Juscivete Favero - Academia.edu (original) (raw)

Papers by Juscivete Favero

Microbial Pathogenesis, Sep 1, 2017

Bovine brucellosis is an important zoonotic disease caused by the bacterium Brucella abortus that... more Bovine brucellosis is an important zoonotic disease caused by the bacterium Brucella abortus that leads to economic losses due to animal discard and commercial restrictions. Since positive animals for brucellosis are culled, little is known about the pathogenesis of this disease. Therefore, the aims of this study were to evaluate possible changes in the activity of deaminase adenosine (ADA) and the oxidative stress in cows seropositives for brucellosis (Experiment I), and to evaluate the seroprevalence of B. abortus in dairy cows from the Western state of Santa Catarina, Southern Brazil (Experiment II). The Experiment I evaluated 20 pregnant cows: ten seropositives for B. abortus and ten seronegatives that were used as controls. The ADA activity and markers of oxidative stress (TBARS, catalase (CAT) and superoxide dismutase (SOD)) were evaluated in these animals. A reduction in the activity of ADA and catalase enzymes in seropositive animals was observed (p<0.001). Conversely, there was an increase in TBARS levels and superoxide dismutase activity in cows infected by B. abortus (p<0.001). The presence of oxidative stress and a reduction of ADA might be related to the modulation of the inflammatory response. The experiment II was performed due to a high number of herds with restrictions imposed by cases of brucellosis in the state of Santa Catarina in the last two years, and thus, the seroprevalence for B. abortus was evaluated in 1,242 serum samples of cows of 69 herds. The serodiagnosis was performed using two tests: buffered acidified antigen and 2-mercaptoethanol. However, none of the serum samples were positive for B. abortus. Although we did not find seropositive animals for brucellosis in our study, the disease still requires continued surveillance, due to its economic impact, and to the oxidative stress caused by it, which may have contributed to cases of abortion in three seropositive cows (Experiment I) in the final third of the gestation.

Microbial Pathogenesis, Jun 1, 2017

Leptospirosis is a cosmopolitan infectious disease that causes severe reproductive disorders in c... more Leptospirosis is a cosmopolitan infectious disease that causes severe reproductive disorders in cattle, especially those related to abortion. This disease has rodents as main reservoirs; however, cattle are responsible for maintenance of the disease. Thus, the aim of this study was to identify the factors associated with infection and cause-effect relation of leptospirosis in dairy herds from Southern of Brazil. Serum samples of 1242 cows were collected from herds classified as of medium and high density, and tested by microscopic agglutination test (MAT). These farms were located in the West part of Santa Catarina State (Brazil). A total of 80 cows (6.44%) were considered positives for the infection with titration of 1:100. Using a multivariate analysis, we identified two factors associated to bovine leptospirosis: dog access to pastures (p < 0.001) and feed exposure to rodents (p ¼ 0.05). Cause-effect analysis demonstrated that the occurrence of reproductive disorders was significantly (p ¼ 0.01) linked to leptospirosis. Thus, we conclude that leptospirosis is prevalent in dairy cattle in the west part of Santa Catarina state, as well as the access of dogs to pastures and contact of rodents with feed increase the chance of cattle infection by Leptospira spp.

Toxicological & Environmental Chemistry, May 16, 2017

The aim of this study was to evaluate nucleoside triphosphate diphosphohydrolase, 5'-nucleotidase... more The aim of this study was to evaluate nucleoside triphosphate diphosphohydrolase, 5'-nucleotidase, and adenosine deaminase activities in cattle fed on cuttings from pastures fertilized with urea compared to animals fed on cuttings from organically fertilized or from non-fertilized pastures. Blood samples were collected up to 9 h after feeding, and nitrite and enzyme activities were evaluated. The animals feeding on urea-fertilized pasture suffered subclinical intoxication. Serum nitrite levels increased by about 50 % and adenosine deaminase activity increased almost 6-fold 4 and 6 h after 3 feeding on urea-fertilized pasture, and adenosine deaminase in platelets almost doubled 6 and 9 h after feeding. In platelets, nucleoside triphosphate diphosphohydrolase activity with adenosine triphosphate as substrate increased 2, 4, 6 and 9 h after feeding while nucleoside triphosphate diphosphohydrolase with adenosine diphosphate as substrate and 5'-nucleotidase activities increased only 9 h after feeding. Increased activities of these enzymes may be implicated in the pathophysiology of subclinical intoxication since they are involved in many biochemical functions.

