Jose Naranjo Paramo - Academia.edu (original) (raw)

Papers by Jose Naranjo Paramo

Research paper thumbnail of PO21-643 Hypertriglyceridemic Waist is Associated With Markers of Inflammation in Asymptomatic Middle-Aged Men

Atherosclerosis Supplements, 2007

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Research paper thumbnail of Association between matrix metalloproteinase 2 (MMP2) promoter polymorphisms and the susceptibility to non-Hodgkin’s lymphoma in Egyptians

Annals of Hematology, 2014

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases capable of extracellular matri... more Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases capable of extracellular matrix degradation. MMP2 is the key molecule that control invasion, tumor growth, and metastasis, and has been associated with poor prognosis in several tumors. Several epidemiological studies have focused on the associations between MMP2 promoter polymorphisms and cancer susceptibility; however, little is known about their role in hematological malignancies. The present study aimed to investigate the association of MMP2 -735C/T and -1306C/T promoter polymorphisms with B-NHL susceptibility and their clinicopathological characteristics. The study included 100 B-NHL patients and 100 healthy controls. Genotyping of MMP2 -735C/T and MMP2 -1306C/T was done by polymerase chain reaction restricted fragment length polymorphism (PCR-RFLP) technique. MMP2 -735C/T heteromutant genotype (CT) was detected in 23 % of patients, and the homomutant genotype (TT) was detected in 7 % of patients. The polymorphic allele, T allele, was associated with susceptibility to B-NHL (OR = 2.8:95 %CI = 1.48-5.28). For MMP2 -1306C/T, the frequencies of the polymorphic variants were 5 % for the heteromutant genotype (CT) and 3 % for the homomutant genotype (TT). The polymorphic allele, T allele, conferred almost fourfold increased risk of B-NHL (OR = 3.8, 95 %CI = 1.05-13.9), and the risk elevated to be almost eight folds when confined to diffuse large B-cell lymphoma (DLBCL) (OR = 7.9, 95 %CI = 1.67-32.27). MMP2 -735C/T polymorphic genotypes were correlated with advanced clinical stages of the disease (stages III and IV). In conclusion, the study revealed that the variant alleles of MMP2 -735C/T and MMP2 -1306C/T can be considered as molecular risk factors for B-NHL among Egyptians.

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Research paper thumbnail of W05-O-003 Pravastatin reduces IL-1 induced endothelial PAI-1 expression in a genotype-dependent manner

Atherosclerosis Supplements, 2005

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Research paper thumbnail of Monocyte Cyclooxygenase-2 Activity: A New Therapeutic Target for Atherosclerosis?

Current Drug Target -Cardiovascular & Hematological Disorders, 2005

It is now widely accepted that atherosclerosis is a complex chronic inflammatory disorder of the ... more It is now widely accepted that atherosclerosis is a complex chronic inflammatory disorder of the arterial tree associated with several risk factors. From the initial phases of leukocyte recruitment to eventual rupture of vulnerable atherosclerotic plaques, a low-grade inflammation, also termed microinflammation, appears to play a key pathogenetic role. Experimental and clinical evidence suggests that cyclooxygenase-2 (COX-2), an enzyme which catalyzes the generation of prostaglandins from arachidonic acid, also contributes to lesion formation. COX-2 has been detected in macrophages, smooth muscle cells and endothelial cells in human atherosclerotic lesions. Several studies have also reported the presence of COX-2 in the shoulder region of atherosclerotic plaques, mainly colocalizing with macrophages and MMPs, enzymes that are involved in the destabilization of atherosclerotic plaques, leading to rupture and atherothrombotic syndromes (i.e. acute myocardial infarction). We have recently assessed monocyte COX-2 activity and the production of PGE(2) in a population of apparently healthy subjects free from clinically overt atherosclerosis. We found an association between increased PGE(2) and increasing number of cardiovascular risk factors and carotid intima-media thickness, a noninvasive surrogate marker of atherosclerosis, independently of traditional and non traditional cardiovascular risk factors. Our findings support the notion that the COX-2/PGE(2)axis may have a role in atherosclerosis, and this might be an attractive therapeutic target. COX-2 inhibitors, collectively called coxibs (celecoxib, rofecoxib, valdecoxib, lumiracoxib, etc), held a promise of improved treatment of arthritis without the gastrointestinal side effects associated with aspirin and other nonsteroidal anti-inflammatory drugs. However, clinical studies raise several clinically relevant questions as to their beneficial role in atherosclerosis prevention, because of increased thrombogenicity and cardiovascular risk. Only well designed large scale clinical trials can provide the answer as to the net effect of selective COX-2 inhibition on cardiovascular events before this new class of anti-inflammatory drugs can be incorporated into the armamentarium of atherosclerosis.

