Kátia Souto - Academia.edu (original) (raw)

Papers by Kátia Souto

Dedico este trabalho às minhas filhas Aline, Ana Clara e Érica por serem a minha fonte de estímul... more Dedico este trabalho às minhas filhas Aline, Ana Clara e Érica por serem a minha fonte de estímulo constante e também ao meu esposo Paulo Roberto pelo carinho, amor e paciência demonstrados em todos os momentos. VI Agradecimentos ___________________________________________________________ Primeiro agradeço a Deus pelo dom da vida e pelo grande privilégio de estar neste momento finalizando este estudo. Agradeço à minha família, meu pai Geraldo e minha mãe Vera Maria, que sempre me apoiaram desde a infância, estimulando o estudo. Ao meu irmão, Paulo Henrique pela generosidade e apoio em todos os momentos de minha vida. Agradeço à minha irmã caçula Profa. Dra. Andréa Damin, médica que me serve de exemplo, cuja dedicação incansável ao estudo é um estímulo para todos os que dela se aproximam. Ao meu orientador Professor Dr. Daniel de Carvalho Damin por ter acreditado no meu trabalho e por compartilhar sua vivacidade, inteligência e gosto pelo trabalho científico, até a finalização deste estudo. Agradeço especialmente à professora Dra. Jane Maria Ulbrich-Kulkzinsky pelo imenso trabalho com revisão das lâminas e classificação. Agradeço em especial à funcionária da patologia Fernanda Xavier do Hospital Conceição por ter buscado lâminas e material de biópsias hepáticas para que pudessem ser avaliadas. Também agradeço especialmente à Dra. Denise Blaya Rocha pelo apoio incondicional, principalmente nos momentos difíceis desta jornada. Minha gratidão vai também para Isabella Dossin na época ainda estudante de medicina pelo auxílio na coleta de dados. Aos colegas do Serviço de

Diabetic Medicine, Dec 1, 2005

Aim To compare the frequencies of the G1888A variant in the mitochondrial 16S rRNA gene between ... more Aim To compare the frequencies of the G1888A variant in the mitochondrial 16S rRNA gene between patients with Type 2 diabetes and non‐diabetic control subjects from southern Brazil.Methods We analysed 520 Type 2 diabetic patients (389 Caucasian– and 131 African–Brazilians) and 530 control subjects (400 Caucasian– and 130 African–Brazilians). DNA samples were amplified by polymerase chain reaction and digested with the RsaI enzyme. Variant frequency in patients and control subjects was compared using χ2 test, Fisher's exact test or odds ratio test. We also investigated the frequency of the G1888A variant in a subgroup of the patients with a maternal history of Type 2 diabetes plus two or more features of maternally inherited diabetes and deafness.Results The 1888A allele does not seem to be associated with Type 2 diabetes in African–Brazilians (frequency of 3.8% in patients and 0.8% in control subjects; PFisher = 0.213). However, in Caucasian–Brazilians, the 1888A allele was significantly associated with diabetes (12.3% in patients vs. 3.5% in control subjects; OR = 3.881; 95% CI 2.106–7.164; P < 0.001) and also with higher levels of insulin resistance. The majority of the patients carrying the 1888A allele did not have clinical features of maternally inherited diabetes and deafness.Conclusion The present study indicates the association of the mitochondrial G1888A variant with Type 2 diabetes and insulin resistance in Caucasian–Brazilian patients from southern Brazil. However, further studies are required to confirm its effects on mitochondrial function and the role of these effects on the pathogenesis of Type 2 diabetes.

