John Stenos - Academia.edu (original) (raw)

Papers by John Stenos

Research paper thumbnail of Scrub typhus reinfection

Tropical Doctor, Nov 14, 2017

Research paper thumbnail of Isolation of Coxiella burnetii from serum of patients with acute Q fever

Journal of Microbiological Methods, Dec 1, 2015

Research paper thumbnail of The rickettsial outer-membrane protein A and B genes of Rickettsia australis, the most divergent rickettsia of the spotted fever group

International Journal of Systematic and Evolutionary Microbiology, Sep 1, 2000

Research paper thumbnail of Tick‐borne infectious diseases in Australia

The Medical Journal of Australia, Apr 1, 2017

Research paper thumbnail of Spotted fever group rickettsial infection in South-Eastern Australia: Isolation of rickettsiae

Comparative Immunology Microbiology and Infectious Diseases, Jul 1, 1993

Research paper thumbnail of Comparison of Conventional, Nested, and Real-Time Quantitative PCR for Diagnosis of Scrub Typhus

Journal of Clinical Microbiology, Feb 1, 2011

Research paper thumbnail of Aponomma Hydrosauri, the Reptile-Associated Tick Reservoir of Rickettsia Honei on Flinders Island, Australia

American Journal of Tropical Medicine and Hygiene, Sep 1, 2003

Research paper thumbnail of A Concise Review of the Epidemiology and Diagnostics of Rickettsioses: Rickettsia and Orientia spp

Journal of Clinical Microbiology, Aug 1, 2018

Research paper thumbnail of Rickettsia honei sp. nov., the aetiological agent of Flinders Island spotted fever in Australia

International journal of systematic bacteriology, Oct 1, 1998

Research paper thumbnail of Rickettsia felis: molecular characterization of a new member of the spotted fever group

International Journal of Systematic and Evolutionary Microbiology, Mar 1, 2001

Research paper thumbnail of A Short Report on the Lack of a Pyrogenic Response of Australian Genomic Group IV Isolates of Coxiella burnetii in Guinea Pigs

Tropical Medicine and Infectious Disease, Jan 25, 2019

Research paper thumbnail of Domestic dogs are mammalian reservoirs for the emerging zoonosis flea-borne spotted fever, caused by Rickettsia felis

Scientific Reports, Mar 5, 2020

Research paper thumbnail of Rapid detection and molecular characterization of australian human rickettsial isolates

Research paper thumbnail of Sequence analysis of travel-related SARS-CoV-2 cases in the Greater Geelong region, Australia

medRxiv (Cold Spring Harbor Laboratory), Jun 9, 2020

Research paper thumbnail of Selection of Diagnostic Cutoffs for Murine Typhus IgM and IgG Immunofluorescence Assay: A Systematic Review

American Journal of Tropical Medicine and Hygiene, Jul 8, 2020

Research paper thumbnail of One Health approach to controlling a Q fever outbreak on an Australian goat farm

Epidemiology and Infection, Oct 23, 2015

Research paper thumbnail of Q fever seroprevalence in Australia suggests one in twenty people have been exposed

Epidemiology and Infection, 2020

Q fever (caused by Coxiella burnetii) is thought to have an almost worldwide distribution, but fe... more Q fever (caused by Coxiella burnetii) is thought to have an almost worldwide distribution, but few countries have conducted national serosurveys. We measured Q fever seroprevalence using residual sera from diagnostic laboratories across Australia. Individuals aged 1-79 years in 2012-2013 were sampled to be proportional to the population distribution by region, distance from metropolitan areas and gender. A 1/50 serum dilution was tested for the Phase II IgG antibody against C. burnetii by indirect immunofluorescence. We calculated crude seroprevalence estimates by age group and gender, as well as age standardised national and metropolitan/non-metropolitan seroprevalence estimates. Of 2785 sera, 99 tested positive. Age standardised seroprevalence was 5.6% (95% confidence interval (CI 4.5%-6.8%), and similar in metropolitan (5.5%; 95% CI 4.1%-6.9%) and non-metropolitan regions (6.0%; 95%CI 4.0%-8.0%). More males were seropositive (6.9%; 95% CI 5.2%-8.6%) than females (4.2%; 95% CI 2.9%-5.5%) with peak seroprevalence at 50-59 years (9.2%; 95% CI 5.2%-13.3%). Q fever seroprevalence for Australia was higher than expected (especially in metropolitan regions) and higher than estimates from the Netherlands (2.4%; pre-outbreak) and US (3.1%), but lower than for Northern Ireland (12.8%). Robust country-specific seroprevalence estimates, with detailed exposure data, are required to better understand who is at risk and the need for preventive measures.

Research paper thumbnail of <i>Rickettsia felis</i>in Fleas, Western Australia

Emerging Infectious Diseases, May 1, 2006

Research paper thumbnail of Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment

Diagnostic Microbiology and Infectious Disease, 2013

Research paper thumbnail of Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing

Pathogens

Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. Ho... more Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of C. burnetii in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive C. burnetii samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the IS1111 gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The in-silico MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST C. burnetii genotypes among Iranian d...

