K. Sterzik - Academia.edu (original) (raw)
Papers by K. Sterzik
International journal of fertility
Human menopausal gonadotropin (hMG) was administered to 10 patients with polycystic ovarian disea... more Human menopausal gonadotropin (hMG) was administered to 10 patients with polycystic ovarian disease (PCOD) who had failed to ovulate in response to clomiphene citrate. Five patients (group 1) were treated with intramuscular hMG injections daily, on an individually adjusted regimen. Five others (group 2) were stimulated with subcutaneous hMG in a pulsatile fashion by means of a portable infusion minipump. The pulse doses ranged between 3.5 and 7.7 IU FSH per pulse at a constant frequency of 90 minutes. Sixteen of 18 treatment cycles were ovulatory, 9 under intramuscular, and 7 under subcutaneous treatment. A total of two patients conceived with singleton pregnancies, one in each treatment group. Neither ovarian hyperstimulation nor complications of injections were noted. The amount of subcutaneous hMG required to achieve ovulation was significantly less (46.5%; P less than .001) than that needed with intramuscular administration. However, there were no differences in the duration of ...
Fertility and Sterility, 1991
Genetic factors, especially numerical chromosome anomalies, play an important role in embryonic l... more Genetic factors, especially numerical chromosome anomalies, play an important role in embryonic loss. Because somatic cell analysis cannot assess the risk of errors arising de novo during germ cell maturation, we investigated whether the male gametes from couples with habitual abortion carry a higher rate of anomalies than those from donors without reproductive dysfunction. Our results indicate that there is no significant difference between the two groups for the total rates of aneuploidy and structural anomalies. However, the levels of chromosome breaks and acentric fragments were significantly higher in the abortion group. The implications of this fact remain to be investigated.
Human Reproduction, 1999
Conclusion: These data indicate that second ejaculates contain greater percentages of motile sper... more Conclusion: These data indicate that second ejaculates contain greater percentages of motile spermatozoa in comparison to first ejaculates in oligoasthenospermic patients. The use of spermatozoa from second ejaculates in ICSI programmes could be suggested. measured in seminal fluid compared to serum concentrations may indicate an important role of this cytokine in spermatogenesis. The observation of a significant difference between concentrations found in fertile versus subfertile men strengthens the hypothesis of a significant role of IL-7 in sperm homeostasis. This is the first report of a possible involvement of IL-7 in spermatogenesis. If this role is related to growth-factor properties, mobilization of sperm cells or apoptosis modulation should be investigated in further studies.
Fertility and Sterility, 2005
To evaluate the ultramorphologic sperm features of idiopathic infertile men after acupuncture the... more To evaluate the ultramorphologic sperm features of idiopathic infertile men after acupuncture therapy. Design: Prospective controlled study.
Archives of Gynecology and Obstetrics, 1993
ABSTRACT
International Journal of Gynecology & Obstetrics, 1989
ABSTRACT
Fertility and Sterility, 2000
Fertility and Sterility, 2001
This study compared rates of pregnancy and in vitro fertilization (IVF) parameters in subjects st... more This study compared rates of pregnancy and in vitro fertilization (IVF) parameters in subjects stimulated with follicle stimulating hormone (FSH) plus either recombinant human luteinizing hormone (r-LH) or human menopausal gonadotropin (hMG) in a long gonadotropin releasing hormone (GnRH) agonist IVF protocol. This cohort study enrolled patients who underwent IVF stimulation with a long GnRH agonist protocol and received FSH plus r-LH or hMG. Outcomes measured included: FSH and LH doses, number of oocytes and embryos obtained, pregnancy rate per cycle, and clinical pregnancy rate per cycle. Stepwise logistic regression was performed on continuous and categorical variables to control for confounding effects between all variables analyzed. There were 122 patients who underwent 122 IVF cycles with long GnRH agonist protocols. Similar baseline parameters existed between groups. Patients that received r-LH per cycle start were higher for patients in the r-LH group (49%) compared to the hMG group ovarian stimulation in long GnRH agonist protocols in good responders. Prospective stud
Journal of submicroscopic cytology and pathology, 1997
This paper belongs to a series of application of the Baccetti's et al. (1995) formula to the ... more This paper belongs to a series of application of the Baccetti's et al. (1995) formula to the submicroscopical mathematical examination of the human spermatozoa used for assisted reproduction. The present experiment concerns partial zona dissection, a technique requiring a careful evaluation of sperm quality in order to predict the success of the program. Our results demonstrate that the sperm submicroscopic characters introduced in the formula are clearly correlated with the result of PZD. In fact the two numbers concerning the sperm ultrastructural quality (percentage and total number of spermatozoa free from defects in the ejaculate) obtained in successful and unsuccessful PZD groups, showed a large difference (P < 0.01). The most important characteristics seem to be the quality of the acrosomal complex, the condition of the chromatin and the mitochondrial assembly. All these characteristics are expressed with largely different means in successful and unsuccessful ejaculate...