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Research paper thumbnail of Functional MMP-10 is required for efficient tissue repair after experimental hind limb ischemia

The FASEB Journal, 2014

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Research paper thumbnail of Radioembolization of hepatocellular carcinoma activates liver regeneration, induces inflammation and endothelial stress and activates coagulation

Liver International, 2014

Radioembolization may rarely induce liver disease resulting in a syndrome that is similar to veno... more Radioembolization may rarely induce liver disease resulting in a syndrome that is similar to veno-occlusive disease complicating bone marrow transplantation where inflammation, endothelial cell activation and thrombosis are likely involved. We hypothesized that similar mechanisms could be implicated in radioembolization-induced liver disease (REILD). Moreover, lobar radioembolization may induce hypertrophy of the non-treated hemiliver most probably by inducing liver regeneration. In patients with hepatocellular carcinoma, we prospectively studied serum levels of markers of liver regeneration, oxidative stress, pro-inflammatory pathways, endothelial activation and coagulation parameters over 2 months after radioembolization. Although REILD did not occur among 14 treated patients, a decrease in effective liver blood flow was observed. Radioembolization was followed by a persistent increase in pro-inflammatory (interleukin 6 and 8) and oxidative stress (malondyaldehide) markers, an induction of endothelial injury markers (vW factor and PAI-1) and an activation of the coagulation cascade (factor VIII, PAI-1, D-Dimer) as well as a significant increase in factors related to liver regeneration (FGF-19 and HGF). Radioembolization activates liver regeneration, produces oxidative stress, activates inflammatory cytokines and induces endothelial injury with partial activation of the coagulation cascade. These findings may have implications in the pathogenesis, prevention and therapy of REILD and in the development of new therapies to enhance hypertrophy with a surgical perspective.

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Research paper thumbnail of Hemostasis in Advanced Liver Disease

Seminars in Thrombosis and Hemostasis, 1993

1. Semin Thromb Hemost. 1993;19(3):184-90. Hemostasis in advanced liver disease. Páramo JA, Rocha... more 1. Semin Thromb Hemost. 1993;19(3):184-90. Hemostasis in advanced liver disease. Páramo JA, Rocha E. Hematology Service, University Hospital, School of Medicine, University of Navarra, Pamplona, Spain. PMID: 8362247 [PubMed - indexed for MEDLINE]. ...

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Research paper thumbnail of Effectiveness of Aprotinin in Orthotopic Liver Transplantation

Seminars in Thrombosis and Hemostasis, 1993

1. Semin Thromb Hemost. 1993;19(3):292-6. Effectiveness of aprotinin in orthotopic liver transpla... more 1. Semin Thromb Hemost. 1993;19(3):292-6. Effectiveness of aprotinin in orthotopic liver transplantation. Suárez M, Sangro B, Herrero JI, Picardi A, Páramo JA, Quiroga J, Pardo F, Cienfuegos J, Carrera J, Hidalgo F, et al. Liver Unit, Universidad de Navarra, Pamplona, Spain. ...

