Jorge Tortora - Academia.edu (original) (raw)

Papers by Jorge Tortora

Archives of virology, Jan 8, 2017

Bovine leukemia virus (BLV) was detected and genotyped in a population of 201 dairy cattle from c... more Bovine leukemia virus (BLV) was detected and genotyped in a population of 201 dairy cattle from central Mexico. Using a commercial indirect enzyme-linked immunosorbent assay (iELISA) kit, 118 polymerase chain reaction (PCR)-positive and BLV antibody-positive samples were identified; the concordance between tests was substantial. A phylogenetic study of 27 partial sequences of the env gene gp30 was performed. Four mutations were detected involving the PXXP motif in the cytoplasmic domain of the transmembrane protein. This study provided evidence of the efficacy of PCR for the detection of BLV and demonstrated the presence of genotype 1 BLV in Mexico.

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Redvet Revista Electronica De Veterinaria, 2013

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Veterinaria Mexico

The presence of antibodies against Maedi-Visna (MV) and caprine arthritis encephalitis (CAE) was ... more The presence of antibodies against Maedi-Visna (MV) and caprine arthritis encephalitis (CAE) was evaluated in ovine and caprine fetuses obtained from pregnant females slaughtered in abattoirs. Sera from 54 caprine fetuses and 65 ovine fetuses were collected and evaluated by the indirect ELISA technique. Antibodies were detected in five caprine fetuses of 80 days of gestation and in four ovine fetuses of 90 to 100 days of gestation; three caprine fetuses and two ovine fetuses of 60 to 65 days of gestation were suspicious. The possibility of uterine infection with these retroviruses is confirmed, and its consequences in current disease control and eradication strategies are discussed.

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Journal of Veterinary Behavior, 2015

ABSTRACT Romanov ewes give birth to an average of 2.8 lambs per partum and display maternal abili... more ABSTRACT Romanov ewes give birth to an average of 2.8 lambs per partum and display maternal ability over other sheep breeds; however, few studies have investigated their mother–young mutual recognition. Here, 8 primiparous and 7 maternally experienced (multiparous) ewes and their lambs were studied. Maternal selectivity was evaluated at 3 hours postpartum during two consecutive periods of 3 minutes each. Ewes were tested with their own lambs and then with alien lambs. High- and low-pitched bleats, attempts and rejection to the udder, aggressive behavior toward the lambs, and amount of time that the mother permits the lamb to be near the udder were recorded. At 8 hours postpartum, a double-choice test was performed to assess distal recognition of the lamb by the mother; in the lambs, a double-choice test was performed at 24 hours of age. Latency to reach one of the animals, frequency to visit each, time spent near the choice subject, time spent looking toward them, and frequency of vocalization were recorded. A Mann–Whitney U-test was used for between-group comparisons, and a Wilcoxon test was used for within-group comparisons. At 3 hours postpartum, most of the ewes (multiparous and primiparous) were selective; however, multiparous ewes tended to have a higher index of acceptance of their own lambs than primiparous ewes (2.74 ± 0.66 vs 0.56 ± 0.76, P = 0.083). At 8 hours postpartum, multiparous ewes spent more time looking toward their own lambs than primiparous ewes (50 ± 11.8 vs. 15.8 ± 8.9 s, P = 0.018). Compared to primiparous ewes, multiparous ewes spent more time near their own lambs than near alien lambs (84.85 ± 19.6 vs. 21.7 ± 9.7 s, P = 0.05), and more time looking toward their own lambs than toward alien lambs (P = 0.018). At 24 hours after birth, lambs born to multiparous ewes spent more time near their own mothers than near alien mothers (P = 0.043) and visited their own mothers more than they visited alien mothers (P = 0.05). In contrast, lambs born to primiparous ewes did not show similar behaviors (P ≥ 0.73). In conclusion, in Romanov sheep, maternal experience affects mostly non olfactory recognition of the lamb and also influences the ability of the lamb to recognize its mother.

