Kasta Gurning - Academia.edu (original) (raw)
Papers by Kasta Gurning
Jurnal Pendidikan Kimia, Aug 29, 2023
Asian journal of pharmaceutical research and development, Jun 15, 2020
The purpose of this study was to determine at what concentration of ethanol extract of rhizome tu... more The purpose of this study was to determine at what concentration of ethanol extract of rhizome turmeric is active against Escherichia coli, Staphylococcus aureus and Candida albicans. Methods: Ethanolic extract of rhizome turmeric was tested for phytochemical screening by using standard protocol. Antimicrobial testing was using the diffusion disc method to measure the inhibition zone against the Escherichia coli, Staphylococcus aureus and Candida albicans with various concentration of rhizome turmeric extract (500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL). Results: Phytochemical screening showed that ethanolic extract of rhizome turmeric contain alkaloids, flavonoids, saponins, tannins, and triterpenoid/steroid. The antimicrobial inhibition of ethanol extract of rhizome turmeric against Escherichia coli, Staphylococcus aureus and Candida albicans microbia at a concentration of 500 mg/mL had a diameter of 15.88 mm, 15.63 mm and 15.22 mm with a strong category. Conclusions: rhizome turmeric could be use as a novel antimicrobial agent.
Jurnal Pendidikan Kimia, Apr 8, 2023
Rasayan Journal of Chemistry, 2021
Thermostable enzymes (such as amylase) are enzymes with great potential to overcome industrial te... more Thermostable enzymes (such as amylase) are enzymes with great potential to overcome industrial technical problems associated with high-temperature processes. This type of enzyme can be isolated from thermophilic bacteria obtained from hot springs, such as hot springs Rianiate, Samosir. The objectives of this study were to obtain isolates, morphological and biochemical characterization, followed by determining the potential of selected isolates qualitatively. Hot water samples were determined by purposive sampling from two location points, with temperatures 40 °C, 43 °C and 45 °C with a pH of 4. Isolation of thermophilic bacteria using Nutrient Agar (NA) media. There were 12 thermophilic bacterial isolates from two sample locations isolated with the distribution of 2 isolates at 40 °C and 10 isolates at 43 °C, while at 45 °C there were no isolates. The twelve isolates were able to produce an amylase enzyme. The amylase enzyme production was determined by the presence of a clear zone around the colony on the starch agar medium after being dripped with iodine solution. The three isolates that produced the largest clear zone were sp1(2), sp3(1) and sp6, namely 83.1 mm, 79.9 mm and 80.2 mm, respectively. A biochemical test was carried out on isolates with the largest clear zone diameter value. The characterization results showed that the isolate was predicted to belong to the genus Basillus sp. These results indicate that the thermophilic bacteria from the hot springs of Rianiate, Samosir have the potential to produce amylase enzymes.
Asian Journal of Pharmaceutical Research and Development
Objective: This research aims to determine the secondary metabolites contained in the n-hexane ex... more Objective: This research aims to determine the secondary metabolites contained in the n-hexane extract of P. ambionicus leaves and to determine the effective concentration as an antibacterial causing diarrhea. Methods: The research method was carried out experimentally with the stages of making simplicia, maceration, extract making, phytochemical screening, and antibacterial activity testing. Results: The results of phytochemical screening showed the presence of flavonoids, saponins, tannins, steroids/triterpenoids. The results of the antibacterial activity test showed that the n-hexane extract of P.amboinicus leaves at all concentrations (50%, 25%, 12.5%) had antibacterial activity in the strong category (10-20 mm). Conclusion: The n-hexane extract of P.amboinicus leaves has the potential as an antibacterial with a strong category.
Pharmacia
Nangka is a plant that has various kinds of potential both because of the nutritional content pro... more Nangka is a plant that has various kinds of potential both because of the nutritional content provided through the fruit and also part of the content of bioactive compounds contained in the leaves. This study aims to identify the content of bioactive compounds and determine their content using GC-MS and determine the potential antibacterial activity against E. coli, S. aureus, S. epidermis, S. typhi, P. acnes from the ethanol extract of nangka leaves (Artocarpus heterophyllus Lam). Screening results showed positive containing phenolic groups, flavonoids, tannins, saponins, and alkaloids. The results of determining the content of bioactive compounds for phenolics, tannins and flavonoids were 27.654±0.054 mg GAE/g d.w ethanolic extract, 0.46±0.017 mg TAE/g d.w ethanolic extract and 2.978±0.192 mg QE/g d.w ethanolic extract. GC-MS analysis showed the content of octadecanoic acid with a retention time of 36.489 minutes with a concentration of 29.91% and the ethanolic extract of nangka l...
