Kaishi Satomi - Academia.edu (original) (raw)

Papers by Kaishi Satomi

Research paper thumbnail of Epileptogenic glioma in a 4-year-old child: a case report

Brain Tumor Pathology, 2010

We report a rare case of epileptogenic glioma composed of glial progenitor cells that differentia... more We report a rare case of epileptogenic glioma composed of glial progenitor cells that differentiated into an astrocytic and oligodendrocytic tumor. This 4-year-old girl presented with a 1-year history of complex partial seizure. MR scan showed a mass in the left temporal lobe with a cyst and a contrast-enhanced component. Subtotal resection of the tumor was achieved. Histological examination revealed that the tumor exhibited low cellularity composed of astrocytic and oligodendrocytic components, as well as low mitotic activity (MIB-1 = 1%). Immunohistochemical examination revealed GFAP positivity within the astrocytic cells, olig2 positivity within the oligodendrocytic cells, and S100 positivity in both cell types. MAP2 and CD34 were negative, and neurofilament was only positive in preexisting neurons. The pathological diagnosis was epileptogenic glioma (grade I) composed of glial progenitor cells. The postoperative course has been uneventful with good seizure control for 3 years.

Research paper thumbnail of Increased cytoplasmic S100A6 expression is associated with pulmonary adenocarcinoma progression

Pathology International, 2009

S100A6 is a calcium-binding protein implicated in many cellular processes and frequently upregula... more S100A6 is a calcium-binding protein implicated in many cellular processes and frequently upregulated in cancer. Recently it was reported that S100A6 is one of the genes having higher expression in adenocarcinoma mixed subtype with a bronchioloalveolar carcinoma (BAC) component than in pure BAC. To clarify the association of S100A6 expression with stepwise progression of lung adenocarcinoma, S100A6 protein expression was examined on immunohistochemistry in 92 formalin-fixed and paraffin-embedded lung adenocarcinomas. Both the nucleus and cytoplasm of the tumor cells were stained, and the nuclear and cytoplasmic expression of S100A6 was assessed individually. In addition, six frozen surgical specimens were selected, and the expression of S100A6 was confirmed on western blotting. As a result, although it was not possible to detect any significant correlation between nuclear S100A6 immunoreactivity and tumor progression, advanced adenocarcinoma had significantly higher cytoplasmic S100A6 expression than non-invasive lesions or normal lung tissue (P < 0.05). Moreover, the BAC component tended to have weaker staining than any of the other components. These findings indicate that S100A6 may be associated with the stepwise progression and/or invasion of lung adenocarcinoma, especially BAC-type adenocarcinoma. The present results suggest the utility of S100A6 immunohistochemistry as a marker for estimation of malignancy in adenocarcinoma with a BAC component.

Research paper thumbnail of Bronchioloalveolar Carcinoma (Lepidic Growth) Component Is a More Useful Prognostic Factor than Lymph Node Metastasis

Journal of Thoracic Oncology, 2009

Although many factors predictive of patient survival have been reported for lung cancer, no compa... more Although many factors predictive of patient survival have been reported for lung cancer, no comparative studies have attempted to determine those that are most significant for practical medicine. We conducted a retrospective review of 139 patients who underwent complete resection of adenocarcinomas less than 2 cm in diameter between 1993 and 2000 at the National Cancer Center Hospital (Tokyo, Japan). The MIB-1 labeling index (LI), immunohistochemical staining for carcinoembryonic antigen (CEA), p53, p27, epidermal growth factor receptor (EGFR), phosphorylated-EGFR (pEGFR), Cox-2, neuronatin, gammaH2AX, and thyroid transcription factor-1 (TTF-1), the prevalence of a micropapillary pattern, and the ratio of the bronchioloalveolar cell carcinoma (BAC) or lepidic growth (LG) component were determined, and their significance as prognostic factors for lung adenocarcinoma was compared. Univariate analysis showed that lymph node metastasis (p-N status), BAC/LG component, vascular invasion (p-V status), MIB-1 LI, pEGFR, and CEA were prognostically significant (p-N status: p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001, BAC/LG: p = 0.0005, p-V status: 0.002, MIB-1 LI: p = 0.005, pEGFR: p = 0.024, and CEA: p = 0.049). Multivariate analysis showed that only p-N status (p = 0.013) was of prognostic significance. However, BAC/LG component (p = 0.051) was a more reliable prognostic factor than p-N status in mixed adenocarcinoma with a BAC/LG component. In comparison with other immunohistochemical and histopathologic factors, BAC/LG component is independently and reliably prognostic for small adenocarcinoma of the lung, and, in particular, for the major histologic subtype (adenocarcinoma mixed subtype with BAC/LG), BAC/LG component is more reliably prognostic than lymph node metastasis.