The effect of in-vitro treatment by polyvinylpyrrolidone (PVP) on the ultrastructure of human spe... more The effect of in-vitro treatment by polyvinylpyrrolidone (PVP) on the ultrastructure of human spermatozoa has been tested previously with the statistical analysis of B.Baccetti et al. (1995, J. Androl., 16, 356-371). PVP had a primary detrimental action on the plasma membrane, as well as on acrosomal and mitochondrial membranes. Furthermore, membrane damage induces deterioration of the chromatin, axonemal tubules, fibrous sheath, and accessory fibres.
The frequency of diastolic notching in uteroplacental arteries was examined in a collective of 51... more The frequency of diastolic notching in uteroplacental arteries was examined in a collective of 510 patients with uneventful pregnancies and deliveries. Notching appeared to be more frequent in the uterine than arcuate artery. It was seen to increase as pregnancy progressed and was markedly higher in the third compared with the second trimester. Uteroplacental notching occurred more frequently unilaterally and mainly affected contralateral sites of the placenta. Bilateral and retroplacental notching was the exception in normal pregnancies.
Journal of Andrology, 2012
Zentralblatt fur Gynakologie, 1993
Sperm chromosomes from 15 fertile men were analyzed after fusion of their spermatozoa with zona-f... more Sperm chromosomes from 15 fertile men were analyzed after fusion of their spermatozoa with zona-free hamster eggs. The total proportion of abnormal metaphases as well as the proportions of aneuploidy and structural aberrations were calculated for every man and examined for linear correlations with sperm morphology and the age of the persons studied. A positive correlation between the cytogenetic parameters and the percentage of abnormal sperm morphology was not evident, suggesting that assessment of sperm morphology cannot be used as an indicator of chromosomal damage in human spermatozoa. In contrast, there was a more distinct positive correlation between the age of donors and the three cytogenetic parameters studied. Analogous calculations for two other study groups (group S: male subfertility; group HA: habitual abortions of the wives with unknown etiology) provided less clear results that may be attributed to the lower number of patients or to the existence of special indication...
Zentralblatt fur Gynakologie, 1989
Simultaneously with in vitro fertilization (IVF), the acridine orange (AO) test was performed on ... more Simultaneously with in vitro fertilization (IVF), the acridine orange (AO) test was performed on 51 patients according to the method of Tejada et al. This test allows a differentiation of spermatozoa with intact (double-stranded) and denatured (single-sanded) DNA. A significant relationship was demonstrated between the percentage of green-fluorescing (intact) sperm and the results of IVF. For both, the groups with (n = 40) and without (n = 11) fertilization of human eggs, a positive correlation was found between AO-test and sperm motility, and AO-test and normal sperm morphology.
We report on the first pregnancies achieved at our clinic following direct oocyte-sperm-transfer ... more We report on the first pregnancies achieved at our clinic following direct oocyte-sperm-transfer (DOST). Immediately after follicular aspiration, oocytes together with spermatozoa prepared by the swim-up-technique are transferred to the uterus in a manner similar to the embryo transfer following IVF. Our study population consists of 9 patients with bilateral tubal occlusion, accompanied by a male factor in three cases. Up to now, we have achieved four pregnancies, including one twin pregnancy, all occurring, when a male factor was absent. The method described appears promising in terms of efficiency, reduction in the costs and technical procedures during treatment of human infertility.
