Jogen Kalita - Academia.edu (original) (raw)

Papers by Jogen Kalita

Life, Nov 25, 2022

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Journal of Applied Pharmaceutical Science

The objective of the study was to validate the antifertility effect of Persicaria hydropiper on t... more The objective of the study was to validate the antifertility effect of Persicaria hydropiper on the reproductive physiology in female albino mice. Female albino mice were orally administered methanolic root extract of P. hydropiper at doses of 300 mg/kg body weight and 600 mg/kg body weight daily for 7 and 15 days and the effect on reproductive organs' weight and histomorphometry was investigated. In the positive control group, 17β-estradiol (E 2) (1 μg/kg body weight) was injected subcutaneously daily for 7 and 15 days. Persicaria hydropiper significantly reduced ovary and uterus weight in a dose-dependent manner. Compared to the control, the number of developing follicles was significantly reduced with significantly increased atretic follicles and the presence of pyknotic granulosa cells in the ovary of P. hydropiper root extract-treated mice. Histomorphometric changes in the uterine tissue of the extract-treated mice include reduction in endometrial thickness, height of luminal epithelium, diameter of the lumen, and number of endometrial glands. The results of the present study reveal that P. hydropiper causes atrophy in the uterine tissue and disrupts folliculogenesis that may be responsible for the reduced number of Graafian follicle and corpus luteum and increased atretic follicles, thereby rendering an antifertility effect.

Molecules

Herbal products have been used in traditional systems of medicine and by ethnic healers for ages ... more Herbal products have been used in traditional systems of medicine and by ethnic healers for ages to treat various diseases. Currently, it is estimated that about 80% of people worldwide use herbal traditional medicines against various ailments, partly due to easy accessibility and low cost, and the lower side effects they pose. Bergenia ligulata, a herb ranging from the Himalayas to the foothills, including the north-eastern states of India, has traditionally been used as a remedy against various diseases, most prominently kidney stones. The medicinal properties of B. ligulata have been attributed to bergenin, its most potent bioactive component. Apart from bergenin, the other compounds available in B. ligulata are arbutin, gallic acid, protocatechuic acid, chlorogenic acid, syringic acid, catechin, ferulic acid, afzelechin, paashaanolactone, caryophyllene, 1,8-cineole, β-eudesmol, stigmasterol, β-sitosterol, parasorbic acid, 3-methyl-2-buten-1-ol, phytol, terpinen-4-ol, tannic acid...

International Journal of Life Science and Pharma Research

Plants have long been valued for their medicinal properties. The secret to efficient treatment is... more Plants have long been valued for their medicinal properties. The secret to efficient treatment is finding the right herb. The purpose of this review is to provide an updated overview of Glycyrrhiza glabra L., “the guardian of herbs” regarding its traditional uses, phytochemistry, and emphasizing the relevance of a few key characteristics of this plant. This review covers a combination of all aspects from an aphrodisiac point of view that were missed by previous articles. This article is presented to include all of the most recent information on its phytochemical and pharmacological effects, which were investigated using a variety of approaches. The plant can be a suitable candidate to manage the long fought erectile dysfunction in men. The extract and the constituents have been found effective in inducing NO signaling pathway that has beneficial effects in initiation and maintenance of erection. It is an age-old plant that belongs to the family Fabaceae. G. glabra (Licorice) has bee...

Research Journal of Biotechnology, 2022

Organophosphates comprise several groups of chemicals derived from phosphonic, phosphinic and pho... more Organophosphates comprise several groups of chemicals derived from phosphonic, phosphinic and phosphoric acids. The chemicals cause four major neurotoxic disorders: cholinergic syndrome, chronic organophosphate-induced neuropsychiatric disorder (COPIND), intermediate syndrome and organophosphate-induced delayed polyneuropathy (OPIDP). In the cholinergic syndrome, organophosphates exert their toxic effect through the inhibition of acetylcholinesterase. Patients with cholinergic syndrome experience several symptoms including sweating, lacrimation, salivation and rhinorrhea and respiratory difficulties. The primary symptoms in the intermediate syndrome are respiratory and proximal limb muscle weakness. In contrast, psychiatric conditions, such as depression and anxiety, have been reported in patients with COPIND. In OPIDP, patients experience acute cramping pain inside their calves and itchiness in the hands and feet, paresthesia and distal unresponsiveness.