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Research paper thumbnail of A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction☆

Cardiovascular Research, 2006

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Research paper thumbnail of Vitamins C and E prevent endothelial VEGF and VEGFR-2 overexpression induced by porcine hypercholesterolemic LDL

Cardiovascular Research, 2005

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Research paper thumbnail of Fibrinogen Oviedo I. A new Spanish dysfibrinogenaemia

Blood Coagulation & Fibrinolysis, 1990

An abnormal fibrinogen was discovered in the plasma of a clinically asymptomatic woman. Laborator... more An abnormal fibrinogen was discovered in the plasma of a clinically asymptomatic woman. Laboratory evaluation of five members of the affected family showed low fibrinogen values in kinetic assays whereas the fibrinogen levels, tested by immunological procedures were normal. The patient's plasma had an inhibitory effect on the thrombin time of normal plasma. The calcium ions totally corrected the thrombin and reptilase times. Either low or high ionic strength prolonged the thrombin time of the proposita's purified fibrinogen. Kinetic analysis of clotting by monitoring transmission at 350 nm showed abnormally slow clotting with thrombin and reptilase. Assays were preformed in whole plasma as well as in purified fibrinogen. A delay in the rate of polymerization was evident when purified patient monomers were compared with those of normals. Immunoelectrophoretic, chromatofocusing, and isoelectrofusing experiments detected neither structural nor immunological abnormalities of fibrinogen. The rate of release of fibrinopeptide A by thrombin, measured by a specific immunoenzymatic method was also normal. HPLC analysis showed normal liberation of fibrinopeptides after prolonged thrombin action. Cross-linking of fibrin by factor XIII and lysis of fibrinogen by plasmin were normal. In view of these results, the defect of this dysfibrinogenemia, designated as Fibrinogen Oviedo I, probably could be due to conformational modifications in the D section of the molecule.

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Research paper thumbnail of W15.429 Independent association of prothrombin fragment 1+2 with carotid intima-media thickness in asymptomatic subjects

Atherosclerosis, 2004

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Research paper thumbnail of Effect of stocking density on growth, survival and cheliped injuries of stage 2 juvenile signal crayfish Pasifastacus leniusculus Dana

Aquaculture, 2004

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Research paper thumbnail of Effect of stocking density on growth, survival and yield of juvenile redclaw crayfish Cherax quadricarinatus (Decapoda: Parastacidae) in gravel-lined commercial nursery ponds

Studies evaluating the effect of stocking density on growth, survival and yield of redclaw crayfi... more Studies evaluating the effect of stocking density on growth, survival and yield of redclaw crayfish Cherax quadricarinatus have been limited to grow out stages. In this study, an experiment was conducted to assess such effects by stocking 5, 6, 8, 11 or 20 juveniles m À 2 in gravel-lined nursery ponds (2500 m 2 each) of a commercial farm in Ecuador, and to determine the feasibility of obtaining a minimum size of 25 g of crayfish after 80 days of nursery stage maintenance. Shelter to cover 50% of the initial population in each pond was provided. Fertilizer and a pelleted ration were used to stimulate growth of redclaw crayfish. There were significant differences in final weight, survival and yield among stocking densities. Significant inverse linear relationships were observed for final weight and survival, in relation to density level (P < 0.05, R 2 = 0.83 and R 2 = 0.74, respectively). Stocking at 11 specimens m À 2 (or lower rates) permitted the minimum size of 25 g to be obtained. Yield was directly related to density (P < 0.05, R 2 = 0.93). No evidence for feed conversion ratio being affected by density was found. Growth varied during the trial and the estimate of a single, overall instantaneous growth rate or a specific growth rate, using the exponential equation, was inadequate. A model proposed in this study allowed a satisfactory description for comparing growth curves among treatments. The model exhibited desirable, close-to-linear estimation properties of parameters, and invariance tests showed that density significantly affected values of its parameters (P < 0.05). Uniform and acceptable water quality conditions in all treatments indicated that the effect of stocking density in production of crayfish was most probably exerted by non-aggressive interactions or behavioral factors of the individuals. Results of the experimental trial were acceptable 0044-8486/$-see front matter D