Research paper thumbnail of Nuclear grading of primary pulmonary adenocarcinomas: Correlation between nuclear size and prognosis

Cancer, 2010

BACKGROUND:According to the World Health Organization Classification of Tumors, the prognostic va... more BACKGROUND:According to the World Health Organization Classification of Tumors, the prognostic value of morphometric cytologic atypia has not been assessed in pulmonary adenocarcinoma.According to the World Health Organization Classification of Tumors, the prognostic value of morphometric cytologic atypia has not been assessed in pulmonary adenocarcinoma.METHODS:Primary tumors of 133 pulmonary adenocarcinomas ≤2 cm were analyzed using an image processor for analytical pathology. The results were evaluated using receiver operator characteristic curve analysis, and survival curves were drawn by the Kaplan-Meier method. Furthermore, the results were applied to routine histological diagnosis. Four pathologists evaluated the nuclear factors relative to the size of small lymphocytes as a standard.Primary tumors of 133 pulmonary adenocarcinomas ≤2 cm were analyzed using an image processor for analytical pathology. The results were evaluated using receiver operator characteristic curve analysis, and survival curves were drawn by the Kaplan-Meier method. Furthermore, the results were applied to routine histological diagnosis. Four pathologists evaluated the nuclear factors relative to the size of small lymphocytes as a standard.RESULTS:By using the nuclear area and nuclear major axis dimension, lung adenocarcinomas were divisible into 2 groups showing extremely favorable prognosis and fairly favorable prognosis, without considering histological features or classification. A nuclear area level of <67 μm2 was correlated with longer survival (P < .0001), and the 5-year survival rate was 90.4%. Similarly, a nuclear diameter level of <0.7 μm was correlated with longer survival (P = .0002), and the 5-year survival rate was 88.6%. The mean (±standard deviation [SD]) value of the kappa statistic for the 4 pathologists who evaluated the cases using the size of small lymphocytes as a standard was 0.58 ± 0.10, and the mean (±SD) value of the accuracy metric was 0.66 ± 0.10.By using the nuclear area and nuclear major axis dimension, lung adenocarcinomas were divisible into 2 groups showing extremely favorable prognosis and fairly favorable prognosis, without considering histological features or classification. A nuclear area level of <67 μm2 was correlated with longer survival (P < .0001), and the 5-year survival rate was 90.4%. Similarly, a nuclear diameter level of <0.7 μm was correlated with longer survival (P = .0002), and the 5-year survival rate was 88.6%. The mean (±standard deviation [SD]) value of the kappa statistic for the 4 pathologists who evaluated the cases using the size of small lymphocytes as a standard was 0.58 ± 0.10, and the mean (±SD) value of the accuracy metric was 0.66 ± 0.10.CONCLUSIONS:Nuclear area and nuclear major dimension are 2 useful independent markers for evaluating the prognosis of lung adenocarcinoma. Cancer 2010. © 2010 American Cancer Society.Nuclear area and nuclear major dimension are 2 useful independent markers for evaluating the prognosis of lung adenocarcinoma. Cancer 2010. © 2010 American Cancer Society.