Mucuna pruriens is an annual, climbing shrub which has an important place among aphrodisiac herbs... more Mucuna pruriens is an annual, climbing shrub which has an important place among aphrodisiac herbs in India since the ancient times. The plant has been using traditionally for many medicinal purposes such as infertility, Parkinson’s disease, loss of libido, antioxidant, anti venom, anti microbial etc. The present study was carried out to investigate the preliminary phytochemical analysis and evaluation of the effect on the uterus of ovariectomized mice. Methanol extract of the seeds was used for the study and applied to the mice for seven consecutive days. On the eighth day all the animals were sacrificed and the uteri were dissected out to see the effect of the extract on it. Result showed that the weight of the extract treated uteri was increased compared to that of the control mice along with the increase in the total uterine protein. Histological architecture of the uteri of the treated mice also showed changes compared to control.

Genistein and coumestrol are potent dietary phytoestrogens which interact differentially with est... more Genistein and coumestrol are potent dietary phytoestrogens which interact differentially with estrogen receptors and thus mediate comparable estrogenicity. However, data on their combinatory effects in vivo are limited. We assessed individual and combined responses on uterine estrogen-sensitive endpoints and uterine gene expression in ovariectomized mice following subcutaneous treatment for 3 consecutive days, 14 d after ovariectomy. Effects on uterine wet weight, luminal epithelial cell height, stromal gland number and uterine ERα, ERβ and PR mRNA expression were determined using qRT-PCR. 17β-estradiol acted as positive control. Coumestrol singly exhibited stronger uterotrophic responses than genistein. However, combinations significantly increased uterine wet weight and luminal epithelial height. Pretreatment with ICI 182,780 revealed ER-dependent activity of the compounds. In contrast to 17β-estradiol, genistein up-regulated ERα while coumestrol up-regulated both ERα and ERβ expr...

Applied Sciences, 2021

Endometriosis is characterized by unwanted growth of endometrial tissue in different locations of... more Endometriosis is characterized by unwanted growth of endometrial tissue in different locations of the female reproductive tract. It may lead to recurrent pregnancy loss, which is one of the worst curses for the reproductive age group of human populations around the world. Thus, there is an urgent need for unveiling any common source of origin of both these diseases and connections, if any. Herein, we aimed to identify common potential biomarker genes of these two diseases via in silico approach using meta-analysis of microarray data. Datasets were selected for the study based on certain exclusion criteria. Those datasets were subjected to comparative meta-analyses for the identification of differentially expressed genes (DEGs), that are common to both diagnoses. The DEGs were then subjected to protein-protein networking and subsequent functional enrichment analyses for unveiling their role/function in connecting two diseases. From the analyses, 120 DEGs are reported to be significan...

The present study is an attempt to investigate the phytochemical constituents and estimation of t... more The present study is an attempt to investigate the phytochemical constituents and estimation of total flavonoid and phenol contents of three different medicinal plant Desmodium caudatum, Streblus aspera and Plumeria acuminata that are used by the Mising tribes of Assam, India for curing reproductive related disorders. For phytochemical screening, some common and available standard tests were done. Aluminum chloride colorimetric method was used for total flavonoid determination using Quercetin as standard. Determination of total phenol was done by Folin-reagent method using Gallic acid as standard. Phytochemical screening showed the presence of active compounds in high concentration, such as alkaloids, flavonoids, phenolic compounds, saponin in addition to carbohydrate and protein. Among the three plant investigated, the total flavonoid content was found to be highest in S. aspera (971±12.41) μg/g quercetin equivalent of methanol extract in colorimetric method, than the other two; while the total phenol content was found to be highest in D. caudatum (97.00±3.46) μg/g gallic acid equivalent of methanol extract in Folin-reagent method. All these results show positive aspect of these plants to be used as medicine as these plant compounds are known to have different pharmacological activities.

