Katarina Vitošević - Academia.edu (original) (raw)

Papers by Katarina Vitošević

Legal Medicine, Dec 31, 2023

Forensic Science, Medicine and Pathology, Jan 2, 2024

Experimental and Applied Biomedical Research (EABR), Aug 31, 2023

Journal of Medical Virology, Sep 30, 2023

Vojnosanitetski pregled

Background/Aim. Traffic accident injuries are a major public health problem worldwide, with milli... more Background/Aim. Traffic accident injuries are a major public health problem worldwide, with millions of people dying every year. Although the improvement of traffic safety is based on preventive action, it is necessary to conduct an analysis of victims in traffic in order to prevent future traffic accidents with the help of such findings. The aim of the study was to determine the manner of occurrence of abdominal injuries (AIs), gender differences, and the sitting position in the vehicle that affects the severity of AIs, as well as which AIs lead more often to fatalities in traffic accidents. Methods. Materials for the present study were collected from the medico-legal autopsies, showing AIs that occurred in road traffic accidents. The total number of people who died from sustained injuries or complications of the injuries after road traffic accidents was 525. Results. In the study sample, 38.3% of subjects sustained abdominal trauma. Men were more likely to have AIs, and passengers...

Romanian Journal of Legal Medicine, Jun 10, 2021

Molecular Biology Reports

Praxis medica, 2015

Тokom oružanog sukoba na Kosovu i Metohiji, i posle njega, poginulo je više od 5 000 osoba, a pre... more Тokom oružanog sukoba na Kosovu i Metohiji, i posle njega, poginulo je više od 5 000 osoba, a prema popisu Međunarodnog Komiteta Crvenog Krsta iz marta 2001. godine, 3.338 osoba su se još vodile kao nestale. Do zaključivanja ovog rada, broj nestalih lica na prostoru Kosova i Metohije za kojima se traga iznosi oko 1700. Ovaj spisak uključuje ljude iz svih etničkih zajednica.. Primarni cilj forenzičke istrage je utvrditi identitet nestalog lica i nakon toga njegove ostatke predati porodici u skladu sa Ženevskim protokolima. Postmortalnim pregledom nakon izvršene ekshumacije stručnjaci utvrđuju i uzrok i način smrti koji ukazuje na kršenje ljudskih prava i na počinjene zločine. Zaključak. Razložan je strah da će sa protokom vremena sve manje interesovanja biti posvećeno ovoj temi i da će aktivnosti na pronalasku i identifikaciji nestalih potpuno zamreti.

Background: There are enormous formalin fixed paraffin embedded tissue archives and constantly gr... more Background: There are enormous formalin fixed paraffin embedded tissue archives and constantly growing number of methods for molecular analyses but, isolation of DNA from this tissue is still challenging due to the damage effect of formalin on DNA. To determine the extent to which DNA purity, quantity and integrity depends on the process of fixation in formalin, and to what extent on the process of tissue paraffin embedding, we compared the quality of DNA isolated from fixed tissues and DNA isolated from tissues embedded in paraffin blocks after fixation. Methods and Results: Heart, liver and brain tissues obtained from healthy people who suddenly died a violent death were fixed in 10% buffered formalin as well as in 4% unbuffered formalin 6h, 1-7 days (every 24h), 10, 14, 28 days and 2 months. Also the same tissues were fixed in 4% unbuffered formalin and embedded in paraffin block and stored from few months to 30 years. Yield and purity of the DNA samples isolated from these tissu...

