Juan Carlos Oropeza Basualdo - Academia.edu (original) (raw)
Papers by Juan Carlos Oropeza Basualdo
We evaluate the prevalence of intestinal parasites in 504 people and the degree of association be... more We evaluate the prevalence of intestinal parasites in 504 people and the degree of association between environmental variables and parasites found in population, soil and water in a rural area of Argentina during 2002-2003. A structured survey was used to evaluate the environmental variables and fecal-human, soil and water samples were analyzed. The prevalence of parasites was 45.4%. Most prevalent protozoa were Blastocystis hominis (27.2%) and Giardia lamblia (6.9%), while the most prevalent helminth was Ascaris lumbricoides (3.8%). The analyzed environmental variables showing association (p < 0.05) with presence of parasites in population were: cardboard-tin or wooden house, dirt floor, home or communal water pump, faucet outside the house or public faucet and cesspool or latrine. Parasite forms were found in 82.3% of the soil samples and in 84.2% of the water samples. In both samples we found parasites that were also found in people. In this study we have found deficient sanit...
We evaluate the prevalence of intestinal parasites in 504 people and the degree of association be... more We evaluate the prevalence of intestinal parasites in 504 people and the degree of association between environmental variables and parasites found in population, soil and water in a rural area of Argentina during 2002-2003. A structured survey was used to evaluate the environmental variables and fecal-human, soil and water samples were analyzed. The prevalence of parasites was 45.4%. Most prevalent protozoa were Blastocystis hominis (27.2%) and Giardia lamblia (6.9%), while the most prevalent helminth was Ascaris lumbricoides (3.8%). The analyzed environmental variables showing association (p < 0.05) with presence of parasites in population were: cardboard-tin or wooden house, dirt floor, home or communal water pump, faucet outside the house or public faucet and cesspool or latrine. Parasite forms were found in 82.3 % of the soil samples and in 84.2 % of the water samples. In both samples we found parasites that were also found in people. In this study we have found deficient san...
El objetivo del trabajo fue determinar la actividad inhibitoria de la fase liquida (FL) del fango... more El objetivo del trabajo fue determinar la actividad inhibitoria de la fase liquida (FL) del fango termal de Copahue, Neuquen, Argentina, sobre la microbiota de piel, fosas nasales, intestinal y vaginal. Las cepas utilizadas fueron Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis y Candida albicans. Las muestras de fango se obtuvieron de 5 sitios de la Laguna Sulfurosa. La actividad inhibitoria de la FL se demostro utilizando pruebas de difusion en agar y velocidad bactericida. Con la prueba de difusion en agar se observo halo inhibitorio para S. aureus y C. albicans y no se visualizo para S. epidermidis y E. faecalis El estudio dinamico de la actividad sobre S. aureus, E. faecalis y C. albicans demostro accion bactericida (disminucion > 3 log.) a las 6 horas de exposicion y recrecimiento con incubacion de 24 h. En S. epidermidis se observo accion bactericida a las 4 horas. La discordancia entre la prueba de difusion y la velocidad bactericida demostro la e...
El Complejo Termal de Copahue se situa a unos 1980 m sobre el nivel del mar, a 18 Km del volcan C... more El Complejo Termal de Copahue se situa a unos 1980 m sobre el nivel del mar, a 18 Km del volcan Copahue, sobre la cordillera de los Andes, Neuquen, Argentina. En el se halla la laguna Madre cuya temperatura oscila entre 70° y 90o C. En el fondo de la laguna se encuentra el fango gris, compuesto principalmente por azufre, silicio, oxigeno y aluminio. Empiricamente se conocen propiedades antiflogisticas y antibacterianas de los fangos minerales. Actualmente son utilizados en tratamientos de enfermedades de piel, reumaticas, infecciones de heridas quirurgicas y tambien con fines esteticos. Staphylococcus aureus es un microorganismo integrante de la microbiota humana y como colonizante de piel y mucosas esta implicado en diferentes procesos infecciosos. Su presencia en la microbiota del hombre aumenta el riesgo de padecer infecciones invasivas. La resistencia a antimicrobianos constituye un problema emergente en el tratamiento de este tipo de infecciones. El objetivo de este trabajo fue...
