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Papers by Juliana Zanetti Ribeiro
The 100% Recyclable School project started in 2014 with the objective of sensitizing and making s... more The 100% Recyclable School project started in 2014 with the objective of sensitizing and making students and employees aware of the importance of the environment, centered on the 5R’s methodology: Refuse, Rethink, Reduce, Reuse and Recycle. It occurs through adherence, with diagnosis, lecture, play activities and monitoring. It was implemented in 23 school units, totaling 281 classes, 7316 students and 979 employees. From the results it is possible to point out that it is through compromised pedagogical attitudes can be changed and new habits can be built. It is precisely the dissemination, reflection and construction of knowledge about correct environmental practices that drive Environmental Education.
Conservation Genetics, 2015
In order to understand the impacts of forest fragmentation on Araucaria angustifolia populations,... more In order to understand the impacts of forest fragmentation on Araucaria angustifolia populations, we evaluated the genetic diversity and mating system using SSR markers and open-pollinated seeds from four populations of varying sizes and spatial isolation, in and around one of the best-conserved Araucaria Forest remnants in Southern Brazil. The four population types of A. angustifolia include: (1) a continuous forest; (2) a physically isolated cluster located 2 km from the continuous forest; (3) an open population in a field located between the cluster and continuous forest; and (4) a fragment on a private property located 5 km from the cluster. Approximately 28 seeds were collected from ten reproductive trees in each population. We found higher amounts of alleles (113) and exclusive alleles (25) in the continuous forest than in the other populations. The multilocus paternity correlation was significantly higher and effective number of pollen donors was significantly lower in the private population, decreasing the diversity and consequently the variance effective size of families sampled from that population. However, despite its isolation from the other studied fragments, the private population had the second highest number of alleles as well as unique alleles from the other populations. Therefore, strategies for A. angustifolia conservation should focus not only on larger populations, such as those found in protected areas, but also include smaller and isolated fragments on private properties as these populations are able to maintain high levels of genetic diversity and functional connectivity between isolated stands across a landscape.
Tree Genetics & Genomes, 2014
In this study, genetic diversity, inbreeding, spatial genetic structure (SGS), and pollen dispers... more In this study, genetic diversity, inbreeding, spatial genetic structure (SGS), and pollen dispersal are analyzed using ten microsatellite loci from two populations of the dioecious, wind-pollinated, coniferous tree Araucaria angustifolia in Southern Brazil. The study populations include an undisturbed 7 ha A. angustifolia cluster, where all adult trees were mapped and sampled, and an adjacent, long-abandoned, open agricultural area with an aggregated A. angustifolia population. Seeds were collected from 13 seed trees inside the forest cluster and from eight seed trees in the open forest. Our results showed that the adults present high levels of heterozygosity (H o =0.91) and an absence of inbreeding. However, significant SGS was detected up to 90 m in the forest cluster suggesting that near-neighbor trees are related. The estimate of effective population size was lower than the total number of trees in the cluster (N e /N=0.19), which can be explained by the presence of SGS in the stand. Substantial external pollen flow was detected in the forest cluster (26 %) and open forest (20 %), indicating that the reproductive population size is greater than the sampled populations, explaining the high genetic diversity in this population. Our results indicate that this site has potential for in and ex situ conservation due to high levels of genetic diversity and gene immigration resulting from pollen flow. Conservation strategies for A. angustifolia should focus not only on forest fragments but should also include the preservation of isolated trees throughout the landscape.
BMC Proceedings, 2014
Background Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces... more Background Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces through outcrossing. However, some monoecious trees have been identified and they may reproduce through self-fertilization. The objective of this study was to confirm the expected relatedness of self-sibs of hand self-pollinated monoecious seed trees, and to investigate the mating system of open-pollinated progenies of monoecious A. angustifolia trees.
