José Sifuentes-osornio - Academia.edu (original) (raw)
Papers by José Sifuentes-osornio
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
... José Sifuentes Osornio, Miriam Bobadilla del Valle, Midori Kato Maeda, María Guadalupe Matus ... more ... José Sifuentes Osornio, Miriam Bobadilla del Valle, Midori Kato Maeda, María Guadalupe Matus Ruíz, Areli Martínez Gamboa, Susana Maldonado, Pablo Méndez, Barbara Chávez Mazari, Alfonso Ponce de León, María de Lourdes García García, Manuel Palacios Martíne. ...
Journal of Clinical Microbiology
The purpose of this open and multicenter trial was to determine the usefulness of antibody detect... more The purpose of this open and multicenter trial was to determine the usefulness of antibody detection by enzyme-linked immunosorbent assay (ELISA) in gingival crevicular transudate (GCT), which was collected with an investigational device (Orasure; Epitope, Beaverton, Oreg.), for the diagnosis of human immunodeficiency virus type 1 (HIV-1) infection and to compare it with antibody detection in serum. There were two false-negative gingival samples, one from an HIV-1-positive asymptomatic individual and one from a patient with AIDS. HIV-1 antibodies were unexpectedly detected in both serum and GCT of two individuals, one with dental disease and one with pulmonary tuberculosis. None of the sera or GCTs from healthy subjects or patients with AD were positive. Compared with the serum assay, the sensitivity, specificity, and positive and negative predictive values of the GCT assay were 99.5, 100, 100, and 99.9%, respectively. Of 355 paired serum-GCT samples that were HIV-1 positive by ELISA and that
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
To describe the epidemiology and prognosis of P. aeruginosa bloodstream infections in a tertiary-... more To describe the epidemiology and prognosis of P. aeruginosa bloodstream infections in a tertiary-care center. Retrospective analysis. Tertiary-care teaching hospital in Mexico City. All cases of P. aeruginosa bacteremia diagnosed from 1981 to 1994. Relevant demographic, clinical and therapeutic variables were analyzed. A total of 153 bacteremias were found between 1981 and 1994, with a mean prevalence of 4.1 episodes per 1000 hospital discharges. Twenty-five percent of the infections derived from the biliary tract, and the most frequent underlying diseases were hematologic malignancies. The overall crude mortality was 46% (70/153) whereas mortality in the nosocomially-acquired episodes was 47% (58/124). Mortality within the first 72 h was 24% (37/153). A multivariate analysis showed six risk factors associated with a fatal outcome: age > or = 40 years, shock, mechanical ventilation, prior use of antibiotics, splenectomy and inappropriate selection of antibiotics. The identification of risk factors, and therefore a prompt instauration of specific antibiotic therapy, improved the prognosis of these severely ill patients.
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
To determine the clinical manifestations associated with resistant M. tuberculosis infection and ... more To determine the clinical manifestations associated with resistant M. tuberculosis infection and the antimicrobial resistance in isolates from Mexican patients. Epidemiological surveillance. Tuberculosis confirmed cases. Primary resistance: no history of treatment prior to diagnosis. The following critical concentrations (micrograms/mL) were used for susceptibility: isoniazid 0.2 and 1; rifampin 1 and 5; ethambutol 5 and 10; streptomycin 2 and 10; ethionamide 5; kanamycin 6; and para-aminosalicylic acid (PAS) 2 and 10. Eighty-four patients with a mean age of 44.7 years were included; 54 men (64%) and 30 women (36%); most patients were from the Mexico City metropolitan area. In 34 patients there was clinical information available, 26 presented fever and weight loss and 8 respiratory symptoms. Fifty-nine patients (70%) were infected by pan-susceptible M. tuberculosis, and 25 (30%) by a resistant isolate; 17 (68%) of them were resistant to at least two drugs, 16 (64%) to isoniazid and rifampin. The proportion of resistance was: isoniazid 24%, rifampin 19%, streptomycin 12%, ethambutol 10%, PAS 9%, etionamide 7%, and kanamycin 6%. Of 47 patients without previous treatment, eight had a resistant microorganism (17%): 9% resistant to isoniazid, 6% to rifampin, 2% to streptomycin, 6% to PAS and 6% multiresistant. Of 37 patients with history of previous treatment for tuberculosis, 17 (46%) had a resistant isolate; 44% were resistant to isoniazid, 35% to rifampin, 24% to streptomycin, 19% to ethambutol, 12% to PAS and 35% multiresistant. Of the 84 patients, four were physicians infected by a resistant isolate, and seven HIV-infected patients, one with a multiresistant isolate, and another with isoniazid resistance. Antimicrobial resistance among M. tuberculosis is alarmingly high in Mexico City; these results emphasize the importance of case detection and early isolation of patients.