Pathogens, 2020
The study’s aim was to analyze the population structure of enterococci causing human invasive inf... more The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE/MLST/BAPS) were determined according to standard guidelines. β-lactamase production was determined by a nitrocefin test and confirmed by PCR/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9/ST179/ST236/ST281/ST388/ST604/ST720), were resistant to high-levels (HLR) of gentamicin/streptomycin. A ST9 clone (bla+/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25/ST18/ST19/ST52/ST792), with a low rate of ampicillin ...
Revista Argentina de microbiologia, Jan 5, 2017
The degree of antagonism exercised by fungi on geohelminth development varies according to the mo... more The degree of antagonism exercised by fungi on geohelminth development varies according to the morphological alterations caused by different fungal species. Saprophytic fungi may exert ovicidal or ovistatic effects. The aim of this study was to apply scanning electron microscopy (SEM) to observe the action of two soil saprophytic species of Chrysosporium (C. indicum and C. keratinophylum) on Toxocara canis eggs. The fungal strains to be tested were incubated for 28 days at 28°C in 2% water agar with a suspension of unembryonated T. canis eggs. A suspension of T. canis eggs in 2% water agar was used as control group. The assay was done in triplicate for each fungus and the control group. SEM observations were performed on the 4th, 7th, 14th, 21st, and 28th day after inoculation. The effect of the fungi on eggs was evaluated in accordance with the alterations observed on the surface and the changes in the normal characteristics of the eggs. Hyphae around the eggs, appresoria penetrati...
Revista Argentina de Microbiología, 2017
Resumen Blastocystis spp. es el protista intracelular que se detecta con mayor frecuencia en mues... more Resumen Blastocystis spp. es el protista intracelular que se detecta con mayor frecuencia en muestras de materia fecal humana; las tasas de infección pueden superar el 20% en países en vías de desarrollo. El hallazgo de este parásito en heces de diversas especies animales sugiere su potencial zoonótico. La relevancia clínica y el papel patógeno de Blastocystis spp. en el tracto intestinal son inciertos. Varias son las publicaciones que lo reconocen como agente etiológico de desórdenes intestinales como diarrea, enfermedad inflamatoria intestinal y colitis ulcerosa, aunque la patogenicidad de este parásito no ha sido probada. Este amplio rango de respuestas a la infección podría estar relacionado con la diversidad genética de los aislamientos provenientes de hospedadores infectados.
Veterinary Parasitology, 2016
First study about the development of adult Echinococcus canadensis G6 genotype of goat origin in ... more First study about the development of adult Echinococcus canadensis G6 genotype of goat origin in experimentally infected dogs.
Revista Argentina De Microbiologia, Sep 1, 2004
Rev Bras Educ Med, Dec 1, 1993
Journal of Medical Microbiology & Diagnosis, 2016
Tercera Epoca, Oct 1, 2010
Tercera Epoca, Dec 1, 2010
Parasitology Research, 2016
Cryptosporidium is an opportunistic protozoan parasite of humans and animals worldwide and causes... more Cryptosporidium is an opportunistic protozoan parasite of humans and animals worldwide and causes diarrheal disease that is typically self-limiting in immunocompetent hosts but often life threatening to immunocompromised individuals. However, there is a lack of completely efficient therapy available. Probiotics have attracted the attention as potential antiparasite compounds against protozoa involved in intestinal infections. This study investigated the effects of administration of probiotic Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in immunosuppressed mice. Effects on C. parvum infection at the intestinal mucosa were studied and scored at each portion of the gut. It was demonstrated that Ef CECT 7121 interfered with C. parvum infection when both probiotic and parasite were present in the same intestinal location suggesting that Ef CECT 7121 supplementation can alleviate the negative effects of C. parvum infection.