Methanol extract (MLE) of the plant Nelsonia canescens (Lam..) Spreng at different doses (150, 30... more Methanol extract (MLE) of the plant Nelsonia canescens (Lam..) Spreng at different doses (150, 300 and 500 mg/kg, b.w) were tested for its efficacy against carbon tetrachloride (CCl4) induced acute hepatic damage in Wistar rats. The different groups of rats were administered with CCl4 (1ml/kg, s.c.). The rats were monitored for morphological changes, biochemical changes of serum Glutamate Oxaloacetate Transaminase(GOT), serum Glutamate Pyruvate Transaminase (GPT), serum Alkaline Phosphatase (ALP), serum Gamma Glutamyl Transferase (GGT), serum Cholesterol, serum Bilirubin (Total and Direct) and histopathological changes. From the experimental results it was proved that the plant showed a very good hepatoprotection in a dose dependent manner and the dose 500 mg/kg has significant effect in reducing the damage caused by CCl4 which was comparable to the protective effect of standard drug Silymarin (100 mg/kg, b.w). The phytochemical screening revealed the presence of active phytoconstit...

This study was conducted to compare six strains of eri silk worm, Samia ricini in terms of their ... more This study was conducted to compare six strains of eri silk worm, Samia ricini in terms of their growth and cocoon characters. The six strains of eri silk worms were identified morphologically and inbreeding was carried out for eight generations to get the six pure line strains. The six strains are Greenis h blue plain (GBP), Greenish blue spotted (GBS), Greenish blue zebra (GBZ), Yellow plain (YP), Yellow spotted (YS), Yellow zebra (YZ) were arranged into six groups. The growth of larva, silk glands, Silk Gland Tissue Somatic Index(SGTSI), cocoon shell weight, shell ratio of all the six strains were compared and it was found that a correlation is exist between the larval and silk gla nd weight from which silk thread results as well as with cocoon characters. At the end of the fifth instars measurements were performed of larvae nweight on a number of 20 individuals from each strain groups. After that silk glands were extracted with di ssection kit and their weight were determined by...

Journal of entomology and zoology studies, 2017

The present study was conducted to evaluate the haemolymph protein in six different Eri silk worm... more The present study was conducted to evaluate the haemolymph protein in six different Eri silk worm strains in their 5th instar- in the middle of the instar and at ripening or just before pupation. The result showed that in all the strains the concentration of protein was found highest at ripening when the worm is fully matured. Significant differences were found in protein level in two different study period of fifth instar larva in all six strains. Among all the strains Yellow Spotted (YS) was reported to have highest haemolymph protein concentration followed by Yellow Plain (YP). The protein concentration in ascending order was –GBZ

IOSR Journal of Pharmacy and Biological Sciences, 2017

Samia ricini, commonly known as eri silk worm is a domesticated, multivoltine, polyphagous silkwo... more Samia ricini, commonly known as eri silk worm is a domesticated, multivoltine, polyphagous silkworm species which have several morphotypes having distinct morphological differences. This silkworm species is cultured in different regions of Northeastern states specially in Assam. In addition to twenty six different ecoraces, six morphologically distinct strains of Samia ricini have been identified on the basis of larval markings and colour, they are Yellow plain (YP), Yellow spotted(YS), Yellow Zebra(YZ) and Greenish blue plain(GBP), Greenish blue spotted(GBS), Greenish blue zebra(GBZ).The present study was conducted with the aim to evaluate the rearing performance of these six strains of eri silk worm in four different seasons. To achieve this goal Fecundity, Hatching percent, Effective rate of rearing, larval duration and moth emergence and Shell ratio(%) were evaluated in spring, summer, autumn and winter. The study reported significant differences in all these parameters among different strains. The variation among them was significant at P <0.05 significant level. Seasonal variation also have profound effect on these parameters. Summer and winter seasons were reported as unfavorable in terms of performance while spring and autumn can be considered more suitable. Present study revealed that Yellow spotted strain showed highest shell ratio (%) therefore expected to produce more silk. ERR and hatching(%) was highest in GBP and GBS showed highest fecundity in autumn followed by spring. Larval duration was longest in GBS in winter and lowest in GBP in summer. The results revealed that Greenish blue strains-GBP and GBS are better in terms of rearing performance and spring and autumn are the favorable seasons for rearing of all six strains.

