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Papers by Kalyani Mohanram

Vaccines

Global vaccine development efforts have been accelerated in response to the devastating COVID-19 ... more Global vaccine development efforts have been accelerated in response to the devastating COVID-19 pandemic. The study aims to determine the seroprevalence of SARS-CoV-2 IgG antibodies among vaccine-naïve healthcare workers and to describe the impact of vaccination roll-out on COVID-19 antibody prevalence among the health care centers in tertiary care centers in South India. Serum samples collected from vaccinated and unvaccinated health care workers between January 2021 and April 2021were subjected to COVID-19 IgG ELISA, and adverse effects after the first and second dose of receiving the Covishield vaccine were recorded. The vaccinated group was followed for a COVID-19 breakthrough infection for a period of 6 months. Among the recruited HCW, 156 and 157 participants were from the vaccinated and unvaccinated group, respectively. The seroprevalence (COVID-19 IgG ELISA) among the vaccinated and unvaccinated Health Care Workers (HCW) was 91.7% and 38.2%, respectively, which is statistic...

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2015

Journal of Medical Science And clinical Research, 2016

The emerging resistance to Carbapenems which are generally considered as life saving drugs to tre... more The emerging resistance to Carbapenems which are generally considered as life saving drugs to treat infections caused by ESBL and AmpC producing bacteria, has become a serious issue worldwide. It is therefore necessary to detect Carbapenemases to limit the spread of multidrug resistant organisms for effective Antibiotic surveillance and Infection Control in the Hospital. Aim & Objectives: To detect the presence of MBL, KPC Carbapenemase and their CoExistence among the Carbapenem resistant clinical isolates of Gram Negative Bacilli Materials & Methods: The present study was carried out in a Tertiary care hospital; to detect Carbapenamases among Gram Negative Bacilli by Inhibitor based combined Disc tests in which Phenylboronic Acid and Dipicolinic acid are incorporated onto Meropenem discs. Results: A Total of 718 strains of Gram Negative Bacilli comprising of 516 strains of Enterobacteriaceae and 202 Non-fermenters were included in the study. Out of these 718 strains, 89 strains were resistant to carbapenems, of which 2.5% (18 /718) were KPC (Klebsiella Pneumoniae Carbapenemase-class A) producers, 8.08%(58/718) were MBL (Metallo Beta Lactamases-class B) producers .Co-existence of MBL and KPC was observed in 1.25% (9 /718) of isolates and no mechanism was detected in 4 isolates. Conclusion: Inhibitor based combined disc test is simple and cost effective phenotypic test for detecting Carbapenem resistance in the Laboratory. Antibiotic stewardship programme has to be implemented in Hospital to achieve good Infection control for better patient outcome and reduce the health care costs.

Klebsiella pneumoniae, Gram Negative, capsulated rod shaped Bacilli is responsible for causing li... more Klebsiella pneumoniae, Gram Negative, capsulated rod shaped Bacilli is responsible for causing life threatening infections in humans and also opportunistic bacterial pathogen of clinical relevance for its association with both nosocomial infections and community acquired infections.Moreover, increased resistance to these antibiotics complicates and limits the available therapeutic drug options for the clinicians. Hence this study was taken to investigate the antimicrobial drug resistance and Prevalence of ESBL producing Klebsiella pneumoniae. This is a Retrospective study conducted at from JANUARY 2018 to DECEMBER 2018 at Saveetha Medical College and Hospital included Sputum sample of 3125 in patients from various Department, out of which 2154 had growth, from which 1320 Klebsiella pneumoniae isolates were collected. Patient’s demographic details, co-morbid conditions other associated risk factors were also collected. Antibiotic susceptibility testing (AST) was done on Muller Hinton...