ScienceRise: Pharmaceutical Science
The aim of the study was to isolate and characterize secondary metabolites that have the potentia... more The aim of the study was to isolate and characterize secondary metabolites that have the potential as antioxidants from the ethyl acetate fraction of the leaves of Coleus amboinicus, L. (C. amboinicus). Materials and methods. Purification of the ethyl acetate fraction of C. amboinicus using gravity column chromatography with a stationary phase (silica gel, Merck) and a mobile phase with a solvent ratio of n-hexane (Merck) and ethyl acetate (Merck). Examining isolates includes physical (colour, shape, and melting point). Qualitative purity detection by TLC at 254 nm and 366 nm wavelengths. Structural analysis of metabolites with UV-Vis spectrometer (Spectronic 3000, Genesis 10, Japan), FT-IR(KBr) (Shimadzu IR Prestige-21, Japan), NMR spectrometer (JEOL spectrometer, Japan) operating at 500 MHz (1H-NMR) and 125 MHz (13C-NMR), and Shimadzu's GC-MS (QP-2010S Shimadzu, Japan) and determination of potential antioxidant activity using the DPPH method. Results. The secondary metabolite ...
ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi
The incidence of anemia in pregnant women carries a great risk because the fetus's need for i... more The incidence of anemia in pregnant women carries a great risk because the fetus's need for iron and oxygen supply during development requires iron. This community service activity aims to examine and administer anemia drugs or blood boosters to pregnant women and provide education related to anemia and important nutritional intake for pregnant women to prevent risk factors for anemia. A total of 36 pregnant women patients checked themselves, with 12 in the first trimester, 15 in the second trimester, and 9 in the third trimester. A total of 7 people in the first trimester experienced anemia (19.44%), 10 people in the second trimester (27.77%), and 5 people in the third trimester (13.88%). The rest do not have anemia. This can be due to the fact that the intake of balanced nutrition and foods rich in iron is sufficient during pregnancy. Iron intake during pregnancy in the first trimester is recommended at as much as 0.8 mg/day, while in the second and third trimesters, as much a...
INSOLOGI: Jurnal Sains dan Teknologi
Pseudomonas aeruginosa is a Gram-negative bacterium that can cause nosocomial infections. The use... more Pseudomonas aeruginosa is a Gram-negative bacterium that can cause nosocomial infections. The use of antibiotics in the treatment of non-adherent P. aeruginosa infections increases the risk of antibiotic resistance. Efforts to find new antibiotic compounds from plants continue to be carried out. Papaya leaves are widely spread among the public, which are often used as ingredients for vegetables and have antibacterial potential because they contain papain enzymes and a class of flavonoid compounds. The purpose of this study was to analyze the antibacterial activity of the ethanol extract of papaya leaves (Carica papaya Linn.) against P. aeruginosa by finding the value of the Minimum Inhibitory Concentration (MIC) and calculating the index activity value against the positive control. The method used in this research is Kirby-Bauer (disc diffusion method) with several variations of the test concentration. The results showed that the diameter of the inhibition zone at a concentration of...
![Research paper thumbnail of Formulasi Sediaan Lip Cream Ekstrak Daun Miana (Coleus scuatellariodes [L] Benth.) sebagai Pewarna Alami](https://attachments.academia-assets.com/91803643/thumbnails/1.jpg)
](INSOLOGI: Jurnal Sains dan Teknologi
Miana leaf (Coleus scutellarioides L.) is a plant that contains anthocyanins that can provide cer... more Miana leaf (Coleus scutellarioides L.) is a plant that contains anthocyanins that can provide certain dyes. The purpose of this study was to formulate miana leaf extract in lip cream preparations as a natural dye. The research method includes the manufacture of ethanol extract of miana leaves (Coleus scutellarioides L.) by maceration method. The extract formulas used were 25%, 50%, and 75%. The tests carried out included organoleptic tests, homogeneity tests, physical tests, pH tests, irritation tests and color preference tests. The results of the lip cream preparation, the results of the organoleptic examination were declared to have a semi-solid dosage form, distinctive odor and varied colors F1 pink F2 purplish red, F3 blackish red, homogeneity test results were declared homogeneous, stability test results showed that the preparation did not change shape, color and the odor of the preparation for 14 days of storage, the results of the pH test showed that the preparation was still...
