John T. Dulaney - Academia.edu (original) (raw)
Papers by John T. Dulaney
Journal of Japan Oil Chemists' Society, 1999
Journal of Japan Oil Chemists' Society, 1996
Journal of Oleo Science, 2001
滋賀大学教育学部紀要 自然科学 教育科学, Mar 15, 1996
日本油化学会誌 Journal of Japan Oil Chemists Society, Feb 20, 2000
Journal of Oleo Science, 2002
Glycosphingolipids, which consist of sugar and ceramide moieties, are amphipathic molecules prese... more Glycosphingolipids, which consist of sugar and ceramide moieties, are amphipathic molecules present in the plasma membrane of eucaryotic cells, where they are involved in a variety of functions, including cell-cell recognition and interaction, differentiation, oncogene-sis, immunity and so on (5,6). Many structural variations have been reported in glycosphingolipids from the Mammalia and/or other vertebrate species (7,8). We have been interested in the structural relationship between the vertebrate and invertebrate glycosphingolipids and have so far found numerous unique glycosphingolipids in invertebrate species, especially from
Journal of Japan Oil Chemists' Society, 1998
Journal of Japan Oil Chemists' Society, 1997
Journal of Oleo Science, 2002
Methods in Enzymology, 1974
Journal of Japan Oil Chemists' Society, 2000
The acidic glycolipid fraction from whole tissues of the lugworm, Tylorrhynchus heterochetus that... more The acidic glycolipid fraction from whole tissues of the lugworm, Tylorrhynchus heterochetus that inhabits brackish water was resolved into more than seven components each showing Man (ƒ¿1-2) Ins (1•¨)-P-Cer for AGL4 and Man (ƒ¿1-2) [Fuc (ƒ¿1-5)] Ins (1•¨)-P-Cer for AGL5, respectively. Aliphatic constituents of both lipids were primarily palmitic and stearic acids, and C18-sphingoids, octadecasphinganine and octadeca-4-sphingenine.
Journal of Japan Oil Chemists' Society, 2000
Advances in experimental medicine and biology, 1976
Biochimica et biophysica acta, Jan 7, 1995
The novel amphoteric glycosphingolipids containing a choline phosphate were purified from whole t... more The novel amphoteric glycosphingolipids containing a choline phosphate were purified from whole tissues of the earthworm, Pheretima hilgendorfi. Their chemical structures were completely characterized as cholinephosphoryl-->6(Man alpha 1-4)Gal beta 1-6Gal beta 1-1Cer (cholinephosphorylmannosylneogalabiaosylceramide, named PGL3a) and cholinephosphoryl-->6Gal beta 1-6Gal beta 1-6Gal beta 1-1Cer (cholinephosphorylneogalatriaosylceramide, named PGL3b) by compositional sugar, fatty acid and sphingoid analyses, hydrogen fluoride degradation, partial acid hydrolysis, methylation analysis, exoglycosidase degradation, proton magnetic resonance spectroscopy and fast atom bombardment mass spectrometry. The ceramide moieties of these lipids consisted of 22:0, 23:0 and 24:0 acids as major fatty acids, and branched octadeca- and nonadeca-4-sphingenines and octadeca-4-sphingenine as main sphingoids. Since the oligosaccharides and the ceramide moieties of PGL3a and PGL3b were identical with t...
Biochimica et biophysica acta, Jan 12, 1983
A monounsaturated and a triunsaturated form of phytanic acid (3,7,11,15-tetramethylhexacosanoate)... more A monounsaturated and a triunsaturated form of phytanic acid (3,7,11,15-tetramethylhexacosanoate) were isolated from plasma lipids of a patient with Refsum disease. Both were converted to their methyl esters, oxidized to polyhydroxy acids by treatment with OsO4 and converted to their vicinal trimethylsilyl ethers. These derivatives were analyzed by gas chromatography-mass spectrometry using both electron impact ionization (at 21 and 70 eV) and chemical ionization conditions to obtain clear evidence to establish the structure of the monounsaturated form of phytanic acid as 3,7,11,15-tetramethylhexadec-15-monoenoic acid and that of the triunsaturated form of phytanic acid as 3,7,11,15-tetramethylhexadec-6,10,14-trienoic acid. The possible metabolic and dietary sources for these novel fatty acids are discussed.