Introduction: Candida is a genus of yeast which is the most common cause of fungal infection. Can... more Introduction: Candida is a genus of yeast which is the most common cause of fungal infection. Candida albicans is most important fungal opportunistic pathogen. The candiduria refers to the presence of yeast in urine either by the microscope observation of budding pseudohyphae, or by the growth of fungi in culture. Aim: To find the prevalence of candiduria in tertiary care Centre. Materials and Method: This was a study conducted at Saveetha medical college from January 2018 to December 2018, the total number of urine sample collected were 10194 out of which 2500 showed various microbial growth, out of which 50 Candida strains were isolated. Speciation was done according to standard protocol. Result: The prevalence of candiduria caused by Candida non-albicans species (1.24%) is more than that caused by Candida albicans species (0.76%). The prevalence is more in female (66%) than male (34%). Among the age group the prevalence is more in the age group between 21-30 years (50%). Candidur...

Current Medical Mycology

Background and Purpose: Azoles are preferred antifungal agents given their inexpensiveness, limit... more Background and Purpose: Azoles are preferred antifungal agents given their inexpensiveness, limited toxicity, and potentiality of oral administration. However, the extensive use of prophylactic azole therapy for chronic infections, especially in immunocompromised patients, has led to an increase in azole resistance, thereby rising health care costs. Fluconazole resistance is associated with poor clinical outcomes and the emergence of new infections. The present study aimed to investigate the mutations of ERG11 gene in fluconazole-resistant Candida albicans isolates. Materials and Methods: This study was conducted on 80 clinical samples collected from HIV-infected patients with suspected candidiasis in Tagore Medical College Hospital and Government Hospital of Thoracic Medicine, Chennai, India, for a period of 18 months (May 2016-December 2017). The antifungal susceptibility pattern was determined by agar diffusion and broth dilution techniques as per the Clinical and Laboratory Stan...

AYU (An international quarterly journal of research in Ayurveda)

Current pharmaceutical biotechnology, 2018

Candida species is the common cause of opportunistic fungal infections all over the world with in... more Candida species is the common cause of opportunistic fungal infections all over the world with increased mortality and morbidity especially in immunosuppressed patients. Fluconazole is the first line therapy for candidiasis. The antifungal resistance pattern in high-risk patients is a major concern. The present study was aimed to assess the anticandidal activity of Punica granatum peel against fluconazole resistant Candida species isolated from HIV patients. Ethanol, chloroform, petroleum ether and aqueous extracts of the peel of P. granatum were evaluated against standard strains of Candida spp. and fluconazole resistant clinical isolates by agar diffusion and broth dilution techniques. The GC-MS analysis of the extracts was performed to identify the phytochemicals present in it. The predominant phytochemical was subjected to molecular docking study to determine its binding efficacy with lanosterol 14-alpha demethylase. P. granatum peel extracts showed excellent anticandidal activi...

Mycopathologia, Jan 21, 2017

The genus Tritirachium is a mitosporic fungus which inhabits in soil and decaying plant material ... more The genus Tritirachium is a mitosporic fungus which inhabits in soil and decaying plant material and also a notable insect pathogen. Human infections with Tritirachium species though rare were previously reported to cause corneal ulcers, otomycosis, onychomycosis, and dermatomycosis of the scalp and hence may be considered as a potential pathogen. Here we report a case of distal lateral subungual onychomycosis involving right great toenail in a 22-year-old female, wherein direct potassium hydroxide preparations, fungal cultures, and molecular sequencing of the isolate established Tritirachium oryzae as the etiological agent. Antifungal susceptibility performed by the microbroth technique of CLSI revealed increased MICs to amphotericin B and low MICs to azoles and echinocandins. The case was managed with surgical nail avulsion followed by topical application of 2% ketoconazole cream resulting regrowth of normal nail. To the best of our knowledge, this is the first report of non-derma...

