Konstantinos Chandolias - Academia.edu (original) (raw)

Papers by Konstantinos Chandolias

Research paper thumbnail of Bio-Hydrogen and Vfa Production from Steel Mill Gases Using Pure and Mixed Bacterial Cultures

Research paper thumbnail of Bio-Hydrogen and Vfa Production from Steel Mill Gases Using Pure and Mixed Bacterial Cultures

Research paper thumbnail of Protective effect of a reverse membrane bioreactor against toluene and naphthalene in anaerobic digestion

Biotechnology and applied biochemistry, 2021

Raw syngas contains tar contaminants including toluene and naphthalene, which inhibit its convers... more Raw syngas contains tar contaminants including toluene and naphthalene, which inhibit its conversion to methane. Cell encasement in a hydrophilic reverse membrane bioreactor (RMBR) could protect the cells from hydrophobic contaminants. This study aimed to investigate the inhibition of toluene and naphthalene and the effect of using RMBR. In this work, toluene and napthalene were added at concentrations of 0.5 - 1.0 and 0.1 - 0.2 g/L in batch operation. In continuous operation, concentration of 0 - 6.44 g/L for toluene and 0 - 1.28 g/L for napthalene were studied. The results showed that no inhibition was observed in batch operation for toluene and naphthalene at concentrations up to 1 and 0.2 g/L, respectively. In continuous operation of free cell bioreactors (FCBR), inhibition of toluene and naphthalene started at 2.05 g/L and 0.63 g/L, respectively. When they were present simultaneously, inhibition of toluene and naphthalene occurred at concentrations of 3.14 g/L and 0.63 g/L, res...

Research paper thumbnail of Rapid Bio-methanation of Syngas by High Cell-density in Reverse Membrane Bioreactors

Syngas fermentation via gasification is a two-stage process, which contains gasification of feeds... more Syngas fermentation via gasification is a two-stage process, which contains gasification of feedstock into syngas and syngas bio-methanation by anaerobic microorganisms. This project is a study on ...

Research paper thumbnail of Lignocellulosic Biorefinery for Biohydrogen and Carboxylic Acids Production in Flexible Membrane Bioreactor and Two-stage System

Lignocellulosic biorefineries can produce numerous biofuels and chemicals via the anaerobic diges... more Lignocellulosic biorefineries can produce numerous biofuels and chemicals via the anaerobic digestion process. Although several works have been recently conducted on this field, the technology is c ...

Research paper thumbnail of Enhanced Methane and Hydrogen production in Reverse Membrane Bioreactors via Syngas Fermentation

of waste treatment processes, such as the anaerobic digestion. This biochemical process converts ... more of waste treatment processes, such as the anaerobic digestion. This biochemical process converts organic substrates into biogas, with anaerobic microorganisms. However, some types of substrates hav ...

Research paper thumbnail of Combined Gasification-Fermentation Process in Waste Biorefinery

Research paper thumbnail of Floating Membrane Bioreactors with High Gas Hold-Up for Syngas-to-Biomethane Conversion

Energies

The low gas-to-liquid mass transfer rate is one of the main challenges in syngas biomethanation. ... more The low gas-to-liquid mass transfer rate is one of the main challenges in syngas biomethanation. In this work, a new concept of the floating membrane system with high gas hold-up was introduced in order to enhance the mass transfer rate of the process. In addition, the effect of the inoculum-to-syngas ratio was investigated. The experiments were conducted at 55 °C with an anaerobic mixed culture in both batch and continuous modes. According to the results from the continuous experiments, the H2 and CO conversion rates in the floating membrane bioreactor were approximately 38% and 28% higher in comparison to the free (suspended) cell bioreactors. The doubling of the thickness of the membrane bed resulted in an increase of the conversion rates of H2 and CO by approximately 6% and 12%, respectively. The highest H2 and CO consumption rates and CH4 production rate recorded were approximately 22 mmol/(L·d), 50 mmol/(L·d), and 34.41 mmol/(L·d), respectively, obtained at the highest inoculu...