Research paper thumbnail of Specific expression of ZO-1 and N -cadherin in rosette structures of various tumors: possible recapitulation of neural tube formation in embryogenesis and utility as a potentially novel immunohistochemical marker of rosette formation in pulmonary neuroendocrine tumors

Virchows Archiv

Neuroendocrine tumors can develop in various organs. All of these tumors are designated on the ba... more Neuroendocrine tumors can develop in various organs. All of these tumors are designated on the basis of their morphologic characteristics evident by light microscopy, and by immunohistochemistry for antigens such as synaptophysin, chromogranin-A, and CD56/NCAM. In the present study, we attempted to demonstrate the localization of Zonula occludens-1 (ZO-1) and N-cadherin in rosette structures of neuroendocrine tumors using immunohistochemistry and to clarify their specific distribution in rosettes in human pulmonary neuroendocrine tumors in comparison with various types of adenocarcinoma. Among 40 neuroendocrine tumors of the lung examined, 18 cases (45%) and 22 cases (55%) were positive for ZO-1 and N-cadherin, respectively. In addition, we divided the cases into two types: 16 cases of Flexner-type tumor and 24 cases of Homer–Wright-type tumor. We then determined the Rosette Index (RoI; the percentage fraction of rosette structures positive for ZO-1 or N-cadherin among the total number of rosette structures). The Flexner-type neuroendocrine tumors showed significantly higher levels of RoI in ZO-1 than the Homer–Wright-type neuroendocrine tumors (median; 38.8% vs 0%, p N-cadherin and ZO-1 were hardly detected in tubular adenocarcinomas in various organs, and their immunoreactivities differed significantly between adenocarcinoma and pulmonary neuroendocrine tumor (ZO-1, mean 0.23% vs 18%, p N-cadherin, mean 0% vs 33%, p N-cadherin may reflect the mechanisms leading to rosette formation in neuroendocrine tumors, which possibly recapitulate neural tube formation in embryogenesis and could represent a specific immunohistochemical marker for neuroendocrine carcinoma of the lung.

Research paper thumbnail of Epileptogenic glioma in a 4-year-old child: a case report

Brain Tumor Pathology, 2010

We report a rare case of epileptogenic glioma composed of glial progenitor cells that differentia... more We report a rare case of epileptogenic glioma composed of glial progenitor cells that differentiated into an astrocytic and oligodendrocytic tumor. This 4-year-old girl presented with a 1-year history of complex partial seizure. MR scan showed a mass in the left temporal lobe with a cyst and a contrast-enhanced component. Subtotal resection of the tumor was achieved. Histological examination revealed that the tumor exhibited low cellularity composed of astrocytic and oligodendrocytic components, as well as low mitotic activity (MIB-1 = 1%). Immunohistochemical examination revealed GFAP positivity within the astrocytic cells, olig2 positivity within the oligodendrocytic cells, and S100 positivity in both cell types. MAP2 and CD34 were negative, and neurofilament was only positive in preexisting neurons. The pathological diagnosis was epileptogenic glioma (grade I) composed of glial progenitor cells. The postoperative course has been uneventful with good seizure control for 3 years.

Research paper thumbnail of Increased cytoplasmic S100A6 expression is associated with pulmonary adenocarcinoma progression