Of 2232 women with no cytologic evidence of intraepithelial neoplasia, 250 (11.2%) were positive ... more Of 2232 women with no cytologic evidence of intraepithelial neoplasia, 250 (11.2%) were positive for human papillomavirus deoxyribonucleic acid (DNA) by filter in situ hybridization. In 150 of those human papillomaviruspositive patients, an adequate colposcopic examination of the cervix was possible; human papillomavirus infection was diagnosed in 104 women (70%). Cervical cytology showed evidence of human papillomavirus infection in only 23 patients (15%). The following colposcopic features were most common: acetowhite epithelium (29%), punctuation (18%), acetowhite spikes (17%), and mosaicism (9%). Colposcopy was essentially normal in 27%. In 64 hysterectomized patients, vaginal colposcopy showed evidence of human papillomavirus infection in 38 women (59%). Vaginal cytology showed signs of human papillomavirus infection in only 9% (N=6). Acetowhite spikes were seen in 52%, acetowhite epithelium in 5%, punctuation in 3%, and normal findings in 40%. Histologic examination of 25 biop...
Molecular and Cellular Aspects of Periimplantation Processes, 1995
The implantation window is a widely used and remarkably well understood term in reproductive biol... more The implantation window is a widely used and remarkably well understood term in reproductive biology and medicine. It was initiated by Psychoyos (1) and expanded by McLaren (2) and Finn (3) to define whether estrogens in mice or rats control the physiological status of a receptive or nonreceptive endometrium to achieve implantation of the blastocyst. However, the term has mainly been applied only to the concept of an endometrial window. Biologically, this term should be extended to cover all of the components associated with implantation: the blastocyst, the endometrium, and the corpus luteum (CL). Consequently, we should consider an embryonic window, as well as an endometrial and a luteal window. Each of these major events of the implantation window requires description and definition for scientific understanding (4).
Geburtshilfe Und Frauenheilkunde - GEBURTSH FRAUENHEILK, 1991
Gynäkologie und Geburtshilfe 1990, 1991
International journal of fertility
Human menopausal gonadotropin (hMG) was administered to 10 patients with polycystic ovarian disea... more Human menopausal gonadotropin (hMG) was administered to 10 patients with polycystic ovarian disease (PCOD) who had failed to ovulate in response to clomiphene citrate. Five patients (group 1) were treated with intramuscular hMG injections daily, on an individually adjusted regimen. Five others (group 2) were stimulated with subcutaneous hMG in a pulsatile fashion by means of a portable infusion minipump. The pulse doses ranged between 3.5 and 7.7 IU FSH per pulse at a constant frequency of 90 minutes. Sixteen of 18 treatment cycles were ovulatory, 9 under intramuscular, and 7 under subcutaneous treatment. A total of two patients conceived with singleton pregnancies, one in each treatment group. Neither ovarian hyperstimulation nor complications of injections were noted. The amount of subcutaneous hMG required to achieve ovulation was significantly less (46.5%; P less than .001) than that needed with intramuscular administration. However, there were no differences in the duration of ...
Fertility and Sterility, 1991
Genetic factors, especially numerical chromosome anomalies, play an important role in embryonic l... more Genetic factors, especially numerical chromosome anomalies, play an important role in embryonic loss. Because somatic cell analysis cannot assess the risk of errors arising de novo during germ cell maturation, we investigated whether the male gametes from couples with habitual abortion carry a higher rate of anomalies than those from donors without reproductive dysfunction. Our results indicate that there is no significant difference between the two groups for the total rates of aneuploidy and structural anomalies. However, the levels of chromosome breaks and acentric fragments were significantly higher in the abortion group. The implications of this fact remain to be investigated.
