Józef Adamczyk - Academia.edu (original) (raw)
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Papers by Józef Adamczyk
BMC Genomics, 2016
Recent progress in selective breeding of maize (Zea mays L.) towards adaptation to temperate clim... more Recent progress in selective breeding of maize (Zea mays L.) towards adaptation to temperate climate has allowed the production of inbred lines withstanding cold springs with temperatures below 8 °C or even close to 0 °C, indicating that despite its tropical origins maize is not inherently cold-sensitive. Here we studied the acclimatory response of three maize inbred lines of contrasting cold-sensitivity selected basing on multi-year routine field data. The field observations were confirmed in the growth chamber. Under controlled conditions the damage to the photosynthetic apparatus due to severe cold treatment was the least in the cold-tolerant line provided that it had been subjected to prior moderate chilling, i.e., acclimation. The cold-sensitive lines performed equally poorly with or without acclimation. To uncover the molecular basis of the attained cold-acclimatability we performed comparative transcriptome profiling of the response of the lines to the cold during acclimation phase by means of microarrays with a statistical and bioinformatic data analysis. The analyses indicated three mechanisms likely responsible for the cold-tolerance: acclimation-dependent modification of the photosynthetic apparatus, cell wall properties, and developmental processes. Those conclusions supported the observed acclimation of photosynthesis to severe cold at moderate chilling and were further confirmed by experimentally showing specific modification of cell wall properties and repression of selected miRNA species, general regulators of development, in the cold-tolerant line subjected to cold stress.
Journal of Plant Physiology, 2005
In order to investigate the mechanisms of maize adaptation to temperate climate, we studied photo... more In order to investigate the mechanisms of maize adaptation to temperate climate, we studied photosynthetic efficiency, as evaluated by means of F PSII and chloroplast ultrastructure, as well as growth and development of two inbred lines (the chillingtolerant KW 1074 and the chilling-sensitive CM 109) under laboratory conditions. Plants were grown from seed to the 3rd leaf stage at a suboptimal temperature (14 1C/ 12 1C) and then the temperature was increased to 24 1C/22 1C. To verify the results obtained with the two model lines, twelve inbred lines were tested under both laboratory and field conditions. .pl (P. Sowiński).
BMC Genomics, 2016
Recent progress in selective breeding of maize (Zea mays L.) towards adaptation to temperate clim... more Recent progress in selective breeding of maize (Zea mays L.) towards adaptation to temperate climate has allowed the production of inbred lines withstanding cold springs with temperatures below 8 °C or even close to 0 °C, indicating that despite its tropical origins maize is not inherently cold-sensitive. Here we studied the acclimatory response of three maize inbred lines of contrasting cold-sensitivity selected basing on multi-year routine field data. The field observations were confirmed in the growth chamber. Under controlled conditions the damage to the photosynthetic apparatus due to severe cold treatment was the least in the cold-tolerant line provided that it had been subjected to prior moderate chilling, i.e., acclimation. The cold-sensitive lines performed equally poorly with or without acclimation. To uncover the molecular basis of the attained cold-acclimatability we performed comparative transcriptome profiling of the response of the lines to the cold during acclimation phase by means of microarrays with a statistical and bioinformatic data analysis. The analyses indicated three mechanisms likely responsible for the cold-tolerance: acclimation-dependent modification of the photosynthetic apparatus, cell wall properties, and developmental processes. Those conclusions supported the observed acclimation of photosynthesis to severe cold at moderate chilling and were further confirmed by experimentally showing specific modification of cell wall properties and repression of selected miRNA species, general regulators of development, in the cold-tolerant line subjected to cold stress.
Journal of Plant Physiology, 2005
In order to investigate the mechanisms of maize adaptation to temperate climate, we studied photo... more In order to investigate the mechanisms of maize adaptation to temperate climate, we studied photosynthetic efficiency, as evaluated by means of F PSII and chloroplast ultrastructure, as well as growth and development of two inbred lines (the chillingtolerant KW 1074 and the chilling-sensitive CM 109) under laboratory conditions. Plants were grown from seed to the 3rd leaf stage at a suboptimal temperature (14 1C/ 12 1C) and then the temperature was increased to 24 1C/22 1C. To verify the results obtained with the two model lines, twelve inbred lines were tested under both laboratory and field conditions. .pl (P. Sowiński).