Pharmacia
Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-2 virus. Despite t... more Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-2 virus. Despite the fact that various therapeutic compounds have shown potential prevention or treatment, no specific medicine has been developed for the COVID-19 pandemic. Natural products have recently been suggested as a possible treatment option for COVID-19 prevention and treatment. This study focused on the potential of Coriander sativum L. (CSL) against COVID-19 based on network pharmacology approach. Interested candidates of CSL were identified by searching accessible databases for protein–protein interactions with the COVID-19. An additional GO and KEGG pathway analysis was carried out in order to identify the related mechanism of action. In the end, 51 targets were obtained through network pharmacology analysis with EGFR, AR, JAK2, PARP1, and CTSB become the core target. CSL may have favorable effects on COVID-19 through a number of important pathways, according to GO and KEGG pathway analyses....
Rasayan Journal of Chemistry
The research-based on empirical information from the community that used the leaves of Simargaolg... more The research-based on empirical information from the community that used the leaves of Simargaolgaol (Aglaonema modestum Schott ex Engl.) as traditional treatments. This study aims to determine the class of phytochemical compounds contained in the extracted leaves samples based on the polarity of the solvent. Screening of phytochemical compounds was carried out using standard methods. Toxicity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method for observing the mortality of A. salina Leach larvae and testing for biological activity as antibacterial conducted using the paper disc diffusion method. The study showed that the n-hexane extract had a class of steroid compounds; ethyl acetate extract contains alkaloids, flavonoids, saponins, and steroids while the ethanol extract contains alkaloids, flavonoids, saponins, and tannins. LC50 for n-hexane, ethyl acetate, and ethanol extract was 780.297, 33.083, and 20.548 respectively. The n-hexane and ethyl acetate ex...
Herbal Medicine Journal, Apr 15, 2020
Jurnal Pendidikan Kimia, Aug 29, 2023
Asian journal of pharmaceutical research and development, Jun 15, 2020
The purpose of this study was to determine at what concentration of ethanol extract of rhizome tu... more The purpose of this study was to determine at what concentration of ethanol extract of rhizome turmeric is active against Escherichia coli, Staphylococcus aureus and Candida albicans. Methods: Ethanolic extract of rhizome turmeric was tested for phytochemical screening by using standard protocol. Antimicrobial testing was using the diffusion disc method to measure the inhibition zone against the Escherichia coli, Staphylococcus aureus and Candida albicans with various concentration of rhizome turmeric extract (500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 50 mg/mL, 25 mg/mL). Results: Phytochemical screening showed that ethanolic extract of rhizome turmeric contain alkaloids, flavonoids, saponins, tannins, and triterpenoid/steroid. The antimicrobial inhibition of ethanol extract of rhizome turmeric against Escherichia coli, Staphylococcus aureus and Candida albicans microbia at a concentration of 500 mg/mL had a diameter of 15.88 mm, 15.63 mm and 15.22 mm with a strong category. Conclusions: rhizome turmeric could be use as a novel antimicrobial agent.
