Younghun Jung - Academia.edu (original) (raw)

Papers by Younghun Jung

Growth Arrest-Specific 6 (GAS6) Promotes Prostate Cancer Survival by G1 Arrest/S Phase Delay and Inhibition of Apoptotic Pathway During Chemotherapy in Bone Marrow

Journal of cellular biochemistry, Jan 5, 2016

Prostate cancer (PCa) is known to develop resistance to chemotherapy. Growth arrest-specific 6 (G... more Prostate cancer (PCa) is known to develop resistance to chemotherapy. Growth arrest-specific 6 (GAS6), plays a role in tumor progression by regulating growth in many cancers. Here, we explored how GAS6 regulates the cell cycle and apoptosis of PCa cells in response to chemotherapy. We found that GAS6 is sufficient to significantly increase the number and duration of G1 phase in PCa cells. Importantly, GAS6 further increased the number of G1 arrested cells during docetaxel chemotherapy. GAS6 altered the signals of key cell cycle regulators: Cyclin B1 (G2 /M phase), CDC25A, Cyclin E1, and CDK2 (S phase entry) were all downregulated, while p27, p21, Cyclin D1, and CDK4 (G0 /G1 phase) were upregulated. Importantly, these signaling events were further accentuated during docetaxel treatment in the presence of GAS6. Moreover, the apoptotic response of PCa cells to GAS6 was examined during docetaxel chemotherapy. Docetaxel induced PCa cell apoptosis. However, this apoptotic response was abr...

Endogenous GAS6 and Mer receptor signaling regulate prostate cancer stem cells in bone marrow

Oncotarget, Jan 25, 2016

GAS6 and its receptors (Tryo 3, Axl, Mer or "TAM") are known to play a role in regulati... more GAS6 and its receptors (Tryo 3, Axl, Mer or "TAM") are known to play a role in regulating tumor progression in a number of settings. Previously we have demonstrated that GAS6 signaling regulates invasion, proliferation, chemotherapy-induced apoptosis of prostate cancer (PCa) cells. We have also demonstrated that GAS6 secreted from osteoblasts in the bone marrow environment plays a critical role in establishing prostate tumor cell dormancy. Here we investigated the role that endogenous GAS6 and Mer receptor signaling plays in establishing prostate cancer stem cells in the bone marrow microenvironment.We first observed that high levels of endogenous GAS6 are expressed by disseminated tumor cells (DTCs) in the bone marrow, whereas relatively low levels of endogenous GAS6 are expressed in PCa tumors grown in a s.c. Interestingly, elevated levels of endogenous GAS6 were identified in putative cancer stem cells (CSCs, CD133+/CD44+) compared to non-CSCs (CD133-/CD44-) isolated fr...

국내 경작지 특성을 고려한 SWAT 모형의 식생여과대 유사저감 효율 산정식 개선

Journal of Korean Society on Water Environment, 2015

A Survey of Disease Occurrence in Korean Black Goats

한국임상수의학회지, Apr 1, 2012

Effects of barley and barley bran contaminated with Fusarium spp. on the growth and feed efficiency of fattening and growing pigs

Post-annealing behavior of Ni/Au Schottky contact on non-polar a-plane GaN

Thin Solid Films, Aug 2, 2010

The effects of annealing on the performance of Schottky devices on a-plane GaN/r-plane sapphire w... more The effects of annealing on the performance of Schottky devices on a-plane GaN/r-plane sapphire were investigated. The results show that the post-anneal Schottky barrier height (SBH) increased with increasing annealing temperature, reaching a peak increase of 43% ...

Non-specific activation of mouse peritoneal macrophages by a freshwater ciliate, Tetrahymena pyriformis

The Korean Journal of Parasitology, Jun 30, 2000

Toxoplasma-killing activities of mouse peritoneal macrophages activated by the extracts of Tetrah... more Toxoplasma-killing activities of mouse peritoneal macrophages activated by the extracts of Tetrahymena pyriformis (Korean and Chinese strains) were evaluated, and the active protein fractions from both strains were partially characterized by a method including chromatographies and ...

