José Nart - Academia.edu (original) (raw)
Papers by José Nart
Clinical Implant Dentistry and Related Research
Orthodontics & Craniofacial Research
Journal of Periodontology
Journal of Periodontology
Journal of Periodontology
Journal of Oral Microbiology
Introduction: Antibiotic resistance is widely found even among bacterial populations not having b... more Introduction: Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods: Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively. Results: tet(M) was the most frequently detected tet gene (52.03%). We observed significant differences between the prevalence of tet(M), tet(W), tet(O), tet(32) and tet(L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83.64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups. Conclusion: The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota.
The International Journal of Periodontics & Restorative Dentistry
The significance of keratinized mucosa around dental implants for the prevention of biologic comp... more The significance of keratinized mucosa around dental implants for the prevention of biologic complications has been a subject of controversy. Agreement, however, exists on the benefits provided to achieve more satisfactory oral hygiene measures and reduced clinical inflammation. A prospective interventional case series of 14 patients (31 implants) were examined every 3 months for up to 12 months. The effect of soft tissue conditioning by means of free autologous epithelial graft on the management of peri-implantitis with supracrestal and/or dehiscence-type defect morphology was evaluated. All clinical parameters were significantly reduced (P < .001), with complete disease resolution in 78.6% of the patients and 87.1% of the peri-implantitis implants. Unsuccessful cases were associated with less gain of keratinized mucosa, deep probing pocket depths, bleeding on probing, and less satisfaction during brushing at 12 months. Dimensional changes following soft tissue grafting were more significant during the first 3 months and led to a 42.4% shrinkage at 12 months. Soft tissue conditioning by means of free autologous epithelial graft in combination with apically positioned flap is a viable and effective therapy to manage peri-implantitis associated with deficient keratinized mucosa.
Journal of Periodontology
Current Oral Health Reports
Journal of Periodontology
Clinical Oral Investigations
Clinical Implant Dentistry and Related Research
Background The present case series assesses the response to reconstructive therapy for the manage... more Background The present case series assesses the response to reconstructive therapy for the management of 2 3 wall peri implantitis bone defects following submerged healing guided bone regeneration Material and Methods Fifteen consecutive patients with 27 implants presenting peri implantitis were included Guided bone regeneration was applied by means of autoge nous bone/deproteinized bovine bone mineral grafting mixture and collagen membrane Patients were assessed at baseline (T 0 and at 6 (T 1 and 12 months (T 2 Clinical and radiographic variables defined the composite success criteria (probing pocket depth [ 5 mm, no bleeding on probing/suppuration ( no further radiographic bone loss) Patient site specific comfort was scored using a visual analog scale ( Descrip tive statistics was carried out to assess the changes along the study period Outcomes are reported in terms of mean values 5 95 percentile values) Results All the clinical variables substantially changed from T 0 through T 2 In particular, PPD decreased 3 7 mm 0 7 5 9 from T 0 to T 2 Likewise, the scores for the modified plaque index ( and modified sulcular bleeding index ( were reduced by 0 5 0 5 1 1 and 1 6 0 4 2 4 respectively SUP did not display at any implant site at T 2 59 2 implants in 29 2 patients suppurated at T 0 Keratinized mucosa decreased 0 6 mm 0 2 4 4 and while mucosal recession increased 2 5 mm 1 0 4 3 Alike, the radiographic parameters varied significantly from T 0 through T 2 Infrabony defects were filled by 2 2 mm 0 0 8 6 at T 2 and marginal bone loss was reduced by 2 3 mm 1 1 8 1 The mean VAS score significantly increased from T 0 56 7 through T 1 to T 2 reaching a score of 96 at T 2 At this timepoint, 85 2 of the peri implantitis lesions were resolved Conclusions The proposed surgical approach followed by submerged healing to reconstruct peri implant bone defects may offer one therapeutic option for failing dental implants Given the nature of the present study, its effectiveness in compari son to less invasive treatments needs investigation in randomized controlled trials
Journal of Clinical Periodontology