International Journal of Infertility & Fetal Medicine

Aim and objective: The present study determines the incidence of anti-Chlamydia antibodies (ACA),... more Aim and objective: The present study determines the incidence of anti-Chlamydia antibodies (ACA), antisperm antibody (ASA), and thyroid levels in serum of infertility patients and statistically determines the significance of all three study parameters. Materials and methods: A cross-sectional study was conducted on volunteers of 190 patients both male and female reporting to the Infertility Clinic of hospital and 178 patients including pregnant women patients were used as control. Serum from patients was collected and analyzed for the presence of anti-Chlamydia antibodies, ASA, serum T3, T4, and thyroid-stimulating hormone (TSH) levels by enzyme-linked immunosorbent assay (ELISA). Results: Infertility patients representing 12.6% of the study population tested positive for anti-Chlamydia antibodies. 21.6% of 190 patients had high ASA levels and these levels were found to be statistically significant p < 0.001 when compared with control. Elevated thyroid levels of the study population observed as increased T3 in 26 patients and T4 in 18 patients with 16 patients having increased TSH and the increased levels of thyroid in infertility patients compared with control is statistically significant p value <0.01. Correlation between all three study parameters is statistically significant [0.01 level (two-tailed)]. Conclusion: Significantly higher levels of anti-Chlamydia antibodies and ASA are found associated in infertile patients as is elevated T3, T4, and TSH. All three parameters together can cause infertility showing the multidimensional factors for the development of infertility. Clinical significance: Patients with infertility should be screened for all three study parameters viz. ACA, ASA, and thyroid levels and subsequent treatment protocols to resolve all possible adverse results to within normal levels for the success of ART. Research highlights • The present study found higher levels of anti-Chlamydia antibody in patients with primary infertility compared with the control group indicating the strong association between chlamydial infections and infertility. • The present study found higher levels of antisperm antibodies in the serum of patients with primary infertility compared with the control group implying a significant role for ASA and development of infertility. • Thyroid disorders such as hypothyroidism due to elevated TSH and hyperthyroidism associated with increased total serum T3 or T4 are several times higher in infertility patients when compared with the general population providing evidence for thyroid imbalance and infertility. • All three study parameters-anti-Chlamydia antibodies, ASA, and thyroid levels occurring together in any one patient may tend to cause infertility.

International Journal of Research in Pharmaceutical Sciences

This study was conducted with interest in increasing carbapenem resistance in non-fermenters: an ... more This study was conducted with interest in increasing carbapenem resistance in non-fermenters: an important causative agent of nosocomial infection and to standardize the methods for interpretation of their resistance. The aim of this study is to perform disk diffusion testing and minimal inhibitory concentration technique for the identification of carbapenem resistance for imipenem and meropenem. The isolates found resistant to carbapenems were confirmed with the modified Hodge test. The genes responsible for carbapenem resistance were identified by both phenotypic and genotypic methods. Out of 240 non-fermenters isolated 20% showed resistance to carbapenem by disk diffusion. Only 7% showed resistance by the micro broth dilution technique of minimum inhibitory concentration. 3% were panning drug-resistant. Out of 16 carbapenem-resistant isolates, 5 were found to have KPC (Klebsiella pneumonia carbapenem) genes, 9 had MBL (Metallo beta-lactamase) genes and 2 had KPC+MBL genes and non...

Saudi Journal of Pathology and Microbiology

Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are ... more Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are inevitably exposed to a large volume of water during hemodialysis. During this process the semi-permeable artificial membrane comes into direct contact with the bloodstream. Therefore it is important to monitor the purity of dialysis water. Aim and objectives: To compare two different methods for dialysis water analysis. Material and methods: 50 samples of dialysis water were collected from Saveetha Medical College and Hospital for this study. The study was conducted from 2018 December to 2019 March after getting Institutional Human Ethical Committee (IHEC) Clearance. By using the criteria of the AAMI, the present study is taken up to analyse the sensitivity of two different cultures technique i.e., spread plate and membrane filteration technique. Results: The standardization shows that spread plate technique was 80% effective and membrane filtration technique was 70% effective in identifying 100 CFU/mL of bacteria tested. Out of 50 unknown samples tested, 2 were ultra-pure, 21 were between 0.1-50 CFU/mL, 16 were between 50-100 CFU/mL and 11 were >100 CFU/mL by Spread plate technique. Likewise, 6 were ultra-pure, 30 were between 0.1-50 CFU/mL, 10 were between 50-100 CFU/mL and 4 were >100 CFU/mL by Membrane Filtration technique. Conclusion: From this study, spread plate technique proves to be equally sensitive with membrane filtration technique for analyzing dialysis water but when ultrapure water needs to be analyzed, spread plate technique gives much better bacterial recovery. i. e. , on l y 2 samples were proved to be ultra-pure by Spread plate technique.