Journal of lipid research, 1974
The livers of four patients with metachromatic leukodystrophy contained galactosyl sulfatide and ... more The livers of four patients with metachromatic leukodystrophy contained galactosyl sulfatide and lactosyl sulfatide, whereas these substances were undetectable in normal human liver. On the basis of methanolysis and permethylation studies, both sulfatides were shown to be substituted with sulfate at the C-3 position of the galactose moiety. Examination of the fatty acid compositions of these sulfatides showed that C(22:0) and higher 2-hydroxy and nonhydroxy fatty acids predominated in both. Both sulfatides contained the same long-chain bases, predominantly sphingosine, dihydrosphingosine, and phytosphingosine. Using as criteria the proportion of lactosyl sulfatide to galactosyl sulfatide, and the fatty acid and long-chain base compositions, the liver sulfatides from subjects with metachromatic leukodystrophy closely resemble those in the kidney and differ from those in brain and peripheral nerve.
Journal of lipid research, 1974
Conditions have been determined for the benzoylation of ceramides containing nonhydroxy and hydro... more Conditions have been determined for the benzoylation of ceramides containing nonhydroxy and hydroxy fatty acids, and a high performance liquid chromatography system for the separation and measurement of these derivatives has been devised that is capable of good resolution and high sensitivity. These methods have been used to determine quantitatively the levels of ceramides in human tissues, and in serum and urine, and to demonstrate elevated amounts of ceramide in Farber's disease urine and tissues.
The Journal of biological chemistry, Jan 10, 1972
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1978
Two novel branched-chain fatty acids, which appear to be unsaturated analogs of phytanic acid, ha... more Two novel branched-chain fatty acids, which appear to be unsaturated analogs of phytanic acid, have been observed in sera and urine of patients with Refsum's disease. They occur in both phospholipids and neutral lipids, and have been isolated and characterized.
Journal of Japan Oil Chemists' Society, 1996
Journal of Japan Oil Chemists' Society, 1999
Journal of Japan Oil Chemists' Society, 1996
Journal of Oleo Science, 2001
滋賀大学教育学部紀要 自然科学 教育科学, Mar 15, 1996
日本油化学会誌 Journal of Japan Oil Chemists Society, Feb 20, 2000
Journal of Oleo Science, 2002
Glycosphingolipids, which consist of sugar and ceramide moieties, are amphipathic molecules prese... more Glycosphingolipids, which consist of sugar and ceramide moieties, are amphipathic molecules present in the plasma membrane of eucaryotic cells, where they are involved in a variety of functions, including cell-cell recognition and interaction, differentiation, oncogene-sis, immunity and so on (5,6). Many structural variations have been reported in glycosphingolipids from the Mammalia and/or other vertebrate species (7,8). We have been interested in the structural relationship between the vertebrate and invertebrate glycosphingolipids and have so far found numerous unique glycosphingolipids in invertebrate species, especially from
Journal of Japan Oil Chemists' Society, 1998
Journal of Japan Oil Chemists' Society, 1997
Journal of Oleo Science, 2002
Methods in Enzymology, 1974
Journal of Japan Oil Chemists' Society, 2000
The acidic glycolipid fraction from whole tissues of the lugworm, Tylorrhynchus heterochetus that... more The acidic glycolipid fraction from whole tissues of the lugworm, Tylorrhynchus heterochetus that inhabits brackish water was resolved into more than seven components each showing Man (ƒ¿1-2) Ins (1•¨)-P-Cer for AGL4 and Man (ƒ¿1-2) [Fuc (ƒ¿1-5)] Ins (1•¨)-P-Cer for AGL5, respectively. Aliphatic constituents of both lipids were primarily palmitic and stearic acids, and C18-sphingoids, octadecasphinganine and octadeca-4-sphingenine.