Pathology International, 2009

S100A6 is a calcium-binding protein implicated in many cellular processes and frequently upregula... more S100A6 is a calcium-binding protein implicated in many cellular processes and frequently upregulated in cancer. Recently it was reported that S100A6 is one of the genes having higher expression in adenocarcinoma mixed subtype with a bronchioloalveolar carcinoma (BAC) component than in pure BAC. To clarify the association of S100A6 expression with stepwise progression of lung adenocarcinoma, S100A6 protein expression was examined on immunohistochemistry in 92 formalin-fixed and paraffin-embedded lung adenocarcinomas. Both the nucleus and cytoplasm of the tumor cells were stained, and the nuclear and cytoplasmic expression of S100A6 was assessed individually. In addition, six frozen surgical specimens were selected, and the expression of S100A6 was confirmed on western blotting. As a result, although it was not possible to detect any significant correlation between nuclear S100A6 immunoreactivity and tumor progression, advanced adenocarcinoma had significantly higher cytoplasmic S100A6 expression than non-invasive lesions or normal lung tissue (P < 0.05). Moreover, the BAC component tended to have weaker staining than any of the other components. These findings indicate that S100A6 may be associated with the stepwise progression and/or invasion of lung adenocarcinoma, especially BAC-type adenocarcinoma. The present results suggest the utility of S100A6 immunohistochemistry as a marker for estimation of malignancy in adenocarcinoma with a BAC component.

Research paper thumbnail of Bronchioloalveolar Carcinoma (Lepidic Growth) Component Is a More Useful Prognostic Factor than Lymph Node Metastasis

Journal of Thoracic Oncology, 2009

Although many factors predictive of patient survival have been reported for lung cancer, no compa... more Although many factors predictive of patient survival have been reported for lung cancer, no comparative studies have attempted to determine those that are most significant for practical medicine. We conducted a retrospective review of 139 patients who underwent complete resection of adenocarcinomas less than 2 cm in diameter between 1993 and 2000 at the National Cancer Center Hospital (Tokyo, Japan). The MIB-1 labeling index (LI), immunohistochemical staining for carcinoembryonic antigen (CEA), p53, p27, epidermal growth factor receptor (EGFR), phosphorylated-EGFR (pEGFR), Cox-2, neuronatin, gammaH2AX, and thyroid transcription factor-1 (TTF-1), the prevalence of a micropapillary pattern, and the ratio of the bronchioloalveolar cell carcinoma (BAC) or lepidic growth (LG) component were determined, and their significance as prognostic factors for lung adenocarcinoma was compared. Univariate analysis showed that lymph node metastasis (p-N status), BAC/LG component, vascular invasion (p-V status), MIB-1 LI, pEGFR, and CEA were prognostically significant (p-N status: p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.0001, BAC/LG: p = 0.0005, p-V status: 0.002, MIB-1 LI: p = 0.005, pEGFR: p = 0.024, and CEA: p = 0.049). Multivariate analysis showed that only p-N status (p = 0.013) was of prognostic significance. However, BAC/LG component (p = 0.051) was a more reliable prognostic factor than p-N status in mixed adenocarcinoma with a BAC/LG component. In comparison with other immunohistochemical and histopathologic factors, BAC/LG component is independently and reliably prognostic for small adenocarcinoma of the lung, and, in particular, for the major histologic subtype (adenocarcinoma mixed subtype with BAC/LG), BAC/LG component is more reliably prognostic than lymph node metastasis.

Research paper thumbnail of Nuclear grading of primary pulmonary adenocarcinomas: Correlation between nuclear size and prognosis