JAMA The Journal of the American Medical Association
... in such settings remain an open question.2 Alfredo Ponce de Leon, MD Peter M. Small, MD Stanf... more ... in such settings remain an open question.2 Alfredo Ponce de Leon, MD Peter M. Small, MD Stanford University Medical Center Stanford, Calif Jose Sifuentes-Osornio, MD Instituto Nacional de la Nutricion Salvador Zubiran Mexico City, Mexico 1. Frieden TR, Sherman LF, Maw ...
Page 1. Otras secciones de este sitio: ...
Journal of Clinical Microbiology
The present paper analyzes the histoplasmin electrophoretic profiles and the randomly amplified p... more The present paper analyzes the histoplasmin electrophoretic profiles and the randomly amplified polymorphic DNA (RAPD) patterns of the fungus Histoplasma capsulatum isolated from Mexican patients with AIDSassociated histoplasmosis. Clinical isolates from Guatemala, Colombia, and Panama, as well as H. capsulatum isolates from different sources in nature, were also processed. All histoplasmin samples shared four antigenic fractions of 200, 49, 10.5, and 8.5 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). According to their percentage of relatedness, based on SDS-PAGE histoplasmin electrophoretic image analysis, H. capsulatum isolates were divided in two groups: group A contained all AIDS-associated isolates studied and two human reference strains from Mexican histoplasmosis patients without AIDS; group B included bat guano, infected bat, and cock excreta isolates from the State of Guerrero, Mexico, plus three human histoplasmosis strains from Guatemala, Panama, and Colombia. Polymorphic DNA patterns evaluated by RAPD-PCR showed three major bands of 4.4, 3.2, and 2.3 kb in most H. capsulatum isolates studied. Four groups were related by DNA polymorphisms: group I was formed by most of the AIDS-associated H. capsulatum isolates studied, one human histoplasmosis strain from Colombia, two human reference strains from Mexican patients without AIDS, and one human histoplasmosis strain from Guatemala. Group II consisted of only a single strain from Panama. Group III included three strains: one from a Mexican patient with AIDS and two isolated from nature in Guerrero (cock excreta and bat guano). The last, group IV, consisted of only one strain isolated from an infected bat, captured in Guerrero. A tight relationship between phenotypic and genotypic characterization was observed, and both analyses could be useful tools for typing H. capsulatum from different sources and geographic origins.
PLOS Neglected Tropical Diseases, 2015
Mycobacterium tuberculosis causes the majority of tuberculosis (TB) cases in humans; however, in ... more Mycobacterium tuberculosis causes the majority of tuberculosis (TB) cases in humans; however, in developing countries, human TB caused by M. bovis may be frequent but undetected. Human TB caused by M. bovis is considered a zoonosis; transmission is mainly through consumption of unpasteurized dairy products, and it is less frequently attributed to animal-to-human or human-to-human contact. We describe the trends of M. bovis isolation from human samples and first-line drug susceptibility during a 15-year period in a referral laboratory located in a tertiary care hospital in Mexico City.
RESUMEN Antecedentes. La tuberculosis, declarada como emergencia mundial por la Organización Mund... more RESUMEN Antecedentes. La tuberculosis, declarada como emergencia mundial por la Organización Mundial de la Salud es aún un problema importante de salud pública en México, incluida entre las primeras 20 causas de muerte. Objetivo. Conocer el impacto de ...
