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Papers by José Maria Zabay

Research paper thumbnail of Serum �2-Microglobulin and Prediction of Progression to AIDS in HIV-Infected Injection Drug Users

Journal of Acquired Immune Deficiency Syndromes Human Retrovirology, 1995

Research paper thumbnail of Severe combined immunodeficiency with T lymphocytes retaining functional activity

Clinical Immunology and Immunopathology, 1988

Two cases of severe combined immunodeficiency (SCID) with normal numbers of T cells are reported.... more Two cases of severe combined immunodeficiency (SCID) with normal numbers of T cells are reported. Studies of T-cell subsets showed an absence of TQ1+ lymphocytes and a very low percentage of CD4+ cells in Patient 2. Functional studies of T cells from this patient showed a normal suppressor activity. Patient 1 had normal percentages of T-cell subsets and his lymphocytes showed helper and suppressor activities but to a lesser degree than normal controls. Both cases stressed the heterogeneity of SCID in which T cells could be present and retain some of their functional activities.

Research paper thumbnail of Severe combined immunodeficiency with disbalance and functional abnormalities in the T-lymphocyte subsets

Clinical Immunology and Immunopathology, 1982

The case of an infant female with severe infections and failure to thrive is reported. Immunologi... more The case of an infant female with severe infections and failure to thrive is reported. Immunological workup demonstrated a severe combined immunodeficiency with normal numbers of circulating T and B lymphocytes. Studies of T-cell subsets showed an absence of T,+ cells, while the percentage of T4+ exceeded that of T3+ cells. Functional studies of the T4+ subset demonstrated lack of helper activity. When cocultured with normal T cells, B cells from the patient produced in vitro subnormal amounts of IgM, but practically no quantities of IgG and IgA. A lymph node biopsy showed a preserved T-cell-dependent area. Possible pathogenetic mechanisms are discussed.

Research paper thumbnail of Serum β2-Microglobulin and Prediction of Progression to AIDS in HIV-Infected Injection Drug Users

Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology, 1995

ABSTRACT

Research paper thumbnail of Immunological abnormalities in primary APS evolving into SLE: 6 years follow-up in women with repeated pregnancy loss

Lupus, 1999

We have performed a prospective study to determine the prevalence of immunological abnormalities ... more We have performed a prospective study to determine the prevalence of immunological abnormalities and the evolution from primary antiphospholipid syndrome (APS) into systemic lupus erythematosus (SLE) in women who had had unexplained repeated pregnancy loss (PL) and APS. Of 105 women with abortions or fetal deaths, 33(31%) fulfilled criteria for APS. Among these patients with primary APS, 24% had antinuclear antibodies (ANA), 91% had elevated circulating immune complexes (CIC), 70% had low total haemolytic complement (CH100), 52% had low levels of complement 4 (C4) and 30% had low levels of complement 3 (C3), in a significantly higher prevalence than women whose pregnancies were successful (control group). Through out a 6 y follow-up, 3 (9%) of the patients with APS who had autoimmune related abnormalities when entered into the study developed features of lupus like disease (LLD) or fullblown SLE. Our findings suggest that women with unexplained repeated PL with APS who presented wit...

Research paper thumbnail of Cyclooxygenase and lipoxygenase arachidonic acid metabolism by monocytes from human immune deficiency virus-infected drug users

Journal of Chromatography A, 1991

Prostaglandin E2, thromboxane B2 and leukotriene B4 monocyte production have been determined in h... more Prostaglandin E2, thromboxane B2 and leukotriene B4 monocyte production have been determined in human immune deficiency virus (HIV)-infected drug users (n = 36) and healthy subjects (n = 29). Eicosanoids were extracted from the incubates using C18 solid-phase cartridges and determined by radioimmunoassay. An enhanced production of prostaglandin E2 and thromboxane B2 was detected in monocytes from HIV-positive drug users whether or not they had been previously stimulated with zymosan. Concomitant leukotriene B4 increases were not observed. The results reported in this paper indicate that altered cyclooxygenase arachidonic acid metabolism in monocytes from HIV-infected drug users is associated with the severe cellular immunodysfunction characteristic of AIDS. In contrast, leukotriene B4 does not seem to play a role in AIDS-associated immunosuppression.

