John Tsitsipis - Academia.edu (original) (raw)

Papers by John Tsitsipis

Aphids in a new …, 2004

Aphids are the insect group most sensitive to climate change because of their very short generati... more Aphids are the insect group most sensitive to climate change because of their very short generation times and rapid potential growth rates. A network of suction traps, 12.2 m tall, has been established in 19 countries to monitor these insects. The combined system ...

Journal of Applied Entomology, 2006

ABSTRACT Three products allowed in organic farming (kaolin, mineral oil and insecticidal soap) ... more ABSTRACT Three products allowed in organic farming (kaolin, mineral oil and insecticidal soap) as alternatives to chemical insecticides, were evaluated for the control of Myzus persicae (Sulzer) in a peach orchard. Their efficacy was compared with the neonicotinoid imidacloprid and an untreated control. The experiment was replicated for 2 years (2003 and 2004). The products were applied once in May every year and aphid infestation was recorded three to four times post-treatment. In 2004, the effect of the products on the predatory coccinellid species was also recorded. In 2003, all four products showed good control with average Abbott's efficiency ranging from 80% to 88%. In the next year, however, the products were less effective and only imidacloprid and kaolin reduced aphid population significantly compared with the untreated control, with an average Abbott's efficiency of 62% and 31%, respectively. This reduced efficiency was attributed to high rainfall occurring post-treatment. Regarding the effects on beneficial coccinellids, only imidacloprid and mineral oil reduced their numbers after application. The results showed that the three alternative products and especially kaolin are promising for aphid control in peach orchards. Kaolin has also low side-effects on beneficials and it could be used in organic peach orchards.

Bulletin of Entomological Research, 2002

During the years 1995–1999 the life cycle category of 2797 clones of Myzus persicae (Sulzer) was ... more During the years 1995–1999 the life cycle category of 2797 clones of Myzus persicae (Sulzer) was examined. The clones originated from primary and secondary hosts from different localities of North and Central Greece and the island of Crete in the south. Four different overwintering life cycle strategies were found that have also been described for M. persicae and other heteroecious species previously. A geographical variation was found in the proportion of holocyclic clones from tobacco and other secondary hosts associated with the abundance of the primary host in the sampling regions. In Central Macedonia, around the main peach-growing regions, the proportion of holocyclic clones was mostly above 50% and in some cases reached 100%. In localities of East Macedonia, holocyclic clones were also frequent. On the other hand, further south or in north-eastern Greece, where peach is not common, the proportion of holocyclic clones varied between 0 and 33%. Fifty seven percent of examined a...

Acta zoologica …, 2006

Pheromone traps, Pherocon 1C, baited with pheromone caps for Theresimima ampellophaga were used t... more Pheromone traps, Pherocon 1C, baited with pheromone caps for Theresimima ampellophaga were used to detect this species at 16 sites in Greece during 2001-2004. Catches of T. ampellophaga were recorded only at 4 of the 16 sites investigated - two of them in Central Greece ...

Journal of Applied Entomology, Nov 1, 2003

The development and the use of an information system (Centaur) including aphid trapping data from... more The development and the use of an information system (Centaur) including aphid trapping data from suction and yellow traps are described. Centaur is a client-server database on an NT server and the interface is in Windows. The system allows users to enter and retrieve data of aphid captures and to produce summaries of data at various time levels (daily, weekly, yearly) for specific sites. The data can be illustrated graphically and they can be exported or printed. In addition, the system allows the incorporation of biological data and morphological images, and maps which estimate the spatial distribution of aphids. The information retrieval is fast and user-friendly. The system can be of considerable value for research, community ecology, plant protection, public and private authorities, plant protection specialists, agronomists, agrochemical companies, farmers and the agricultural industry as it can be used for decision support. The data can be used to make forecasts for risk and/or to give advice for timely interventions. The system is currently operated in Greece and includes data from 1993 but it can be applied to other European countries and can be used for other insect pests.

Aphid Migration and Forecasting Euraphid Systems in European Community Countries, 1987

Environmental Entomology, Jan 31, 1998

Environ. Entomol. 27(1): 53-58 (1998) ABSTRACT The influence of photoperiod and temperature on th... more Environ. Entomol. 27(1): 53-58 (1998) ABSTRACT The influence of photoperiod and temperature on the termination of diapause of Sesamia l1onagrioides (Levebvre) was evaluated under laboratory and field conditions. Long-day photoperiod enhanced termination of diapause. Larvae ...

J Appl Entomol, 2009

Hautung vorhandenen inneren Druck standhalten noch den an der HIutung beteiligten Muskein Halt ge... more Hautung vorhandenen inneren Druck standhalten noch den an der HIutung beteiligten Muskein Halt gebcn. Daraus resultiert eine Unfahigkeit zum Abwerfen der Haut und schliei3lich der Tod. Dadurch, dal3 Dimilin die parasitischen und rauberischen Insekten schont, und ungiftig fur Warmbluter ist, erhalt es groi3e Bedeutung fur die kunftige umweltschoncnde Schadlingsbekimpfung.