Enfoques Revista De La Universidad Adventista Del Plata, 2007
La articulación de la investigación con el grado y posgrado con medios existentes, cátedra de Met... more La articulación de la investigación con el grado y posgrado con medios existentes, cátedra de Metodología del Trabajo Científico y Servicio a la Comunidad de pregrado, y nuevos, con la integración a las cátedras de ayudantes alumnos de investigación, y becarios de entrenamiento. En posgrado se implementan Proyectos de Investigación y formación de recursos humanos con las figuras de becario de iniciación, perfeccionamiento y externo. Palabras clave: articulación de investigación-ayudantes de investigación-becarios-proyectos de investigación Summary We present a plan for the organization and development of research activities in the School of Health Sciences at Plate Adventist University. The plan for the articulation of research with graduate and postgraduate teaching has a schematic design: research activities are connected to undergraduate and graduate classes through the Methodology of Research course, the Undergraduate Community Service Program and the recent incorporation of Teacher Assistant positions and Undergraduate Fellowship in research. At a postgraduate level we carry out research projects and shape the human resource through the Fellowship in Research, which offers initiation, advanced and extern positions.
Acta Bioquim Clin Latinoam, Dec 1, 2001
Tercera Epoca, Oct 1, 2010
Revista De La Sociedad Venezolana De Microbiologia, Dec 1, 2013
Resumen: En Enterococcus spp. aislados de quesos de oveja se investigó la resistencia in vitro a ... more Resumen: En Enterococcus spp. aislados de quesos de oveja se investigó la resistencia in vitro a antimicrobianos de utilización clínica. Se determinó la concentración inhibitoria mínima (CIM) para gentamicina, estreptomicina, vancomicina, teicoplanina, ampicilina, imipenem, linezolid y tigeciclina. Se detectó la producción de β-lactamasa y se realizaron experimentos de conjugación para transferir resistencia a gentamicina. En un aislamiento de E. faecalis y en 10 de E. faecium se observó resistencia a ampicilina; se demostró la producción de β-lactamasa en un E. faecalis. En E. faecium, 15 aislamientos fueron resistentes a imipenem; 4 a linezolid y 5 a glucopéptidos. En 4 aislamientos de E. faecalis se demostró alto nivel de resistencia plasmídica a gentamicina. No se observaron aislamientos resistentes a tigeciclina. El queso de oveja es un reservorio de enterococos resistentes con potencial diseminación al hombre a través de la cadena alimentaria.
Ciencia Docencia Y Tecnologia, Dec 1, 2011
We evaluate the prevalence of intestinal parasites in 504 people and the degree of association be... more We evaluate the prevalence of intestinal parasites in 504 people and the degree of association between environmental variables and parasites found in population, soil and water in a rural area of Argentina during 2002-2003. A structured survey was used to evaluate the environmental variables and fecal-human, soil and water samples were analyzed. The prevalence of parasites was 45.4%. Most prevalent protozoa were Blastocystis hominis (27.2%) and Giardia lamblia (6.9%), while the most prevalent helminth was Ascaris lumbricoides (3.8%). The analyzed environmental variables showing association (p < 0.05) with presence of parasites in population were: cardboard-tin or wooden house, dirt floor, home or communal water pump, faucet outside the house or public faucet and cesspool or latrine. Parasite forms were found in 82.3% of the soil samples and in 84.2% of the water samples. In both samples we found parasites that were also found in people. In this study we have found deficient sanit...
We evaluate the prevalence of intestinal parasites in 504 people and the degree of association be... more We evaluate the prevalence of intestinal parasites in 504 people and the degree of association between environmental variables and parasites found in population, soil and water in a rural area of Argentina during 2002-2003. A structured survey was used to evaluate the environmental variables and fecal-human, soil and water samples were analyzed. The prevalence of parasites was 45.4%. Most prevalent protozoa were Blastocystis hominis (27.2%) and Giardia lamblia (6.9%), while the most prevalent helminth was Ascaris lumbricoides (3.8%). The analyzed environmental variables showing association (p < 0.05) with presence of parasites in population were: cardboard-tin or wooden house, dirt floor, home or communal water pump, faucet outside the house or public faucet and cesspool or latrine. Parasite forms were found in 82.3 % of the soil samples and in 84.2 % of the water samples. In both samples we found parasites that were also found in people. In this study we have found deficient san...