PubMed, Dec 17, 2005

Background: In this case-control study, we investigated the possible involvement of the p22phox C... more Background: In this case-control study, we investigated the possible involvement of the p22phox C242T polymorphism in the development and progression of diabetic nephropathy (DN) in 535 Caucasian Brazilians with type 2 diabetes. We also evaluated the effects of the interaction of the C242T polymorphism with smoking and hypercholesterolemia on the susceptibility to nephropathy. Methods: Genotype analysis was performed using polymerase chain reaction (PCR) followed by digestion with restriction enzyme. Logistic regression analysis was used to control for independent risk factors associated with nephropathy. Results: The genotype frequencies in patients with overt DN (CC/CT/TT: 0.36/0.47/0.17) were not significantly different from those of diabetic individuals with normoalbuminuria (0.47/0.41/0.12) or microalbuminuria (0.42/0.48/0.10) (p=0.214). Likewise, there were no differences in the T allele frequency among patients with normoalbuminuria, microalbuminuria or overt DN (0.33, 0.34 and 0.40, respectively; p=0.111). However, the T allele was found to be more frequent among smokers with overt nephropathy (macroalbuminuria and/or in dialysis) than those who had normoalbuminuria (43 vs. 32%, p=0.045). The multiple logistic regression analysis confirmed that the CT+TT genotypes were independently associated with a higher risk of having overt nephropathy among smokers [odds ratio (OR)=6.76, 95% confidence interval (95% CI) 1.83-25.02]. Conclusions: Our study shows a gene-environment interaction associated with the increased risk of DN progression in Caucasian Brazilian smokers with type 2 diabetes. Further studies should be performed to clarify whether it exists, and to what extent there is a relationship between the p22phox C242T polymorphism and DN.

Disease Markers, 2006

Catalase is a central antioxidant enzyme constituting the primary defense against oxidative stres... more Catalase is a central antioxidant enzyme constituting the primary defense against oxidative stress. In this study, we investigated whether the functional-262C/T polymorphism in the promoter of catalase gene is associated with the presence of diabetic retinopathy (DR), diabetic nephropathy (DN) and ischemic heart disease (IHD) in 520 Caucasian-Brazilians with type 2 diabetes. The-262C/T polymorphism was also examined in 100 Caucasian blood donors. Patients underwent a clinical and laboratory evaluation consisting of a questionnaire, physical examination, assessment of diabetic complications and laboratory tests. Genotype analysis was performed using the polymerase chain reaction followed by digestion with restriction enzyme. The genotype and allele frequencies of the-262C/T polymorphism in patients with type 2 diabetes were very similar to those of blood donors (T allele frequency = 0.20 and 0.18, respectively). Likewise, there were no differences in either genotype or allele frequencies between type 2 diabetic patients with or without DR, DN or IHD. Thus, our results do not support the hypothesis that the-262C/T polymorphism is related to the development of DR, DN or IHD in patients with type 2 diabetes. Further studies are necessary to elucidate the role of catalase gene polymorphisms in the pathogenesis of diabetic complications.

Molecular Genetics and Metabolism, Jul 1, 2006

Diabetic retinopathy is a sight-threatening chronic complication of diabetes mellitus and is the ... more Diabetic retinopathy is a sight-threatening chronic complication of diabetes mellitus and is the leading cause of acquired blindness in adults. The ¡106C > T polymorphism in the promoter region of the aldose reductase (AR) gene has been shown to be associated with the susceptibility to diabetic nephropathy in type 2 diabetes, but the Wndings regarding the occurrence of diabetic retinopathy are conXicting. In this case-control study, we investigated whether the ¡106C > T polymorphism in the AR gene is involved in the development and progression of diabetic retinopathy in 579 Brazilians with type 2 diabetes (424 Caucasian-and 155 African-Brazilians). Patients underwent a clinical and laboratory evaluation consisting of a questionnaire, physical examination, assessment of diabetic complications and laboratory tests. Genotype analysis was performed using the polymerase chain reaction followed by digestion with restriction enzyme. Logistic regression analysis was used to control for independent risk factors associated with diabetic retinopathy. There were no diVerences in either genotype or allele frequencies for the ¡106C > T polymorphism between type 2 diabetic patients with or without diabetic retinopathy, in both ethnic groups. However, the CC genotype was associated with an increased risk of having proliferative diabetic retinopathy in Caucasian-Brazilians with type 2 diabetes (odds ratio (OR) D 2.04; 95% conWdence interval (CI) D 1.21-3.45; P D 0.007), independently of other risk factors associated with this complication. Thus, our results show that the ¡106CC genotype (¡106C > T polymorphism) in the AR gene is related to the progression of diabetic retinopathy in Caucasian-Brazilians with type 2 diabetes.

