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Papers by Josep Corominas
FEBS Letters, 1992
Disruption of the gene pgil or Srrcckrrom~ccs cerruisiae, which codes for phosphoglucose isomcras... more Disruption of the gene pgil or Srrcckrrom~ccs cerruisiae, which codes for phosphoglucose isomcrase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6pbosphau was much higher in mutant than in wild-type cells. Phosphorylase R activity and the state of activation of glycogen synthuse were also inveslipted. Phosphorylase a activity was rather low along the culture in wild-type cells, whereas it was consistently higher in mutants. Glycogen synthasc was mostly in the active form in early-medium exponential cultures in wild-type cells whereas the activation state of this enzyme in mutant cells, although lower at the earlier steps of the culture, did not direr from wild-type cells at later slag&. The fact that the intracellular lcvcls of UDP-glucose are markedly Incrensed in mutant cells sug&st that the observed accumulation of giycogen results from a rise in substrate availability rather than from the activation of the enzyme responsible for the synthesis of the polysaccharide.
FEBS Letters, 1992
Disruption of the gene pgil or Srrcckrrom~ccs cerruisiae, which codes for phosphoglucose isomcras... more Disruption of the gene pgil or Srrcckrrom~ccs cerruisiae, which codes for phosphoglucose isomcrase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6pbosphau was much higher in mutant than in wild-type cells. Phosphorylase R activity and the state of activation of glycogen synthuse were also inveslipted. Phosphorylase a activity was rather low along the culture in wild-type cells, whereas it was consistently higher in mutants. Glycogen synthasc was mostly in the active form in early-medium exponential cultures in wild-type cells whereas the activation state of this enzyme in mutant cells, although lower at the earlier steps of the culture, did not direr from wild-type cells at later slag&. The fact that the intracellular lcvcls of UDP-glucose are markedly Incrensed in mutant cells sug&st that the observed accumulation of giycogen results from a rise in substrate availability rather than from the activation of the enzyme responsible for the synthesis of the polysaccharide.