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Research paper thumbnail of PO21-643 Hypertriglyceridemic Waist is Associated With Markers of Inflammation in Asymptomatic Middle-Aged Men

Atherosclerosis Supplements, 2007

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Association between matrix metalloproteinase 2 (MMP2) promoter polymorphisms and the susceptibility to non-Hodgkin’s lymphoma in Egyptians

Annals of Hematology, 2014

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases capable of extracellular matri... more Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases capable of extracellular matrix degradation. MMP2 is the key molecule that control invasion, tumor growth, and metastasis, and has been associated with poor prognosis in several tumors. Several epidemiological studies have focused on the associations between MMP2 promoter polymorphisms and cancer susceptibility; however, little is known about their role in hematological malignancies. The present study aimed to investigate the association of MMP2 -735C/T and -1306C/T promoter polymorphisms with B-NHL susceptibility and their clinicopathological characteristics. The study included 100 B-NHL patients and 100 healthy controls. Genotyping of MMP2 -735C/T and MMP2 -1306C/T was done by polymerase chain reaction restricted fragment length polymorphism (PCR-RFLP) technique. MMP2 -735C/T heteromutant genotype (CT) was detected in 23 % of patients, and the homomutant genotype (TT) was detected in 7 % of patients. The polymorphic allele, T allele, was associated with susceptibility to B-NHL (OR = 2.8:95 %CI = 1.48-5.28). For MMP2 -1306C/T, the frequencies of the polymorphic variants were 5 % for the heteromutant genotype (CT) and 3 % for the homomutant genotype (TT). The polymorphic allele, T allele, conferred almost fourfold increased risk of B-NHL (OR = 3.8, 95 %CI = 1.05-13.9), and the risk elevated to be almost eight folds when confined to diffuse large B-cell lymphoma (DLBCL) (OR = 7.9, 95 %CI = 1.67-32.27). MMP2 -735C/T polymorphic genotypes were correlated with advanced clinical stages of the disease (stages III and IV). In conclusion, the study revealed that the variant alleles of MMP2 -735C/T and MMP2 -1306C/T can be considered as molecular risk factors for B-NHL among Egyptians.

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Research paper thumbnail of W05-O-003 Pravastatin reduces IL-1 induced endothelial PAI-1 expression in a genotype-dependent manner

Atherosclerosis Supplements, 2005

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Research paper thumbnail of Monocyte Cyclooxygenase-2 Activity: A New Therapeutic Target for Atherosclerosis?

Current Drug Target -Cardiovascular & Hematological Disorders, 2005

It is now widely accepted that atherosclerosis is a complex chronic inflammatory disorder of the ... more It is now widely accepted that atherosclerosis is a complex chronic inflammatory disorder of the arterial tree associated with several risk factors. From the initial phases of leukocyte recruitment to eventual rupture of vulnerable atherosclerotic plaques, a low-grade inflammation, also termed microinflammation, appears to play a key pathogenetic role. Experimental and clinical evidence suggests that cyclooxygenase-2 (COX-2), an enzyme which catalyzes the generation of prostaglandins from arachidonic acid, also contributes to lesion formation. COX-2 has been detected in macrophages, smooth muscle cells and endothelial cells in human atherosclerotic lesions. Several studies have also reported the presence of COX-2 in the shoulder region of atherosclerotic plaques, mainly colocalizing with macrophages and MMPs, enzymes that are involved in the destabilization of atherosclerotic plaques, leading to rupture and atherothrombotic syndromes (i.e. acute myocardial infarction). We have recently assessed monocyte COX-2 activity and the production of PGE(2) in a population of apparently healthy subjects free from clinically overt atherosclerosis. We found an association between increased PGE(2) and increasing number of cardiovascular risk factors and carotid intima-media thickness, a noninvasive surrogate marker of atherosclerosis, independently of traditional and non traditional cardiovascular risk factors. Our findings support the notion that the COX-2/PGE(2)axis may have a role in atherosclerosis, and this might be an attractive therapeutic target. COX-2 inhibitors, collectively called coxibs (celecoxib, rofecoxib, valdecoxib, lumiracoxib, etc), held a promise of improved treatment of arthritis without the gastrointestinal side effects associated with aspirin and other nonsteroidal anti-inflammatory drugs. However, clinical studies raise several clinically relevant questions as to their beneficial role in atherosclerosis prevention, because of increased thrombogenicity and cardiovascular risk. Only well designed large scale clinical trials can provide the answer as to the net effect of selective COX-2 inhibition on cardiovascular events before this new class of anti-inflammatory drugs can be incorporated into the armamentarium of atherosclerosis.