El objetivo del trabajo fue determinar la actividad inhibitoria de la fase liquida (FL) del fango... more El objetivo del trabajo fue determinar la actividad inhibitoria de la fase liquida (FL) del fango termal de Copahue, Neuquen, Argentina, sobre la microbiota de piel, fosas nasales, intestinal y vaginal. Las cepas utilizadas fueron Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis y Candida albicans. Las muestras de fango se obtuvieron de 5 sitios de la Laguna Sulfurosa. La actividad inhibitoria de la FL se demostro utilizando pruebas de difusion en agar y velocidad bactericida. Con la prueba de difusion en agar se observo halo inhibitorio para S. aureus y C. albicans y no se visualizo para S. epidermidis y E. faecalis El estudio dinamico de la actividad sobre S. aureus, E. faecalis y C. albicans demostro accion bactericida (disminucion > 3 log.) a las 6 horas de exposicion y recrecimiento con incubacion de 24 h. En S. epidermidis se observo accion bactericida a las 4 horas. La discordancia entre la prueba de difusion y la velocidad bactericida demostro la e...
El Complejo Termal de Copahue se situa a unos 1980 m sobre el nivel del mar, a 18 Km del volcan C... more El Complejo Termal de Copahue se situa a unos 1980 m sobre el nivel del mar, a 18 Km del volcan Copahue, sobre la cordillera de los Andes, Neuquen, Argentina. En el se halla la laguna Madre cuya temperatura oscila entre 70° y 90o C. En el fondo de la laguna se encuentra el fango gris, compuesto principalmente por azufre, silicio, oxigeno y aluminio. Empiricamente se conocen propiedades antiflogisticas y antibacterianas de los fangos minerales. Actualmente son utilizados en tratamientos de enfermedades de piel, reumaticas, infecciones de heridas quirurgicas y tambien con fines esteticos. Staphylococcus aureus es un microorganismo integrante de la microbiota humana y como colonizante de piel y mucosas esta implicado en diferentes procesos infecciosos. Su presencia en la microbiota del hombre aumenta el riesgo de padecer infecciones invasivas. La resistencia a antimicrobianos constituye un problema emergente en el tratamiento de este tipo de infecciones. El objetivo de este trabajo fue...
Pathogens, 2020
The study’s aim was to analyze the population structure of enterococci causing human invasive inf... more The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE/MLST/BAPS) were determined according to standard guidelines. β-lactamase production was determined by a nitrocefin test and confirmed by PCR/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9/ST179/ST236/ST281/ST388/ST604/ST720), were resistant to high-levels (HLR) of gentamicin/streptomycin. A ST9 clone (bla+/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25/ST18/ST19/ST52/ST792), with a low rate of ampicillin ...
Revista Argentina de microbiologia, Jan 5, 2017
The degree of antagonism exercised by fungi on geohelminth development varies according to the mo... more The degree of antagonism exercised by fungi on geohelminth development varies according to the morphological alterations caused by different fungal species. Saprophytic fungi may exert ovicidal or ovistatic effects. The aim of this study was to apply scanning electron microscopy (SEM) to observe the action of two soil saprophytic species of Chrysosporium (C. indicum and C. keratinophylum) on Toxocara canis eggs. The fungal strains to be tested were incubated for 28 days at 28°C in 2% water agar with a suspension of unembryonated T. canis eggs. A suspension of T. canis eggs in 2% water agar was used as control group. The assay was done in triplicate for each fungus and the control group. SEM observations were performed on the 4th, 7th, 14th, 21st, and 28th day after inoculation. The effect of the fungi on eggs was evaluated in accordance with the alterations observed on the surface and the changes in the normal characteristics of the eggs. Hyphae around the eggs, appresoria penetrati...
Revista Argentina de Microbiología, 2017
Resumen Blastocystis spp. es el protista intracelular que se detecta con mayor frecuencia en mues... more Resumen Blastocystis spp. es el protista intracelular que se detecta con mayor frecuencia en muestras de materia fecal humana; las tasas de infección pueden superar el 20% en países en vías de desarrollo. El hallazgo de este parásito en heces de diversas especies animales sugiere su potencial zoonótico. La relevancia clínica y el papel patógeno de Blastocystis spp. en el tracto intestinal son inciertos. Varias son las publicaciones que lo reconocen como agente etiológico de desórdenes intestinales como diarrea, enfermedad inflamatoria intestinal y colitis ulcerosa, aunque la patogenicidad de este parásito no ha sido probada. Este amplio rango de respuestas a la infección podría estar relacionado con la diversidad genética de los aislamientos provenientes de hospedadores infectados.