Several papers have been published on aphid fauna in Greece during the last two decades, but the ... more Several papers have been published on aphid fauna in Greece during the last two decades, but the number of recorded species is still low compared to other European countries, including some from the Mediterranean basin. In this context, we collected aphids from various hostplants and regions in southern, central and northern Greece characterized by diverse flora, climatic conditions and ecological habitats. In total, 128 aphid species belonging to 55 genera and six subfamilies were collected on 200 host-species. Most of the species dominated the subfamily Aphidinae (especially tribes Macrosiphini and Aphidini). Among the species collected, 18 were new records in Greece. The present work improves our knowledge regarding the aphid fauna of Greece and suggests that the number of recorded species could increase further if additional studies were undertaken.

Several papers have been published on aphid fauna in Greece during the last two decades, but the ... more Several papers have been published on aphid fauna in Greece during the last two decades, but the number of recorded species is still low compared to other European countries, including some from the Mediterranean basin. In this context, we collected aphids from various host-plants and regions in southern, central and northern Greece characterized by diverse flora, cli-matic conditions and ecological habitats. In total, 128 aphid species belonging to 55 genera and six subfamilies were collected on 200 host-species. Most of the species dominated the subfami-ly Aphidinae (especially tribes Macrosiphini and Aphidini). Among the species collected, 18 were new records in Greece. The present work improves our knowledge regarding the aphid fauna of Greece and suggests that the number of recorded species could increase further if ad-ditional studies were undertaken.

Zeitschrift für Angewandte Entomologie, 1977

Biological Journal of the Linnean Society, 2014

We present data on the frequency of amplified E4 and FE4 carboxylesterase genes in Myzus persicae... more We present data on the frequency of amplified E4 and FE4 carboxylesterase genes in Myzus persicae s.l. clones collected during the years 2002-2007 and 2012 in Greece. Most clones were of the tobacco aphid, Myzus persicae nicotianae. Samples from 2012 were genotyped with microsatellite DNA markers and a number of them were karyotyped. Aphid clones with amplified FE4 genes predominated in all years, whereas E4 was present in only 3.5% of all samples and always occurred in clones with FE4. Most of the clones examined showed high carboxylesterase activity levels (R2 resistant category). The results showed marked changes in the frequencies of the two carboxylesterase genes in the tobacco aphid populations compared to published data that were collected in Greece in the mid 1990s, when E4 was recorded on its own in 20% of all samples and in 32% of samples from tobacco. A parallel change in karyotype was also observed because the A1,3 translocation, which had a worldwide association with amplified E4 genes in the 1990s, was not detected in the clones analyzed in 2012. Possible causes for these changes are discussed, although selection as a result of pest management practices appears to be the major one. Novel chromosomal rearrangements were also found in M. persicae nicotianae clones. These rearrangements could be a result of clastogenic effects of nicotine, which could persist because of the holocentric nature of aphid chromosomes. The results are discussed in relation to rapid evolution events that have taken place in the tobacco aphid in Greece during the last two decades.

Zeitschrift für Angewandte Entomologie, 1982

For 4 consecutive years population studies were made on the olive fruit fly in the Sebronas valle... more For 4 consecutive years population studies were made on the olive fruit fly in the Sebronas valley which is located at ca 600 m altitude in Western Crete. Olive flies were trapped in olive trees at highest numbers in A ril May and August-October. Fruit infestation was very intense in spring and fall on old fruits Eftinharvested and new fruits, respectively. Relatively high numbers of flies were also trapped in walnut, cherry, a ple and plane trees, in order of preference, and very few in chestnut, arbutus and vine. Very few lies were trap ed in the above plants after September, that is in the eriod olive flies oviposit intensively in the oive fruits. At the beginning of summer, most females [ad no develo ed eggs in the ovaries and no sperm was detected in their spermathecae. In the few females that hafeggs, the number of eggs per female was much smaller than in spring or fall. During this period the weather is warm-dry and no olive fruits, old unharvested or new ones, suitable for oviposition exist in the trees. In the 4th year of this when no insecticides were applied against the olive fly in the valley due to small crop ;r!%ction, extensive larval mortality was observed in fall due to parasitization.

Zeitschrift für Angewandte Entomologie, 1977

The development of an efficient caging and egging system was achieved. Firstly, an optimal ovipos... more The development of an efficient caging and egging system was achieved. Firstly, an optimal oviposition substrate in a cone shape was found. Between four nylon gauzes of different density (mesh), and four waxes for coating the gauzes (paraffin of m. p. 46-48' C, paraffin m. p. 52-54' C, a mixture of the two plus bee's wax, and ceresin), the best combination was made by the second coarser gauze coated in ceresin. The highest number of eggs were deposited in it, and the shortest oviposition duration was observed. Parafilm was inferior to oviposition cones and domes, and cones were superior to domes only in large cages, housing four large cones. Secondly, a density of 1800-2400 insects per large cage was found to be the optimal one for mass rearing. White ceresin cones were better than dark green or bladr ones.