Journal of Japan Oil Chemists' Society, 2000
Advances in experimental medicine and biology, 1976
Biochimica et biophysica acta, Jan 7, 1995
The novel amphoteric glycosphingolipids containing a choline phosphate were purified from whole t... more The novel amphoteric glycosphingolipids containing a choline phosphate were purified from whole tissues of the earthworm, Pheretima hilgendorfi. Their chemical structures were completely characterized as cholinephosphoryl-->6(Man alpha 1-4)Gal beta 1-6Gal beta 1-1Cer (cholinephosphorylmannosylneogalabiaosylceramide, named PGL3a) and cholinephosphoryl-->6Gal beta 1-6Gal beta 1-6Gal beta 1-1Cer (cholinephosphorylneogalatriaosylceramide, named PGL3b) by compositional sugar, fatty acid and sphingoid analyses, hydrogen fluoride degradation, partial acid hydrolysis, methylation analysis, exoglycosidase degradation, proton magnetic resonance spectroscopy and fast atom bombardment mass spectrometry. The ceramide moieties of these lipids consisted of 22:0, 23:0 and 24:0 acids as major fatty acids, and branched octadeca- and nonadeca-4-sphingenines and octadeca-4-sphingenine as main sphingoids. Since the oligosaccharides and the ceramide moieties of PGL3a and PGL3b were identical with t...
Biochimica et biophysica acta, Jan 12, 1983
A monounsaturated and a triunsaturated form of phytanic acid (3,7,11,15-tetramethylhexacosanoate)... more A monounsaturated and a triunsaturated form of phytanic acid (3,7,11,15-tetramethylhexacosanoate) were isolated from plasma lipids of a patient with Refsum disease. Both were converted to their methyl esters, oxidized to polyhydroxy acids by treatment with OsO4 and converted to their vicinal trimethylsilyl ethers. These derivatives were analyzed by gas chromatography-mass spectrometry using both electron impact ionization (at 21 and 70 eV) and chemical ionization conditions to obtain clear evidence to establish the structure of the monounsaturated form of phytanic acid as 3,7,11,15-tetramethylhexadec-15-monoenoic acid and that of the triunsaturated form of phytanic acid as 3,7,11,15-tetramethylhexadec-6,10,14-trienoic acid. The possible metabolic and dietary sources for these novel fatty acids are discussed.
Journal of lipid research, 1974
The livers of four patients with metachromatic leukodystrophy contained galactosyl sulfatide and ... more The livers of four patients with metachromatic leukodystrophy contained galactosyl sulfatide and lactosyl sulfatide, whereas these substances were undetectable in normal human liver. On the basis of methanolysis and permethylation studies, both sulfatides were shown to be substituted with sulfate at the C-3 position of the galactose moiety. Examination of the fatty acid compositions of these sulfatides showed that C(22:0) and higher 2-hydroxy and nonhydroxy fatty acids predominated in both. Both sulfatides contained the same long-chain bases, predominantly sphingosine, dihydrosphingosine, and phytosphingosine. Using as criteria the proportion of lactosyl sulfatide to galactosyl sulfatide, and the fatty acid and long-chain base compositions, the liver sulfatides from subjects with metachromatic leukodystrophy closely resemble those in the kidney and differ from those in brain and peripheral nerve.
Journal of lipid research, 1974
Conditions have been determined for the benzoylation of ceramides containing nonhydroxy and hydro... more Conditions have been determined for the benzoylation of ceramides containing nonhydroxy and hydroxy fatty acids, and a high performance liquid chromatography system for the separation and measurement of these derivatives has been devised that is capable of good resolution and high sensitivity. These methods have been used to determine quantitatively the levels of ceramides in human tissues, and in serum and urine, and to demonstrate elevated amounts of ceramide in Farber's disease urine and tissues.
The Journal of biological chemistry, Jan 10, 1972
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1978
Two novel branched-chain fatty acids, which appear to be unsaturated analogs of phytanic acid, ha... more Two novel branched-chain fatty acids, which appear to be unsaturated analogs of phytanic acid, have been observed in sera and urine of patients with Refsum's disease. They occur in both phospholipids and neutral lipids, and have been isolated and characterized.
Journal of Japan Oil Chemists' Society, 1996