Human Reproduction, 1999
Conclusion: These data indicate that second ejaculates contain greater percentages of motile sper... more Conclusion: These data indicate that second ejaculates contain greater percentages of motile spermatozoa in comparison to first ejaculates in oligoasthenospermic patients. The use of spermatozoa from second ejaculates in ICSI programmes could be suggested. measured in seminal fluid compared to serum concentrations may indicate an important role of this cytokine in spermatogenesis. The observation of a significant difference between concentrations found in fertile versus subfertile men strengthens the hypothesis of a significant role of IL-7 in sperm homeostasis. This is the first report of a possible involvement of IL-7 in spermatogenesis. If this role is related to growth-factor properties, mobilization of sperm cells or apoptosis modulation should be investigated in further studies.
Fertility and Sterility, 2005
To evaluate the ultramorphologic sperm features of idiopathic infertile men after acupuncture the... more To evaluate the ultramorphologic sperm features of idiopathic infertile men after acupuncture therapy. Design: Prospective controlled study.
Archives of Gynecology and Obstetrics, 1993
ABSTRACT
International Journal of Gynecology & Obstetrics, 1989
ABSTRACT
Fertility and Sterility, 2000
Fertility and Sterility, 2001
This study compared rates of pregnancy and in vitro fertilization (IVF) parameters in subjects st... more This study compared rates of pregnancy and in vitro fertilization (IVF) parameters in subjects stimulated with follicle stimulating hormone (FSH) plus either recombinant human luteinizing hormone (r-LH) or human menopausal gonadotropin (hMG) in a long gonadotropin releasing hormone (GnRH) agonist IVF protocol. This cohort study enrolled patients who underwent IVF stimulation with a long GnRH agonist protocol and received FSH plus r-LH or hMG. Outcomes measured included: FSH and LH doses, number of oocytes and embryos obtained, pregnancy rate per cycle, and clinical pregnancy rate per cycle. Stepwise logistic regression was performed on continuous and categorical variables to control for confounding effects between all variables analyzed. There were 122 patients who underwent 122 IVF cycles with long GnRH agonist protocols. Similar baseline parameters existed between groups. Patients that received r-LH per cycle start were higher for patients in the r-LH group (49%) compared to the hMG group ovarian stimulation in long GnRH agonist protocols in good responders. Prospective stud
Journal of submicroscopic cytology and pathology, 1997
This paper belongs to a series of application of the Baccetti's et al. (1995) formula to the ... more This paper belongs to a series of application of the Baccetti's et al. (1995) formula to the submicroscopical mathematical examination of the human spermatozoa used for assisted reproduction. The present experiment concerns partial zona dissection, a technique requiring a careful evaluation of sperm quality in order to predict the success of the program. Our results demonstrate that the sperm submicroscopic characters introduced in the formula are clearly correlated with the result of PZD. In fact the two numbers concerning the sperm ultrastructural quality (percentage and total number of spermatozoa free from defects in the ejaculate) obtained in successful and unsuccessful PZD groups, showed a large difference (P < 0.01). The most important characteristics seem to be the quality of the acrosomal complex, the condition of the chromatin and the mitochondrial assembly. All these characteristics are expressed with largely different means in successful and unsuccessful ejaculate...
The effect of in-vitro treatment by polyvinylpyrrolidone (PVP) on the ultrastructure of human spe... more The effect of in-vitro treatment by polyvinylpyrrolidone (PVP) on the ultrastructure of human spermatozoa has been tested previously with the statistical analysis of B.Baccetti et al. (1995, J. Androl., 16, 356-371). PVP had a primary detrimental action on the plasma membrane, as well as on acrosomal and mitochondrial membranes. Furthermore, membrane damage induces deterioration of the chromatin, axonemal tubules, fibrous sheath, and accessory fibres.
The frequency of diastolic notching in uteroplacental arteries was examined in a collective of 51... more The frequency of diastolic notching in uteroplacental arteries was examined in a collective of 510 patients with uneventful pregnancies and deliveries. Notching appeared to be more frequent in the uterine than arcuate artery. It was seen to increase as pregnancy progressed and was markedly higher in the third compared with the second trimester. Uteroplacental notching occurred more frequently unilaterally and mainly affected contralateral sites of the placenta. Bilateral and retroplacental notching was the exception in normal pregnancies.