Research paper thumbnail of Peripheral T-cell lymphoma a clinical, histologic, and immunologic study of five cases

Cancer, 1985

The authors describe five white patients with peripheral T-cell lymphoma. Four patients were olde... more The authors describe five white patients with peripheral T-cell lymphoma. Four patients were older than 65 years. All cases presented with a short clinical course and advanced stage at the time of diagnosis. Clinical manifestations included asthenia, weight loss, peripheral and abdominal lymphadenopathy. One case showed tonsillar involvement and subcutaneous lymph node enlargement; hepatomegaly was present in four cases, two of them with splenomegaly. Only one case presented peripheral lymphocytosis and antibodies to human T-leukemia virus. Although three cases were classified as diffuse mixed lymphomas and two as poorly differentiated lymphocytic lymphomas, there were some common characteristics: diffuse infiltration by different proportions of small lymphoid cells and large immunoblasts, some of them multinucleated and similar to Reed-Sternberg cells; accumulation of histiocytes, plasmacytosis, eosinophilia, venular proliferation and compartmentalization were also found. Bone marrow infiltration was observed in two patients. Results of monoclonal markers showed four cases to be OKT4+ and the other OKT8+. The morphologic and immunologic characteristics of these patients were typical and similar to those reported from other geographical areas.

Research paper thumbnail of Immunological and serological markers predictive of progression to AIDS in a cohort of HIV-infected drug users

Research paper thumbnail of Quantification of low levels of human immunodeficiency virus (HIV) type 1 RNA in P24 antigen-negative, asymptomatic, HIV-positive patients by PCR

Journal of Clinical Microbiology, 1996

A nested PCR was used to quantify small numbers of human immunodeficiency virus (HIV) type 1 (HIV... more A nested PCR was used to quantify small numbers of human immunodeficiency virus (HIV) type 1 (HIV-1) RNA particles in the serum specimens of 26 p24 antigen-negative, asymptomatic, HIV-positive patients undergoing antiretroviral therapy. Fifteen patients received zidovudine (ZDV) and alpha interferon, and 11 patients received ZDV monotherapy. After PCR, the amounts of RNA were quantified by comparing the endpoint dilutions of serum samples with a standard curve with known amounts of viral particles. Before the beginning of the antiviral therapy, HIV-1 RNA was detected in 92% of the patients. After treatment, a fall in the number of viral particles was detected in patients receiving combination therapy (mean titers +/- standard errors of the means, 3,617 +/- 756 pretherapy versus 1,800 +/- 845 posttherapy; P < 0.05) and in patients receiving monotherapy (3,763 +/- 642 pretherapy versus 1,353 +/- 394 posttherapy; P < 0.05). Our results indicate that PCR with nested primers may be...

Research paper thumbnail of Serum �2-Microglobulin and Prediction of Progression to AIDS in HIV-Infected Injection Drug Users

Journal of Acquired Immune Deficiency Syndromes Human Retrovirology, 1995

Research paper thumbnail of Severe combined immunodeficiency with T lymphocytes retaining functional activity

Clinical Immunology and Immunopathology, 1988

Two cases of severe combined immunodeficiency (SCID) with normal numbers of T cells are reported.... more Two cases of severe combined immunodeficiency (SCID) with normal numbers of T cells are reported. Studies of T-cell subsets showed an absence of TQ1+ lymphocytes and a very low percentage of CD4+ cells in Patient 2. Functional studies of T cells from this patient showed a normal suppressor activity. Patient 1 had normal percentages of T-cell subsets and his lymphocytes showed helper and suppressor activities but to a lesser degree than normal controls. Both cases stressed the heterogeneity of SCID in which T cells could be present and retain some of their functional activities.

Research paper thumbnail of Severe combined immunodeficiency with disbalance and functional abnormalities in the T-lymphocyte subsets

Clinical Immunology and Immunopathology, 1982

The case of an infant female with severe infections and failure to thrive is reported. Immunologi... more The case of an infant female with severe infections and failure to thrive is reported. Immunological workup demonstrated a severe combined immunodeficiency with normal numbers of circulating T and B lymphocytes. Studies of T-cell subsets showed an absence of T,+ cells, while the percentage of T4+ exceeded that of T3+ cells. Functional studies of the T4+ subset demonstrated lack of helper activity. When cocultured with normal T cells, B cells from the patient produced in vitro subnormal amounts of IgM, but practically no quantities of IgG and IgA. A lymph node biopsy showed a preserved T-cell-dependent area. Possible pathogenetic mechanisms are discussed.