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
García-García L, et al. Indoor pollution as risk factor for pulmonary TB. Rev Invest Clin 2009; 6... more García-García L, et al. Indoor pollution as risk factor for pulmonary TB. Rev Invest Clin 2009; 61 (5): 392-398 392 Revista de Investigación Clínica / Vol. 61, Núm. 5 / Septiembre-Octubre, 2009 / pp 392-398 Versión completa de este artículo disponible en internet: www.imbiomed.com.mx
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
... José Sifuentes Osornio, Miriam Bobadilla del Valle, Midori Kato Maeda, María Guadalupe Matus ... more ... José Sifuentes Osornio, Miriam Bobadilla del Valle, Midori Kato Maeda, María Guadalupe Matus Ruíz, Areli Martínez Gamboa, Susana Maldonado, Pablo Méndez, Barbara Chávez Mazari, Alfonso Ponce de León, María de Lourdes García García, Manuel Palacios Martíne. ...
Journal of Clinical Microbiology
The purpose of this open and multicenter trial was to determine the usefulness of antibody detect... more The purpose of this open and multicenter trial was to determine the usefulness of antibody detection by enzyme-linked immunosorbent assay (ELISA) in gingival crevicular transudate (GCT), which was collected with an investigational device (Orasure; Epitope, Beaverton, Oreg.), for the diagnosis of human immunodeficiency virus type 1 (HIV-1) infection and to compare it with antibody detection in serum. There were two false-negative gingival samples, one from an HIV-1-positive asymptomatic individual and one from a patient with AIDS. HIV-1 antibodies were unexpectedly detected in both serum and GCT of two individuals, one with dental disease and one with pulmonary tuberculosis. None of the sera or GCTs from healthy subjects or patients with AD were positive. Compared with the serum assay, the sensitivity, specificity, and positive and negative predictive values of the GCT assay were 99.5, 100, 100, and 99.9%, respectively. Of 355 paired serum-GCT samples that were HIV-1 positive by ELISA and that
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
To describe the epidemiology and prognosis of P. aeruginosa bloodstream infections in a tertiary-... more To describe the epidemiology and prognosis of P. aeruginosa bloodstream infections in a tertiary-care center. Retrospective analysis. Tertiary-care teaching hospital in Mexico City. All cases of P. aeruginosa bacteremia diagnosed from 1981 to 1994. Relevant demographic, clinical and therapeutic variables were analyzed. A total of 153 bacteremias were found between 1981 and 1994, with a mean prevalence of 4.1 episodes per 1000 hospital discharges. Twenty-five percent of the infections derived from the biliary tract, and the most frequent underlying diseases were hematologic malignancies. The overall crude mortality was 46% (70/153) whereas mortality in the nosocomially-acquired episodes was 47% (58/124). Mortality within the first 72 h was 24% (37/153). A multivariate analysis showed six risk factors associated with a fatal outcome: age > or = 40 years, shock, mechanical ventilation, prior use of antibiotics, splenectomy and inappropriate selection of antibiotics. The identification of risk factors, and therefore a prompt instauration of specific antibiotic therapy, improved the prognosis of these severely ill patients.
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
To determine the clinical manifestations associated with resistant M. tuberculosis infection and ... more To determine the clinical manifestations associated with resistant M. tuberculosis infection and the antimicrobial resistance in isolates from Mexican patients. Epidemiological surveillance. Tuberculosis confirmed cases. Primary resistance: no history of treatment prior to diagnosis. The following critical concentrations (micrograms/mL) were used for susceptibility: isoniazid 0.2 and 1; rifampin 1 and 5; ethambutol 5 and 10; streptomycin 2 and 10; ethionamide 5; kanamycin 6; and para-aminosalicylic acid (PAS) 2 and 10. Eighty-four patients with a mean age of 44.7 years were included; 54 men (64%) and 30 women (36%); most patients were from the Mexico City metropolitan area. In 34 patients there was clinical information available, 26 presented fever and weight loss and 8 respiratory symptoms. Fifty-nine patients (70%) were infected by pan-susceptible M. tuberculosis, and 25 (30%) by a resistant isolate; 17 (68%) of them were resistant to at least two drugs, 16 (64%) to isoniazid and rifampin. The proportion of resistance was: isoniazid 24%, rifampin 19%, streptomycin 12%, ethambutol 10%, PAS 9%, etionamide 7%, and kanamycin 6%. Of 47 patients without previous treatment, eight had a resistant microorganism (17%): 9% resistant to isoniazid, 6% to rifampin, 2% to streptomycin, 6% to PAS and 6% multiresistant. Of 37 patients with history of previous treatment for tuberculosis, 17 (46%) had a resistant isolate; 44% were resistant to isoniazid, 35% to rifampin, 24% to streptomycin, 19% to ethambutol, 12% to PAS and 35% multiresistant. Of the 84 patients, four were physicians infected by a resistant isolate, and seven HIV-infected patients, one with a multiresistant isolate, and another with isoniazid resistance. Antimicrobial resistance among M. tuberculosis is alarmingly high in Mexico City; these results emphasize the importance of case detection and early isolation of patients.
