Jong-Eun Won - Academia.edu (original) (raw)

Papers by Jong-Eun Won

Experimental Biology and Medicine, 2019

Injection laryngoplasty is a widely used therapeutic option for drug delivery into vocal folds (V... more Injection laryngoplasty is a widely used therapeutic option for drug delivery into vocal folds (VFs). Efficient injectable materials are urgently needed for treating intractable VF disease. Liquid-type non-thermal atmospheric plasma (LTP) has been found to be useful for various biological applications, including in regenerative medicine. We evaluated the effects of LTP on VF regeneration. Migration and matrix metalloproteinase-2 expression of lipopolysaccharide (LPS)-treated human vocal fold-derived mesenchymal stem cells (VF-MSCs) were enhanced by LTP treatment. LTP treatment not only ameliorated nuclear factor-κB and interleukin-6 activation, induced by LPS treatment, but also the increased manifestation of α-smooth muscle actin and fibronectin, induced by transforming growth factor-ß. In a rabbit VF scarring animal model, histological analyses showed increased hyaluronic acid deposition and decreased collagen accumulation after LTP injection. Videokymographic analysis showed more...

Macromolecular Bioscience

Science Advances, 2021

Integration of a smart contact lens and a heat patch realizes personal management of ocular surfa... more Integration of a smart contact lens and a heat patch realizes personal management of ocular surface diseases wirelessly.

Tissue Engineering and Regenerative Medicine, 2017

Although many graft materials have been used for augmentation rhinoplasty, an ideal graft has not... more Although many graft materials have been used for augmentation rhinoplasty, an ideal graft has not yet been developed. As the field of tissue engineering has been developing, it has been applied to the reconstruction of many organs, but its application in the rhinoplasty field is still limited. This study evaluated the utility of allogenic chondrocytes with fibrin/hyaluronic acid (HA)-poly(Llactic-co-glycolic acid) (PLGA) constructs in augmentation rhinoplasty. Chondrocytes from rabbit auricular cartilage were isolated and cultured with fibrin/HA hydrogels and implanted into PLGA scaffolds. After 8 weeks of in vitro culture, the scaffolds were implanted in the nasal dorsum of six rabbits. Eight weeks postoperatively, the implanted sites were evaluated with gross, radiologic, and histologic analysis. In vitro, more than 90% of the seeded chondrocytes in the PLGA scaffolds survived for 2 weeks, and they produced a large amount of extracellular matrix and were well differentiated. The grafts maintained their initial shape for 8 weeks after implantation. Radiological and histological evaluations showed that the structure was well maintained with minimal inflammatory response and appropriate elevation levels. However, the formation of neo-chondrocytes was not observed. PLGA scaffolds seeded with fibrin/HA and allogenic chondrocytes can be a biocompatible augmentation material in rhinoplasty in the future. Keywords Rhinoplasty Á Augmentation materials Á Poly(L-lactic-co-glycolic acid) Á Chondrocyte Á Tissue engineering Yoo Seob Shin and Chul-Ho Kim have been contributed equally to the study.

Nano Letters, 2019

Methods Supporting Figures S1-S12 Supporting Notes Supporting Information Movie 1-4 captions Meth... more Methods Supporting Figures S1-S12 Supporting Notes Supporting Information Movie 1-4 captions Methods Growth of monolayer MoS2 and fabrication of the sensor. Growth of monolayer MoS2 film was performed on Si/SiO2(300nm) substrate via metal-organic chemical vapor deposition (MOCVD). The reactor was equipped with 4.3-inch-diameter horizontal quartz tube and 3zone heating furnace. We used Mo(CO)6 (Sigma-Aldrich 577766) and (C2H5)2S (Sigma-Aldrich 107247) as transition metal and chalcogen source, respectively. The concentration of the precursors was regulated using mass flow controller along with argon gas acting as a diluting agent, which was kept in bubblers at a constant pressure of 800 Torr. Mo(CO)6 precursor was constantly heated to maintain 60 o C. The optimized flow rates are as follows: 0.6 sccm Mo(CO)6, 0.4 sccm (C2H5)2S, 1 sccm H2, 1000 sccm Ar. The growth temperature was kept 600 o C, and the growth pressure was maintained at 3.7 Torr throughout the process (14 hours). The recrystallized NaCl plate was loaded in the furnace to control the grain size and stitching between grains of the polycrystalline MoS2 film 1. A photoresist (SU-8 2000.5, MicroChem) and polymethyl methacrylate (PMMA) were successively spin-coated on the synthesized MoS2, and, based on the surface energy difference between MoS2 and SiO2, the PMMA/SU-8/MoS2 layer was delaminated from the SiO2/Si wafer by slowly soaking in water 2. Then, the PMMA/SU-8/MoS2 layer was transferred to a polyimide (PI, 25 μm) substrate and dried overnight. After the MoS2 was transferred onto the target substrate, the PMMA and SU-8 were removed by heated acetone (70 o C) and SU-8 developer respectively, and this was followed by cleaning with isopropyl alcohol (IPA) and distilled water. To isolate the channel, PMMA and SU-8 2002 negative photoresists were patterned photolithographically onto the transferred MoS2 monolayer, and the MoS2 was etched with reactive ion etching (RIE) system. Then, Ti/Au (10 nm/60 nm) were deposited thermally and photolithographically patterned, and the photoresists were dissolved and removed by PG (MicroChem). Then, the negative photoresist (SU-8 2002) was patterned as a spacer to form an air dielectric layer. PDMS (10:1