Plant Systematics and Evolution, 2013
ABSTRACT Understanding the mating system of a tree species is important in genetic conservation a... more ABSTRACT Understanding the mating system of a tree species is important in genetic conservation and tree breeding strategies because it affects the inbreeding and genetic diversity of the descendant populations. Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces through outcrossing. However, some monoecious trees have been identified and they may reproduce through self-fertilization. The objective of this study was to confirm the expected relatedness of full-sibs in outcrossed hand-pollinated progenies of female seed trees, self-sibs of hand self-pollinated monoecious seed trees, and to investigate the mating system of open-pollinated progenies of female and monoecious A. angustifolia trees. To do this, eight microsatellite loci were used to genotype hand- and open-pollinated progenies. Our results show that the relatedness of outcrossed hand-pollinated progenies are true full-sibs and progenies from a selfed monoecious seed tree are self-sibs, which confirms the hand-pollination method used. Open-pollinated female seed trees reproduced only by outcrossing, generating progenies with a mixture of full- and half-sibs. Monoecious seed trees reproduced mainly by xenogamy, generating progenies with mixtures of self-sibs, full-sibs, half-sibs and self-half-sibs. We also found that an increase in the effective number of pollen donors ( $ N_{\text{ep}} $ ) would lead to an increase in the total number of alleles ( $ K $ ) within progenies. Our results also suggest that monecious trees have limited potential to modify the genetic structure through selfed seed production due to the very low estimated selfing rate in these trees and the rare occurrence of these trees in natural populations.
Silvae Genetica, 2013
In order to use molecular markers in population genetics studies, it is important to confirm that... more In order to use molecular markers in population genetics studies, it is important to confirm that the molecular markers used present a Mendelian segregation. The aim of this paper was to investigate the genetic segregation of eight microsatellite loci of Araucaria angustifolia (Bert.) O. Kuntze (Araucariaceae). The study was carried out comparing genetic segregation in hand- and open-pollinated progenies of maternal dioecious and monoecious trees. The Mendelian segregation was confirmed for all eight loci studied (Ag20, Ag23, Ag45, Aang01, Aang14, Aang28, As90 and CRCAc1), as no deviation from the expected segregation hypothesis was detected in the studied progenies. Therefore, these eight loci can be used for further population genetics studies of A. angustifolia.
Pesquisa Florestal Brasileira, 2012
O objetivo deste trabalho foi realizar a estratificação de ambientes de cultivo de milho nos Esta... more O objetivo deste trabalho foi realizar a estratificação de ambientes de cultivo de milho nos Estados do Paraná, Minas Gerais e Bahia, por diferentes métodos, bem como determinar o grau de associação e divergência entre os métodos de estratificação. Foram avaliados quatro métodos: o tradicional de Lin; o de dissimilaridade ambiental; o de decomposição da interação genótipo x ambiente (GxA) em partes simples e complexa; e o de análise de fatores. Esses métodos foram aplicados a 48 híbridos experimentais de milho, avaliados em 11 ambientes de cultivo nos três Estados, divididos em dois conjuntos de experimentos. Além dos híbridos, foram avaliadas seis cultivares comerciais, utilizadas como testemunhas, comuns aos dois conjuntos. Verificou-se a predominância de interação GxA do tipo complexa. A decomposição da interação GxA em partes simples e complexa e a análise de fatores são métodos fortemente associados entre si, mas moderadamente associados aos demais. Além disso, esses métodos são mais rigorosos no processo de estratificação ambiental e ponderam de maneira mais eficiente a magnitude da interação GxA.
Plant Systematics and Evolution, 2013
ABSTRACT Understanding the mating system of a tree species is important in genetic conservation a... more ABSTRACT Understanding the mating system of a tree species is important in genetic conservation and tree breeding strategies because it affects the inbreeding and genetic diversity of the descendant populations. Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces through outcrossing. However, some monoecious trees have been identified and they may reproduce through self-fertilization. The objective of this study was to confirm the expected relatedness of full-sibs in outcrossed hand-pollinated progenies of female seed trees, self-sibs of hand self-pollinated monoecious seed trees, and to investigate the mating system of open-pollinated progenies of female and monoecious A. angustifolia trees. To do this, eight microsatellite loci were used to genotype hand- and open-pollinated progenies. Our results show that the relatedness of outcrossed hand-pollinated progenies are true full-sibs and progenies from a selfed monoecious seed tree are self-sibs, which confirms the hand-pollination method used. Open-pollinated female seed trees reproduced only by outcrossing, generating progenies with a mixture of full- and half-sibs. Monoecious seed trees reproduced mainly by xenogamy, generating progenies with mixtures of self-sibs, full-sibs, half-sibs and self-half-sibs. We also found that an increase in the effective number of pollen donors ( $ N_{\text{ep}} $ ) would lead to an increase in the total number of alleles ( $ K $ ) within progenies. Our results also suggest that monecious trees have limited potential to modify the genetic structure through selfed seed production due to the very low estimated selfing rate in these trees and the rare occurrence of these trees in natural populations.