JAMA The Journal of the American Medical Association
... in such settings remain an open question.2 Alfredo Ponce de Leon, MD Peter M. Small, MD Stanf... more ... in such settings remain an open question.2 Alfredo Ponce de Leon, MD Peter M. Small, MD Stanford University Medical Center Stanford, Calif Jose Sifuentes-Osornio, MD Instituto Nacional de la Nutricion Salvador Zubiran Mexico City, Mexico 1. Frieden TR, Sherman LF, Maw ...
Page 1. Otras secciones de este sitio: ...
Journal of Clinical Microbiology
The present paper analyzes the histoplasmin electrophoretic profiles and the randomly amplified p... more The present paper analyzes the histoplasmin electrophoretic profiles and the randomly amplified polymorphic DNA (RAPD) patterns of the fungus Histoplasma capsulatum isolated from Mexican patients with AIDSassociated histoplasmosis. Clinical isolates from Guatemala, Colombia, and Panama, as well as H. capsulatum isolates from different sources in nature, were also processed. All histoplasmin samples shared four antigenic fractions of 200, 49, 10.5, and 8.5 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). According to their percentage of relatedness, based on SDS-PAGE histoplasmin electrophoretic image analysis, H. capsulatum isolates were divided in two groups: group A contained all AIDS-associated isolates studied and two human reference strains from Mexican histoplasmosis patients without AIDS; group B included bat guano, infected bat, and cock excreta isolates from the State of Guerrero, Mexico, plus three human histoplasmosis strains from Guatemala, Panama, and Colombia. Polymorphic DNA patterns evaluated by RAPD-PCR showed three major bands of 4.4, 3.2, and 2.3 kb in most H. capsulatum isolates studied. Four groups were related by DNA polymorphisms: group I was formed by most of the AIDS-associated H. capsulatum isolates studied, one human histoplasmosis strain from Colombia, two human reference strains from Mexican patients without AIDS, and one human histoplasmosis strain from Guatemala. Group II consisted of only a single strain from Panama. Group III included three strains: one from a Mexican patient with AIDS and two isolated from nature in Guerrero (cock excreta and bat guano). The last, group IV, consisted of only one strain isolated from an infected bat, captured in Guerrero. A tight relationship between phenotypic and genotypic characterization was observed, and both analyses could be useful tools for typing H. capsulatum from different sources and geographic origins.
PLOS Neglected Tropical Diseases, 2015
Mycobacterium tuberculosis causes the majority of tuberculosis (TB) cases in humans; however, in ... more Mycobacterium tuberculosis causes the majority of tuberculosis (TB) cases in humans; however, in developing countries, human TB caused by M. bovis may be frequent but undetected. Human TB caused by M. bovis is considered a zoonosis; transmission is mainly through consumption of unpasteurized dairy products, and it is less frequently attributed to animal-to-human or human-to-human contact. We describe the trends of M. bovis isolation from human samples and first-line drug susceptibility during a 15-year period in a referral laboratory located in a tertiary care hospital in Mexico City.
RESUMEN Antecedentes. La tuberculosis, declarada como emergencia mundial por la Organización Mund... more RESUMEN Antecedentes. La tuberculosis, declarada como emergencia mundial por la Organización Mundial de la Salud es aún un problema importante de salud pública en México, incluida entre las primeras 20 causas de muerte. Objetivo. Conocer el impacto de ...
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion
García-García L, et al. Indoor pollution as risk factor for pulmonary TB. Rev Invest Clin 2009; 6... more García-García L, et al. Indoor pollution as risk factor for pulmonary TB. Rev Invest Clin 2009; 61 (5): 392-398 392 Revista de Investigación Clínica / Vol. 61, Núm. 5 / Septiembre-Octubre, 2009 / pp 392-398 Versión completa de este artículo disponible en internet: www.imbiomed.com.mx