Comparative clinical pathology, Dec 21, 2015

The aim of this study was to report the Trypanosoma vivax in goats from a property located in the... more The aim of this study was to report the Trypanosoma vivax in goats from a property located in the west of the state of Santa Catarina, Brazil. The identification of the parasite occurred at random, that is, it was displayed a similar morphological trypomastigote form in the blood smear of an animal in the property. Subsequently, in blood samples collected from five goats on the same day, it was confirmed by polymerase chain reaction (PCR) the presence of the parasite in three of these animals. Being that by chance, consistent clinical signs related to trypanosomosis in these animals were not observed. The animals were not subjected to therapeutic treatment, because the molecular confirmation of the parasitism happened 1.4 years after the sample collection. Therefore, the T. vivax parasite infects goats in the state of Santa Catarina, Brazil.

Journal of Animal Physiology and Animal Nutrition, Aug 1, 2018

The aim of this study was to evaluate the behaviour of adenosine deaminase (ADA) activity, as wel... more The aim of this study was to evaluate the behaviour of adenosine deaminase (ADA) activity, as well as its participation in the immunomodulation of pregnant cows. Thus, sixteen cows were divided into two groups (A and B): the group A was composed by cows not pregnant (n = 8), while the group B was composed by pregnant cows (n = 8). Serum levels of interleukin-10 (IL-10), IL-6, tumour necrosis factor alpha (TNFα), reactive oxygen species (ROS) and C-reactive protein (CRP), as well as ADA and glutathione S-transferase (GST) activities, were measured on five sampling times (3, 5, 7 and 8 months of gestation, and soon after calving). Serum ADA activity was similar How to cite this article: Fávero JF, Da Silva AS, Bottari NB, et al. Physiological changes in the adenosine deaminase activity, antioxidant and inflammatory parameters in pregnant cows and at post-partum.

Pubvet, Apr 1, 2022

Resumo. A criação de bezerras é a base da produção leiteira, uma vez que há a necessidade de obte... more Resumo. A criação de bezerras é a base da produção leiteira, uma vez que há a necessidade de obter animais de qualidade para reposição do plantel. Baseando-se nos custos de criação e tendo como objetivo a manutenção do plantel, busca-se otimizar a criação e buscar o máximo de eficiência. Acompanhou-se a recria de bezerras em uma propriedade no interior do município de Nova Erechim, Santa Catarina, que trabalha com recria terceirizada, com objetivo de avaliar o crescimento em altura e peso. Permanecem alojados, a cada mês na propriedade, uma média de 200 animais com idade entre 10 dias a 9 meses. A alimentação ofertada varia nas diferentes fases de crescimento, sendo que a tomada do colostro é realizada na propriedade de origem. Foram avaliadas nove bezerras num período de três meses, totalizando 4 pesagens e mensuração de altura. Na segunda visita incluiu-se ao grupo estudado 11 bezerras, as quais foram acompanhadas durante 3 visitas, totalizando um lote de 20 bezerras assistidas. As bezerras em geral obtiveram um ganho de peso de 0,750 kg/dia, permanecendo dentro dos padrões descritos na literatura, já sobre a altura, os animais obtiveram um crescimento de 0,25 cm por dia, atingindo 100 cm aos 4 meses de idade, acima dos valores de referência.