Research paper thumbnail of Effects of Heavy Metals and pH on the Conversion of Biomass to Hydrogen via Syngas Fermentation

BioResources

The effects of three heavy metals on hydrogen production via syngas fermentation were investigate... more The effects of three heavy metals on hydrogen production via syngas fermentation were investigated within a metal concentration range of 0 to 1.5 mg Cu/L, 0 to 9 mg Zn/L, 0 to 42 mg Mn/L, in media with initial pH of 5, 6, and 7, at 55 °C. The results showed that at lower metal concentration, pH 6 was optimum while at higher metal concentrations, pH 5 stimulated the process. More specifically, the highest hydrogen production activity recorded was 155% ± 12% at a metal concentration of 0.04 mg Cu/L, 0.25 mg Zn/L, and 1.06 mg Mn/L and an initial medium pH of 6. At higher metal concentration (0.625 mg Cu/L, 3.75 mg Zn/L, and 17.5 mg Mn/L), only pH 5 was stimulating for the cells. The results showed that the addition of heavy metals, contained in gasification-derived ash, can improve the production rate and yield of fermentative hydrogen. This could lead to lower costs in gasification process and fermentative hydrogen production and less demand for syngas cleaning before syngas fermentation.

Research paper thumbnail of Innovative Pretreatment Strategies for Biogas Production

Bioresource Technology, 2016

Research paper thumbnail of Biohydrogen and carboxylic acids production from wheat straw hydrolysate

Bioresource Technology, 2016

Research paper thumbnail of Rapid Bio-methanation of Syngas by High Cell-density in Reverse Membrane Bioreactors

iii Acknowledgements First of all, I would like to express my gratitude to Professor Mohammad Tah... more iii Acknowledgements First of all, I would like to express my gratitude to Professor Mohammad Taherzadeh for his guidelines and trust as well as my supervisor Dr. Supansa Youngsukkasem, who has been very supportive, committed and an excellent teacher. Moreover, I would like to thank the Biotechnology and Bioethanol group for their warm welcome and for assisting me in the lab and the staff of the School of Engineering for their professionalism, punctuality and accurate work. Last but not least, I thank my family for their love and support and especially Magda for her love, understanding and for filling me with positive energy.

Research paper thumbnail of Syngas Biomethanation in a Semi-Continuous Reverse Membrane Bioreactor (RMBR)

Fermentation, 2016

Syngas biomethanation is a potent bio-conversion route, utilizing microorganisms to assimilate in... more Syngas biomethanation is a potent bio-conversion route, utilizing microorganisms to assimilate intermediate gases to produce methane. However, since methanogens have a long doubling time, the reactor works best at a low dilution rate; otherwise, the cells can be washed out during the continuous fermentation process. In this study, the performance of a practical reverse membrane bioreactor (RMBR) with high cell density for rapid syngas biomethanation as well as a co-substrate of syngas and organic substances was examined in a long-term fermentation process of 154 days and compared with the reactors of the free cells (FCBR). The RMBR reached maximum capacities of H 2 , CO, and CO 2 conversion of 7.0, 15.2, and 4.0 mmol/L reactor .day, respectively, at the organic loading rate of 3.40 gCOD/L.day. The highest methane production rate from the RMBR was 186.0 mL/L reactor .day on the 147th day, compared to the highest rate in the FCBR, 106.3 mL/L reactor .day, on the 58th day. The RMBR had the ability to maintain a high methanation capacity by retaining the microbial cells, which were at a high risk for cell wash out. Consequently, the system was able to convert more syngas simultaneously with the organic compounds into methane compared to the FCBR.

Research paper thumbnail of Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms

Bioresource Technology, 2015

Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-metha... more Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms, Bioresource Technology (2014), doi: http:// dx.

Research paper thumbnail of Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms

Bioresource Technology, 2015

Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-metha... more Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms, Bioresource Technology (2014), doi: http:// dx.