Saudi Journal of Pathology and Microbiology, Sep 30, 2019

Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are ... more Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are inevitably exposed to a large volume of water during hemodialysis. During this process the semi-permeable artificial membrane comes into direct contact with the bloodstream. Therefore it is important to monitor the purity of dialysis water. Aim and objectives: To compare two different methods for dialysis water analysis. Material and methods: 50 samples of dialysis water were collected from Saveetha Medical College and Hospital for this study. The study was conducted from 2018 December to 2019 March after getting Institutional Human Ethical Committee (IHEC) Clearance. By using the criteria of the AAMI, the present study is taken up to analyse the sensitivity of two different cultures technique i.e., spread plate and membrane filteration technique. Results: The standardization shows that spread plate technique was 80% effective and membrane filtration technique was 70% effective in identifying 100 CFU/mL of bacteria tested. Out of 50 unknown samples tested, 2 were ultra-pure, 21 were between 0.1-50 CFU/mL, 16 were between 50-100 CFU/mL and 11 were >100 CFU/mL by Spread plate technique. Likewise, 6 were ultra-pure, 30 were between 0.1-50 CFU/mL, 10 were between 50-100 CFU/mL and 4 were >100 CFU/mL by Membrane Filtration technique. Conclusion: From this study, spread plate technique proves to be equally sensitive with membrane filtration technique for analyzing dialysis water but when ultrapure water needs to be analyzed, spread plate technique gives much better bacterial recovery. i. e. , on l y 2 samples were proved to be ultra-pure by Spread plate technique.

Cureus, Jan 20, 2018

Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving ... more Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving the skin of the face but not the mustache and beard (Tinea barbae). It poses a diagnostic dilemma with its atypical clinical presentation. Pityriasis folliculorum (PF) is a dermatological condition that results in rosacea-like skin eruptions. It was previously associated with a human ectoparasitic infestation. mites () is a group of obligate parasites that live on the skin of mammals. These mites have been associated with various dermatological disorders, clinically termed as demodicosis. Insects have been described as potential vectors that can carry various microorganisms and especially spores of fungi. Hence, infestation by such insects may aggravate the already present skin condition, leading to secondary infections. There has been a change in the trend of dermatophytosis worldwide and infections caused by (. ) are increasing. Hence, there is an urgent need for a thorough investigati...

Journal of Pure and Applied Microbiology

Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving ... more Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving the skin of the face but not the mustache and beard (Tinea barbae). It poses a diagnostic dilemma with its atypical clinical presentation. Pityriasis folliculorum (PF) is a dermatological condition that results in rosacea-like skin eruptions. It was previously associated with a human ectoparasitic infestation. Demodex mites (Demodex folliculorum) is a group of obligate parasites that live on the skin of mammals. These mites have been associated with various dermatological disorders, clinically termed as demodicosis. Insects have been described as potential vectors that can carry various microorganisms and especially spores of fungi. Hence, infestation by such insects may aggravate the already present skin condition, leading to secondary infections. There has been a change in the trend of dermatophytosis worldwide and infections caused by Trichophyton mentagrophytes var. interdigitale (T. interdigitale) are increasing. Hence, there is an urgent need for a thorough investigation of an infectious etiology among various skin disorders. This is the first report of concomitant Tinea faciei and Pityriasis folliculorum involving facial skin.