Jurnal Pendidikan Kimia, Apr 8, 2023
Rasayan Journal of Chemistry, 2021
Thermostable enzymes (such as amylase) are enzymes with great potential to overcome industrial te... more Thermostable enzymes (such as amylase) are enzymes with great potential to overcome industrial technical problems associated with high-temperature processes. This type of enzyme can be isolated from thermophilic bacteria obtained from hot springs, such as hot springs Rianiate, Samosir. The objectives of this study were to obtain isolates, morphological and biochemical characterization, followed by determining the potential of selected isolates qualitatively. Hot water samples were determined by purposive sampling from two location points, with temperatures 40 °C, 43 °C and 45 °C with a pH of 4. Isolation of thermophilic bacteria using Nutrient Agar (NA) media. There were 12 thermophilic bacterial isolates from two sample locations isolated with the distribution of 2 isolates at 40 °C and 10 isolates at 43 °C, while at 45 °C there were no isolates. The twelve isolates were able to produce an amylase enzyme. The amylase enzyme production was determined by the presence of a clear zone around the colony on the starch agar medium after being dripped with iodine solution. The three isolates that produced the largest clear zone were sp1(2), sp3(1) and sp6, namely 83.1 mm, 79.9 mm and 80.2 mm, respectively. A biochemical test was carried out on isolates with the largest clear zone diameter value. The characterization results showed that the isolate was predicted to belong to the genus Basillus sp. These results indicate that the thermophilic bacteria from the hot springs of Rianiate, Samosir have the potential to produce amylase enzymes.
Asian Journal of Pharmaceutical Research and Development
Objective: This research aims to determine the secondary metabolites contained in the n-hexane ex... more Objective: This research aims to determine the secondary metabolites contained in the n-hexane extract of P. ambionicus leaves and to determine the effective concentration as an antibacterial causing diarrhea. Methods: The research method was carried out experimentally with the stages of making simplicia, maceration, extract making, phytochemical screening, and antibacterial activity testing. Results: The results of phytochemical screening showed the presence of flavonoids, saponins, tannins, steroids/triterpenoids. The results of the antibacterial activity test showed that the n-hexane extract of P.amboinicus leaves at all concentrations (50%, 25%, 12.5%) had antibacterial activity in the strong category (10-20 mm). Conclusion: The n-hexane extract of P.amboinicus leaves has the potential as an antibacterial with a strong category.
Pharmacia
Nangka is a plant that has various kinds of potential both because of the nutritional content pro... more Nangka is a plant that has various kinds of potential both because of the nutritional content provided through the fruit and also part of the content of bioactive compounds contained in the leaves. This study aims to identify the content of bioactive compounds and determine their content using GC-MS and determine the potential antibacterial activity against E. coli, S. aureus, S. epidermis, S. typhi, P. acnes from the ethanol extract of nangka leaves (Artocarpus heterophyllus Lam). Screening results showed positive containing phenolic groups, flavonoids, tannins, saponins, and alkaloids. The results of determining the content of bioactive compounds for phenolics, tannins and flavonoids were 27.654±0.054 mg GAE/g d.w ethanolic extract, 0.46±0.017 mg TAE/g d.w ethanolic extract and 2.978±0.192 mg QE/g d.w ethanolic extract. GC-MS analysis showed the content of octadecanoic acid with a retention time of 36.489 minutes with a concentration of 29.91% and the ethanolic extract of nangka l...
ScienceRise: Pharmaceutical Science
The aim of the study was to isolate and characterize secondary metabolites that have the potentia... more The aim of the study was to isolate and characterize secondary metabolites that have the potential as antioxidants from the ethyl acetate fraction of the leaves of Coleus amboinicus, L. (C. amboinicus). Materials and methods. Purification of the ethyl acetate fraction of C. amboinicus using gravity column chromatography with a stationary phase (silica gel, Merck) and a mobile phase with a solvent ratio of n-hexane (Merck) and ethyl acetate (Merck). Examining isolates includes physical (colour, shape, and melting point). Qualitative purity detection by TLC at 254 nm and 366 nm wavelengths. Structural analysis of metabolites with UV-Vis spectrometer (Spectronic 3000, Genesis 10, Japan), FT-IR(KBr) (Shimadzu IR Prestige-21, Japan), NMR spectrometer (JEOL spectrometer, Japan) operating at 500 MHz (1H-NMR) and 125 MHz (13C-NMR), and Shimadzu's GC-MS (QP-2010S Shimadzu, Japan) and determination of potential antioxidant activity using the DPPH method. Results. The secondary metabolite ...
ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi
The incidence of anemia in pregnant women carries a great risk because the fetus's need for i... more The incidence of anemia in pregnant women carries a great risk because the fetus's need for iron and oxygen supply during development requires iron. This community service activity aims to examine and administer anemia drugs or blood boosters to pregnant women and provide education related to anemia and important nutritional intake for pregnant women to prevent risk factors for anemia. A total of 36 pregnant women patients checked themselves, with 12 in the first trimester, 15 in the second trimester, and 9 in the third trimester. A total of 7 people in the first trimester experienced anemia (19.44%), 10 people in the second trimester (27.77%), and 5 people in the third trimester (13.88%). The rest do not have anemia. This can be due to the fact that the intake of balanced nutrition and foods rich in iron is sufficient during pregnancy. Iron intake during pregnancy in the first trimester is recommended at as much as 0.8 mg/day, while in the second and third trimesters, as much a...
INSOLOGI: Jurnal Sains dan Teknologi
Pseudomonas aeruginosa is a Gram-negative bacterium that can cause nosocomial infections. The use... more Pseudomonas aeruginosa is a Gram-negative bacterium that can cause nosocomial infections. The use of antibiotics in the treatment of non-adherent P. aeruginosa infections increases the risk of antibiotic resistance. Efforts to find new antibiotic compounds from plants continue to be carried out. Papaya leaves are widely spread among the public, which are often used as ingredients for vegetables and have antibacterial potential because they contain papain enzymes and a class of flavonoid compounds. The purpose of this study was to analyze the antibacterial activity of the ethanol extract of papaya leaves (Carica papaya Linn.) against P. aeruginosa by finding the value of the Minimum Inhibitory Concentration (MIC) and calculating the index activity value against the positive control. The method used in this research is Kirby-Bauer (disc diffusion method) with several variations of the test concentration. The results showed that the diameter of the inhibition zone at a concentration of...
INSOLOGI: Jurnal Sains dan Teknologi
Miana leaf (Coleus scutellarioides L.) is a plant that contains anthocyanins that can provide cer... more Miana leaf (Coleus scutellarioides L.) is a plant that contains anthocyanins that can provide certain dyes. The purpose of this study was to formulate miana leaf extract in lip cream preparations as a natural dye. The research method includes the manufacture of ethanol extract of miana leaves (Coleus scutellarioides L.) by maceration method. The extract formulas used were 25%, 50%, and 75%. The tests carried out included organoleptic tests, homogeneity tests, physical tests, pH tests, irritation tests and color preference tests. The results of the lip cream preparation, the results of the organoleptic examination were declared to have a semi-solid dosage form, distinctive odor and varied colors F1 pink F2 purplish red, F3 blackish red, homogeneity test results were declared homogeneous, stability test results showed that the preparation did not change shape, color and the odor of the preparation for 14 days of storage, the results of the pH test showed that the preparation was still...
Pharmacia
Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-2 virus. Despite t... more Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-2 virus. Despite the fact that various therapeutic compounds have shown potential prevention or treatment, no specific medicine has been developed for the COVID-19 pandemic. Natural products have recently been suggested as a possible treatment option for COVID-19 prevention and treatment. This study focused on the potential of Coriander sativum L. (CSL) against COVID-19 based on network pharmacology approach. Interested candidates of CSL were identified by searching accessible databases for protein–protein interactions with the COVID-19. An additional GO and KEGG pathway analysis was carried out in order to identify the related mechanism of action. In the end, 51 targets were obtained through network pharmacology analysis with EGFR, AR, JAK2, PARP1, and CTSB become the core target. CSL may have favorable effects on COVID-19 through a number of important pathways, according to GO and KEGG pathway analyses....
Rasayan Journal of Chemistry
The research-based on empirical information from the community that used the leaves of Simargaolg... more The research-based on empirical information from the community that used the leaves of Simargaolgaol (Aglaonema modestum Schott ex Engl.) as traditional treatments. This study aims to determine the class of phytochemical compounds contained in the extracted leaves samples based on the polarity of the solvent. Screening of phytochemical compounds was carried out using standard methods. Toxicity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method for observing the mortality of A. salina Leach larvae and testing for biological activity as antibacterial conducted using the paper disc diffusion method. The study showed that the n-hexane extract had a class of steroid compounds; ethyl acetate extract contains alkaloids, flavonoids, saponins, and steroids while the ethanol extract contains alkaloids, flavonoids, saponins, and tannins. LC50 for n-hexane, ethyl acetate, and ethanol extract was 780.297, 33.083, and 20.548 respectively. The n-hexane and ethyl acetate ex...
Herbal Medicine Journal, Apr 15, 2020