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Pesquisa Veterinária Brasileira, 2014

The pathogens of the reproductive system in the male can penetrate and establish by ascending rou... more The pathogens of the reproductive system in the male can penetrate and establish by ascending route, from to the prepuce to the urethra, accessory glands, epididymis and testicles. The aim of this paper is determine the distribution and number of cells involved in the immune response in prepuce and pelvic urethra of rams, without apparent clinical alterations in testicle, epididymis and prepuce. The distribution of some of the cells involved in the immune response at the level of the prepuce and the pelvic urethra was quantified in four one-year-old rams seronegative for B. ovis and A. seminis and without apparent lesions in the testicles, the epididymis, and the prepuce. At the moment of slaughter, samples were taken from the preputial fornix and the pelvic urethra and placed in 10% formalin and under freezing conditions. CD4, CD8, WC1, CD45RO, CD14 and CD1b cells were demonstrated by immunohistochemistry, and immunoglobulin-containing cells (ICC) of the IgA, IgG and IgM classes we...

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Veterinaria …, 2009

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El objetivo del trabajo fue determinar el balance de Se y la respuesta en la glutation peroxidasa... more El objetivo del trabajo fue determinar el balance de Se y la respuesta en la glutation peroxidasa. Se llevo a cabo un ensayo para evaluar el efecto de la concentracion de Se en la dieta, asi como la adicion de Se organico, los tratamientos fueron: T1) Dieta testigo, la cual contenia 0.33 mg de Se y T2) Testigo + 300 mg de Selplex, con una concentracion final de 0.93 mg de Se. El ensayo se llevo a cabo usando 8 corderos con un peso inicial de 30.5 ± 2.09 kg, donde la concentracion de Se en tejidos, la actividad de glutation peroxidasa y balance de Se fueron analizados. El Se en la dieta no afecto la concentracion de este en tejidos para el grupo testigo asi como para los animales que recibieron Se organico. La actividad de glutation peroxidasa no tuvo diferencias entre tratamientos (p <= 0.11) aunque disminuyo ligeramente conforme transcurrieron los dias del experimento, tanto en el testigo como en el tratamiento con Se organico. Los animales que recibieron Se organico tuvieron un...

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Revista Mexicana de Ciencias Pecuarias, 2006

Con el objetivo de inducir la infeccion experimental de A. seminis, se utilizaron 18 corderos de ... more Con el objetivo de inducir la infeccion experimental de A. seminis, se utilizaron 18 corderos de seis meses de edad: 4 testigos negativos, otros 3 se inocularon con A. seminis via intraepididimal (IE) y 11 fueron inoculados por via intrauretral (IU) como sigue: cuatro recibieron factor liberador de gonadotropinas (GnRH) cinco dias previos al desafio, cuatro recibieron etilenglicol por via IE 24 h previas al desafio y tres no recibieron tratamiento previo. De los cuatro testigos negativos, dos recibieron GnRH y dos etilenglicol. Se tomaron muestras semanales de suero para inmunodifusion, y de semen para bacteriologia. Se sacrificaron a los 35 dias posinoculacion, obteniendose testiculos, epididimos y glandulas anexas, para estudios bacteriologicos e histopatologicos. Del grupo tratado con GnRH, se recupero la bacteria del semen pero no del aparato reproductor, aunque se observaron lesiones en 3 de 4 animales. Del grupo estimulado con etilenglicol, en uno se aislo A. seminis de ampula...

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Revista Mexicana de Ciencias Pecuarias, 2013

La epididimitis causada por Brucella ovis es una enfermedad de importancia en los rebanos ovinos ... more La epididimitis causada por Brucella ovis es una enfermedad de importancia en los rebanos ovinos de Mexico. En los sementales afectados ocasiona baja productividad por la disminucion de la fertilidad. El objetivo del estudio fue determinar la relevancia de diferentes posibles factores de riesgo (sistema de produccion, densidad de sementales, total de vientres, relacion hembras:macho, sistema de empadre y raza del semental) sobre la prevalencia de B. ovis en Zacatecas, Mexico. Se muestrearon 544 sementales, en 153 rebanos, provenientes de cuatro sistemas de produccion. La respuesta serologica se evaluo mediante inmunodifusion doble en gel. El 18.6 % (101/544) de los sementales muestreados resultaron positivos y el 10.5 % (16/153) de los rebanos presento al menos un semental positivo. El sistema semi-intensivo presento la mayor prevalencia (P<0.05), con 86.1 % (87/101) de sementales positivos, el extensivo 11.9 % (12/101), el traspatio 2.0 % (2/101), y el intensivo no registro...