Brazilian Journal of Development, Apr 23, 2021

Na bovinocultura de leite, a papilomatose bovina é uma das principais enfermidades que acometem o... more Na bovinocultura de leite, a papilomatose bovina é uma das principais enfermidades que acometem os animais, sendo causada por um herpes vírus da família Papillomaviridae. Os principais sinais clínicos incluem desenvolvimento de papilomas cutâneos em formato de couve-flor na região da face e pescoço, principalmente próximo aos olhos, desencadeando desconforto ao animal, prejudicando a produtividade. Como uma alternativa ao uso de moléculas sintéticas no tratamento da enfermidade a autohemoterapia foi aplicada neste estudo afim de avaliar sua eficácia na papilomatose bovina. O método utilizado para realizar a avaliação dos papilomas foi através de escore, sendo possível observar a evolução do tratamento realizado, comparando com início do tratamento. Para tal, os escores foram determinados em uma escala de 0 a 4 considerando o percentual de regressão dos papilomas: sem regressão de papilomas 0% (escore 0), regressão parcial até 50% (escore 2), regressão parcial acima de 50% (escore 3) e regressão total 100% (escore 4). O protocolo utilizado para o tratamento foi baseado na utilização de auto-hemoterapia associada a terapia medicamentosa com clorobutanol (Verruclin ®) e suplemento mineral (Fosfosal ®), durante o período de 35 dias. O estudo proporcionou resultados que permitiram avaliar de forma satisfatória o protocolo

Editora Conhecimento Livre eBooks, 2020

Comparative clinical pathology, Feb 6, 2017

The cholinesterases are enzymes involved in severe biological functions in mammals; however, it w... more The cholinesterases are enzymes involved in severe biological functions in mammals; however, it was not yet study in dairy cows during the transition period. In this period, the animals have high energetic demands for milk production, and the supplementation with mineral can be considered an important approach to fulfill this demand. Thus, the aim of this study was to evaluate the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in dairy cows supplemented with minerals based on selenium, copper, phosphorus, potassium, and magnesium intramuscularly in the transition period. The mineral supplementation in cows stimulates the increase of AChE postpartum (on days 2 and 7 postpartum, p < 0.05), as well as stimulates the BChE before and after the partum (on day 5 prepartum, and day 2 and 7 postpartum, p < 0.05). Calves of dairy cows supplemented showed low AChE activity when compared to calves of non-supplemented dairy cows. Thus, the supplementation with mineral did influence participates indirectly or directly especially in the modulation of cholinesterases that may have anti-inflammatory effect and contribute to improve on hematopoiesis and neurotransmission.

Comparative clinical pathology, Apr 18, 2017

Infections caused by protozoa often cause diarrhea in newborn calves, leading to high economic lo... more Infections caused by protozoa often cause diarrhea in newborn calves, leading to high economic losses to dairy farms, diarrhea, often characterized by malabsorption of nutrients, may lead to malnutrition, weight loss, and high mortality rates. Diarrhea is a common clinical sign found in the first days of a calf life in many dairy and beef cattle farms. Therefore, the aim of our study was to verify if during the first 60 days of live, there was a relationship between the presence of Eimeria, Cryptosporidium, Giardia, and the occurrence of diarrhea in calves, and the association of age and parasite presence. In this study, newborn calves were selected from farms with history of protozoa infections and diarrhea. A total of 26 calves were used in the study, 18 were positives for Giardia spp., 17 for Emeria spp., and 21 for Cryptosporidium spp., considering single and mixed infections. Furthermore, the survival rate of calves due to the presence of diarrhea was significantly different compared to the group without diarrhea, for all three protozoa. In addition, according to this study, it is possible to indicate that the occurrence of diarrhea is highly correlated to the presence of Giardia sp., leading to economic losses due to a low performance and weight gain of infected animals.