Annals of Human Genetics, Jul 1, 2006

The aims of this study were to investigate the contributions of the mitochondrial DNA m.4216T > C... more The aims of this study were to investigate the contributions of the mitochondrial DNA m.4216T > C and m.4917A > G variants, and also of the European-specific mitochondrial cluster J/T, to the development of type 2 diabetes mellitus in Caucasian-Brazilian patients from Southern Brazil. We analyzed 347 type 2 diabetes patients and 350 control subjects. Variant frequencies in patients and control subjects were compared using χ 2 tests or odds ratio. We also compared clinical and laboratory characteristics among patients with and without the variants. We found that the frequencies of the m.4216T > C and m.4917A > G variants are higher in diabetic patients than in control subjects. Moreover, haplogroups J (partially defined by the presence of the m.4216T > C variant only) and T (partially defined by the presence of both m.4216T > C and m.4917A > G variants) are more frequent in the type 2 diabetic group than in the control group. Patients belonging to the cluster J/T are more insulin resistant than patients of other haplogroups. In conclusion, our results indicate the association of the cluster J/T (as suggested by analyses of the m.4216T > C and m.4917A > G variants) with insulin resistance and type 2 diabetes.

Diabetes Care, Feb 1, 2007

OBJECTIVE-The purpose of this study was to evaluate the effect of the single nucleotide polymorph... more OBJECTIVE-The purpose of this study was to evaluate the effect of the single nucleotide polymorphism (SNP) Ϫ634GϾC at the 5Ј regulatory region of the vascular endothelial growth factor (VEGF) in the risk of proliferative diabetic retinopathy (PDR) in the Brazilian population of European ancestry with type 2 diabetes. RESEARCH DESIGN AND METHODS-A case-control study was conducted in 501 type 2 diabetic patients of European ancestry. Patients underwent a standardized clinical, ophthalmological, and laboratory evaluation. Of these, 167 patients had PDR (case patients), and 334 were considered as control subjects (patients without PDR) for PDR. A reference population (110 individuals of European ancestry) was also evaluated. RESULTS-No evidence of association between Ϫ634GϾC/VEGF and the presence of diabetic retinopathy or type 2 diabetes was observed (P Ͼ 0.05). However, CC homozygous for the SNP Ϫ634GϾC was significantly more frequent in patients with PDR (37 of 167; 22.2%) than in the corresponding control group (40 of 334; 12%) in accordance with a recessive model (P ϭ 0.003). This effect was further observed when creatinine, BMI, sex, duration of type 2 diabetes, HDL cholesterol, and systolic blood pressure were taken into account (odds ratio 1.9 [95% CI 1.01-3.79], P ϭ 0.04). CONCLUSIONS-The presence of the allele Ϫ634C/VEGF in homozygosity is an independent risk factor for the development of PDR in type 2 diabetic patients of European ancestry.

Molecular Genetics and Metabolism, Jun 1, 2005

Annals of Human Genetics, Jan 17, 2006

Clinical Endocrinology, Jul 1, 2002

Arquivos Brasileiros De Endocrinologia E Metabologia, Oct 1, 2006

Objective: To investigate the presence of maternal and paternal history of type 2 diabetes mellit... more Objective: To investigate the presence of maternal and paternal history of type 2 diabetes mellitus (DM) in relatives of 644 type 2 diabetic patients from Southern Brazil, and also to evaluate its influence on the clinical characteristics of this disease. Patients and Methods: Familial history of type 2 DM was investigated by a questionnaire. The maternal and paternal history was investigated over two generations. Complete data sets on familial history were obtained from 396 patients. Results: In general, 76.6% of the patients reported at least one first-degree affected relative. Besides, 31.6% of the patients reported a maternal history of type 2 DM and 12.6% reported a paternal history. Patients with maternal and/or paternal history presented a lower age at type 2 DM diagnosis when compared to patients without familial history. In addition, patients with only paternal history presented a higher frequency of hypertension than patients with no familial history. Conclusions: This study suggests that there is a significant maternal effect in the transmission of type 2 DM in Southern Brazil, and that most of the clinical characteristics of this disease do not differ between patients with or without familial history of type 2 DM.