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PARA ESTABLECER FACTORES QUE INFLUYEN EN EL PESO AL NACIMIENTO (PN) A LOS 30 D Y 60 DIAS, SE UTIL... more PARA ESTABLECER FACTORES QUE INFLUYEN EN EL PESO AL NACIMIENTO (PN) A LOS 30 D Y 60 DIAS, SE UTILIZARON LOS REGISTROS DE 917 CORDEROS COLUMBIA, PRODUCTO DE UN SISTEMA DE APAREAMIENTO ANUAL (S-A) CON PARTOS EN ABRIL Y UNO CADA OCHO MESES (S-I), CON PARTOS EN ABRIL-MAYO (A-M), DICIEMBRE-ENERO (D-E) Y AGOSTO-SEPTIEMBRE (A-S), BAJO CONDICIONES DE PASTOREO INTENSIVO EN PRADERAS IRRIGADAS DE CLIMA TEMPLADO. DESDE LOS 15 DIAS, LOS CORDEROS DISPUSIERON DE SUPLEMENTO. EN EL ANALISIS LOS EFECTOS FIJOS INCLUYERON: EDAD DE LA MADRE, TIPO DE PARTO, SEXO Y DENTRO DE SISTEMA, EPOCA O ANO. SE REALIZARON CORRELACIONES LINEALES ENTRE PESOS AL NACER 30 Y 60 DIAS Y LA CONDICION CORPORAL DE LAS MADRES. EL PN (5.7 ± 0.06 KG) Y A 30 D (15.0 ± 0.17 KG) DEL S-I FUE MAYOR (P 0.05), PERO SI A LOS 30 DIAS (14.7 ± 0.22 KG VS. 13.2 ± 0.18 KG). EN EL S-I, LOS NACIDOS EN D-E FUERON MAS PESADOS (P < 0.05) AL NACIMIENTO 30 DIAS Y 60 DIAS QUE A-M Y A-S (6.2 ± 0.08 KG, 5.3 ± 0.10 KG Y 5.5 ± 0.09 KG; 16.2 ± 0.22 KG,...

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A routine andrology and semen examination was carried out on a group of 12, one-year old, Pelibue... more A routine andrology and semen examination was carried out on a group of 12, one-year old, Pelibuey breed rams, but one individual had persisting leukocytospermia. No clinical alterations of the reproductive system were found in any of the rams. At slaughter of the problem animal, the seminal vesicles were found to be markedly lobulated and larger than normal, as were the vas deferens ampoules. When dissected, both structures contained creamy white yellowish material. Histopathology demonstrated ampullitis and seminal vesiculitis. A Gram-negative pleomorphic bacillus was isolated from both of the organs, which was characterized through the API 20E system and PCR. It was found to behave similar to the reference strain for Actinobacillus seminis ATCC 15768.

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Revista Mexicana de Ciencias Pecuarias

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Journal of Animal …, 2007

In experimentally infected rams with <I>Actiobacillus seminis,</I> pathological chang... more In experimentally infected rams with <I>Actiobacillus seminis,</I> pathological changes (reproductive organs) and antibodies (IgM, IgG and IgA) in serum, seminal plasma and smegma were evaluated. Twelve 1-year old rams were infected via intraurethal IU group (n = 4) and ...

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Development, 1973

1. Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) th... more 1. Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) through a hole in the shell. A control group was injected with normal saline, a second group with INH plus vitamin B6, and a third group was left unopened. 2. INH, in the doses we used, proved to be lethal for more than 50% of the early chick embryos, and also produced important developmental alterations. 3. Alterations produced by INH were primarily observed at the level of the neural epithelium, particularly at its cephalic portion. The most important ones were a degenerative and necrotic process of the neural epithelium, and a distortion of the normal anatomical relations of the cephalic structures. 4. The embryonic mortality and developmental alterations induced by INH were prevented to a considerable extent by the concurrent injection of pyridoxine hydrochloride and INH. 5. The preventive action of vitamin B6 suggests that toxicity of INH in the chicken embryos is due to the antivitam...

