Karkaz Thalij - Academia.edu (original) (raw)

Papers by Karkaz Thalij

Tikrit Journal for Agricultural Sciences, Dec 30, 2023

The research aimed to prepare the nanoparticles of zinc, vitamin D3 and cysteine with selenium na... more The research aimed to prepare the nanoparticles of zinc, vitamin D3 and cysteine with selenium nanoparticles (Se-NPs), and to determine the effect of each of them in inhibiting both the isolated Escherichia coli and Staphylococcus aureus isolated from food by estimating the minimum inhibitory concentration (MIC) with Kirby Bauer disk diffusion. The results showed that the conjugations of zinc, vitamin D3 and cysteine with se-NPs were more effective in bacterial inhibition compared to inhibition of Se-NPs alone. The MIC of Se-NPs alone or in combination with Zn, D3 or Cysteine against both species of bacteria was appeared at 1% and above. The inhibitory activity increased against the bacterial species E.coli and S. aureus as the concentrations used in the treatments were increased, while the diameter of the inhibition zone was 25% against the two types of bacteria E.coli and Staph. aureus was the highest effect when treated with Se Nps + D3, which was at 30 mm, and 22 mm, respectively.

Aquaculture Reports

ALP, LDH as compared to other group (P < 0.05). In conclusion, a combined administration o... more ALP, LDH as compared to other group (P < 0.05). In conclusion, a combined administration of RE and LAB effectively improved growth performance and health status as well as protected common carp against crowding stress.

International Journal of Biological Macromolecules

ANBAR JOURNAL OF AGRICULTURAL SCIENCES, 2017

Tikrit Journal for Agricultural Sciences, 2018

Mağallaẗ Tikrīt li-l-ʻulūm al-zirāʻaẗ̈, Dec 31, 2021

The Genomic DNA had been extracted from the liver tissue of the experimental rat groups following... more The Genomic DNA had been extracted from the liver tissue of the experimental rat groups following 15 weeks of treatment with partial purified L-glutaminase enzyme from E.coli in relation with ethylamine. The DNA samples of all the six treatment groups were amplified by PCR using three different primers specific for determining the presence of P53, bax and G3pdh genes and detect the effect of Ethylenimine and L-glutaminase on the animals at the molecular level. The agarose gel electrophoresis technique used to analyze the PCR amplification products. The result revealed the presence of P53 gene in all treatment groups except for diseased group (T2). This finding demonstrates the mutagenic effect of Ethylenimine that lead to mutation at P53 gene sequence, and therapeutic beneficial of L-glutaminase. Also, there is no PCR amplification product which represent Bax gene sequence for T2 and T5 groups which were administered doses of Ethylenimine, indicating that low doses of L-glutaminase failed to prevent the mutagenic effect of Ethylenimine. While, the G3pd genes is presented only in T2 and T5. Finally, analysis the DNA sequences of the PCR amplified products extracted from liver samples of T2 group treated with Ethylenimine was carried out, then the results were compared with NCBI. The expected mutations were found at thirteen locals and there were only three mutated sequences with the DNA of liver samples from T5 group, while the DNA of animals in group (T6) were showed no mutated regions. The results of this novel study make clear the therapeutic effect of L-glutaminase and how suppress the mutagenic and carcinogenic effect of ethylenimine on P53, Bax, and G3pd genes, and its effect was dose dependent..