A Serial Observation of Ovarian Fibroma in a Living Dairy Cow

한국임상수의학회지, Apr 1, 2011

Neoplasia, Sep 1, 2013

The mechanisms that regulate hematopoietic stem cell (HSC) dormancy and self-renewal are well est... more The mechanisms that regulate hematopoietic stem cell (HSC) dormancy and self-renewal are well established and are largely dependent on signals emanating from the HSC niche. Recently, we found that prostate cancer (PCa) cells target the HSC niche in mouse bone marrow (BM) during metastasis. Little is known, however, as to how the HSC niche may regulate dormancy in cancer cells. In this study, we investigated the effects of TANK binding kinase 1 (TBK1) on PCa dormancy in the BM niche. We found that binding with niche osteoblasts induces the expression of TBK1 in PCa cells PC3 and C4-2B. Interestingly, TBK1 interacts with mammalian target of rapamycin (mTOR) and inhibits its function. Rapamycin, an mTOR inhibitor, induces cell cycle arrest of PCa cells and enhances chemotherapeutic resistance of PCa cells. As a result, the knockdown of TBK1 decreases PCa stem-like cells and drug resistance in vitro and in vivo. Taken together, these results strongly indicate that TBK1 plays an important role in the dormancy and drug resistance of PCa.

SWAT 모형과 BFlow를 이용한 지하수 함양, 기저유출의 시공간적 변화 분석

Journal of Korean Society on Water Environment, 2014

도시화는 불투수면적의 증가로 인해 수문학적 변화를 초래하며 또한 지하수량의 감소와 지표유출의 증가와 같은 현상이 발생한다. 따라서 본 연구의 목적은 일유량을 기본으로 한 토지... more 도시화는 불투수면적의 증가로 인해 수문학적 변화를 초래하며 또한 지하수량의 감소와 지표유출의 증가와 같은 현상이 발생한다. 따라서 본 연구의 목적은 일유량을 기본으로 한 토지이용 변화 현상에 대한 영향을 정량적이고 공간적으로 다른 토지이용도를 이용하 여 산정하는 것이다. 이에 따라 본 연구에서는 ARCSWAT의 SWAT2005 버전을 이용하여 갑천 유역을 일유량으로 모의 하였다. 모형 의 검보정 후, 1997년부터 2001년 일유량으로 모의된 결과를 통계학적으로 분석하였고, 모의된 유량은 Mann-Whiteny method로 테스 트 하였다. 도시 면적이 28 % 차이가 있는 경우 SWAT의 유량 모의 결과는 5 % 유의수준에서 도시화의 영향이 모의된 유량에 크게 영향을 주는 것으로 나타났다. 그러나 도시화의 공간적 변화는 5 % 유의수준에서 유량의 변화에 미치는 영향이 거의 없는 것으로 나 타났다.

Evaluation of Regression Models in LOADEST to Estimate Suspended Solid Load in Hangang Waterbody

Advances in Meteorology, 2014

We performed bias correction in future climate change scenarios to provide better accuracy of mod... more We performed bias correction in future climate change scenarios to provide better accuracy of models through adaptation to future climate change. The proposed combination of the change factor (CF) and quantile mapping (QM) methods combines the individual advantages of both methods for adjusting the bias in global circulation models (GCMs) and regional circulation models (RCMs). We selected a study site in Songwol-dong, Seoul, Republic of Korea, to test and assess our proposed method. Our results show that the combined CF + QM method delivers better performance in terms of correcting the bias in GCMs/RCMs than when both methods are applied individually. In particular, our proposed method considerably improved the bias-corrected precipitation by capturing both the high peaks and amounts of precipitation as compared to that from the CF-only and QM-only methods. Thus, our proposed method can provide high-accuracy bias-corrected precipitation data, which could prove to be highly useful in interdisciplinary studies across the world.

Assessing the effect of watershed slopes on recharge/baseflow and soil erosion

Paddy and Water Environment, 2014

ABSTRACT Aquatic ecosystems are threatened by increasing variability in the hydrologic responses.... more ABSTRACT Aquatic ecosystems are threatened by increasing variability in the hydrologic responses. In particular, the health of river ecosystems in steeply sloping watersheds is aggravated due to soil erosion and stream depletion during dry periods. This study suggested and assessed a method to improve the adaptation ability of a river system in a steep watershed. For this, this study calibrated soil and water assessment tool (SWAT) for runoff and sediment, and quantified the changes in hydrologic responses such as groundwater recharge rate soil erosion and baseflow according to two scenarios for adjustment of the watershed slope (steep to mild). Here, one scenario was set by three measured slopes, and the other was set by fixing the entire watershed slopes with 5 %. Moreover, SWAT and web-based hydrograph analysis tool (WHAT) models were applied to estimate groundwater recharge, soil erosion, and baseflow in the Haean-myeon watershed in South Korea. The results show that the reduction of watershed slope increased groundwater recharge and baseflow, and decreased sediment. Specifically, groundwater recharge rate was increased from 257.10 to 364.60 mm, baseflow was increased from 0.86 to 1.19 m(3)/s, and sediment was decreased from 194.6 to 58.1 kg/km(2). Based on these results, the suggested method will positively contribute to aquatic ecosystems and farming environments in a steeply sloping watershed due to improvements in the quantity and quality of river water.