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Cells Tissues Organs, 1971

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Cells Tissues Organs, 1975

New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by mean... more New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by means of a tiny hole in the shell. A control group was injected with normal saline and a second group was left unopened. Iproniazid in the doses we used proved to be lethal for approximately 50% of the chick embryos, while we cannot demonstrate other developmental alterations, either in the macroscopical or in the serial histological study.

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J. Anim. Vet. …, 2008

... Effect of Pre-and Postpartum Selenium Supplementation in Sheep !Abd Elghany Hefnawy, !R. Lópe... more ... Effect of Pre-and Postpartum Selenium Supplementation in Sheep !Abd Elghany Hefnawy, !R. López-Arellano, 'A. Revilla-Vázquez, ]E. Ramírez ... appropriate blood concentration to prevent Se-deficient associated syndromes (Paulson et al, 1968; Horton et al, 1978; Loren et al ...

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Bronchial epithelium from Mycoplasma hyopneumoniae experimentally infected pigs was studied by us... more Bronchial epithelium from Mycoplasma hyopneumoniae experimentally infected pigs was studied by using immunofluorescence. Colonization was analyzed during twenty days post-infection. Twelve one-month-old pigs were intratracheally inoculated with a Mycoplasma hyopneumoniae-infected lung homogenate. Pigs were sacrificed on days 1, 4, 8, 12, 16 and 20 post-infection, two each day; six control animals were inoculated with Friis media. Pneumonic lesions were evaluated and analyzed by M. hyopneumoniae isolation and histological studies. Direct immunofluorescence was performed to determined colonization. Fluorescence was positive starting on day four; cilium clumping was evident from day eight. Mycoplasma installation started in a spot of the bronchial mucosa and extended to form a full fluorescence ring on day 20.

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Archives of virology, Jan 8, 2017

Bovine leukemia virus (BLV) was detected and genotyped in a population of 201 dairy cattle from c... more Bovine leukemia virus (BLV) was detected and genotyped in a population of 201 dairy cattle from central Mexico. Using a commercial indirect enzyme-linked immunosorbent assay (iELISA) kit, 118 polymerase chain reaction (PCR)-positive and BLV antibody-positive samples were identified; the concordance between tests was substantial. A phylogenetic study of 27 partial sequences of the env gene gp30 was performed. Four mutations were detected involving the PXXP motif in the cytoplasmic domain of the transmembrane protein. This study provided evidence of the efficacy of PCR for the detection of BLV and demonstrated the presence of genotype 1 BLV in Mexico.

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Redvet Revista Electronica De Veterinaria, 2013

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Veterinaria Mexico

The presence of antibodies against Maedi-Visna (MV) and caprine arthritis encephalitis (CAE) was ... more The presence of antibodies against Maedi-Visna (MV) and caprine arthritis encephalitis (CAE) was evaluated in ovine and caprine fetuses obtained from pregnant females slaughtered in abattoirs. Sera from 54 caprine fetuses and 65 ovine fetuses were collected and evaluated by the indirect ELISA technique. Antibodies were detected in five caprine fetuses of 80 days of gestation and in four ovine fetuses of 90 to 100 days of gestation; three caprine fetuses and two ovine fetuses of 60 to 65 days of gestation were suspicious. The possibility of uterine infection with these retroviruses is confirmed, and its consequences in current disease control and eradication strategies are discussed.