Journal of Clinical Periodontology
THE INTERNATIONAL JOURNAL OF PERIODONTICS AND RESTORATIVE DENTISTRY
Journal of Clinical Periodontology
The International Journal of Periodontics & Restorative Dentistry
This study sought to evaluate gingival volume changes following root coverage with the vestibular... more This study sought to evaluate gingival volume changes following root coverage with the vestibular incision subperiosteal tunnel access (VISTA) procedure. Pre-and postoperative surface scans of 21 patients (154 teeth) treated with VISTA using various graft materials were digitally superimposed to quantify volumetric changes. A linear gingival thickness gain of approximately 1 mm and volumetric gain of 5.47 mm 3 were achieved. A negative correlation was found between linear thickness gain and root prominence. The thickness achieved was not different with various graft materials. Since gingival thickness has been identified as an important predictor of periodontal root coverage, the methodology described in the present study, along with the identification of predictors of outcome, has important therapeutic implications. Int J
Journal of Oral Microbiology
Tetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal... more Tetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal protection proteins and inactivation enzymes. Some of these genes have only been described in either Gram-positive or Gram-negative bacteria. This is the case of tet(B), which codes for an efflux pump and, so far, had only been found in Gram-negative bacteria. In this study, tet(B) was detected in two clinical Streptococcus oralis strains isolated from the gingival sulci of two subjects. In both cases, the gene was completely sequenced, yielding 100% shared identity and coverage with other previously published sequences of tet(B). Moreover, we studied the expression of tet(B) using RT-qPCR in the isolates grown with and without tetracycline, detecting constitutive expression in only one of the isolates, with no signs of expression in the other one. This is the first time that the presence and expression of the tet(B) gene has been confirmed in Gram-positive bacteria, which highlights the potential of the genus Streptococcus to become a reservoir and a disseminator of antibiotic resistance genes in an environment so prone to horizontal gene transfer as is the oral biofilm.
Journal of Clinical Periodontology
Journal of Periodontology
Clinical Implant Dentistry and Related Research
Orthodontics & Craniofacial Research
Journal of Periodontology
Journal of Periodontology
Journal of Periodontology
Journal of Oral Microbiology
Introduction: Antibiotic resistance is widely found even among bacterial populations not having b... more Introduction: Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles. Methods: Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively. Results: tet(M) was the most frequently detected tet gene (52.03%). We observed significant differences between the prevalence of tet(M), tet(W), tet(O), tet(32) and tet(L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83.64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups. Conclusion: The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota.
The International Journal of Periodontics & Restorative Dentistry
The significance of keratinized mucosa around dental implants for the prevention of biologic comp... more The significance of keratinized mucosa around dental implants for the prevention of biologic complications has been a subject of controversy. Agreement, however, exists on the benefits provided to achieve more satisfactory oral hygiene measures and reduced clinical inflammation. A prospective interventional case series of 14 patients (31 implants) were examined every 3 months for up to 12 months. The effect of soft tissue conditioning by means of free autologous epithelial graft on the management of peri-implantitis with supracrestal and/or dehiscence-type defect morphology was evaluated. All clinical parameters were significantly reduced (P < .001), with complete disease resolution in 78.6% of the patients and 87.1% of the peri-implantitis implants. Unsuccessful cases were associated with less gain of keratinized mucosa, deep probing pocket depths, bleeding on probing, and less satisfaction during brushing at 12 months. Dimensional changes following soft tissue grafting were more significant during the first 3 months and led to a 42.4% shrinkage at 12 months. Soft tissue conditioning by means of free autologous epithelial graft in combination with apically positioned flap is a viable and effective therapy to manage peri-implantitis associated with deficient keratinized mucosa.