Vaccines

Global vaccine development efforts have been accelerated in response to the devastating COVID-19 ... more Global vaccine development efforts have been accelerated in response to the devastating COVID-19 pandemic. The study aims to determine the seroprevalence of SARS-CoV-2 IgG antibodies among vaccine-naïve healthcare workers and to describe the impact of vaccination roll-out on COVID-19 antibody prevalence among the health care centers in tertiary care centers in South India. Serum samples collected from vaccinated and unvaccinated health care workers between January 2021 and April 2021were subjected to COVID-19 IgG ELISA, and adverse effects after the first and second dose of receiving the Covishield vaccine were recorded. The vaccinated group was followed for a COVID-19 breakthrough infection for a period of 6 months. Among the recruited HCW, 156 and 157 participants were from the vaccinated and unvaccinated group, respectively. The seroprevalence (COVID-19 IgG ELISA) among the vaccinated and unvaccinated Health Care Workers (HCW) was 91.7% and 38.2%, respectively, which is statistic...

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2015

Journal of Medical Science And clinical Research, 2016

The emerging resistance to Carbapenems which are generally considered as life saving drugs to tre... more The emerging resistance to Carbapenems which are generally considered as life saving drugs to treat infections caused by ESBL and AmpC producing bacteria, has become a serious issue worldwide. It is therefore necessary to detect Carbapenemases to limit the spread of multidrug resistant organisms for effective Antibiotic surveillance and Infection Control in the Hospital. Aim & Objectives: To detect the presence of MBL, KPC Carbapenemase and their CoExistence among the Carbapenem resistant clinical isolates of Gram Negative Bacilli Materials & Methods: The present study was carried out in a Tertiary care hospital; to detect Carbapenamases among Gram Negative Bacilli by Inhibitor based combined Disc tests in which Phenylboronic Acid and Dipicolinic acid are incorporated onto Meropenem discs. Results: A Total of 718 strains of Gram Negative Bacilli comprising of 516 strains of Enterobacteriaceae and 202 Non-fermenters were included in the study. Out of these 718 strains, 89 strains were resistant to carbapenems, of which 2.5% (18 /718) were KPC (Klebsiella Pneumoniae Carbapenemase-class A) producers, 8.08%(58/718) were MBL (Metallo Beta Lactamases-class B) producers .Co-existence of MBL and KPC was observed in 1.25% (9 /718) of isolates and no mechanism was detected in 4 isolates. Conclusion: Inhibitor based combined disc test is simple and cost effective phenotypic test for detecting Carbapenem resistance in the Laboratory. Antibiotic stewardship programme has to be implemented in Hospital to achieve good Infection control for better patient outcome and reduce the health care costs.

Klebsiella pneumoniae, Gram Negative, capsulated rod shaped Bacilli is responsible for causing li... more Klebsiella pneumoniae, Gram Negative, capsulated rod shaped Bacilli is responsible for causing life threatening infections in humans and also opportunistic bacterial pathogen of clinical relevance for its association with both nosocomial infections and community acquired infections.Moreover, increased resistance to these antibiotics complicates and limits the available therapeutic drug options for the clinicians. Hence this study was taken to investigate the antimicrobial drug resistance and Prevalence of ESBL producing Klebsiella pneumoniae. This is a Retrospective study conducted at from JANUARY 2018 to DECEMBER 2018 at Saveetha Medical College and Hospital included Sputum sample of 3125 in patients from various Department, out of which 2154 had growth, from which 1320 Klebsiella pneumoniae isolates were collected. Patient’s demographic details, co-morbid conditions other associated risk factors were also collected. Antibiotic susceptibility testing (AST) was done on Muller Hinton...