The 100% Recyclable School project started in 2014 with the objective of sensitizing and making s... more The 100% Recyclable School project started in 2014 with the objective of sensitizing and making students and employees aware of the importance of the environment, centered on the 5R’s methodology: Refuse, Rethink, Reduce, Reuse and Recycle. It occurs through adherence, with diagnosis, lecture, play activities and monitoring. It was implemented in 23 school units, totaling 281 classes, 7316 students and 979 employees. From the results it is possible to point out that it is through compromised pedagogical attitudes can be changed and new habits can be built. It is precisely the dissemination, reflection and construction of knowledge about correct environmental practices that drive Environmental Education.
Conservation Genetics, 2015
In order to understand the impacts of forest fragmentation on Araucaria angustifolia populations,... more In order to understand the impacts of forest fragmentation on Araucaria angustifolia populations, we evaluated the genetic diversity and mating system using SSR markers and open-pollinated seeds from four populations of varying sizes and spatial isolation, in and around one of the best-conserved Araucaria Forest remnants in Southern Brazil. The four population types of A. angustifolia include: (1) a continuous forest; (2) a physically isolated cluster located 2 km from the continuous forest; (3) an open population in a field located between the cluster and continuous forest; and (4) a fragment on a private property located 5 km from the cluster. Approximately 28 seeds were collected from ten reproductive trees in each population. We found higher amounts of alleles (113) and exclusive alleles (25) in the continuous forest than in the other populations. The multilocus paternity correlation was significantly higher and effective number of pollen donors was significantly lower in the private population, decreasing the diversity and consequently the variance effective size of families sampled from that population. However, despite its isolation from the other studied fragments, the private population had the second highest number of alleles as well as unique alleles from the other populations. Therefore, strategies for A. angustifolia conservation should focus not only on larger populations, such as those found in protected areas, but also include smaller and isolated fragments on private properties as these populations are able to maintain high levels of genetic diversity and functional connectivity between isolated stands across a landscape.
Tree Genetics & Genomes, 2014
In this study, genetic diversity, inbreeding, spatial genetic structure (SGS), and pollen dispers... more In this study, genetic diversity, inbreeding, spatial genetic structure (SGS), and pollen dispersal are analyzed using ten microsatellite loci from two populations of the dioecious, wind-pollinated, coniferous tree Araucaria angustifolia in Southern Brazil. The study populations include an undisturbed 7 ha A. angustifolia cluster, where all adult trees were mapped and sampled, and an adjacent, long-abandoned, open agricultural area with an aggregated A. angustifolia population. Seeds were collected from 13 seed trees inside the forest cluster and from eight seed trees in the open forest. Our results showed that the adults present high levels of heterozygosity (H o =0.91) and an absence of inbreeding. However, significant SGS was detected up to 90 m in the forest cluster suggesting that near-neighbor trees are related. The estimate of effective population size was lower than the total number of trees in the cluster (N e /N=0.19), which can be explained by the presence of SGS in the stand. Substantial external pollen flow was detected in the forest cluster (26 %) and open forest (20 %), indicating that the reproductive population size is greater than the sampled populations, explaining the high genetic diversity in this population. Our results indicate that this site has potential for in and ex situ conservation due to high levels of genetic diversity and gene immigration resulting from pollen flow. Conservation strategies for A. angustifolia should focus not only on forest fragments but should also include the preservation of isolated trees throughout the landscape.
BMC Proceedings, 2014
Background Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces... more Background Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces through outcrossing. However, some monoecious trees have been identified and they may reproduce through self-fertilization. The objective of this study was to confirm the expected relatedness of self-sibs of hand self-pollinated monoecious seed trees, and to investigate the mating system of open-pollinated progenies of monoecious A. angustifolia trees.