Research paper thumbnail of Bio-Hydrogen and Vfa Production from Steel Mill Gases Using Pure and Mixed Bacterial Cultures

Research paper thumbnail of Bio-Hydrogen and Vfa Production from Steel Mill Gases Using Pure and Mixed Bacterial Cultures

Research paper thumbnail of Protective effect of a reverse membrane bioreactor against toluene and naphthalene in anaerobic digestion

Biotechnology and applied biochemistry, 2021

Raw syngas contains tar contaminants including toluene and naphthalene, which inhibit its convers... more Raw syngas contains tar contaminants including toluene and naphthalene, which inhibit its conversion to methane. Cell encasement in a hydrophilic reverse membrane bioreactor (RMBR) could protect the cells from hydrophobic contaminants. This study aimed to investigate the inhibition of toluene and naphthalene and the effect of using RMBR. In this work, toluene and napthalene were added at concentrations of 0.5 - 1.0 and 0.1 - 0.2 g/L in batch operation. In continuous operation, concentration of 0 - 6.44 g/L for toluene and 0 - 1.28 g/L for napthalene were studied. The results showed that no inhibition was observed in batch operation for toluene and naphthalene at concentrations up to 1 and 0.2 g/L, respectively. In continuous operation of free cell bioreactors (FCBR), inhibition of toluene and naphthalene started at 2.05 g/L and 0.63 g/L, respectively. When they were present simultaneously, inhibition of toluene and naphthalene occurred at concentrations of 3.14 g/L and 0.63 g/L, res...

Research paper thumbnail of Rapid Bio-methanation of Syngas by High Cell-density in Reverse Membrane Bioreactors

Syngas fermentation via gasification is a two-stage process, which contains gasification of feeds... more Syngas fermentation via gasification is a two-stage process, which contains gasification of feedstock into syngas and syngas bio-methanation by anaerobic microorganisms. This project is a study on ...

Research paper thumbnail of Lignocellulosic Biorefinery for Biohydrogen and Carboxylic Acids Production in Flexible Membrane Bioreactor and Two-stage System

Lignocellulosic biorefineries can produce numerous biofuels and chemicals via the anaerobic diges... more Lignocellulosic biorefineries can produce numerous biofuels and chemicals via the anaerobic digestion process. Although several works have been recently conducted on this field, the technology is c ...

Research paper thumbnail of Enhanced Methane and Hydrogen production in Reverse Membrane Bioreactors via Syngas Fermentation

of waste treatment processes, such as the anaerobic digestion. This biochemical process converts ... more of waste treatment processes, such as the anaerobic digestion. This biochemical process converts organic substrates into biogas, with anaerobic microorganisms. However, some types of substrates hav ...

Research paper thumbnail of Combined Gasification-Fermentation Process in Waste Biorefinery

Research paper thumbnail of Floating Membrane Bioreactors with High Gas Hold-Up for Syngas-to-Biomethane Conversion

Energies

The low gas-to-liquid mass transfer rate is one of the main challenges in syngas biomethanation. ... more The low gas-to-liquid mass transfer rate is one of the main challenges in syngas biomethanation. In this work, a new concept of the floating membrane system with high gas hold-up was introduced in order to enhance the mass transfer rate of the process. In addition, the effect of the inoculum-to-syngas ratio was investigated. The experiments were conducted at 55 °C with an anaerobic mixed culture in both batch and continuous modes. According to the results from the continuous experiments, the H2 and CO conversion rates in the floating membrane bioreactor were approximately 38% and 28% higher in comparison to the free (suspended) cell bioreactors. The doubling of the thickness of the membrane bed resulted in an increase of the conversion rates of H2 and CO by approximately 6% and 12%, respectively. The highest H2 and CO consumption rates and CH4 production rate recorded were approximately 22 mmol/(L·d), 50 mmol/(L·d), and 34.41 mmol/(L·d), respectively, obtained at the highest inoculu...

