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Juan M Bueno

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Papers by Juan M Bueno

Research paper thumbnail of Biomimetic Remineralization of an Extracellular Matrix Collagen Membrane for Bone Regeneration

Polymers, Aug 11, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Dental Age Estimation Using Multiphoton Microscopy: A Potential Tool for Forensic Science

BioMed Research International, Jul 29, 2022

Normal aging affects the different structures of teeth, in particular, the dentine. These changes... more Normal aging affects the different structures of teeth, in particular, the dentine. These changes are useful in forensic disciplines as a tool for age estimation. Although multiphoton (MP) microscopy has been used to explore dental pieces, a relationship between age and MP response of the human dentine has not been proposed yet. The relationship between MP signals and natural dentine aging is investigated herein. An index of age (INAG) combining two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) images has been used to quantify these changes. The results show that the INAG significantly decreases with age. Moreover, peritubular dentine size and collagen internal properties are also modified with age. This information confirms the usefulness of this technique in forensic age estimation after disasters (natural or manmade) with a lack of comprehensive fingerprint database. Courts and other government authorities might also benefit from this tool when the official age of individuals under special circumstances is required for legal or medical reasons.

Research paper thumbnail of Retinal and Choroidal Thickness in Myopic Young Adults

Photonics

The retinal and the choroidal thickness were measured at four locations along the horizontal dire... more The retinal and the choroidal thickness were measured at four locations along the horizontal direction (foveola, one nasal to the fovea and two temporal) in a group of 43 young adults (mean age: 27.1 ± 3.9 years), with ocular refraction ranging from emmetropia to high myopia (0 to −10D). Thickness values were obtained from OCT images centered at the foveal depression. The retinal thickness exhibited a correlation with refraction at all eccentricities but not at the fovea. When different subgroups of refraction were considered, the analysis of such correlations indicated that only the retinal thickness in the group of high myopia (refraction ≤ −6D) was statistically different from the other two groups (emmetropes: [−0.5, 0] D, and myopes: (−6, −0.5) D). No significant differences were found between emmetropic and myopic groups. In contrast to the retina, the choroidal thickness exhibited a significant correlation with refraction at the fovea, although such dependency only stood for h...

Research paper thumbnail of Corneal and Total Aberrations from Childhood to Adulthood

Investigative Ophthalmology & Visual Science, 2003

Research paper thumbnail of Two-photon Microscopy to Visualize Amyloid Plaques in Unstained Retinas from Alzheimer’s Disease

Investigative Ophthalmology & Visual Science, Jun 11, 2015

Research paper thumbnail of Impact of the Retinal Reflection on the Wide-Angle Point Spread Function of the Human Eye

Investigative Ophthalmology & Visual Science, Jun 16, 2013

Research paper thumbnail of Retinal Cell Density In Chicks After Deprivation Myopia Measured With Adaptive-Optics Multiphoton Microscopy

Investigative Ophthalmology & Visual Science, Mar 26, 2012

Research paper thumbnail of Purkinje Imaging System to Objectively Measure Corneal Haze and Lenticular Scattering

Investigative Ophthalmology & Visual Science, May 10, 2007

Research paper thumbnail of Subjective Evaluation of Polarisation Images of the Optic Nerve Head and Retinal Structures

Investigative Ophthalmology & Visual Science, 2006

Research paper thumbnail of Improvement Of Confocal Scanning Laser ophthalmoscope Images Using Polarimetry

Investigative Ophthalmology & Visual Science, 2002

Research paper thumbnail of Collagen Crosslinking By Infrared Femtosecond Pulses In Ex-vivo Corneas

Investigative Ophthalmology & Visual Science, 2013

Research paper thumbnail of Two-photon Microscopy to Visualize Amyloid Plaques in Unstained Retinas from Alzheimer’s Disease

Investigative Ophthalmology & Visual Science, 2015

Research paper thumbnail of Quantifying the Quality of Confocal Scanning Laser Ophthalmoscopic Images of Blood Vessels

Investigative Ophthalmology & Visual Science, 2007

Research paper thumbnail of Interferometic and Hartmann-Shack Measurements of Aberrations in ex-vivo Human Crystalline Lenses

Investigative Ophthalmology & Visual Science, 2009

Research paper thumbnail of Polarimetry of the Optic Nerve Head and Lamina Cribrosa