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Journal of Veterinary Behavior, 2015

ABSTRACT Romanov ewes give birth to an average of 2.8 lambs per partum and display maternal abili... more ABSTRACT Romanov ewes give birth to an average of 2.8 lambs per partum and display maternal ability over other sheep breeds; however, few studies have investigated their mother–young mutual recognition. Here, 8 primiparous and 7 maternally experienced (multiparous) ewes and their lambs were studied. Maternal selectivity was evaluated at 3 hours postpartum during two consecutive periods of 3 minutes each. Ewes were tested with their own lambs and then with alien lambs. High- and low-pitched bleats, attempts and rejection to the udder, aggressive behavior toward the lambs, and amount of time that the mother permits the lamb to be near the udder were recorded. At 8 hours postpartum, a double-choice test was performed to assess distal recognition of the lamb by the mother; in the lambs, a double-choice test was performed at 24 hours of age. Latency to reach one of the animals, frequency to visit each, time spent near the choice subject, time spent looking toward them, and frequency of vocalization were recorded. A Mann–Whitney U-test was used for between-group comparisons, and a Wilcoxon test was used for within-group comparisons. At 3 hours postpartum, most of the ewes (multiparous and primiparous) were selective; however, multiparous ewes tended to have a higher index of acceptance of their own lambs than primiparous ewes (2.74 ± 0.66 vs 0.56 ± 0.76, P = 0.083). At 8 hours postpartum, multiparous ewes spent more time looking toward their own lambs than primiparous ewes (50 ± 11.8 vs. 15.8 ± 8.9 s, P = 0.018). Compared to primiparous ewes, multiparous ewes spent more time near their own lambs than near alien lambs (84.85 ± 19.6 vs. 21.7 ± 9.7 s, P = 0.05), and more time looking toward their own lambs than toward alien lambs (P = 0.018). At 24 hours after birth, lambs born to multiparous ewes spent more time near their own mothers than near alien mothers (P = 0.043) and visited their own mothers more than they visited alien mothers (P = 0.05). In contrast, lambs born to primiparous ewes did not show similar behaviors (P ≥ 0.73). In conclusion, in Romanov sheep, maternal experience affects mostly non olfactory recognition of the lamb and also influences the ability of the lamb to recognize its mother.

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Pesquisa Veterinária Brasileira, 2014

The pathogens of the reproductive system in the male can penetrate and establish by ascending rou... more The pathogens of the reproductive system in the male can penetrate and establish by ascending route, from to the prepuce to the urethra, accessory glands, epididymis and testicles. The aim of this paper is determine the distribution and number of cells involved in the immune response in prepuce and pelvic urethra of rams, without apparent clinical alterations in testicle, epididymis and prepuce. The distribution of some of the cells involved in the immune response at the level of the prepuce and the pelvic urethra was quantified in four one-year-old rams seronegative for B. ovis and A. seminis and without apparent lesions in the testicles, the epididymis, and the prepuce. At the moment of slaughter, samples were taken from the preputial fornix and the pelvic urethra and placed in 10% formalin and under freezing conditions. CD4, CD8, WC1, CD45RO, CD14 and CD1b cells were demonstrated by immunohistochemistry, and immunoglobulin-containing cells (ICC) of the IgA, IgG and IgM classes we...

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Veterinaria …, 2009

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El objetivo del trabajo fue determinar el balance de Se y la respuesta en la glutation peroxidasa... more El objetivo del trabajo fue determinar el balance de Se y la respuesta en la glutation peroxidasa. Se llevo a cabo un ensayo para evaluar el efecto de la concentracion de Se en la dieta, asi como la adicion de Se organico, los tratamientos fueron: T1) Dieta testigo, la cual contenia 0.33 mg de Se y T2) Testigo + 300 mg de Selplex, con una concentracion final de 0.93 mg de Se. El ensayo se llevo a cabo usando 8 corderos con un peso inicial de 30.5 ± 2.09 kg, donde la concentracion de Se en tejidos, la actividad de glutation peroxidasa y balance de Se fueron analizados. El Se en la dieta no afecto la concentracion de este en tejidos para el grupo testigo asi como para los animales que recibieron Se organico. La actividad de glutation peroxidasa no tuvo diferencias entre tratamientos (p <= 0.11) aunque disminuyo ligeramente conforme transcurrieron los dias del experimento, tanto en el testigo como en el tratamiento con Se organico. Los animales que recibieron Se organico tuvieron un...