Veterinary Parasitology, 2016
First study about the development of adult Echinococcus canadensis G6 genotype of goat origin in ... more First study about the development of adult Echinococcus canadensis G6 genotype of goat origin in experimentally infected dogs.
Revista Argentina De Microbiologia, Sep 1, 2004
Rev Bras Educ Med, Dec 1, 1993
Journal of Medical Microbiology & Diagnosis, 2016
Tercera Epoca, Oct 1, 2010
Tercera Epoca, Dec 1, 2010
Parasitology Research, 2016
Cryptosporidium is an opportunistic protozoan parasite of humans and animals worldwide and causes... more Cryptosporidium is an opportunistic protozoan parasite of humans and animals worldwide and causes diarrheal disease that is typically self-limiting in immunocompetent hosts but often life threatening to immunocompromised individuals. However, there is a lack of completely efficient therapy available. Probiotics have attracted the attention as potential antiparasite compounds against protozoa involved in intestinal infections. This study investigated the effects of administration of probiotic Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in immunosuppressed mice. Effects on C. parvum infection at the intestinal mucosa were studied and scored at each portion of the gut. It was demonstrated that Ef CECT 7121 interfered with C. parvum infection when both probiotic and parasite were present in the same intestinal location suggesting that Ef CECT 7121 supplementation can alleviate the negative effects of C. parvum infection.
Enfoques Revista De La Universidad Adventista Del Plata, 2007
La articulación de la investigación con el grado y posgrado con medios existentes, cátedra de Met... more La articulación de la investigación con el grado y posgrado con medios existentes, cátedra de Metodología del Trabajo Científico y Servicio a la Comunidad de pregrado, y nuevos, con la integración a las cátedras de ayudantes alumnos de investigación, y becarios de entrenamiento. En posgrado se implementan Proyectos de Investigación y formación de recursos humanos con las figuras de becario de iniciación, perfeccionamiento y externo. Palabras clave: articulación de investigación-ayudantes de investigación-becarios-proyectos de investigación Summary We present a plan for the organization and development of research activities in the School of Health Sciences at Plate Adventist University. The plan for the articulation of research with graduate and postgraduate teaching has a schematic design: research activities are connected to undergraduate and graduate classes through the Methodology of Research course, the Undergraduate Community Service Program and the recent incorporation of Teacher Assistant positions and Undergraduate Fellowship in research. At a postgraduate level we carry out research projects and shape the human resource through the Fellowship in Research, which offers initiation, advanced and extern positions.
Acta Bioquim Clin Latinoam, Dec 1, 2001
Tercera Epoca, Oct 1, 2010
Revista De La Sociedad Venezolana De Microbiologia, Dec 1, 2013
Resumen: En Enterococcus spp. aislados de quesos de oveja se investigó la resistencia in vitro a ... more Resumen: En Enterococcus spp. aislados de quesos de oveja se investigó la resistencia in vitro a antimicrobianos de utilización clínica. Se determinó la concentración inhibitoria mínima (CIM) para gentamicina, estreptomicina, vancomicina, teicoplanina, ampicilina, imipenem, linezolid y tigeciclina. Se detectó la producción de β-lactamasa y se realizaron experimentos de conjugación para transferir resistencia a gentamicina. En un aislamiento de E. faecalis y en 10 de E. faecium se observó resistencia a ampicilina; se demostró la producción de β-lactamasa en un E. faecalis. En E. faecium, 15 aislamientos fueron resistentes a imipenem; 4 a linezolid y 5 a glucopéptidos. En 4 aislamientos de E. faecalis se demostró alto nivel de resistencia plasmídica a gentamicina. No se observaron aislamientos resistentes a tigeciclina. El queso de oveja es un reservorio de enterococos resistentes con potencial diseminación al hombre a través de la cadena alimentaria.
Ciencia Docencia Y Tecnologia, Dec 1, 2011