K. Targońska - Academia.edu (original) (raw)

Papers by K. Targońska

This review presents the results of papers published from 1990 to 2008 on the reproductive biotec... more This review presents the results of papers published from 1990 to 2008 on the reproductive biotechnology of rheophilic cyprinid fish of the genus Leuciscus: ide, Leuciscus idus (L.), dace, Leuciscus leuciscus (L.), and chub, Leuciscus cephalus (L.). The issues studied included obtaining reproductors both from the wild and through aquaculture. Particular attention was paid to issues linked with the insufficient quality of spawners, which stemmed most frequently from their inappropriate handling. The effects of stimulating rheophilic cyprinid fish with various hormonal preparations was analyzed. Initially, carp pituitary homogenate (CPH) was used, and for a long period this was the only preparation in use. Later, human chorionic gonadotropin (hCG) was used alone or in combination with CPH. It was confirmed that hCG is not suitable for the reproduction of rheophilic cyprinid fish. In the late 1990s, a gonadotropin releasing hormone analogue (GnRH) was introduced. Initially, Ovopel was used, which is a mammalian analogue of GnRH (D-Ala 6 Pro 9 Net-mGnRH) with a dopamine receptor antagonist (metoclopramide). In recent years, studies have begun on the use of Ovaprim, a salmon analogue of GnRH (D-Arg 6 Pro 9 Net-sGnRH) with a dopamine receptor antagonist (domperidone), and on GnRHa without a dopamine receptor antagonist. It has been confirmed that hormonal stimulation combined with stimulation through the manipulation of environmental conditions is essential for the reproduction of rheophilic cyprinid fish species of the genus Leuciscus under controlled conditions. The effects of reproduction under controlled conditions differ significantly among the species analyzed. The lowest percentage of ovulating females and spermiating males was noted in chub. Genome manipulation performed on the studied species is also described, including gynogenesis and androgenesis. The latter, in combination with semen cryopreservation, creates possibilities for restoring threatened populations and species.

Burbot (Lota lota L.) is one of the most endangered fish species in Poland and in mny others Euro... more Burbot (Lota lota L.) is one of the most endangered fish species in Poland and in mny others European countries. Additionally, potentially might be a new species for freshwater coldwater aquaculture. For this reason it is necessary to develop methods of artificial spawning and rearing larvae under controlled conditions. The data on spawning of burbot in captivity are very limited. The aim of this study was investigate the artificial reproduction of burbot in captivity. Spawners survival was very high (over 97%) when fish were kept even for for four months under the hatchery conditions. Only about 50% of burbot spawners are trout pellets, whereas more than 70% individuals fed forage fish (frozen). Oogenesis in all fish, which took food, proceeded properly what resulted in ovulation after hormonal stimulation. Less than 30% of spawners were not mature; it might be connected with spawning behaviour of this species what was reported earlier that in natural burbot populations some part (...

The aim of the study was to conduct off-season spawning of perch, Perca fluviatilis L. Spawners w... more The aim of the study was to conduct off-season spawning of perch, Perca fluviatilis L. Spawners were obtained in late September from Lake Kortowskie. The fish were transported to the hatchery and divided into two size groups: G1 -small fish (30-70 g), and G2 -large fish (200-400 g). All males from G1 group spermiated after a two-month period of photo-thermal stimulation, but only 25% of the males from G2 group produced milt. Better results were obtained with fish that were stimulated after three months of chilling. All the stimulated males spermiated and produced higher volumes of milt. The best results, defined as ovulation success and embryo survival to the eyed-egg-stage, as well as spawner survival rate, were obtained with large females (G2-B) stimulated with a double dose of hormone (2 Ovopel pellets kg -1 ). The egg strands obtained from females from group A (small and large fish stimulated with GnRHa and metoclopramide at 0.1 and 1 pellet kg -1 after 24 hr) were usually fragmented. All females responded to the hormonal injections after 4 to 5 days.