유량에 미치는 도시화 영향 평가

Journal of The Korean Society of Agricultural Engineers, 2014

Molecular cancer research : MCR, 2015

Annexin 2 (ANXA2) plays a critical role in hematopoietic stem cell (HSC) localization to the marr... more Annexin 2 (ANXA2) plays a critical role in hematopoietic stem cell (HSC) localization to the marrow niche. In part, ANXA2 supports HSCs by serving as an anchor for stromal-derived factor-1 (CXCL12/SDF-1). Recently, it was demonstrated that prostate cancer cells, like HSCs, use ANXA2 to establish metastases in marrow. The present study determined the capacity of ANXA2 expression by bone marrow stromal cells (BMSC) to facilitate tumor recruitment and growth through ANXA2-CXCL12 interactions. Significantly more CXCL12 was expressed by BMSC(Anxa2) (+/+) than by BMSC(Anxa2) (-/-) resulting in more prostate cancer cells migrating and binding to BMSC(Anxa2) (+/+) than BMSC(Anxa2) (-/-), and these activities were reduced when CXCL12 interactions were blocked. To further confirm that BMSC signaling through ANXA2-CXCL12 plays a critical role in tumor growth, immunocompromised SCID mice were subcutaneously implanted with human prostate cancer cells mixed with BMSC(Anxa2) (+/+) or BMSC(Anxa2) (...

유산양 유즙으로부터 분리된 세균의 분포 및 항균제 감수성 검사

Korean Journal of Veterinary Service, 2012

Lactoferrin protects against prion protein-induced cell death in neuronal cells by preventing mitochondrial dysfunction

International journal of molecular medicine, 2013

Prion disorder-related neurodegenerative diseases are characterized by the accumulation of prion ... more Prion disorder-related neurodegenerative diseases are characterized by the accumulation of prion protein (PrP) scrapie isoform (PrPsc) within the central nervous system. PrPsc induces neuronal cell death by increasing intracellular generation of reactive oxygen species (ROS). Lactoferrin (LF) is an 80 kDa protein, which has antioxidant abilities due to the scavenging of ROS. The effects of LF treatment on PrP (106-126)-mediated neurotoxicity and ROS generation were the focus of this study. LF treatment protected against PrP (106-126)-induced neuronal cell death and decreased ROS generation. The reduced ROS generation prevented PrP (106-126)-induced mitochondrial dysfunction. Moreover, PrP (106-126)-induced protein activation including c-Jun N-terminal kinase and caspase-3 were blocked by LF treatment. These results demonstrated that LF protects neuronal cells against PrP (106-126)-mediated neurotoxicity through the scavenging of ROS and provide evidence that LF treatment prevents ne...

stem cell adhesion, homing, and engraftment following transplantation Annexin II expressed by osteoblasts and endothelial cells regulates

Differentiation of hematopoietic stem cells (HSCs) after birth is largely restricted to the bone ... more Differentiation of hematopoietic stem cells (HSCs) after birth is largely restricted to the bone marrow cavity, where HSCs are associatedcloselywithosteoblasts(OBs). How OBs localize HSCs to the endosteal niche remains unclear. To explore adhe- sive interactions between HSCs and OBs, a cell blot analysis was used that re- vealed 2 major bands that corresponded to monomers and multimers of annexin II

Corbicula fluminea (Bivalvia: Corbiculidae): a possible second molluscan intermediate host of Echinostoma cinetorchis (Trematoda: Echinostomatidae) in Korea

The Korean journal of parasitology, 2001

More than 1,500 clams of Corbicula fluminea, the most favorable food source of freshwater bivalve... more More than 1,500 clams of Corbicula fluminea, the most favorable food source of freshwater bivalves in Korea, were collected from 5 localities to examine cercarial and metacercarial infection with Echinostoma cinetorchis. Although 3 clams infected with suspicious E. cinetorchis metacercariae out of 200 specimens collected at Kangjin, Chollanam-do were detected, no cercarial and metacercarial infections with E. cinetorchis were observed in field-collected Corbicula specimens. In the susceptibility experiments with laboratory-reared clams, those infected with miracidia of E. cinetorchis did not release their cercariae up to 60 days after infection. To confirm the identity of second intermediate host of E. cinetorchis experimentally, a total of 30 clams were exposed to the cercariae from Segmentina hemisphaerula that had been infected with miracidia of E. cinetorchis. The clams were susceptible to cercariae of E. cinetorchis with an infection rate of 93.3%. Metacercariae from clams take...