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Research paper thumbnail of Functional MMP-10 is required for efficient tissue repair after experimental hind limb ischemia

The FASEB Journal, 2014

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Research paper thumbnail of Radioembolization of hepatocellular carcinoma activates liver regeneration, induces inflammation and endothelial stress and activates coagulation

Liver International, 2014

Radioembolization may rarely induce liver disease resulting in a syndrome that is similar to veno... more Radioembolization may rarely induce liver disease resulting in a syndrome that is similar to veno-occlusive disease complicating bone marrow transplantation where inflammation, endothelial cell activation and thrombosis are likely involved. We hypothesized that similar mechanisms could be implicated in radioembolization-induced liver disease (REILD). Moreover, lobar radioembolization may induce hypertrophy of the non-treated hemiliver most probably by inducing liver regeneration. In patients with hepatocellular carcinoma, we prospectively studied serum levels of markers of liver regeneration, oxidative stress, pro-inflammatory pathways, endothelial activation and coagulation parameters over 2 months after radioembolization. Although REILD did not occur among 14 treated patients, a decrease in effective liver blood flow was observed. Radioembolization was followed by a persistent increase in pro-inflammatory (interleukin 6 and 8) and oxidative stress (malondyaldehide) markers, an induction of endothelial injury markers (vW factor and PAI-1) and an activation of the coagulation cascade (factor VIII, PAI-1, D-Dimer) as well as a significant increase in factors related to liver regeneration (FGF-19 and HGF). Radioembolization activates liver regeneration, produces oxidative stress, activates inflammatory cytokines and induces endothelial injury with partial activation of the coagulation cascade. These findings may have implications in the pathogenesis, prevention and therapy of REILD and in the development of new therapies to enhance hypertrophy with a surgical perspective.

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Research paper thumbnail of Hemostasis in Advanced Liver Disease

Seminars in Thrombosis and Hemostasis, 1993

1. Semin Thromb Hemost. 1993;19(3):184-90. Hemostasis in advanced liver disease. Páramo JA, Rocha... more 1. Semin Thromb Hemost. 1993;19(3):184-90. Hemostasis in advanced liver disease. Páramo JA, Rocha E. Hematology Service, University Hospital, School of Medicine, University of Navarra, Pamplona, Spain. PMID: 8362247 [PubMed - indexed for MEDLINE]. ...

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Research paper thumbnail of Effectiveness of Aprotinin in Orthotopic Liver Transplantation

Seminars in Thrombosis and Hemostasis, 1993

1. Semin Thromb Hemost. 1993;19(3):292-6. Effectiveness of aprotinin in orthotopic liver transpla... more 1. Semin Thromb Hemost. 1993;19(3):292-6. Effectiveness of aprotinin in orthotopic liver transplantation. Suárez M, Sangro B, Herrero JI, Picardi A, Páramo JA, Quiroga J, Pardo F, Cienfuegos J, Carrera J, Hidalgo F, et al. Liver Unit, Universidad de Navarra, Pamplona, Spain. ...