Current Topic in Lactic Acid Bacteria and Probiotics, 2013

Lactic Acid Bacteria(LAB) produces lactic acid as the major metabolic end-product of carbohydrate... more Lactic Acid Bacteria(LAB) produces lactic acid as the major metabolic end-product of carbohydrate fermentation. LAB offers health benefits such as increasing nutrients, enhancing immune system, alleviating lactose intolerance and allergy. In order to understand the characteristics of fermented milk and market trends, contents of LAB on fermented milk in Korea was investigated and isolated LAB was identified. Viable counts were performed on plate count agar with bromocresol purple(BCP) for LAB except Bifidobacterium and BS agar for Bifidobacterium. As the results, all of fermented milk in Korea contains viable LAB more than 1×10 8 CFU/mL, Korea Food Standard for concentrated fermented milk. To identify the isolated LAB, gram stain was performed with pure culture colonies on MRS agar. Gram positive streptococci, bacilli, and variable bacilli were observed through the microscope. The isolated LAB were further identified by API CHL for lactobacilli and other LAB, by API 20A for anaerobic bacteria. As the results, Gram positive streptococci was Streptococcus thermophilus(99.2%) and variable bacilli was Bifidobacterium spp.(96.2%) isolated from sample 1-10. Gram positive bacilli was Lactobacillus rhamnosus(99.8%) isolated only sample 2.

Korean journal for food science of animal resources, 2019

Nucleotides play important roles in numerous intracellular biochemical processes and are used in ... more Nucleotides play important roles in numerous intracellular biochemical processes and are used in infant formulas and other dairy products. However, domestic analytical methods for assessing nucleotide content in products have not yet been established, and therefore, methods for determining nucleotide content are urgently required. A rapid and simple analytical method for determining the content of five types of nucleotides in dairy products was improved using solid phase extraction clean-up and high-performance liquid chromatography with diode array detector. The extraction solvent used in the AOAC method was not well dissolved and was changed to hydrophilic EDTA-Na. In addition, the results obtained using the isocratic elution method and a single wavelength were similar to those obtained using the AOAC method, and the time taken for analysis was shortened from 40 min to 25 min. The process of method validation revealed the following parameters: accuracy (84.69%-102.72%), precision (1.51%-6.82%), linearity (0.999), and limit of detection (cytidine 5′-monophosphate, 0.09 mg/L; uridine 5′-monophosphate, 0.11 mg/L; adenosine 5′-monophosphate, 0.12 mg/L; guanosine 5′-monophosphate, 0.11 mg/L; and inosine 5′-monophosphate, 0.14 mg/L). The method was also used to determine the nucleotide concentration in 25 samples (infant formulas, 1.99-29.39 mg/100 g; and cow milk, 0.28-0.83 mg/100 g). The newly improved method was appropriate for analyzing nucleotides in infant formulas and other dairy products faster when compared to conventional methods.

Journal of Colloid and Interface Science, 2018

Defects in bone are some of the most difficult injuries to treat. Biomimetic scaffolds represent ... more Defects in bone are some of the most difficult injuries to treat. Biomimetic scaffolds represent a promising approach for successful bone tissue regeneration. In this study, a three-dimensional (3D) scaffold with osteo-inductive functionality was designed and assayed both in-vitro and in-vivo. Bone formation peptide-1 (BFP1), an osteo-promoting specific peptide, was covalently bound to a 3D printed polycaprolactone (PCL) scaffold using polydopamine (DOPA). The amount of BFP1 immobilized on the surface was found to increase depending on the BFP1 concentration of the loading solution. To observe the biological effects of the 3D scaffolds, human tonsil-derived mesenchymal stem cells (hTMSCs) were isolated. The cells were cultured on the scaffolds and observed to rapidly differentiate into osteoblast-like cells with osteo-promoting capabilities. The scaffolds were implanted in a rabbit calvarial defect model for 8 weeks and successfully stimulated both vessel and bone regeneration. Osteo-promoting 3D scaffolds may provide a safer and more efficient approach for bone repair and remodelling in regenerative medicine.