Journal of Periodontology
Current Oral Health Reports
Journal of Periodontology
Clinical Oral Investigations
Clinical Implant Dentistry and Related Research
Background The present case series assesses the response to reconstructive therapy for the manage... more Background The present case series assesses the response to reconstructive therapy for the management of 2 3 wall peri implantitis bone defects following submerged healing guided bone regeneration Material and Methods Fifteen consecutive patients with 27 implants presenting peri implantitis were included Guided bone regeneration was applied by means of autoge nous bone/deproteinized bovine bone mineral grafting mixture and collagen membrane Patients were assessed at baseline (T 0 and at 6 (T 1 and 12 months (T 2 Clinical and radiographic variables defined the composite success criteria (probing pocket depth [ 5 mm, no bleeding on probing/suppuration ( no further radiographic bone loss) Patient site specific comfort was scored using a visual analog scale ( Descrip tive statistics was carried out to assess the changes along the study period Outcomes are reported in terms of mean values 5 95 percentile values) Results All the clinical variables substantially changed from T 0 through T 2 In particular, PPD decreased 3 7 mm 0 7 5 9 from T 0 to T 2 Likewise, the scores for the modified plaque index ( and modified sulcular bleeding index ( were reduced by 0 5 0 5 1 1 and 1 6 0 4 2 4 respectively SUP did not display at any implant site at T 2 59 2 implants in 29 2 patients suppurated at T 0 Keratinized mucosa decreased 0 6 mm 0 2 4 4 and while mucosal recession increased 2 5 mm 1 0 4 3 Alike, the radiographic parameters varied significantly from T 0 through T 2 Infrabony defects were filled by 2 2 mm 0 0 8 6 at T 2 and marginal bone loss was reduced by 2 3 mm 1 1 8 1 The mean VAS score significantly increased from T 0 56 7 through T 1 to T 2 reaching a score of 96 at T 2 At this timepoint, 85 2 of the peri implantitis lesions were resolved Conclusions The proposed surgical approach followed by submerged healing to reconstruct peri implant bone defects may offer one therapeutic option for failing dental implants Given the nature of the present study, its effectiveness in compari son to less invasive treatments needs investigation in randomized controlled trials
Journal of Clinical Periodontology
Journal of Clinical Periodontology
THE INTERNATIONAL JOURNAL OF PERIODONTICS AND RESTORATIVE DENTISTRY
Journal of Clinical Periodontology
The International Journal of Periodontics & Restorative Dentistry
This study sought to evaluate gingival volume changes following root coverage with the vestibular... more This study sought to evaluate gingival volume changes following root coverage with the vestibular incision subperiosteal tunnel access (VISTA) procedure. Pre-and postoperative surface scans of 21 patients (154 teeth) treated with VISTA using various graft materials were digitally superimposed to quantify volumetric changes. A linear gingival thickness gain of approximately 1 mm and volumetric gain of 5.47 mm 3 were achieved. A negative correlation was found between linear thickness gain and root prominence. The thickness achieved was not different with various graft materials. Since gingival thickness has been identified as an important predictor of periodontal root coverage, the methodology described in the present study, along with the identification of predictors of outcome, has important therapeutic implications. Int J
Journal of Oral Microbiology
Tetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal... more Tetracycline resistance can be achieved through tet genes, which code for efflux pumps, ribosomal protection proteins and inactivation enzymes. Some of these genes have only been described in either Gram-positive or Gram-negative bacteria. This is the case of tet(B), which codes for an efflux pump and, so far, had only been found in Gram-negative bacteria. In this study, tet(B) was detected in two clinical Streptococcus oralis strains isolated from the gingival sulci of two subjects. In both cases, the gene was completely sequenced, yielding 100% shared identity and coverage with other previously published sequences of tet(B). Moreover, we studied the expression of tet(B) using RT-qPCR in the isolates grown with and without tetracycline, detecting constitutive expression in only one of the isolates, with no signs of expression in the other one. This is the first time that the presence and expression of the tet(B) gene has been confirmed in Gram-positive bacteria, which highlights the potential of the genus Streptococcus to become a reservoir and a disseminator of antibiotic resistance genes in an environment so prone to horizontal gene transfer as is the oral biofilm.
Journal of Clinical Periodontology
Journal of Periodontology