Journal of Insect Physiology, 1970

Modifications are described in the formulation of an artificial diet for rearing Myms persicae. U... more Modifications are described in the formulation of an artificial diet for rearing Myms persicae. Using this aphid species and the dietary improvements, we determined the relative growth of larvae, derived from ascorbic acid-deprived apterous mothers, on diets containing ascorbic acid or related compounds at various concentrations. L-Dehydroascorbic acid (the oxidized form of L-ascorbic acid) is utilizable, but only about 50 per cent as efficiently as equivalent amounts of L-ascorbic acid. D-Araboascorbic acid (an epimer of L-ascorbic acid about C,) is almost equivalent to L-ascorbic acid. D-Glucuronoy-lactone and L-gulono-y-lactone (intermediates in the biosynthesis of ascorbic acid in some mammals) are not utilized to any extent, nor was reduced glutathione (an unrelated reducing substance). The results are discussed in relation to the enzyme specificity and biosynthetic capabilities of other insects and animals.

Journal of Applied Entomology, 1996

Entomologia Experimentalis et Applicata, 1977

The effect of various concentrations of agar and cellulose on the efficiency of larval diets for ... more The effect of various concentrations of agar and cellulose on the efficiency of larval diets for Dacus oleae (Gmel.) was investigated. Agar ranged from 0-2g and cellulose from O-3Og/55ml H,O. Puparial recovery varied from 546.5% of hatched eggs; in diets with 0.258, and higher than 1.5g agar, it declined at the highest and lowest cellulose levels; in those with 0.5-1.25 g agar it was high at all cellulose levels except the poor 0.5:30; diet 0:30 gave the highest yield (86.5%). Larval development was IO-15 days (50% pupariation) and it was longest at very low or high levels of both materials except 0~30 in which it was short. Growth (3.2-6.9mg) was lower in diets at the highest levels of both materials except 0:30 in which it was high. Adult emergence (75-98%) was not correlated with puparial weight or recovery. From diet 0130, diet T was derived by taking out peanuts and omitting heating without any loss in the efficiency. The advantages and improved physical characteristics of the agarless media with the increased cellulose levels are discussed.

Entomologia Experimentalis et Applicata, 1980

The effect of various relative humidities on the eggs of artificially reared olive fruit flies, D... more The effect of various relative humidities on the eggs of artificially reared olive fruit flies, Dacus oleae, was examined at 20 ~ At constant humidities no eggs hatched below 90% r.h., while egg hatch at 100% and 95% r.h. was 96.7% and 72%, respectively. Reduced hatchability was observed when eggs were exposed to non-saturated atmospheres either at the beginning or at intermediate stages of embryonic development. The lower the humidity and the longer the exposure, the lower was the egg hatch. The early stages of development were more sensitive to non-saturated humidities than stages older than 24 hr. Egg development was extended at lower humidities and longer exposures. Effect of humidity on olive fruit fly eggs is compared with other insects, and discussed in relation to egg handling in the mass rearing of the fly.

Aphids in a new …, 2004

Journal of Applied Entomology, 2006

Bulletin of Entomological Research, 2002

Acta zoologica …, 2006

Journal of Applied Entomology, Nov 1, 2003

Aphid Migration and Forecasting Euraphid Systems in European Community Countries, 1987

Environmental Entomology, Jan 31, 1998

J Appl Entomol, 2009

Several papers have been published on aphid fauna in Greece during the last two decades, but the ... more Several papers have been published on aphid fauna in Greece during the last two decades, but the number of recorded species is still low compared to other European countries, including some from the Mediterranean basin. In this context, we collected aphids from various host-plants and regions in southern, central and northern Greece characterized by diverse flora, cli-matic conditions and ecological habitats. In total, 128 aphid species belonging to 55 genera and six subfamilies were collected on 200 host-species. Most of the species dominated the subfami-ly Aphidinae (especially tribes Macrosiphini and Aphidini). Among the species collected, 18 were new records in Greece. The present work improves our knowledge regarding the aphid fauna of Greece and suggests that the number of recorded species could increase further if ad-ditional studies were undertaken.

Zeitschrift für Angewandte Entomologie, 1977

Biological Journal of the Linnean Society, 2014

Zeitschrift für Angewandte Entomologie, 1982

Zeitschrift für Angewandte Entomologie, 1977

Journal of Insect Physiology, 1970

Journal of Applied Entomology, 1996

Entomologia Experimentalis et Applicata, 1977

Entomologia Experimentalis et Applicata, 1980