Journal of Andrology, 2012
Zentralblatt fur Gynakologie, 1993
Sperm chromosomes from 15 fertile men were analyzed after fusion of their spermatozoa with zona-f... more Sperm chromosomes from 15 fertile men were analyzed after fusion of their spermatozoa with zona-free hamster eggs. The total proportion of abnormal metaphases as well as the proportions of aneuploidy and structural aberrations were calculated for every man and examined for linear correlations with sperm morphology and the age of the persons studied. A positive correlation between the cytogenetic parameters and the percentage of abnormal sperm morphology was not evident, suggesting that assessment of sperm morphology cannot be used as an indicator of chromosomal damage in human spermatozoa. In contrast, there was a more distinct positive correlation between the age of donors and the three cytogenetic parameters studied. Analogous calculations for two other study groups (group S: male subfertility; group HA: habitual abortions of the wives with unknown etiology) provided less clear results that may be attributed to the lower number of patients or to the existence of special indication...
Zentralblatt fur Gynakologie, 1989
Simultaneously with in vitro fertilization (IVF), the acridine orange (AO) test was performed on ... more Simultaneously with in vitro fertilization (IVF), the acridine orange (AO) test was performed on 51 patients according to the method of Tejada et al. This test allows a differentiation of spermatozoa with intact (double-stranded) and denatured (single-sanded) DNA. A significant relationship was demonstrated between the percentage of green-fluorescing (intact) sperm and the results of IVF. For both, the groups with (n = 40) and without (n = 11) fertilization of human eggs, a positive correlation was found between AO-test and sperm motility, and AO-test and normal sperm morphology.
We report on the first pregnancies achieved at our clinic following direct oocyte-sperm-transfer ... more We report on the first pregnancies achieved at our clinic following direct oocyte-sperm-transfer (DOST). Immediately after follicular aspiration, oocytes together with spermatozoa prepared by the swim-up-technique are transferred to the uterus in a manner similar to the embryo transfer following IVF. Our study population consists of 9 patients with bilateral tubal occlusion, accompanied by a male factor in three cases. Up to now, we have achieved four pregnancies, including one twin pregnancy, all occurring, when a male factor was absent. The method described appears promising in terms of efficiency, reduction in the costs and technical procedures during treatment of human infertility.
Of 2232 women with no cytologic evidence of intraepithelial neoplasia, 250 (11.2%) were positive ... more Of 2232 women with no cytologic evidence of intraepithelial neoplasia, 250 (11.2%) were positive for human papillomavirus deoxyribonucleic acid (DNA) by filter in situ hybridization. In 150 of those human papillomaviruspositive patients, an adequate colposcopic examination of the cervix was possible; human papillomavirus infection was diagnosed in 104 women (70%). Cervical cytology showed evidence of human papillomavirus infection in only 23 patients (15%). The following colposcopic features were most common: acetowhite epithelium (29%), punctuation (18%), acetowhite spikes (17%), and mosaicism (9%). Colposcopy was essentially normal in 27%. In 64 hysterectomized patients, vaginal colposcopy showed evidence of human papillomavirus infection in 38 women (59%). Vaginal cytology showed signs of human papillomavirus infection in only 9% (N=6). Acetowhite spikes were seen in 52%, acetowhite epithelium in 5%, punctuation in 3%, and normal findings in 40%. Histologic examination of 25 biop...
Molecular and Cellular Aspects of Periimplantation Processes, 1995
The implantation window is a widely used and remarkably well understood term in reproductive biol... more The implantation window is a widely used and remarkably well understood term in reproductive biology and medicine. It was initiated by Psychoyos (1) and expanded by McLaren (2) and Finn (3) to define whether estrogens in mice or rats control the physiological status of a receptive or nonreceptive endometrium to achieve implantation of the blastocyst. However, the term has mainly been applied only to the concept of an endometrial window. Biologically, this term should be extended to cover all of the components associated with implantation: the blastocyst, the endometrium, and the corpus luteum (CL). Consequently, we should consider an embryonic window, as well as an endometrial and a luteal window. Each of these major events of the implantation window requires description and definition for scientific understanding (4).
Geburtshilfe Und Frauenheilkunde - GEBURTSH FRAUENHEILK, 1991
Gynäkologie und Geburtshilfe 1990, 1991