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Papers by Juan Antonio Morales Sanchez

PLoS ONE, 2014

The long-term goal of our study is to understand the internal organization of the octocoral stem ... more The long-term goal of our study is to understand the internal organization of the octocoral stem canals, as well as their physiological and functional role in the growth of the colonies, and finally to assess the influence of climatic changes on this species. Here we focus on imaging tools, namely acquisition and processing of three-dimensional high-resolution images, with emphasis on automated extraction of canal pathways. Our aim was to evaluate the feasibility of the whole process, to point out and solve-if possible-technical problems related to the specimen conditioning, to determine the best acquisition parameters and to develop necessary image-processing algorithms. The pathways extracted are expected to facilitate the structural analysis of the colonies, namely to help observing the distribution, formation and number of canals along the colony. Five volumetric images of Muricea muricata specimens were successfully acquired by X-ray computed tomography with spatial resolution ranging from 4.5 to 25 micrometers. The success mainly depended on specimen immobilization. More than 85% of the canals were successfully detected and tracked by the image-processing method developed. Thus obtained three-dimensional representation of the canal network was generated for the first time without the need of histological or other destructive methods. Several canal patterns were observed. Although most of them were simple, i.e. only followed the main branch or ''turned'' into a secondary branch, many others bifurcated or fused. A majority of bifurcations were observed at branching points. However, some canals appeared and/or ended anywhere along a branch. At the tip of a branch, all canals fused into a unique chamber. Three-dimensional high-resolution tomographic imaging gives a non-destructive insight to the coral ultrastructure and helps understanding the organization of the canal network. Advanced image-processing techniques greatly reduce human observer's effort and provide methods to both visualize and quantify the structures of interest.

Journal of Fluid Mechanics, 2008

Accurate numerical computations of the onset of thermal convection in wide rotating spherical she... more Accurate numerical computations of the onset of thermal convection in wide rotating spherical shells are presented. Low-Prandtl-number (σ) fluids, and non-slip boundary conditions are considered. It is shown that at small Ekman numbers (E), and very low σ values, the well-known equatorially trapped patterns of convection are superseded by multicellular outer-equatorially-attached modes. As a result, the convection spreads to higher latitudes affecting the body of the fluid, and increasing the internal viscous dissipation. Then, from E < 10−5, the critical Rayleigh number (Rc) fulfils a power-law dependence Rc ~ E−4/3, as happens for moderate and high Prandtl numbers. However, the critical precession frequency (|ωc|) and the critical azimuthal wavenumber (mc) increase discontinuously, jumping when there is a change of the radial and latitudinal structure of the preferred eigenfunction. In addition, the transition between spiralling columnar (SC), and outer-equatorially-attached (O...

Circulation, 2012

Little is known about the pathophysiology of myxomatous degeneration of the mitral valve, the pat... more Little is known about the pathophysiology of myxomatous degeneration of the mitral valve, the pathological hallmark of mitral valve prolapse, associated with symptomatic mitral regurgitation, heart failure, and death. Excess transforming growth factor (TGF)-β signaling is known to cause mitral valve degeneration and regurgitation in a mouse model of Marfan syndrome. We examined if TGF-β signaling is dysregulated in clinical specimens of sporadic mitral valve prolapse compared with explanted nondiseased mitral valves and we tested the effects of angiotensin II receptor blockers on TGF-β signaling in cultured human mitral valve cells. Operative specimens, cultured valve tissues, and cultured valvular interstitial cells were obtained from patients with mitral valve prolapse undergoing mitral valve repair or from organ donors without mitral valve disease. Increased extracellular matrix in diseased valve tissue correlated with an upregulation of TGF-β expression and signaling as evidence...

Electron Devices, IEEE …, 2003

Abstract—The simulation of generation-recombination (GR) noise under periodic large-signal condit... more Abstract—The simulation of generation-recombination (GR) noise under periodic large-signal conditions in a partial differen-tial equation-based silicon device simulator is presented. Using the impedance-field method with cyclostationary noise sources, it is possible to simulate ...