Research paper thumbnail of Scrub typhus reinfection

Tropical Doctor, Nov 14, 2017

Research paper thumbnail of Isolation of Coxiella burnetii from serum of patients with acute Q fever

Journal of Microbiological Methods, Dec 1, 2015

Research paper thumbnail of The rickettsial outer-membrane protein A and B genes of Rickettsia australis, the most divergent rickettsia of the spotted fever group

International Journal of Systematic and Evolutionary Microbiology, Sep 1, 2000

Research paper thumbnail of Tick‐borne infectious diseases in Australia

The Medical Journal of Australia, Apr 1, 2017

Research paper thumbnail of Spotted fever group rickettsial infection in South-Eastern Australia: Isolation of rickettsiae

Comparative Immunology Microbiology and Infectious Diseases, Jul 1, 1993

Research paper thumbnail of Comparison of Conventional, Nested, and Real-Time Quantitative PCR for Diagnosis of Scrub Typhus

Journal of Clinical Microbiology, Feb 1, 2011

Research paper thumbnail of Aponomma Hydrosauri, the Reptile-Associated Tick Reservoir of Rickettsia Honei on Flinders Island, Australia

American Journal of Tropical Medicine and Hygiene, Sep 1, 2003

Research paper thumbnail of A Concise Review of the Epidemiology and Diagnostics of Rickettsioses: Rickettsia and Orientia spp

Journal of Clinical Microbiology, Aug 1, 2018

Research paper thumbnail of Rickettsia honei sp. nov., the aetiological agent of Flinders Island spotted fever in Australia

International journal of systematic bacteriology, Oct 1, 1998

Research paper thumbnail of Rickettsia felis: molecular characterization of a new member of the spotted fever group

International Journal of Systematic and Evolutionary Microbiology, Mar 1, 2001

Research paper thumbnail of A Short Report on the Lack of a Pyrogenic Response of Australian Genomic Group IV Isolates of Coxiella burnetii in Guinea Pigs

Tropical Medicine and Infectious Disease, Jan 25, 2019

Research paper thumbnail of Domestic dogs are mammalian reservoirs for the emerging zoonosis flea-borne spotted fever, caused by Rickettsia felis

Scientific Reports, Mar 5, 2020

Research paper thumbnail of Rapid detection and molecular characterization of australian human rickettsial isolates

Research paper thumbnail of Sequence analysis of travel-related SARS-CoV-2 cases in the Greater Geelong region, Australia

medRxiv (Cold Spring Harbor Laboratory), Jun 9, 2020

Research paper thumbnail of Selection of Diagnostic Cutoffs for Murine Typhus IgM and IgG Immunofluorescence Assay: A Systematic Review

American Journal of Tropical Medicine and Hygiene, Jul 8, 2020

Research paper thumbnail of One Health approach to controlling a Q fever outbreak on an Australian goat farm

Epidemiology and Infection, Oct 23, 2015

Research paper thumbnail of Q fever seroprevalence in Australia suggests one in twenty people have been exposed

Epidemiology and Infection, 2020

Q fever (caused by Coxiella burnetii) is thought to have an almost worldwide distribution, but fe... more Q fever (caused by Coxiella burnetii) is thought to have an almost worldwide distribution, but few countries have conducted national serosurveys. We measured Q fever seroprevalence using residual sera from diagnostic laboratories across Australia. Individuals aged 1-79 years in 2012-2013 were sampled to be proportional to the population distribution by region, distance from metropolitan areas and gender. A 1/50 serum dilution was tested for the Phase II IgG antibody against C. burnetii by indirect immunofluorescence. We calculated crude seroprevalence estimates by age group and gender, as well as age standardised national and metropolitan/non-metropolitan seroprevalence estimates. Of 2785 sera, 99 tested positive. Age standardised seroprevalence was 5.6% (95% confidence interval (CI 4.5%-6.8%), and similar in metropolitan (5.5%; 95% CI 4.1%-6.9%) and non-metropolitan regions (6.0%; 95%CI 4.0%-8.0%). More males were seropositive (6.9%; 95% CI 5.2%-8.6%) than females (4.2%; 95% CI 2.9%-5.5%) with peak seroprevalence at 50-59 years (9.2%; 95% CI 5.2%-13.3%). Q fever seroprevalence for Australia was higher than expected (especially in metropolitan regions) and higher than estimates from the Netherlands (2.4%; pre-outbreak) and US (3.1%), but lower than for Northern Ireland (12.8%). Robust country-specific seroprevalence estimates, with detailed exposure data, are required to better understand who is at risk and the need for preventive measures.

Research paper thumbnail of <i>Rickettsia felis</i>in Fleas, Western Australia

Emerging Infectious Diseases, May 1, 2006

Research paper thumbnail of Comparison of the performance of IFA, CFA, and ELISA assays for the serodiagnosis of acute Q fever by quality assessment

Diagnostic Microbiology and Infectious Disease, 2013

Research paper thumbnail of Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing

Pathogens

Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. Ho... more Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of C. burnetii in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive C. burnetii samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the IS1111 gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The in-silico MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST C. burnetii genotypes among Iranian d...