Acetamiprid is one of the most wide used insecticides over the entire world. Due to the widesprea... more Acetamiprid is one of the most wide used insecticides over the entire world. Due to the widespread use of flavonoids in the treatment of many disease, The aim of this study was to see if Quercetin and Hesperidin could help reduce the effects of Acetamiprid on biochemical parameters which include Malondialdehyde, Aspartate aminotransferase, Alanine aminotransferase, Creatinine, and Urea, as well as antioxidants like Superoxide dismutase, Glutathion-S-transferase, Catalase and expression level of MAPK8 and NFKB genes. A total of 48 male rats were used in the investigation, with each group consisting of six animals that were dosed for 30 days. The groups were divided as follows: Control group (C) given drinking water only, (T1) given orally Acetamiprid in the dose (40mg/kg/B.W),(T2) given orally Quercetin in dose (20mg/kg/B.W),(T3) given orally Hesperidin in the dose (25mg/kg/B.W), (T4) given Quercetin in the dose(20mg/kg/B.W) then Hesperidin in the dose (25mg/kg/B.W)Concurrently,(T5) given Acetamiprid e in the dose (40mg/kg/B.W) then given Quercetin in the dose(20mg/kg/B.W) Concurrently, (T6) given Acetamiprid in the dose (40mg/kg/B.W) then given Hesperidin in the dose(25mg/kg/B.W) Concurrently, and(T7) given Acetamiprid in dose (40mg/kg/B.W) then given Quercetin in the dose(20mg/kg/B.W) then given Hesperidin in the dose(25mg/kg/B.W) Concurrently. The results clarified a significant increase (P<0.05) in Malondialdehyde, Urea, Creatinine, liver enzymes(AST,ALT) and gene expression of MAPK8 and NFKB genes, and a significant decrease in the level of enzymatic antioxidants (SOD, GST, CAT) in (T1) compared to (C) and other groups. On the other hand, results showed that giving animals Quercetin and Hesperidin separately or both simultaneously with the pesticide Acetamiprid in treatments (T5, T6, T7)caused of a significant improvement in the studied parameters compared to (T1) that was dosed with the pesticide alone. It was sometimes close to the control group, especially in treatment (T7), which gave the best results due to the synergistic effect of Quercetin and Hesperidin in reducing the oxidative effects caused by the pesticide. Concluded of this study that administration by Quercetin and Hesperidin alone or both together and simultaneously with the pesticide has a positive effectiveness and efficiency in reducing the oxidative damage resulting from the toxicity caused by the pesticide in the biochemical parameters, antioxidants and the level of gene expression in male albino rats.

Mağallaẗ Tikrīt li-l-ʻulūm al-ṣirfaẗ, Feb 6, 2023

This study was aimed to isolation and identification of Lactobacillus delubricii subsp. bulgaricu... more This study was aimed to isolation and identification of Lactobacillus delubricii subsp. bulgaricus species and to study the effect of bacterial cells at different degrees of temperature and pH levels or its metabolite products on Inhibition effects against some pathogenic and toxin produced fungi species that's isolated in laboratory. The results was found to identify Lb. delubricii subsp. bulgaricus species were isolated from dairy products and this identification was depended on morphological, cultural and biochemical characters, also were identified four species from fungi were isolated from soil Mycrosporium canis, Trichophyton mentagrophytes , Aspergillus fumigatus and Rhizopus stolinfer. The results also was explored the effects of Lb. delubricii subsp. bulgaricus when cultivated on different pH level and temperature degree and were found the best pH level for inhibition of fungi species growth was at 6.5 and the inhibition zone diameter was 22, 24, 26 and 24 mm respectively, while the 30 C° was get the best inhibition degree for the best inhibition and the inhibition zone diameter against the fungi species was became 22, 24, 26 and 24 mm respectively. The results showed that the best pH levels to and the zone inhibition diameter against the fungi species in this study. The results was also illustrated the effects of the metabolites produced from the Lb. delubricii subsp. bulgaricus at 10 , 20 and 40 mg / ml from cultural media on the Inhibition ability to fungi , and that's appears as significantly increased with the metabolite concentration increased and became at the 40 mg, the inhibition zone diameter was found as 32, 30, 28 and 18mm respectively.