Revista Mvz Cordoba, May 2, 2017

Objective. This study aimed to evaluate the occurrence of gastrointestinal protozoa in dairy calv... more Objective. This study aimed to evaluate the occurrence of gastrointestinal protozoa in dairy calves and to identify potential risk factors for this type of infection. Materials and methods. For this purpose, 243 fecal samples were collected from calves up to 60 days of age in 43 dairy farms located in the West region of Santa Catarina state, Brazil. Samples were examined by centrifugal-flotation technique. Results. As a result, Giardia was present in 26.75% (65/243) of all samples, Eimeria in 21.81% (53/243), and Cryptosporidium in 20.99% (51/243). Additionally, 46.50% (113/243) of the samples were negative for any protozoa, while 39.10% (95/243) and 14.40% (35/208) showed single and mixed infections, respectively. There was a higher association between Cryptosporidium and Giardia (6.99%) in cases of mixed infections. However, the triple protozoa association had the lowest prevalence in mixed infections (2.06%). Epidemiologically, a questionnaire was applied to determine risk factors for these parasitic infections. Based on the statistical model applied, some risk factors for Cryptosporidium infections were identified, highlighting feeding management, period of time that calves stayed with their mothers (cows), and contact with dogs; the risk of contracting Giardia increased according to the milk source, while the floor type bedding, and age were appointed as risks factors for Eimeria. Conclusions. Therefore, it is possible to confirm that Giardia, Cryptosporidium and Eimeria may infect dairy calves, and the knowledge of some risk factors associated to their infection in calves.

Comparative clinical pathology, Nov 24, 2016

The dairy cattle production rises worldwide, with genetically selected animals. Consequently, the... more The dairy cattle production rises worldwide, with genetically selected animals. Consequently, they present high nutritional requirements, especially during the transition period. The aim of this study was to evaluate the effects of parenteral supplementation of a mineral blend composed by selenium, copper, magnesium, phosphorus, and potassium on hematological, biochemical, immunological, and antioxidant status in transition period dairy cows and newborn calves. The Holstein cows were divided into the control group (A, n = 3) and treated group (B, n = 4), which were supplemented twice with an intramuscularly dose of 10 mL/animal, at approximately 20 days prior to the parturition, and a subsequent dose at the parturition. Total blood was collected from cows on days 20 and 5 antepartum, and 2 and 7 postpartum, and from calves, at the birth. We observed a decreased in concentrations of thiobarbituric acid reactive substances (TBARSs) for cows fed mineral blend during the transition period (P < 0.05), as well as a greater number of total leukocyte, total protein and globulin levels, and reduction of hemoglobin levels (P < 0.05). The catalase activity was increased in supplemented cows and in its calves (P < 0.05). Therefore, based on these evidences, the parenteral supplementation with mineral provides beneficial effects in pregnant cows and during the transition period, as well as in their calves.

Animal Feed Science and Technology

DOAJ (DOAJ: Directory of Open Access Journals), Nov 1, 2021

Resumo. O objetivo do estudo foi o isolamento e a identificação dos principais microrganismos cau... more Resumo. O objetivo do estudo foi o isolamento e a identificação dos principais microrganismos causadores de mastite clínica e subclínica em vacas leiteiras da região Oeste de Santa Catarina e a relação de sensibilidade e resistência dos patógenos encontrados pelo método de antibiograma, frente aos antimicrobianos frequentemente utilizados nos tratamentos. Foram utilizadas 26 amostras de leite de bovinos que apresentavam a enfermidade mastite, com distinção de raça, idade e peso, provenientes de pequenas, médias e grandes propriedades. A mastite clínica foi identificada pelos sinais clínicos de edema, vermelhidão, aumento de temperatura, endurecimento e dor na glândula mamária e a mastite subclínica foi evidenciada pelo California Mastitis Test (CMT) e aumento da Contagem de Células Somáticas (CCS). Foram identificados 13 microrganismos, sendo 12 (92%) bactérias, sendo o maior índice de isolamentos pertencentes ao grupo dos Staphylococcus ssp, Streptococcus ssp e Escherichia coli e uma (8%) amostra de levedura. O antibiograma, identificou as drogas com perfil de sensibilidade mais satisfatórias, sendo Amoxicilina, Ampicilina e Gentamicina com 83% das amostras demonstrando sensibilidade e a resistência de 58% das amostras frentes a droga Oxacilina. O presente estudo, possibilitou o levantamento epidemiológico e contribuição para selecionar o tratamento eficaz, com redução da resistência aos antibióticos.