Introduction: Candida is a genus of yeast which is the most common cause of fungal infection. Can... more Introduction: Candida is a genus of yeast which is the most common cause of fungal infection. Candida albicans is most important fungal opportunistic pathogen. The candiduria refers to the presence of yeast in urine either by the microscope observation of budding pseudohyphae, or by the growth of fungi in culture. Aim: To find the prevalence of candiduria in tertiary care Centre. Materials and Method: This was a study conducted at Saveetha medical college from January 2018 to December 2018, the total number of urine sample collected were 10194 out of which 2500 showed various microbial growth, out of which 50 Candida strains were isolated. Speciation was done according to standard protocol. Result: The prevalence of candiduria caused by Candida non-albicans species (1.24%) is more than that caused by Candida albicans species (0.76%). The prevalence is more in female (66%) than male (34%). Among the age group the prevalence is more in the age group between 21-30 years (50%). Candidur...

Current Medical Mycology

Background and Purpose: Azoles are preferred antifungal agents given their inexpensiveness, limit... more Background and Purpose: Azoles are preferred antifungal agents given their inexpensiveness, limited toxicity, and potentiality of oral administration. However, the extensive use of prophylactic azole therapy for chronic infections, especially in immunocompromised patients, has led to an increase in azole resistance, thereby rising health care costs. Fluconazole resistance is associated with poor clinical outcomes and the emergence of new infections. The present study aimed to investigate the mutations of ERG11 gene in fluconazole-resistant Candida albicans isolates. Materials and Methods: This study was conducted on 80 clinical samples collected from HIV-infected patients with suspected candidiasis in Tagore Medical College Hospital and Government Hospital of Thoracic Medicine, Chennai, India, for a period of 18 months (May 2016-December 2017). The antifungal susceptibility pattern was determined by agar diffusion and broth dilution techniques as per the Clinical and Laboratory Stan...

AYU (An international quarterly journal of research in Ayurveda)

Current pharmaceutical biotechnology, 2018

Candida species is the common cause of opportunistic fungal infections all over the world with in... more Candida species is the common cause of opportunistic fungal infections all over the world with increased mortality and morbidity especially in immunosuppressed patients. Fluconazole is the first line therapy for candidiasis. The antifungal resistance pattern in high-risk patients is a major concern. The present study was aimed to assess the anticandidal activity of Punica granatum peel against fluconazole resistant Candida species isolated from HIV patients. Ethanol, chloroform, petroleum ether and aqueous extracts of the peel of P. granatum were evaluated against standard strains of Candida spp. and fluconazole resistant clinical isolates by agar diffusion and broth dilution techniques. The GC-MS analysis of the extracts was performed to identify the phytochemicals present in it. The predominant phytochemical was subjected to molecular docking study to determine its binding efficacy with lanosterol 14-alpha demethylase. P. granatum peel extracts showed excellent anticandidal activi...

Mycopathologia, Jan 21, 2017

The genus Tritirachium is a mitosporic fungus which inhabits in soil and decaying plant material ... more The genus Tritirachium is a mitosporic fungus which inhabits in soil and decaying plant material and also a notable insect pathogen. Human infections with Tritirachium species though rare were previously reported to cause corneal ulcers, otomycosis, onychomycosis, and dermatomycosis of the scalp and hence may be considered as a potential pathogen. Here we report a case of distal lateral subungual onychomycosis involving right great toenail in a 22-year-old female, wherein direct potassium hydroxide preparations, fungal cultures, and molecular sequencing of the isolate established Tritirachium oryzae as the etiological agent. Antifungal susceptibility performed by the microbroth technique of CLSI revealed increased MICs to amphotericin B and low MICs to azoles and echinocandins. The case was managed with surgical nail avulsion followed by topical application of 2% ketoconazole cream resulting regrowth of normal nail. To the best of our knowledge, this is the first report of non-derma...

