus, even in the conserved matrix gene M1, makes primer and probe designs challenging. In this report it was determined that false RRT-PCR positives are possible with this method. This is particularly problematic when surveying non-cultured or inactivated avian tracheal and cloacal mucosal samples with low concentrations of virus and large proportions of background nucleic acids. This report presents a modification of a one-step RRT-PCR detection method for influenza A using SYBR green intercalating dye-based target amplification detection. High Resolution Melting (HRM), amplicon size quantitation and sequence verification is used to screen for non-target amplification (false positives). The resulting protocol has the sensitivity of hydrolysis probe methods, allows for flexible primer design and verification of target amplification, and provides high confidence in positive results. A multiplex subtype detection method using the RRT-PCR HRM assay is also demonstrated. Overall, this method is both time and cost effective while providing an extra measure of confidence in surveillance results through the implementation of target verification.