Investigative Ophthalmology & Visual Science, 2004

Research paper thumbnail of Modeling Aberrations From Surface Measurements of ex-vivo Isolated Human Crystalline Lenses

Investigative Ophthalmology & Visual Science, 2009

Research paper thumbnail of Towards a background-free neutrinoless double beta decay experiment based on a fluorescent bicolor sensor

arXiv: Instrumentation and Detectors, 2019

Neutrinoless double beta decay ($\beta\beta0\nu$) is a putative nuclear decay that can occur if, ... more Neutrinoless double beta decay ($\beta\beta0\nu$) is a putative nuclear decay that can occur if, and only if, neutrinos are their own antiparticles. Due to the smallness of neutrino masses, the lifetime of betabeta0nu\beta\beta0\nubetabeta0nu is expected to be larger than 102610^{26}1026 yr, and the tiny expected signals are deeply buried in backgrounds associated with the natural radioactive chains, whose characteristic lifetime is sixteen orders of magnitude faster. Future experiments aiming to explore lifetimes several orders of magnitude larger will require the development of new experimental techniques to suppress those backgrounds. Since no known background processes converts xenon to barium, detection of the daughter ion in candidate decay events effectively eliminates backgrounds. It has been recently proposed that a xenon gas time projection chamber could unambiguously tag the betabeta0nu\beta\beta0\nubetabeta0nu decay Xe136rightarrowBa2++2e−(+2nu)Xe^{136} \rightarrow Ba^{2+} + 2e^- (+2 \nu)Xe136rightarrowBa2++2e(+2nu) by detecting the resulting Ba2+Ba^{2+}Ba2+ ion in a single-a...

Research paper thumbnail of Metrics to Quantify the Global Quality of Confocal Scanning Laser Ophthalmoscopic Images of the Aging Eye

Investigative Ophthalmology & Visual Science, 2007

Research paper thumbnail of Novel Scanning Characterization Approaches for the Accurate Understanding and Successful Treatment of Oral and Maxillofacial Pathologies

Research paper thumbnail of Adaptive Optics Multiphoton Microscopy of ex-vivo Ocular Tissues

Research paper thumbnail of Biomimetic Remineralization of an Extracellular Matrix Collagen Membrane for Bone Regeneration

Polymers, Aug 11, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Research paper thumbnail of Dental Age Estimation Using Multiphoton Microscopy: A Potential Tool for Forensic Science

BioMed Research International, Jul 29, 2022

Normal aging affects the different structures of teeth, in particular, the dentine. These changes... more Normal aging affects the different structures of teeth, in particular, the dentine. These changes are useful in forensic disciplines as a tool for age estimation. Although multiphoton (MP) microscopy has been used to explore dental pieces, a relationship between age and MP response of the human dentine has not been proposed yet. The relationship between MP signals and natural dentine aging is investigated herein. An index of age (INAG) combining two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) images has been used to quantify these changes. The results show that the INAG significantly decreases with age. Moreover, peritubular dentine size and collagen internal properties are also modified with age. This information confirms the usefulness of this technique in forensic age estimation after disasters (natural or manmade) with a lack of comprehensive fingerprint database. Courts and other government authorities might also benefit from this tool when the official age of individuals under special circumstances is required for legal or medical reasons.

Research paper thumbnail of Retinal and Choroidal Thickness in Myopic Young Adults

Photonics

The retinal and the choroidal thickness were measured at four locations along the horizontal dire... more The retinal and the choroidal thickness were measured at four locations along the horizontal direction (foveola, one nasal to the fovea and two temporal) in a group of 43 young adults (mean age: 27.1 ± 3.9 years), with ocular refraction ranging from emmetropia to high myopia (0 to −10D). Thickness values were obtained from OCT images centered at the foveal depression. The retinal thickness exhibited a correlation with refraction at all eccentricities but not at the fovea. When different subgroups of refraction were considered, the analysis of such correlations indicated that only the retinal thickness in the group of high myopia (refraction ≤ −6D) was statistically different from the other two groups (emmetropes: [−0.5, 0] D, and myopes: (−6, −0.5) D). No significant differences were found between emmetropic and myopic groups. In contrast to the retina, the choroidal thickness exhibited a significant correlation with refraction at the fovea, although such dependency only stood for h...