International Journal of Infertility & Fetal Medicine

Aim and objective: The present study determines the incidence of anti-Chlamydia antibodies (ACA),... more Aim and objective: The present study determines the incidence of anti-Chlamydia antibodies (ACA), antisperm antibody (ASA), and thyroid levels in serum of infertility patients and statistically determines the significance of all three study parameters. Materials and methods: A cross-sectional study was conducted on volunteers of 190 patients both male and female reporting to the Infertility Clinic of hospital and 178 patients including pregnant women patients were used as control. Serum from patients was collected and analyzed for the presence of anti-Chlamydia antibodies, ASA, serum T3, T4, and thyroid-stimulating hormone (TSH) levels by enzyme-linked immunosorbent assay (ELISA). Results: Infertility patients representing 12.6% of the study population tested positive for anti-Chlamydia antibodies. 21.6% of 190 patients had high ASA levels and these levels were found to be statistically significant p < 0.001 when compared with control. Elevated thyroid levels of the study population observed as increased T3 in 26 patients and T4 in 18 patients with 16 patients having increased TSH and the increased levels of thyroid in infertility patients compared with control is statistically significant p value <0.01. Correlation between all three study parameters is statistically significant [0.01 level (two-tailed)]. Conclusion: Significantly higher levels of anti-Chlamydia antibodies and ASA are found associated in infertile patients as is elevated T3, T4, and TSH. All three parameters together can cause infertility showing the multidimensional factors for the development of infertility. Clinical significance: Patients with infertility should be screened for all three study parameters viz. ACA, ASA, and thyroid levels and subsequent treatment protocols to resolve all possible adverse results to within normal levels for the success of ART. Research highlights • The present study found higher levels of anti-Chlamydia antibody in patients with primary infertility compared with the control group indicating the strong association between chlamydial infections and infertility. • The present study found higher levels of antisperm antibodies in the serum of patients with primary infertility compared with the control group implying a significant role for ASA and development of infertility. • Thyroid disorders such as hypothyroidism due to elevated TSH and hyperthyroidism associated with increased total serum T3 or T4 are several times higher in infertility patients when compared with the general population providing evidence for thyroid imbalance and infertility. • All three study parameters-anti-Chlamydia antibodies, ASA, and thyroid levels occurring together in any one patient may tend to cause infertility.

International Journal of Research in Pharmaceutical Sciences

This study was conducted with interest in increasing carbapenem resistance in non-fermenters: an ... more This study was conducted with interest in increasing carbapenem resistance in non-fermenters: an important causative agent of nosocomial infection and to standardize the methods for interpretation of their resistance. The aim of this study is to perform disk diffusion testing and minimal inhibitory concentration technique for the identification of carbapenem resistance for imipenem and meropenem. The isolates found resistant to carbapenems were confirmed with the modified Hodge test. The genes responsible for carbapenem resistance were identified by both phenotypic and genotypic methods. Out of 240 non-fermenters isolated 20% showed resistance to carbapenem by disk diffusion. Only 7% showed resistance by the micro broth dilution technique of minimum inhibitory concentration. 3% were panning drug-resistant. Out of 16 carbapenem-resistant isolates, 5 were found to have KPC (Klebsiella pneumonia carbapenem) genes, 9 had MBL (Metallo beta-lactamase) genes and 2 had KPC+MBL genes and non...

Saudi Journal of Pathology and Microbiology

Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are ... more Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are inevitably exposed to a large volume of water during hemodialysis. During this process the semi-permeable artificial membrane comes into direct contact with the bloodstream. Therefore it is important to monitor the purity of dialysis water. Aim and objectives: To compare two different methods for dialysis water analysis. Material and methods: 50 samples of dialysis water were collected from Saveetha Medical College and Hospital for this study. The study was conducted from 2018 December to 2019 March after getting Institutional Human Ethical Committee (IHEC) Clearance. By using the criteria of the AAMI, the present study is taken up to analyse the sensitivity of two different cultures technique i.e., spread plate and membrane filteration technique. Results: The standardization shows that spread plate technique was 80% effective and membrane filtration technique was 70% effective in identifying 100 CFU/mL of bacteria tested. Out of 50 unknown samples tested, 2 were ultra-pure, 21 were between 0.1-50 CFU/mL, 16 were between 50-100 CFU/mL and 11 were >100 CFU/mL by Spread plate technique. Likewise, 6 were ultra-pure, 30 were between 0.1-50 CFU/mL, 10 were between 50-100 CFU/mL and 4 were >100 CFU/mL by Membrane Filtration technique. Conclusion: From this study, spread plate technique proves to be equally sensitive with membrane filtration technique for analyzing dialysis water but when ultrapure water needs to be analyzed, spread plate technique gives much better bacterial recovery. i. e. , on l y 2 samples were proved to be ultra-pure by Spread plate technique.