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Revista Mexicana de Ciencias Pecuarias, 2006

Con el objetivo de inducir la infeccion experimental de A. seminis, se utilizaron 18 corderos de ... more Con el objetivo de inducir la infeccion experimental de A. seminis, se utilizaron 18 corderos de seis meses de edad: 4 testigos negativos, otros 3 se inocularon con A. seminis via intraepididimal (IE) y 11 fueron inoculados por via intrauretral (IU) como sigue: cuatro recibieron factor liberador de gonadotropinas (GnRH) cinco dias previos al desafio, cuatro recibieron etilenglicol por via IE 24 h previas al desafio y tres no recibieron tratamiento previo. De los cuatro testigos negativos, dos recibieron GnRH y dos etilenglicol. Se tomaron muestras semanales de suero para inmunodifusion, y de semen para bacteriologia. Se sacrificaron a los 35 dias posinoculacion, obteniendose testiculos, epididimos y glandulas anexas, para estudios bacteriologicos e histopatologicos. Del grupo tratado con GnRH, se recupero la bacteria del semen pero no del aparato reproductor, aunque se observaron lesiones en 3 de 4 animales. Del grupo estimulado con etilenglicol, en uno se aislo A. seminis de ampula...

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Revista Mexicana de Ciencias Pecuarias, 2013

La epididimitis causada por Brucella ovis es una enfermedad de importancia en los rebanos ovinos ... more La epididimitis causada por Brucella ovis es una enfermedad de importancia en los rebanos ovinos de Mexico. En los sementales afectados ocasiona baja productividad por la disminucion de la fertilidad. El objetivo del estudio fue determinar la relevancia de diferentes posibles factores de riesgo (sistema de produccion, densidad de sementales, total de vientres, relacion hembras:macho, sistema de empadre y raza del semental) sobre la prevalencia de B. ovis en Zacatecas, Mexico. Se muestrearon 544 sementales, en 153 rebanos, provenientes de cuatro sistemas de produccion. La respuesta serologica se evaluo mediante inmunodifusion doble en gel. El 18.6 % (101/544) de los sementales muestreados resultaron positivos y el 10.5 % (16/153) de los rebanos presento al menos un semental positivo. El sistema semi-intensivo presento la mayor prevalencia (P<0.05), con 86.1 % (87/101) de sementales positivos, el extensivo 11.9 % (12/101), el traspatio 2.0 % (2/101), y el intensivo no registro...

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PARA ESTABLECER FACTORES QUE INFLUYEN EN EL PESO AL NACIMIENTO (PN) A LOS 30 D Y 60 DIAS, SE UTIL... more PARA ESTABLECER FACTORES QUE INFLUYEN EN EL PESO AL NACIMIENTO (PN) A LOS 30 D Y 60 DIAS, SE UTILIZARON LOS REGISTROS DE 917 CORDEROS COLUMBIA, PRODUCTO DE UN SISTEMA DE APAREAMIENTO ANUAL (S-A) CON PARTOS EN ABRIL Y UNO CADA OCHO MESES (S-I), CON PARTOS EN ABRIL-MAYO (A-M), DICIEMBRE-ENERO (D-E) Y AGOSTO-SEPTIEMBRE (A-S), BAJO CONDICIONES DE PASTOREO INTENSIVO EN PRADERAS IRRIGADAS DE CLIMA TEMPLADO. DESDE LOS 15 DIAS, LOS CORDEROS DISPUSIERON DE SUPLEMENTO. EN EL ANALISIS LOS EFECTOS FIJOS INCLUYERON: EDAD DE LA MADRE, TIPO DE PARTO, SEXO Y DENTRO DE SISTEMA, EPOCA O ANO. SE REALIZARON CORRELACIONES LINEALES ENTRE PESOS AL NACER 30 Y 60 DIAS Y LA CONDICION CORPORAL DE LAS MADRES. EL PN (5.7 ± 0.06 KG) Y A 30 D (15.0 ± 0.17 KG) DEL S-I FUE MAYOR (P 0.05), PERO SI A LOS 30 DIAS (14.7 ± 0.22 KG VS. 13.2 ± 0.18 KG). EN EL S-I, LOS NACIDOS EN D-E FUERON MAS PESADOS (P < 0.05) AL NACIMIENTO 30 DIAS Y 60 DIAS QUE A-M Y A-S (6.2 ± 0.08 KG, 5.3 ± 0.10 KG Y 5.5 ± 0.09 KG; 16.2 ± 0.22 KG,...