International Journal of Pharmacy and Pharmaceutical Sciences

ABSTRACT

Studies have revealed that there are numerous toxic chemicals available in the market since World... more Studies have revealed that there are numerous toxic chemicals available in the market since World War II which can act like natural hormone and fool the body's endocrine system. These endocrine disrupting chemicals or hormone mimics are called environmental estrogen or xenoestrogen. As many as 116 components have been identified as possible endocrine disrupting chemicals by European Commission in September 1999 out of which 50 endocrine disruptors including DDT, DDE, PCBs & even some pesticides like aldrin, dieldrin, heptachlor, kepone etc have been listed by WWF society. Environmental estrogens are ubiquitous in nature and are identified to be responsible for a number of physiological aberrations in human health as well as wildlife. A major area of concern has been in relation to the possible link between the effects of these chemicals and reported decline in sperm count along with the increasing reproductive disorders in living beings. This book particularly concentrates on in...

Methanolic extract of shade dried rhizome of Alpinia galanga (MAGE) was prepared using Soxhlet ap... more Methanolic extract of shade dried rhizome of Alpinia galanga (MAGE) was prepared using Soxhlet apparatus through a minimal of 18 hrs running. Working doses of the MAGE were determined after LD50 study selecting two doses of 200mg/kg Bwt/day and 500mg/kgBwt/day. The ovariectomised mice were randomly allocated into four groups (n=6) viz. vehicle control (1% tween 80), positive control (E2), low dose MAGE (200mg) and high dose MAGE (500mg). After 7 days of oral treatment, animals were sacrificed by cervical dislocation. Sample tissues were carefully collected and weighted using Sartorius balance. Morphological observations revealed that E2¬ treated group showed positive sign of exogenous estrogenic activity by increasing uterine wet weight and relative increase in uterine/body weight ratio. While low dose MAGE treated group shows weak estrogenic activity by increasing uterine wet weight and uterine/body weight, but less pronounced than E2 treated group. The high dose MAGE treated group...

The methanol extract (MLE) of the plant Nelsonia canescens (Lam.) Spreng at different doses (150,... more The methanol extract (MLE) of the plant Nelsonia canescens (Lam.) Spreng at different doses (150, 300 and 500 mg/kg, b.w.) was tested for its efficacy against paracetamol induced acute hepatic damage in Wistar rats. The different groups of rats were administered with paracetamol (2 gm/kg, p.o.). The rats were monitored for morphological changes, biochemical changes of serum Glutamate Oxaloacetate Transaminase (GOT), serum Glutamate Pyruvate Transaminase (GPT), serum Alkaline Phosphatase (ALP), serum Gamma Glutamyl Transferase (GGT), serum Cholesterol, serum Bilirubin (Total and Direct) and for histopathological changes. Activity of the MLE on paracetamol induced lethal effect in mice was also studied. From the experimental results it was proved that the plant possesses hepatoprotective potency in a dose dependent manner and the dose 500 mg/kg has significant effect in reducing the damage caused by paracetamol; which was comparable to the protective effect of standard drug Silymarin (100 mg/kg, b.w.). The phytochemical screening revealed the presence of active phytoconstituents i.e. flavanoids and phenolics, which may offer hepatoprotection. The present work support the traditional claim of plant in the treatment of liver injury, may provide a new drug against a war with liver diseases.

ASSESSMENT, 2011

The present study for the first time examined the fish diversity status of the Koya Kujiya Beel i... more The present study for the first time examined the fish diversity status of the Koya Kujiya Beel in the Goalpara district of Assam (North-East India). The study investigated the parameters related to the physico-chemical nature of the beel and found that the Koya ...