Saudi Journal of Pathology and Microbiology, Sep 30, 2019

Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are ... more Introduction: The purity of the hemodialysis fluids is crucial for hemodialysis patients who are inevitably exposed to a large volume of water during hemodialysis. During this process the semi-permeable artificial membrane comes into direct contact with the bloodstream. Therefore it is important to monitor the purity of dialysis water. Aim and objectives: To compare two different methods for dialysis water analysis. Material and methods: 50 samples of dialysis water were collected from Saveetha Medical College and Hospital for this study. The study was conducted from 2018 December to 2019 March after getting Institutional Human Ethical Committee (IHEC) Clearance. By using the criteria of the AAMI, the present study is taken up to analyse the sensitivity of two different cultures technique i.e., spread plate and membrane filteration technique. Results: The standardization shows that spread plate technique was 80% effective and membrane filtration technique was 70% effective in identifying 100 CFU/mL of bacteria tested. Out of 50 unknown samples tested, 2 were ultra-pure, 21 were between 0.1-50 CFU/mL, 16 were between 50-100 CFU/mL and 11 were >100 CFU/mL by Spread plate technique. Likewise, 6 were ultra-pure, 30 were between 0.1-50 CFU/mL, 10 were between 50-100 CFU/mL and 4 were >100 CFU/mL by Membrane Filtration technique. Conclusion: From this study, spread plate technique proves to be equally sensitive with membrane filtration technique for analyzing dialysis water but when ultrapure water needs to be analyzed, spread plate technique gives much better bacterial recovery. i. e. , on l y 2 samples were proved to be ultra-pure by Spread plate technique.

Cureus, Jan 20, 2018

Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving ... more Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving the skin of the face but not the mustache and beard (Tinea barbae). It poses a diagnostic dilemma with its atypical clinical presentation. Pityriasis folliculorum (PF) is a dermatological condition that results in rosacea-like skin eruptions. It was previously associated with a human ectoparasitic infestation. mites () is a group of obligate parasites that live on the skin of mammals. These mites have been associated with various dermatological disorders, clinically termed as demodicosis. Insects have been described as potential vectors that can carry various microorganisms and especially spores of fungi. Hence, infestation by such insects may aggravate the already present skin condition, leading to secondary infections. There has been a change in the trend of dermatophytosis worldwide and infections caused by (. ) are increasing. Hence, there is an urgent need for a thorough investigati...

Journal of Pure and Applied Microbiology

Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving ... more Tinea faciei (TF) is a common dermatomicrobiological condition caused by dermatophytes involving the skin of the face but not the mustache and beard (Tinea barbae). It poses a diagnostic dilemma with its atypical clinical presentation. Pityriasis folliculorum (PF) is a dermatological condition that results in rosacea-like skin eruptions. It was previously associated with a human ectoparasitic infestation. Demodex mites (Demodex folliculorum) is a group of obligate parasites that live on the skin of mammals. These mites have been associated with various dermatological disorders, clinically termed as demodicosis. Insects have been described as potential vectors that can carry various microorganisms and especially spores of fungi. Hence, infestation by such insects may aggravate the already present skin condition, leading to secondary infections. There has been a change in the trend of dermatophytosis worldwide and infections caused by Trichophyton mentagrophytes var. interdigitale (T. interdigitale) are increasing. Hence, there is an urgent need for a thorough investigation of an infectious etiology among various skin disorders. This is the first report of concomitant Tinea faciei and Pityriasis folliculorum involving facial skin.