Research paper thumbnail of Corneal and Total Aberrations from Childhood to Adulthood

Investigative Ophthalmology & Visual Science, 2003

Research paper thumbnail of Two-photon Microscopy to Visualize Amyloid Plaques in Unstained Retinas from Alzheimer’s Disease

Investigative Ophthalmology & Visual Science, Jun 11, 2015

Research paper thumbnail of Impact of the Retinal Reflection on the Wide-Angle Point Spread Function of the Human Eye

Investigative Ophthalmology & Visual Science, Jun 16, 2013

Research paper thumbnail of Retinal Cell Density In Chicks After Deprivation Myopia Measured With Adaptive-Optics Multiphoton Microscopy

Investigative Ophthalmology & Visual Science, Mar 26, 2012

Research paper thumbnail of Purkinje Imaging System to Objectively Measure Corneal Haze and Lenticular Scattering

Investigative Ophthalmology & Visual Science, May 10, 2007

Research paper thumbnail of Subjective Evaluation of Polarisation Images of the Optic Nerve Head and Retinal Structures

Investigative Ophthalmology & Visual Science, 2006

Research paper thumbnail of Improvement Of Confocal Scanning Laser ophthalmoscope Images Using Polarimetry

Investigative Ophthalmology & Visual Science, 2002

Research paper thumbnail of Collagen Crosslinking By Infrared Femtosecond Pulses In Ex-vivo Corneas

Investigative Ophthalmology & Visual Science, 2013

Research paper thumbnail of Two-photon Microscopy to Visualize Amyloid Plaques in Unstained Retinas from Alzheimer’s Disease

Investigative Ophthalmology & Visual Science, 2015

Research paper thumbnail of Quantifying the Quality of Confocal Scanning Laser Ophthalmoscopic Images of Blood Vessels

Investigative Ophthalmology & Visual Science, 2007

Research paper thumbnail of Interferometic and Hartmann-Shack Measurements of Aberrations in ex-vivo Human Crystalline Lenses

Investigative Ophthalmology & Visual Science, 2009

Research paper thumbnail of Polarimetry of the Optic Nerve Head and Lamina Cribrosa

Investigative Ophthalmology & Visual Science, 2004

Research paper thumbnail of Modeling Aberrations From Surface Measurements of ex-vivo Isolated Human Crystalline Lenses

Investigative Ophthalmology & Visual Science, 2009

Research paper thumbnail of Towards a background-free neutrinoless double beta decay experiment based on a fluorescent bicolor sensor

arXiv: Instrumentation and Detectors, 2019

Neutrinoless double beta decay ($\beta\beta0\nu$) is a putative nuclear decay that can occur if, ... more Neutrinoless double beta decay ($\beta\beta0\nu$) is a putative nuclear decay that can occur if, and only if, neutrinos are their own antiparticles. Due to the smallness of neutrino masses, the lifetime of betabeta0nu\beta\beta0\nubetabeta0nu is expected to be larger than 102610^{26}1026 yr, and the tiny expected signals are deeply buried in backgrounds associated with the natural radioactive chains, whose characteristic lifetime is sixteen orders of magnitude faster. Future experiments aiming to explore lifetimes several orders of magnitude larger will require the development of new experimental techniques to suppress those backgrounds. Since no known background processes converts xenon to barium, detection of the daughter ion in candidate decay events effectively eliminates backgrounds. It has been recently proposed that a xenon gas time projection chamber could unambiguously tag the betabeta0nu\beta\beta0\nubetabeta0nu decay Xe136rightarrowBa2++2e−(+2nu)Xe^{136} \rightarrow Ba^{2+} + 2e^- (+2 \nu)Xe136rightarrowBa2++2e(+2nu) by detecting the resulting Ba2+Ba^{2+}Ba2+ ion in a single-a...

Research paper thumbnail of Metrics to Quantify the Global Quality of Confocal Scanning Laser Ophthalmoscopic Images of the Aging Eye

Investigative Ophthalmology & Visual Science, 2007

Research paper thumbnail of Novel Scanning Characterization Approaches for the Accurate Understanding and Successful Treatment of Oral and Maxillofacial Pathologies

Research paper thumbnail of Adaptive Optics Multiphoton Microscopy of ex-vivo Ocular Tissues

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