Cancer, 2010

BACKGROUND:According to the World Health Organization Classification of Tumors, the prognostic va... more BACKGROUND:According to the World Health Organization Classification of Tumors, the prognostic value of morphometric cytologic atypia has not been assessed in pulmonary adenocarcinoma.According to the World Health Organization Classification of Tumors, the prognostic value of morphometric cytologic atypia has not been assessed in pulmonary adenocarcinoma.METHODS:Primary tumors of 133 pulmonary adenocarcinomas ≤2 cm were analyzed using an image processor for analytical pathology. The results were evaluated using receiver operator characteristic curve analysis, and survival curves were drawn by the Kaplan-Meier method. Furthermore, the results were applied to routine histological diagnosis. Four pathologists evaluated the nuclear factors relative to the size of small lymphocytes as a standard.Primary tumors of 133 pulmonary adenocarcinomas ≤2 cm were analyzed using an image processor for analytical pathology. The results were evaluated using receiver operator characteristic curve analysis, and survival curves were drawn by the Kaplan-Meier method. Furthermore, the results were applied to routine histological diagnosis. Four pathologists evaluated the nuclear factors relative to the size of small lymphocytes as a standard.RESULTS:By using the nuclear area and nuclear major axis dimension, lung adenocarcinomas were divisible into 2 groups showing extremely favorable prognosis and fairly favorable prognosis, without considering histological features or classification. A nuclear area level of <67 μm2 was correlated with longer survival (P < .0001), and the 5-year survival rate was 90.4%. Similarly, a nuclear diameter level of <0.7 μm was correlated with longer survival (P = .0002), and the 5-year survival rate was 88.6%. The mean (±standard deviation [SD]) value of the kappa statistic for the 4 pathologists who evaluated the cases using the size of small lymphocytes as a standard was 0.58 ± 0.10, and the mean (±SD) value of the accuracy metric was 0.66 ± 0.10.By using the nuclear area and nuclear major axis dimension, lung adenocarcinomas were divisible into 2 groups showing extremely favorable prognosis and fairly favorable prognosis, without considering histological features or classification. A nuclear area level of <67 μm2 was correlated with longer survival (P < .0001), and the 5-year survival rate was 90.4%. Similarly, a nuclear diameter level of <0.7 μm was correlated with longer survival (P = .0002), and the 5-year survival rate was 88.6%. The mean (±standard deviation [SD]) value of the kappa statistic for the 4 pathologists who evaluated the cases using the size of small lymphocytes as a standard was 0.58 ± 0.10, and the mean (±SD) value of the accuracy metric was 0.66 ± 0.10.CONCLUSIONS:Nuclear area and nuclear major dimension are 2 useful independent markers for evaluating the prognosis of lung adenocarcinoma. Cancer 2010. © 2010 American Cancer Society.Nuclear area and nuclear major dimension are 2 useful independent markers for evaluating the prognosis of lung adenocarcinoma. Cancer 2010. © 2010 American Cancer Society.

Research paper thumbnail of Specific expression of ZO-1 and N -cadherin in rosette structures of various tumors: possible recapitulation of neural tube formation in embryogenesis and utility as a potentially novel immunohistochemical marker of rosette formation in pulmonary neuroendocrine tumors

Virchows Archiv

Neuroendocrine tumors can develop in various organs. All of these tumors are designated on the ba... more Neuroendocrine tumors can develop in various organs. All of these tumors are designated on the basis of their morphologic characteristics evident by light microscopy, and by immunohistochemistry for antigens such as synaptophysin, chromogranin-A, and CD56/NCAM. In the present study, we attempted to demonstrate the localization of Zonula occludens-1 (ZO-1) and N-cadherin in rosette structures of neuroendocrine tumors using immunohistochemistry and to clarify their specific distribution in rosettes in human pulmonary neuroendocrine tumors in comparison with various types of adenocarcinoma. Among 40 neuroendocrine tumors of the lung examined, 18 cases (45%) and 22 cases (55%) were positive for ZO-1 and N-cadherin, respectively. In addition, we divided the cases into two types: 16 cases of Flexner-type tumor and 24 cases of Homer–Wright-type tumor. We then determined the Rosette Index (RoI; the percentage fraction of rosette structures positive for ZO-1 or N-cadherin among the total number of rosette structures). The Flexner-type neuroendocrine tumors showed significantly higher levels of RoI in ZO-1 than the Homer–Wright-type neuroendocrine tumors (median; 38.8% vs 0%, p N-cadherin and ZO-1 were hardly detected in tubular adenocarcinomas in various organs, and their immunoreactivities differed significantly between adenocarcinoma and pulmonary neuroendocrine tumor (ZO-1, mean 0.23% vs 18%, p N-cadherin, mean 0% vs 33%, p N-cadherin may reflect the mechanisms leading to rosette formation in neuroendocrine tumors, which possibly recapitulate neural tube formation in embryogenesis and could represent a specific immunohistochemical marker for neuroendocrine carcinoma of the lung.