Research paper thumbnail of Effects of Heavy Metals and pH on the Conversion of Biomass to Hydrogen via Syngas Fermentation

BioResources

The effects of three heavy metals on hydrogen production via syngas fermentation were investigate... more The effects of three heavy metals on hydrogen production via syngas fermentation were investigated within a metal concentration range of 0 to 1.5 mg Cu/L, 0 to 9 mg Zn/L, 0 to 42 mg Mn/L, in media with initial pH of 5, 6, and 7, at 55 °C. The results showed that at lower metal concentration, pH 6 was optimum while at higher metal concentrations, pH 5 stimulated the process. More specifically, the highest hydrogen production activity recorded was 155% ± 12% at a metal concentration of 0.04 mg Cu/L, 0.25 mg Zn/L, and 1.06 mg Mn/L and an initial medium pH of 6. At higher metal concentration (0.625 mg Cu/L, 3.75 mg Zn/L, and 17.5 mg Mn/L), only pH 5 was stimulating for the cells. The results showed that the addition of heavy metals, contained in gasification-derived ash, can improve the production rate and yield of fermentative hydrogen. This could lead to lower costs in gasification process and fermentative hydrogen production and less demand for syngas cleaning before syngas fermentation.

Research paper thumbnail of Innovative Pretreatment Strategies for Biogas Production

Bioresource Technology, 2016

Research paper thumbnail of Biohydrogen and carboxylic acids production from wheat straw hydrolysate

Bioresource Technology, 2016

Research paper thumbnail of Rapid Bio-methanation of Syngas by High Cell-density in Reverse Membrane Bioreactors

iii Acknowledgements First of all, I would like to express my gratitude to Professor Mohammad Tah... more iii Acknowledgements First of all, I would like to express my gratitude to Professor Mohammad Taherzadeh for his guidelines and trust as well as my supervisor Dr. Supansa Youngsukkasem, who has been very supportive, committed and an excellent teacher. Moreover, I would like to thank the Biotechnology and Bioethanol group for their warm welcome and for assisting me in the lab and the staff of the School of Engineering for their professionalism, punctuality and accurate work. Last but not least, I thank my family for their love and support and especially Magda for her love, understanding and for filling me with positive energy.

Research paper thumbnail of Syngas Biomethanation in a Semi-Continuous Reverse Membrane Bioreactor (RMBR)

Fermentation, 2016

Syngas biomethanation is a potent bio-conversion route, utilizing microorganisms to assimilate in... more Syngas biomethanation is a potent bio-conversion route, utilizing microorganisms to assimilate intermediate gases to produce methane. However, since methanogens have a long doubling time, the reactor works best at a low dilution rate; otherwise, the cells can be washed out during the continuous fermentation process. In this study, the performance of a practical reverse membrane bioreactor (RMBR) with high cell density for rapid syngas biomethanation as well as a co-substrate of syngas and organic substances was examined in a long-term fermentation process of 154 days and compared with the reactors of the free cells (FCBR). The RMBR reached maximum capacities of H 2 , CO, and CO 2 conversion of 7.0, 15.2, and 4.0 mmol/L reactor .day, respectively, at the organic loading rate of 3.40 gCOD/L.day. The highest methane production rate from the RMBR was 186.0 mL/L reactor .day on the 147th day, compared to the highest rate in the FCBR, 106.3 mL/L reactor .day, on the 58th day. The RMBR had the ability to maintain a high methanation capacity by retaining the microbial cells, which were at a high risk for cell wash out. Consequently, the system was able to convert more syngas simultaneously with the organic compounds into methane compared to the FCBR.

Research paper thumbnail of Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms

Bioresource Technology, 2015

Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-metha... more Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms, Bioresource Technology (2014), doi: http:// dx.

Research paper thumbnail of Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms

Bioresource Technology, 2015

Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-metha... more Please cite this article as: Youngsukkasem, S., Chandolias, K., Taherzadeh, M.J., Rapid bio-methanation of syngas in a reverse membrane bioreactor: Membrane encased microorganisms, Bioresource Technology (2014), doi: http:// dx.