Plant Systematics and Evolution, 2013
ABSTRACT Understanding the mating system of a tree species is important in genetic conservation a... more ABSTRACT Understanding the mating system of a tree species is important in genetic conservation and tree breeding strategies because it affects the inbreeding and genetic diversity of the descendant populations. Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces through outcrossing. However, some monoecious trees have been identified and they may reproduce through self-fertilization. The objective of this study was to confirm the expected relatedness of full-sibs in outcrossed hand-pollinated progenies of female seed trees, self-sibs of hand self-pollinated monoecious seed trees, and to investigate the mating system of open-pollinated progenies of female and monoecious A. angustifolia trees. To do this, eight microsatellite loci were used to genotype hand- and open-pollinated progenies. Our results show that the relatedness of outcrossed hand-pollinated progenies are true full-sibs and progenies from a selfed monoecious seed tree are self-sibs, which confirms the hand-pollination method used. Open-pollinated female seed trees reproduced only by outcrossing, generating progenies with a mixture of full- and half-sibs. Monoecious seed trees reproduced mainly by xenogamy, generating progenies with mixtures of self-sibs, full-sibs, half-sibs and self-half-sibs. We also found that an increase in the effective number of pollen donors ( $ N_{\text{ep}} $ ) would lead to an increase in the total number of alleles ( $ K $ ) within progenies. Our results also suggest that monecious trees have limited potential to modify the genetic structure through selfed seed production due to the very low estimated selfing rate in these trees and the rare occurrence of these trees in natural populations.
Silvae Genetica, 2013
In order to use molecular markers in population genetics studies, it is important to confirm that... more In order to use molecular markers in population genetics studies, it is important to confirm that the molecular markers used present a Mendelian segregation. The aim of this paper was to investigate the genetic segregation of eight microsatellite loci of Araucaria angustifolia (Bert.) O. Kuntze (Araucariaceae). The study was carried out comparing genetic segregation in hand- and open-pollinated progenies of maternal dioecious and monoecious trees. The Mendelian segregation was confirmed for all eight loci studied (Ag20, Ag23, Ag45, Aang01, Aang14, Aang28, As90 and CRCAc1), as no deviation from the expected segregation hypothesis was detected in the studied progenies. Therefore, these eight loci can be used for further population genetics studies of A. angustifolia.
Pesquisa Florestal Brasileira, 2012
O objetivo deste trabalho foi realizar a estratificação de ambientes de cultivo de milho nos Esta... more O objetivo deste trabalho foi realizar a estratificação de ambientes de cultivo de milho nos Estados do Paraná, Minas Gerais e Bahia, por diferentes métodos, bem como determinar o grau de associação e divergência entre os métodos de estratificação. Foram avaliados quatro métodos: o tradicional de Lin; o de dissimilaridade ambiental; o de decomposição da interação genótipo x ambiente (GxA) em partes simples e complexa; e o de análise de fatores. Esses métodos foram aplicados a 48 híbridos experimentais de milho, avaliados em 11 ambientes de cultivo nos três Estados, divididos em dois conjuntos de experimentos. Além dos híbridos, foram avaliadas seis cultivares comerciais, utilizadas como testemunhas, comuns aos dois conjuntos. Verificou-se a predominância de interação GxA do tipo complexa. A decomposição da interação GxA em partes simples e complexa e a análise de fatores são métodos fortemente associados entre si, mas moderadamente associados aos demais. Além disso, esses métodos são mais rigorosos no processo de estratificação ambiental e ponderam de maneira mais eficiente a magnitude da interação GxA.
Plant Systematics and Evolution, 2013
ABSTRACT Understanding the mating system of a tree species is important in genetic conservation a... more ABSTRACT Understanding the mating system of a tree species is important in genetic conservation and tree breeding strategies because it affects the inbreeding and genetic diversity of the descendant populations. Araucaria angustifolia (Bert.) O. Kuntze is a mainly dioecious species that reproduces through outcrossing. However, some monoecious trees have been identified and they may reproduce through self-fertilization. The objective of this study was to confirm the expected relatedness of full-sibs in outcrossed hand-pollinated progenies of female seed trees, self-sibs of hand self-pollinated monoecious seed trees, and to investigate the mating system of open-pollinated progenies of female and monoecious A. angustifolia trees. To do this, eight microsatellite loci were used to genotype hand- and open-pollinated progenies. Our results show that the relatedness of outcrossed hand-pollinated progenies are true full-sibs and progenies from a selfed monoecious seed tree are self-sibs, which confirms the hand-pollination method used. Open-pollinated female seed trees reproduced only by outcrossing, generating progenies with a mixture of full- and half-sibs. Monoecious seed trees reproduced mainly by xenogamy, generating progenies with mixtures of self-sibs, full-sibs, half-sibs and self-half-sibs. We also found that an increase in the effective number of pollen donors ( $ N_{\text{ep}} $ ) would lead to an increase in the total number of alleles ( $ K $ ) within progenies. Our results also suggest that monecious trees have limited potential to modify the genetic structure through selfed seed production due to the very low estimated selfing rate in these trees and the rare occurrence of these trees in natural populations.