EVIE KHOO - Academia.edu (original) (raw)
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Colibacillosis is a disease caused by avian pathogenic E. coli (APEC) and is one of the principle... more Colibacillosis is a disease caused by avian pathogenic E. coli (APEC) and is one of the principle cause of morbidity and mortality in poultry worldwide which is represented by a complex syndrome characterized by multiple organ lesions. This study was carried out to determine phylogenetic grouping and virulence associated genes contained by E. coli isolates which is related in causing disease in chicken. E. coli isolates obtained from clinical cases of Veterinary Research Institute were re-identified by conventional methods. Phylogenetic grouping of the isolates was determined by triplex polymerase chain reaction (PCR), and the presence of eight virulence genes were identified by multiplex PCR. A total of 125 E. coli isolates were subjected to analysis of phylogenetic background and virulence associated genes profiling. Phylogenetic analysis demonstrated that most of the E. coli isolated from chicken in this study belonged to group B1 (36.0%), group D (28.0%), group A (27.2%) and gro...
Colibacillosis is an important disease affecting the poultry industry in many countries, caused b... more Colibacillosis is an important disease affecting the poultry industry in many countries, caused by the Avian Pathogenic E. coli (APEC): it manifests as various clinical signs. It contributes significantly to economic loss for poultry farmers as a result of high mortality and morbidity in poultry. To overcome this, antibiotics have been widely used to eliminate E. coli infection in poultry farms in recent years. Treatment with antibiotics has been considered as a vital regimen to control E. coli infection at the farm level for many years. However, high frequency of antibiotic resistance of E. coli isolates from chicken has become the centre of attention due to public health importance. The aim of the present study is to determine the multidrug resistant profiles of E. coli strains isolated from chicken. E. coli isolates obtained from clinical cases were re-identified and classified by conventional methods. Multidrug resistant profiles against 13 different antibiotics of 125 E. coli i...
Genome Announcements, 2017
Human brucellosis is a neglected zoonotic disease and has widespread geographical distribution. B... more Human brucellosis is a neglected zoonotic disease and has widespread geographical distribution. Brucella melitensis has caused outbreaks and sporadic cases in Malaysia. Here, we present the whole-genome sequences of four B. melitensis strains isolated from brucellosis patients in Malaysia.
Colibacillosis is a disease caused by avian pathogenic E. coli (APEC) and is one of the principle... more Colibacillosis is a disease caused by avian pathogenic E. coli (APEC) and is one of the principle cause of morbidity and mortality in poultry worldwide which is represented by a complex syndrome characterized by multiple organ lesions. This study was carried out to determine phylogenetic grouping and virulence associated genes contained by E. coli isolates which is related in causing disease in chicken. E. coli isolates obtained from clinical cases of Veterinary Research Institute were re-identified by conventional methods. Phylogenetic grouping of the isolates was determined by triplex polymerase chain reaction (PCR), and the presence of eight virulence genes were identified by multiplex PCR. A total of 125 E. coli isolates were subjected to analysis of phylogenetic background and virulence associated genes profiling. Phylogenetic analysis demonstrated that most of the E. coli isolated from chicken in this study belonged to group B1 (36.0%), group D (28.0%), group A (27.2%) and gro...
Colibacillosis is an important disease affecting the poultry industry in many countries, caused b... more Colibacillosis is an important disease affecting the poultry industry in many countries, caused by the Avian Pathogenic E. coli (APEC): it manifests as various clinical signs. It contributes significantly to economic loss for poultry farmers as a result of high mortality and morbidity in poultry. To overcome this, antibiotics have been widely used to eliminate E. coli infection in poultry farms in recent years. Treatment with antibiotics has been considered as a vital regimen to control E. coli infection at the farm level for many years. However, high frequency of antibiotic resistance of E. coli isolates from chicken has become the centre of attention due to public health importance. The aim of the present study is to determine the multidrug resistant profiles of E. coli strains isolated from chicken. E. coli isolates obtained from clinical cases were re-identified and classified by conventional methods. Multidrug resistant profiles against 13 different antibiotics of 125 E. coli i...
Genome Announcements, 2017
Human brucellosis is a neglected zoonotic disease and has widespread geographical distribution. B... more Human brucellosis is a neglected zoonotic disease and has widespread geographical distribution. Brucella melitensis has caused outbreaks and sporadic cases in Malaysia. Here, we present the whole-genome sequences of four B. melitensis strains isolated from brucellosis patients in Malaysia.