International Journal of Computer Assisted Radiology and Surgery, 2011

Purpose Minimally Invasive Surgery (MIS) is a widely used surgical technique that requires a lon... more Purpose Minimally Invasive Surgery (MIS) is a widely used surgical technique that requires a long training process due to its difficulty and complexity. We developed an Augmented Reality Haptic (ARH) System based on electromagnetic tracking devices for use in creation training models (computer-enhanced trainers), in computer-assisted surgery or telemanipulation applications. Method The ARH system consists currently in a Linux driver and a

PLoS ONE, 2014

The long-term goal of our study is to understand the internal organization of the octocoral stem ... more The long-term goal of our study is to understand the internal organization of the octocoral stem canals, as well as their physiological and functional role in the growth of the colonies, and finally to assess the influence of climatic changes on this species. Here we focus on imaging tools, namely acquisition and processing of three-dimensional high-resolution images, with emphasis on automated extraction of canal pathways. Our aim was to evaluate the feasibility of the whole process, to point out and solve-if possible-technical problems related to the specimen conditioning, to determine the best acquisition parameters and to develop necessary image-processing algorithms. The pathways extracted are expected to facilitate the structural analysis of the colonies, namely to help observing the distribution, formation and number of canals along the colony. Five volumetric images of Muricea muricata specimens were successfully acquired by X-ray computed tomography with spatial resolution ranging from 4.5 to 25 micrometers. The success mainly depended on specimen immobilization. More than 85% of the canals were successfully detected and tracked by the image-processing method developed. Thus obtained three-dimensional representation of the canal network was generated for the first time without the need of histological or other destructive methods. Several canal patterns were observed. Although most of them were simple, i.e. only followed the main branch or ''turned'' into a secondary branch, many others bifurcated or fused. A majority of bifurcations were observed at branching points. However, some canals appeared and/or ended anywhere along a branch. At the tip of a branch, all canals fused into a unique chamber. Three-dimensional high-resolution tomographic imaging gives a non-destructive insight to the coral ultrastructure and helps understanding the organization of the canal network. Advanced image-processing techniques greatly reduce human observer's effort and provide methods to both visualize and quantify the structures of interest.

Journal of Fluid Mechanics, 2008

Accurate numerical computations of the onset of thermal convection in wide rotating spherical she... more Accurate numerical computations of the onset of thermal convection in wide rotating spherical shells are presented. Low-Prandtl-number (σ) fluids, and non-slip boundary conditions are considered. It is shown that at small Ekman numbers (E), and very low σ values, the well-known equatorially trapped patterns of convection are superseded by multicellular outer-equatorially-attached modes. As a result, the convection spreads to higher latitudes affecting the body of the fluid, and increasing the internal viscous dissipation. Then, from E < 10−5, the critical Rayleigh number (Rc) fulfils a power-law dependence Rc ~ E−4/3, as happens for moderate and high Prandtl numbers. However, the critical precession frequency (|ωc|) and the critical azimuthal wavenumber (mc) increase discontinuously, jumping when there is a change of the radial and latitudinal structure of the preferred eigenfunction. In addition, the transition between spiralling columnar (SC), and outer-equatorially-attached (O...

Circulation, 2012

Little is known about the pathophysiology of myxomatous degeneration of the mitral valve, the pat... more Little is known about the pathophysiology of myxomatous degeneration of the mitral valve, the pathological hallmark of mitral valve prolapse, associated with symptomatic mitral regurgitation, heart failure, and death. Excess transforming growth factor (TGF)-β signaling is known to cause mitral valve degeneration and regurgitation in a mouse model of Marfan syndrome. We examined if TGF-β signaling is dysregulated in clinical specimens of sporadic mitral valve prolapse compared with explanted nondiseased mitral valves and we tested the effects of angiotensin II receptor blockers on TGF-β signaling in cultured human mitral valve cells. Operative specimens, cultured valve tissues, and cultured valvular interstitial cells were obtained from patients with mitral valve prolapse undergoing mitral valve repair or from organ donors without mitral valve disease. Increased extracellular matrix in diseased valve tissue correlated with an upregulation of TGF-β expression and signaling as evidence...

Electron Devices, IEEE …, 2003

Abstract—The simulation of generation-recombination (GR) noise under periodic large-signal condit... more Abstract—The simulation of generation-recombination (GR) noise under periodic large-signal conditions in a partial differen-tial equation-based silicon device simulator is presented. Using the impedance-field method with cyclostationary noise sources, it is possible to simulate ...

International Journal of Computer Assisted Radiology and Surgery, 2011

Purpose Minimally Invasive Surgery (MIS) is a widely used surgical technique that requires a lon... more Purpose Minimally Invasive Surgery (MIS) is a widely used surgical technique that requires a long training process due to its difficulty and complexity. We developed an Augmented Reality Haptic (ARH) System based on electromagnetic tracking devices for use in creation training models (computer-enhanced trainers), in computer-assisted surgery or telemanipulation applications. Method The ARH system consists currently in a Linux driver and a