Materials Today, 2015

Over the past few years, attention has been focused on the therapeutic roles in designing bone sc... more Over the past few years, attention has been focused on the therapeutic roles in designing bone scaffolds for successful repair and regeneration. Indeed, biologically dynamic events in the bone healing process involve many of the molecules and cells adherent to the scaffold. Recent bone scaffolds have been designed considering intrinsic chemical and physical factors and exogenous/extrinsic cues that induce bone regeneration. Here, we attempt to topically review the current trends and to suggest featured strategies for the design of therapeutically relevant bone scaffolds taking into account recent studies and applications.

BioResearch open access, 2012

A novel bone tissue-engineering construct was developed by using poly(ɛ-caprolactone) (PCL)-macro... more A novel bone tissue-engineering construct was developed by using poly(ɛ-caprolactone) (PCL)-macrochanneled scaffolds combined with stem cell-seeded collagen hydrogels and then applying flow perfusion culture. Rat mesenchymal stem cells (MSCs) were loaded into collagen hydrogels, which were then combined with macrochanneled PCL scaffolds. Collagen hydrogels were demonstrated to provide favorable growth environments for MSCs and to foster proliferation. Cell number determination identified retention of substantially fewer (50-60%) cells when they were seeded directly onto macrochanneled PCL than of cells engineered within collagen hydrogels. Additionally, the cells actively proliferated within the combined scaffold for up to 7 days. MSC-loaded collagen-PCL scaffolds were subsequently cultured under flow perfusion to promote proliferation and osteogenic differentiation. Cells proliferated to levels significantly higher in flow perfusion culture than that under static conditions during ...

Surface and Coatings Technology, 2014

ABSTRACT We prepared chitosan–gelatin (Chi–Gel) composite coatings on Ti via electrophoretic depo... more ABSTRACT We prepared chitosan–gelatin (Chi–Gel) composite coatings on Ti via electrophoretic deposition (EPD) method for utilization in tissue repair and drug delivery. Uniform coatings were produced over a wide compositional range (0–75% Gel) with coating gains dependent on the EPD parameters including voltage and time. Coating degradation increased as the Gel content increased, with 16–54% weight losses after 3 weeks of immersion in phosphate buffered saline. Ampicillin, used as a model drug, was successfully incorporated within the coatings during the EPD process, and the release was highly sustainable with no burst effect up to a month, proving the potential of these materials as long-term drug eluting coatings. The release rate was dependent on the coating degradation, i.e., the more degradable with increasing Gel content, suggesting a rate-controllable drug release by a compositional change. Preliminary cell tests showed favorable cell adhesion and spreading on the composite coatings, with significant improvement in cell proliferation as Gel content increased. While more in-depth biological assays remain, the Chi–Gel might be useful as a drug eluting electrophoretic coating system on metallic implants for tissue repair.

Journal of tissue engineering, Jan 7, 2010

Fibroblast growth factors (FGFs) that signal through FGF receptors (FGFRs) regulate a broad spect... more Fibroblast growth factors (FGFs) that signal through FGF receptors (FGFRs) regulate a broad spectrum of biological functions, including cellular proliferation, survival, migration, and differentiation. The FGF signal pathways are the RAS/MAP kinase pathway, PI3 kinase/AKT pathway, and PLCγ pathway, among which the RAS/MAP kinase pathway is known to be predominant. Several studies have recently implicated the in vitro biological functions of FGFs for tissue regeneration. However, to obtain optimal outcomes in vivo, it is important to enhance the half-life of FGFs and their biological stability. Future applications of FGFs are expected when the biological functions of FGFs are potentiated through the appropriate use of delivery systems and scaffolds. This review will introduce the biology and cellular functions of FGFs and deal with the biomaterials based delivery systems and their current applications for the regeneration of tissues, including skin, blood vessel, muscle, adipose, ten...

In vivo (Athens, Greece)

A novel nanofibrous membrane of a degradable biopolymer poly (lactide-co-ε-caprolactone) (PLCL) f... more A novel nanofibrous membrane of a degradable biopolymer poly (lactide-co-ε-caprolactone) (PLCL) for guided bone regeneration (GBR) was designed and its tissue compatibility and ability to promote the regeneration of new bone were investigated in a rat mandibular defect model. The nanofibrous structuring of the PLCL polymer was facilitated by a solvent-induced phase separation method using camphene as the porogen. The PLCL membrane was implanted in a critical-sized (5 mm diameter) defect of the rat mandible. The assessment of cell compatibility conducted using undifferentiated pre-osteoblast cells (MC3T3-E1) showed favorable cell adhesion and growth on the nanofiber PLCL membrane with an active cytoskeletal processes and increment in the cell population with culture time. In vivo results at four weeks post-operation demonstrated that the PLCL nanofibrous membrane induced better guided new bone formation than the defect control group while protecting the bone defect against the ingrow...