Eurasian Journal of Biosciences, 2020

Objective: The research was aimed to determine the effects of glutathione extracts and some biolo... more Objective: The research was aimed to determine the effects of glutathione extracts and some biological agents on immune and oxidative stress parameters in male rats induced Immunosuppression used Sandimmune. Methods: Glutathione was extract from spinach leaves and estimated the concentration used a high-performance liquid chromatography (HPLC) and determined the effect of orally dosage at one mg/ml alone or with both Zinc or Vit C or cell concentration of Lactobacillus plantarum (CCLP) on the immunity and biological parameters of male rats induced Immunosuppression used Sandimmune and breeding for 28 days. Results: The results indicated that aqueous spinach extract was contained glutathione at a concentration of 246 µg/g in the case of extraction using aqueous extract. The Immunosuppression induced was significantly (p<0.05) decreased of IgA, IgG and IgM values and became at 1022, 2031 and 121.5 mg/dl respectively compared with the control rats group which at 1564, 3206 and 174.7...

Journal of Biology, Agriculture and Healthcare, 2017

This study is aimed at investigating the effect of supplementing 1, 3 and 5% of Sorbitol and Mann... more This study is aimed at investigating the effect of supplementing 1, 3 and 5% of Sorbitol and Mannitol at casing on the productive characteristics of the mushroom Agaricus bisporus . The results revealed a significant increase in the total production and biological efficiency of Agaricus bisporus mushroom .Treatment 1% Sorbitol mushroom recorded the highest production (1286, 90 g/bag) compared with 798.5 g/bag for the control group and 745 g/bag for the Mannitol 1% mushroom (p<0.05).Besides, the biological efficiency was increased from 53.23% to 85.79% with the same supplement. None of the Mannitol supplemented treatment surpassed that of the control group for both traits. However Mannitol 1% recorded significantly higher values when compared with other Mannitol treatments for the total yield and biological efficiency .Sorbitol and Mannitol1% treatments showed significantly the higher values for fruiting body number and the lowest fruit body weight (80, 40 and 16.25 and 17.74, num...

IOP Conference Series: Earth and Environmental Science

The study was conducted in the laboratories of the Department of Food Sciences - College of Agric... more The study was conducted in the laboratories of the Department of Food Sciences - College of Agriculture at the University of Tikrit for the period from the beginning of November 2021 to the end of October 2022 with the aim of testing the efficiency of wrapping soft cheese samples with gelatin film compounded with carboxy methyl cellulose derivatives (CMC) or combined with nanoparticles of zinc oxide. (ZnO-NPs) by determining the microbial counts after storage of samples for 35 days in the refrigerator temperature (4 ºC ± 2). The results showed that cheese samples were contaminated with total microbial counts at 35 days of storage for each T2 and T3 at 1.30, 0.70, CFU/g, respectively, compared with their bacterial count in the control group at 14 days storage, which was at 2.60 CFU/g. The coliform bacteria also appeared in T2 and T3 at Log 0.7 and 0.3 CFU / g, respectively, compared with their counts in T1, at 2.51 CFU / g, while the fungi values of treatment T3, T2 were log 2.3 and ...

Tikrit Journal of Pure Science

This study aims to assay the effect of the separate supplementation with 1, 3 and 5% of (Erbil Fe... more This study aims to assay the effect of the separate supplementation with 1, 3 and 5% of (Erbil Feed, WAFI HOLAND, Lysine, Methionine, Ammonium Chloride and Calcium Nitrate) at casing on the productivity and quality of the mushroom Agaricus bisporus. Results indicated a significant increase in total production and biological efficiency. with treatment of 1% Erbil Feed. When total production increased from 21.68 kg / m2 in the control to 23.20 kg / m2 with this supplement. While the biological efficiency was increased from 72.26% to 77.33% with the same supplement. Treatment 1% of Erbil Feed was also recorded earliest production reached at 16 days after casing than controls at 18 days. The qualitative traits of 3% Methionine has recorded higher of total antioxidants contents at 1975 µg/ g dry weight comparison with control, which was 1572 µg/ g dry weight, as well as recording the 3% of Erbil Feed gave a higher protein contents 36.85% compared to the control treatment 22.36 %.

Euphrates Journal of Agriculture Science, 2019

Tikrit Journal for Agricultural Sciences, 2017

Tikrit Journal for Agricultural Sciences, 2015

Tikrit Journal for Agricultural Sciences, 2013