José Antonio Garcia Lillo - Academia.edu (original) (raw)

Papers by José Antonio Garcia Lillo

Applied and Environmental Microbiology, 1990

The halobacterium Haloferax mediterranei accumulates poly(β-hydroxybutyrate) (PHB) as intracellul... more The halobacterium Haloferax mediterranei accumulates poly(β-hydroxybutyrate) (PHB) as intracellular granules. The conditions for PHB production in batch and continuous cultures have been studied and optimized. Phosphate limitation is essential for PHB accumulation in large quantities. Glucose and starch are the best carbon sources. With 2% starch, 0.00375% KH 2 PO 4 , and 0.2% NH 4 Cl in batch culture, a production of ca. 6 g of PHB per liter was reached, being 60% of the total biomass dry weight, and giving a yield over the carbon source of 0.33 g/g. The PHB production in continuous cultures was stable over a 3-month period. Our results demonstrate that H. mediterranei is an interesting candidate for industrial production of biological polyesters.

General and Applied Aspects of Halophilic Microorganisms, 1991

The halobacterium H. mediterranei is an organism of biotechnological potential due to its capacit... more The halobacterium H. mediterranei is an organism of biotechnological potential due to its capacity as polymer producer. This microorganism accumulates poly-s-hydroxybutyrate as intracellular granules in very large amounts. PHB is an interesting biopolymer for its possible use as biodegradable thermoplastic. The conditions of culture for PHB production have been studied and optimized in batch as well as in continuous culture. Phosphate limitation is an essential condition for PHB accumulation in important quantities. Glucose and starch were the carbon sources giving the highest productivities. Under favourable circumstances in batch culture a production of 6.5 g/1 PHB was reached, being 67% of the total biomass dry weight, and with a yield over the carbon source of 0.33 gg-1. There is evidence indicating that the PHB produced by H. mediterranei is a copolymer with monomers of more than four carbon atoms, which is favourable for the manipulation of the physical properties of the polymer. H. mediterranei accumulates another polymer extracellularly. It is a sulfated heteropolysaccharide that has very good rheological properties which are very resistant to environmental stress including, as may be expected, salinity. High productivities of this polysaccharide require sugars as carbon source, a condition that also favours PHB accumulation.

FEMS Microbiology Letters, 1992

Didáctica de las Ciencias Experimentales y Sociales, 2015

Mostrar que una nueva propuesta de enseñanza produce mejores actitudes y aprendizajes en los alum... more Mostrar que una nueva propuesta de enseñanza produce mejores actitudes y aprendizajes en los alumnos requiere disponer de un análisis de lo que se hace y se consigue con la enseñanza habitual. Para realizar dicho análisis se ha efectuado un estudio histórico y epistemológico de la evolución de las ideas en genética clásica, identificando los problemas que están en su origen, las ideas que permitieron avanzar y los obstáculos que hubo que superar. Como resultado de dicho estudio se han seleccionado un conjunto de indicadores de aprendizaje que deberían manifestarse en aquellas personas que hubieran comprendido los aspectos esenciales del modelo de herencia mendeliana, que se imparte en 4º de ESO. Dichos indicadores se han utilizado para analizar el aprendizaje tras la enseñanza convencional del tema. En este trabajo se presentan los resultados obtenidos que muestran las deficiencias más comunes y justifican la necesidad de una propuesta diferente. Palabras clave: didáctica de las ciencias, enseñanza habitual, genética clásica, modelo hereditario de Mendel, enseñanza de las ciencias en la E.S.O.

Applied and Environmental Microbiology, 1990

The halobacterium Haloferax mediterranei accumulates poly(β-hydroxybutyrate) (PHB) as intracellul... more The halobacterium Haloferax mediterranei accumulates poly(β-hydroxybutyrate) (PHB) as intracellular granules. The conditions for PHB production in batch and continuous cultures have been studied and optimized. Phosphate limitation is essential for PHB accumulation in large quantities. Glucose and starch are the best carbon sources. With 2% starch, 0.00375% KH 2 PO 4 , and 0.2% NH 4 Cl in batch culture, a production of ca. 6 g of PHB per liter was reached, being 60% of the total biomass dry weight, and giving a yield over the carbon source of 0.33 g/g. The PHB production in continuous cultures was stable over a 3-month period. Our results demonstrate that H. mediterranei is an interesting candidate for industrial production of biological polyesters.

General and Applied Aspects of Halophilic Microorganisms, 1991

The halobacterium H. mediterranei is an organism of biotechnological potential due to its capacit... more The halobacterium H. mediterranei is an organism of biotechnological potential due to its capacity as polymer producer. This microorganism accumulates poly-s-hydroxybutyrate as intracellular granules in very large amounts. PHB is an interesting biopolymer for its possible use as biodegradable thermoplastic. The conditions of culture for PHB production have been studied and optimized in batch as well as in continuous culture. Phosphate limitation is an essential condition for PHB accumulation in important quantities. Glucose and starch were the carbon sources giving the highest productivities. Under favourable circumstances in batch culture a production of 6.5 g/1 PHB was reached, being 67% of the total biomass dry weight, and with a yield over the carbon source of 0.33 gg-1. There is evidence indicating that the PHB produced by H. mediterranei is a copolymer with monomers of more than four carbon atoms, which is favourable for the manipulation of the physical properties of the polymer. H. mediterranei accumulates another polymer extracellularly. It is a sulfated heteropolysaccharide that has very good rheological properties which are very resistant to environmental stress including, as may be expected, salinity. High productivities of this polysaccharide require sugars as carbon source, a condition that also favours PHB accumulation.

FEMS Microbiology Letters, 1992

Didáctica de las Ciencias Experimentales y Sociales, 2015

Mostrar que una nueva propuesta de enseñanza produce mejores actitudes y aprendizajes en los alum... more Mostrar que una nueva propuesta de enseñanza produce mejores actitudes y aprendizajes en los alumnos requiere disponer de un análisis de lo que se hace y se consigue con la enseñanza habitual. Para realizar dicho análisis se ha efectuado un estudio histórico y epistemológico de la evolución de las ideas en genética clásica, identificando los problemas que están en su origen, las ideas que permitieron avanzar y los obstáculos que hubo que superar. Como resultado de dicho estudio se han seleccionado un conjunto de indicadores de aprendizaje que deberían manifestarse en aquellas personas que hubieran comprendido los aspectos esenciales del modelo de herencia mendeliana, que se imparte en 4º de ESO. Dichos indicadores se han utilizado para analizar el aprendizaje tras la enseñanza convencional del tema. En este trabajo se presentan los resultados obtenidos que muestran las deficiencias más comunes y justifican la necesidad de una propuesta diferente. Palabras clave: didáctica de las ciencias, enseñanza habitual, genética clásica, modelo hereditario de Mendel, enseñanza de las ciencias en la E.S.O.