Biotechnology Letters, 2014

Regenerative Medicine, 2012

Growth factors (GFs) such as BMPs, FGFs, VEGFs and IGFs have significant impacts on osteoblast be... more Growth factors (GFs) such as BMPs, FGFs, VEGFs and IGFs have significant impacts on osteoblast behavior, and thus have been widely utilized for bone tissue regeneration. Recently, securing biological stability for a sustainable and controllable release to the target tissue has been a challenge to practical applications. This challenge has been addressed to some degree with the development of appropriate carrier materials and delivery systems. This review highlights the importance and roles of those GFs, as well as their proper administration for targeting bone regeneration. Additionally, the in vitro and in vivo performance of those GFs with or without the use of carrier systems in the repair and regeneration of bone tissue is systematically addressed. Moreover, some recent advances in the utility of the GFs, such as using fusion technology, are also reviewed.

Yonsei Medical Journal, 2006

Nanoscale, 2012

Inorganic bioactive nanomaterials are attractive for hard tissue regeneration, including nanocomp... more Inorganic bioactive nanomaterials are attractive for hard tissue regeneration, including nanocomponents for bone replacement composites and nanovehicles for delivering therapeutics. Bioactive glass nanoparticles (BGn) have recently gained potential usefulness as bone and tooth regeneratives. Here we demonstrate the capacity of the BGn with mesopores to load and deliver therapeutic molecules (drugs and particularly genes). Spherical BGn with sizes of 80-90 nm were produced to obtain 3-5 nm sized mesopores through a sono-reacted sol-gel process. A simulated body fluid test of the mesoporous BGn confirmed their excellent apatite forming ability and the cellular toxicity study demonstrated their good cell viability up to 100 μg ml(-1). Small molecules like chemical drug (Na-ampicillin) and gene (small interfering RNA; siRNA) were introduced as model drugs considering the mesopore size of the nanoparticles. Moreover, amine-functionalization allowed switchable surface charge property of the BGn (from -20-30 mV to +20-30 mV). Loading of ampicillin or siRNA saturated within a few hours (~2 h) and reflected the mesopore structure. While the ampicillin released relatively rapidly (~12 h), the siRNA continued to release up to 3 days with almost zero-order kinetics. The siRNA-nanoparticles were easily taken up by the cells, with a transfection efficiency as high as ~80%. The silencing effect of siRNA delivered from the BGn, as examined by using bcl-2 model gene, showed dramatic down-regulation (~15% of control), suggesting the potential use of BGn as a new class of nanovehicles for genes. This, in conjunction with other attractive properties, including size- and mesopore-related high surface area and pore volume, tunable surface chemistry, apatite-forming ability, good cell viability and the possible ion-related stimulatory effects, will potentiate the usefulness of the BGn in hard tissue regeneration.

Materials Letters, 2011

ABSTRACT Here we prepared tricalcium phosphate (TCP) spherical cups of a few micrometers in size ... more ABSTRACT Here we prepared tricalcium phosphate (TCP) spherical cups of a few micrometers in size (average=2.74μm) by applying the electrospraying method. The sol–gel precursor containing calcium and phosphate (Ca/P=1.5) was mixed with Polyvinylpyrrolidone in ethanol at varying concentrations, and then sprayed under a controlled electrostatic field. The jets from the sol–gel solution changed from a complete fibrous mesh into a formation of microbeads connected with fibers and then further into a complete formation of microbeads as the solution viscosity became decreased. After the thermal treatment (800–1100°C), individual microbeads remained, to form the shape of a spherical cup and retained the phase of β-TCP. The drug delivery potential of the TCP micro-cups, as assessed by using an antibiotic drug ampicillin, demonstrated a dose dependent loading capacity and almost a linear release profile over a day.

Materials Letters, 2010

Microspherical particles have attracted great interest as a delivery system of tissue cells in re... more Microspherical particles have attracted great interest as a delivery system of tissue cells in regenerative engineering. For hard tissue regeneration, here we exploited porous biomedical composite microspheres of hydroxyapatite–polycaprolactone (HA–PCL). The pore channels within the microsphere was facilitated by using camphene as the porogen, where the initial HA–PCL–camphene mixture in chloroform was solidified and the camphene was then sublimed to