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A routine andrology and semen examination was carried out on a group of 12, one-year old, Pelibue... more A routine andrology and semen examination was carried out on a group of 12, one-year old, Pelibuey breed rams, but one individual had persisting leukocytospermia. No clinical alterations of the reproductive system were found in any of the rams. At slaughter of the problem animal, the seminal vesicles were found to be markedly lobulated and larger than normal, as were the vas deferens ampoules. When dissected, both structures contained creamy white yellowish material. Histopathology demonstrated ampullitis and seminal vesiculitis. A Gram-negative pleomorphic bacillus was isolated from both of the organs, which was characterized through the API 20E system and PCR. It was found to behave similar to the reference strain for Actinobacillus seminis ATCC 15768.

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Revista Mexicana de Ciencias Pecuarias

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Journal of Animal …, 2007

In experimentally infected rams with <I>Actiobacillus seminis,</I> pathological chang... more In experimentally infected rams with <I>Actiobacillus seminis,</I> pathological changes (reproductive organs) and antibodies (IgM, IgG and IgA) in serum, seminal plasma and smegma were evaluated. Twelve 1-year old rams were infected via intraurethal IU group (n = 4) and ...

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Development, 1973

1. Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) th... more 1. Unincubated White Leghorn fertile eggs were injected with isonicotinic acid hydrazide (INH) through a hole in the shell. A control group was injected with normal saline, a second group with INH plus vitamin B6, and a third group was left unopened. 2. INH, in the doses we used, proved to be lethal for more than 50% of the early chick embryos, and also produced important developmental alterations. 3. Alterations produced by INH were primarily observed at the level of the neural epithelium, particularly at its cephalic portion. The most important ones were a degenerative and necrotic process of the neural epithelium, and a distortion of the normal anatomical relations of the cephalic structures. 4. The embryonic mortality and developmental alterations induced by INH were prevented to a considerable extent by the concurrent injection of pyridoxine hydrochloride and INH. 5. The preventive action of vitamin B6 suggests that toxicity of INH in the chicken embryos is due to the antivitam...

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Cells Tissues Organs, 1971

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Cells Tissues Organs, 1975

New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by mean... more New Hampshire eggs were injected with iproniazid into the yolk sac at day 2 of incubation by means of a tiny hole in the shell. A control group was injected with normal saline and a second group was left unopened. Iproniazid in the doses we used proved to be lethal for approximately 50% of the chick embryos, while we cannot demonstrate other developmental alterations, either in the macroscopical or in the serial histological study.

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J. Anim. Vet. …, 2008

... Effect of Pre-and Postpartum Selenium Supplementation in Sheep !Abd Elghany Hefnawy, !R. Lópe... more ... Effect of Pre-and Postpartum Selenium Supplementation in Sheep !Abd Elghany Hefnawy, !R. López-Arellano, 'A. Revilla-Vázquez, ]E. Ramírez ... appropriate blood concentration to prevent Se-deficient associated syndromes (Paulson et al, 1968; Horton et al, 1978; Loren et al ...

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Bronchial epithelium from Mycoplasma hyopneumoniae experimentally infected pigs was studied by us... more Bronchial epithelium from Mycoplasma hyopneumoniae experimentally infected pigs was studied by using immunofluorescence. Colonization was analyzed during twenty days post-infection. Twelve one-month-old pigs were intratracheally inoculated with a Mycoplasma hyopneumoniae-infected lung homogenate. Pigs were sacrificed on days 1, 4, 8, 12, 16 and 20 post-infection, two each day; six control animals were inoculated with Friis media. Pneumonic lesions were evaluated and analyzed by M. hyopneumoniae isolation and histological studies. Direct immunofluorescence was performed to determined colonization. Fluorescence was positive starting on day four; cilium clumping was evident from day eight. Mycoplasma installation started in a spot of the bronchial mucosa and extended to form a full fluorescence ring on day 20.

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