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Research paper thumbnail of A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction☆

Cardiovascular Research, 2006

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Research paper thumbnail of Vitamins C and E prevent endothelial VEGF and VEGFR-2 overexpression induced by porcine hypercholesterolemic LDL

Cardiovascular Research, 2005

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Fibrinogen Oviedo I. A new Spanish dysfibrinogenaemia

Blood Coagulation & Fibrinolysis, 1990

An abnormal fibrinogen was discovered in the plasma of a clinically asymptomatic woman. Laborator... more An abnormal fibrinogen was discovered in the plasma of a clinically asymptomatic woman. Laboratory evaluation of five members of the affected family showed low fibrinogen values in kinetic assays whereas the fibrinogen levels, tested by immunological procedures were normal. The patient&amp;amp;#39;s plasma had an inhibitory effect on the thrombin time of normal plasma. The calcium ions totally corrected the thrombin and reptilase times. Either low or high ionic strength prolonged the thrombin time of the proposita&amp;amp;#39;s purified fibrinogen. Kinetic analysis of clotting by monitoring transmission at 350 nm showed abnormally slow clotting with thrombin and reptilase. Assays were preformed in whole plasma as well as in purified fibrinogen. A delay in the rate of polymerization was evident when purified patient monomers were compared with those of normals. Immunoelectrophoretic, chromatofocusing, and isoelectrofusing experiments detected neither structural nor immunological abnormalities of fibrinogen. The rate of release of fibrinopeptide A by thrombin, measured by a specific immunoenzymatic method was also normal. HPLC analysis showed normal liberation of fibrinopeptides after prolonged thrombin action. Cross-linking of fibrin by factor XIII and lysis of fibrinogen by plasmin were normal. In view of these results, the defect of this dysfibrinogenemia, designated as Fibrinogen Oviedo I, probably could be due to conformational modifications in the D section of the molecule.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of W15.429 Independent association of prothrombin fragment 1+2 with carotid intima-media thickness in asymptomatic subjects

Atherosclerosis, 2004

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect of stocking density on growth, survival and cheliped injuries of stage 2 juvenile signal crayfish Pasifastacus leniusculus Dana

Aquaculture, 2004

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Effect of stocking density on growth, survival and yield of juvenile redclaw crayfish Cherax quadricarinatus (Decapoda: Parastacidae) in gravel-lined commercial nursery ponds

Studies evaluating the effect of stocking density on growth, survival and yield of redclaw crayfi... more Studies evaluating the effect of stocking density on growth, survival and yield of redclaw crayfish Cherax quadricarinatus have been limited to grow out stages. In this study, an experiment was conducted to assess such effects by stocking 5, 6, 8, 11 or 20 juveniles m À 2 in gravel-lined nursery ponds (2500 m 2 each) of a commercial farm in Ecuador, and to determine the feasibility of obtaining a minimum size of 25 g of crayfish after 80 days of nursery stage maintenance. Shelter to cover 50% of the initial population in each pond was provided. Fertilizer and a pelleted ration were used to stimulate growth of redclaw crayfish. There were significant differences in final weight, survival and yield among stocking densities. Significant inverse linear relationships were observed for final weight and survival, in relation to density level (P < 0.05, R 2 = 0.83 and R 2 = 0.74, respectively). Stocking at 11 specimens m À 2 (or lower rates) permitted the minimum size of 25 g to be obtained. Yield was directly related to density (P < 0.05, R 2 = 0.93). No evidence for feed conversion ratio being affected by density was found. Growth varied during the trial and the estimate of a single, overall instantaneous growth rate or a specific growth rate, using the exponential equation, was inadequate. A model proposed in this study allowed a satisfactory description for comparing growth curves among treatments. The model exhibited desirable, close-to-linear estimation properties of parameters, and invariance tests showed that density significantly affected values of its parameters (P < 0.05). Uniform and acceptable water quality conditions in all treatments indicated that the effect of stocking density in production of crayfish was most probably exerted by non-aggressive interactions or behavioral factors of the individuals. Results of the experimental trial were acceptable 0044-8486/$-see front matter D

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