Entrevista realizada com Fernando Pigatto, atual presidente do Conselho Nacional de Saúde (CNS), ... more Entrevista realizada com Fernando Pigatto, atual presidente do Conselho Nacional de Saúde (CNS), por via remota, em 25/06/2021, quando o País chegava a 500.000 mortos pela pandemia de Covid-19. Nela, os entrevistadores objetivaram, por meio de perguntas abertas, levantar informações que (i) apresentassem a atuação do CNS no contexto pandêmico, em especial seus desafios e avanços; e (ii) promovessem a reflexão do entrevistado sobre aspectos fundamentais da democracia e da participação social, sobretudo a relação do Conselho com a sociedade civil e a sociedade política, o papel do CNS no processo decisório das políticas de saúde (ator e/ou arena?) e a interação Conselho-Conferência.

Clinical Gastroenterology and Hepatology, 2004

BACKGROUND & AIMS Nonalcoholic fatty liver disease (NAFLD) is reported commonly in patients w... more BACKGROUND & AIMS Nonalcoholic fatty liver disease (NAFLD) is reported commonly in patients with type 2 diabetes mellitus (DM), which has been suggested as a risk factor for the progressive form of NAFLD, or nonalcoholic steatohepatitis. The aim of this study was to assess the outcome of patients with NAFLD and DM. METHODS A cohort of patients with NAFLD was identified, and patients with other causes of liver disease (alcohol, medication, etc.) were excluded. Clinical, pathological, and mortality data were available for this cohort. Patients were categorized and compared according to the presence or absence of DM. RESULTS Of 132 patients with NAFLD, 44 patients (33%) had an established diagnosis of DM. Patients with DM were older and had greater serum glucose and triglyceride levels and a greater aspartate aminotransferase-alanine aminotransferase ratio. Liver biopsy specimens from patients with DM showed more vacuolated nuclei and acidophilic bodies. Cirrhosis (histological or clinical) occurred in 25% of patients with DM (11 of 44 patients) and NAFLD compared with only 10.2% (9 of 88 patients) of patients without DM with NAFLD (P = 0.04). After adjusting for potential confounders (age, body mass index, and the presence of cirrhosis), both overall mortality (risk ratio [RR], 3.30; 95% confidence interval [CI], 1.76-6.18; P = 0.002) and mortality related to liver disease (RR, 22.83; 95% CI, 2.97-175.03; P = 0.003) were greater in diabetic patients with NAFLD. Markers of hepatic dysfunction (low albumin level, high total bilirubin level, and prolonged prothrombin time) were the only independent predictors of increased mortality. CONCLUSIONS Patients with NAFLD and DM are at risk for the development of an aggressive outcome, such as cirrhosis and mortality. This study supports the potential role of insulin resistance in the development of poor clinical outcomes in patients with NAFLD.

Annals of Human Genetics, 2006

SummaryThe aims of this study were to investigate the contributions of the mitochondrial DNA m.42... more SummaryThe aims of this study were to investigate the contributions of the mitochondrial DNA m.4216T > C and m.4917A > G variants, and also of the European‐specific mitochondrial cluster J/T, to the development of type 2 diabetes mellitus in Caucasian‐Brazilian patients from Southern Brazil. We analyzed 347 type 2 diabetes patients and 350 control subjects. Variant frequencies in patients and control subjects were compared using χ2 tests or odds ratio. We also compared clinical and laboratory characteristics among patients with and without the variants. We found that the frequencies of the m.4216T > C and m.4917A > G variants are higher in diabetic patients than in control subjects. Moreover, haplogroups J (partially defined by the presence of the m.4216T > C variant only) and T (partially defined by the presence of both m.4216T > C and m.4917A > G variants) are more frequent in the type 2 diabetic group than in the control group. Patients belonging to the clust...

Rev. psiquiatr. Rio …, 2002

Base de dados : LILACS. Pesquisa : 360301 [Identificador único]. Referências encontradas : 1 [ref... more Base de dados : LILACS. Pesquisa : 360301 [Identificador único]. Referências encontradas : 1 [refinar]. Mostrando: 1 .. 1 no formato [Detalhado]. página 1 de 1, 1 / 1, LILACS, seleciona. para imprimir. Fotocópia. experimental, Documentos relacionados. Id: 360301. ...

Ciências médicas: Campo teórico, métodos, aplicabilidade e limitações 2, 2021