Ensino, pesquisa e extensão no Brasil: uma abordagem pluralista, 2020

Chemischer Informationsdienst, 1979

Inkubation der 9β,10α‐Steroide (I) mit Rhizopus arrhizus Fischer CBS 127.08 (R. arr.) führt überw... more Inkubation der 9β,10α‐Steroide (I) mit Rhizopus arrhizus Fischer CBS 127.08 (R. arr.) führt überwiegend zu 9β‐Hydroxy‐Derivaten: Aus (Ia) entsteht (II) im Gemisch mit Polyhydroxyabkömmlingen; (Ib) liefert zwei Metaboliten, aus deren Gemisch erst nach Acetylierung die höher polare Komponente als Acetat (III) isoliert werden kann; neben dem erwarteten Alkohol (IVa) erhält man aus (Ic) auch das 6β‐Isomere (IVb).

Chemischer Informationsdienst, 1978

Bei der Inkubation von 19‐Nortestosteron (I) mit einer Kultur von Rhizopus arrhizus (R. arr.) tri... more Bei der Inkubation von 19‐Nortestosteron (I) mit einer Kultur von Rhizopus arrhizus (R. arr.) tritt stereospezifisch Hydroxylierung am C‐10 und C‐6 ein, wobei ein untrennbares 60 z40‐Gemisch der isomeren Hydroxynortestosterone (IIa) und (IIb) gebildet wird.

Comparative Clinical Pathology, 2017

Toxoplasma gondii is responsible for reproductive failure in sheep, and it is considered a zoonot... more Toxoplasma gondii is responsible for reproductive failure in sheep, and it is considered a zoonotic parasite. Therefore, the aim of this study was to evaluate the prevalence of toxoplasmosis and its risk factors at the farm level. For that, 314 sheep from 18 farms of 7 municipalities of the West part of Santa Catarina State, Southern Brazil were used. To identify possible risk factors associated to the disease, a survey was applied to farmers with questions regarding farm characteristics, type of management, and history of animal reproductive problems. Out of 314 sera samples, 118 (37%, CI 95% 32.3-43.3) were positives for T. gondii. Multivariate analyses showed that allowing cats to have access to sheep feed was significantly associated to toxoplasmosis, with an odds ratio of 2.42. Similarly, keeping cats in the farm increased 1.67 times the chances of T. gondii infection by sheep. Therefore, we concluded that sheep infection by T. gondii in the West of Santa Catarina state is distributed very similarly to other regions of Brazil, and the presence of cats in the property is strongly correlated to the occurrence of the disease.

Revista MVZ Córdoba, 2016

Objective. The aim of this study was to investigate the relation among type and size of the farms... more Objective. The aim of this study was to investigate the relation among type and size of the farms related to the management of production and especially the presence and control of parasites. Materials and methods. To accomplish the above, 65 farms were used: 56 of semi-extensive system (cows in pastures), classified as low density farms (n=15, up to 15/cows), medium density farms (n=20; between 16-30/cows) and large density farms (n=21; over 31/cows). It was also included nine farms freestall system (n=9; confinement, over 70 cows) as part of the study population. Results. In the farms visited for the study can be noticed that the farms that had the higher level of technological improvement in managements practice were properties in high level (3) and freestall (4). In most of the farms there were observed the presence of ticks and flies, regardless of density of farms. Feces samples collected from 650 cows (n=10/farm), and analyzed using McMaster showed that 191 cows were positive...