Research paper thumbnail of Serum β2-Microglobulin and Prediction of Progression to AIDS in HIV-Infected Injection Drug Users

Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology, 1995

ABSTRACT

Research paper thumbnail of Immunological abnormalities in primary APS evolving into SLE: 6 years follow-up in women with repeated pregnancy loss

Lupus, 1999

We have performed a prospective study to determine the prevalence of immunological abnormalities ... more We have performed a prospective study to determine the prevalence of immunological abnormalities and the evolution from primary antiphospholipid syndrome (APS) into systemic lupus erythematosus (SLE) in women who had had unexplained repeated pregnancy loss (PL) and APS. Of 105 women with abortions or fetal deaths, 33(31%) fulfilled criteria for APS. Among these patients with primary APS, 24% had antinuclear antibodies (ANA), 91% had elevated circulating immune complexes (CIC), 70% had low total haemolytic complement (CH100), 52% had low levels of complement 4 (C4) and 30% had low levels of complement 3 (C3), in a significantly higher prevalence than women whose pregnancies were successful (control group). Through out a 6 y follow-up, 3 (9%) of the patients with APS who had autoimmune related abnormalities when entered into the study developed features of lupus like disease (LLD) or fullblown SLE. Our findings suggest that women with unexplained repeated PL with APS who presented wit...

Research paper thumbnail of Cyclooxygenase and lipoxygenase arachidonic acid metabolism by monocytes from human immune deficiency virus-infected drug users

Journal of Chromatography A, 1991

Prostaglandin E2, thromboxane B2 and leukotriene B4 monocyte production have been determined in h... more Prostaglandin E2, thromboxane B2 and leukotriene B4 monocyte production have been determined in human immune deficiency virus (HIV)-infected drug users (n = 36) and healthy subjects (n = 29). Eicosanoids were extracted from the incubates using C18 solid-phase cartridges and determined by radioimmunoassay. An enhanced production of prostaglandin E2 and thromboxane B2 was detected in monocytes from HIV-positive drug users whether or not they had been previously stimulated with zymosan. Concomitant leukotriene B4 increases were not observed. The results reported in this paper indicate that altered cyclooxygenase arachidonic acid metabolism in monocytes from HIV-infected drug users is associated with the severe cellular immunodysfunction characteristic of AIDS. In contrast, leukotriene B4 does not seem to play a role in AIDS-associated immunosuppression.

Research paper thumbnail of Peripheral T-cell lymphoma a clinical, histologic, and immunologic study of five cases

Cancer, 1985

The authors describe five white patients with peripheral T-cell lymphoma. Four patients were olde... more The authors describe five white patients with peripheral T-cell lymphoma. Four patients were older than 65 years. All cases presented with a short clinical course and advanced stage at the time of diagnosis. Clinical manifestations included asthenia, weight loss, peripheral and abdominal lymphadenopathy. One case showed tonsillar involvement and subcutaneous lymph node enlargement; hepatomegaly was present in four cases, two of them with splenomegaly. Only one case presented peripheral lymphocytosis and antibodies to human T-leukemia virus. Although three cases were classified as diffuse mixed lymphomas and two as poorly differentiated lymphocytic lymphomas, there were some common characteristics: diffuse infiltration by different proportions of small lymphoid cells and large immunoblasts, some of them multinucleated and similar to Reed-Sternberg cells; accumulation of histiocytes, plasmacytosis, eosinophilia, venular proliferation and compartmentalization were also found. Bone marrow infiltration was observed in two patients. Results of monoclonal markers showed four cases to be OKT4+ and the other OKT8+. The morphologic and immunologic characteristics of these patients were typical and similar to those reported from other geographical areas.

Research paper thumbnail of Immunological and serological markers predictive of progression to AIDS in a cohort of HIV-infected drug users

Research paper thumbnail of Quantification of low levels of human immunodeficiency virus (HIV) type 1 RNA in P24 antigen-negative, asymptomatic, HIV-positive patients by PCR

Journal of Clinical Microbiology, 1996

A nested PCR was used to quantify small numbers of human immunodeficiency virus (HIV) type 1 (HIV... more A nested PCR was used to quantify small numbers of human immunodeficiency virus (HIV) type 1 (HIV-1) RNA particles in the serum specimens of 26 p24 antigen-negative, asymptomatic, HIV-positive patients undergoing antiretroviral therapy. Fifteen patients received zidovudine (ZDV) and alpha interferon, and 11 patients received ZDV monotherapy. After PCR, the amounts of RNA were quantified by comparing the endpoint dilutions of serum samples with a standard curve with known amounts of viral particles. Before the beginning of the antiviral therapy, HIV-1 RNA was detected in 92% of the patients. After treatment, a fall in the number of viral particles was detected in patients receiving combination therapy (mean titers +/- standard errors of the means, 3,617 +/- 756 pretherapy versus 1,800 +/- 845 posttherapy; P < 0.05) and in patients receiving monotherapy (3,763 +/- 642 pretherapy versus 1,353 +/- 394 posttherapy; P < 0.05). Our results indicate that PCR with nested primers may be...