Serbian Journal of Experimental and Clinical Research, 2019

Formalin fixation is a widely used method in histopathology that has certain limits. Formalin oft... more Formalin fixation is a widely used method in histopathology that has certain limits. Formalin often leads to the degradation of DNA molecules in cancer tissues, which makes tissues unusable for molecular analysis. The other factors may also affect the quality of DNA isolated from fixed tissues. The aim of this study is to determine the impact of the incubation time and temperature on the quality of DNA molecules isolated from various healthy human tissues. The brain, lung and kidney tissues, excluded during the forensic autopsies of people who died of violent death, were fixed in phosphate-buffered formalin from 24h to two months. After the completion of the incubation period, the DNA was isolated using phenol-chloroform-isoamyl alcohol extraction method and the concentration and purity of the samples were determined spectrophotometrically. The degree of degradation of DNA was assessed by PCR reaction, by amplification of gene fragments which lengths were 150bp (GPD1) and 262bp (β-a...

Iranian Journal of Public Health

The Article is not available.

Vojnosanitetski pregled, 2019

Background / Aim. In road traffic accidents, chest injuries are a critical factor since death usu... more Background / Aim. In road traffic accidents, chest injuries are a critical factor since death usually occurs as a result of injuries to the heart and lungs, flail chest, pneumothorax, exsanguination etc. The aim of this study was to analyse the most vulnerable subjects in road accidents, as well as the most frequent and most significant types of chest injuries sustained by different categories of subjects, and to examine the relevance of certain injuries or risk factors to outliving the injuries. Methods. The autopsy, retrospective and cross-sectional study was performed at the Clinical Centre of Kragujevac, Department of Forensic Medicine and Toxicology. The study included all the participants in road traffic accidents on the territory of Kragujevac and the surrounding area who died from the injuries sustained or due to complications of the injuries during the period from 2001 to 2016. The subjects were divided into the following groups: pedestrians, motor vehicle drivers, front-seat passengers, back-seat passengers, bicyclists, motorcyclists and tractor drivers. Occurrence of injuries in three regions of the body (chest, head and abdomen) was analysed in all the subjects. Results. The study included 525 subjects who died due to injuries sustained in traffic accidents, which makes up for 38.4% of the total number of 1,366 autopsy cases covered by the study period. The average age of the subjects was 52±19 years. The study sample consisted of 391 (74.5%) men and 134 (25.5%) women. The most vulnerable subjects were pedestrians (220, i.e. 41.9%), followed by motor vehicle drivers (98, i.e. 18.7%), front-seat passengers (79, i.e. 15%), motorcyclists (39, i.e. 7.4%), bicyclists (38, i.e. 7.2%), back-seat passengers (29, i.e. 5.5%) and tractor drivers (22, i.e. 4.2%). Chest injuries were identified in 408 subjects (77.7%), while the most frequent type of injury was rib fracture, observed in two thirds of the sample. Out of the total number, 291 (55.4%) subjects died at the scene of the accident or on their way to the hospital, while 234 (44.6%) of them outlived injuries for a certain period of time. Drivers exhibited the highest risk of dying at the scene, while bicyclists outlived their injuries more frequently. Conclusion. Chest injuries are very common in subjects who died at the scene of road traffic accident.

Serbian Journal of Experimental and Clinical Research, 2019

Forensic genetics represents a combination of molecular and population genetics. Personal identif... more Forensic genetics represents a combination of molecular and population genetics. Personal identification and kinship analysis (e.g. paternity testing) are the two main subjects of forensic DNA analysis. Biological specimens from which DNA is isolated are blood, semen, saliva, tissues, bones, teeth, hairs. Genotyping has become a basis in the characterization of forensic biological evidence. It is performed using a variety of genetic markers, which are divided into two large groups: bi-allelic (single-nucleotide polymorphisms, SNP) and multi-allelic polymorphisms (variable number of tandem repeats, VNTR and short tandem repeats, STR). This review describes the purpose of genetic markers in forensic investigation and their limitations. The STR loci are currently the most informative genetic markers for identity testing, but in cases without a suspect SNP can predict offender’s ancestry and phenotype traits such as skin, eyes and hair color. Nowadays, many countries worldwide have esta...

Medicinski podmladak

Introduction: Fixation with formalin, a widely used procedure to preserve tissue samples, leads t... more Introduction: Fixation with formalin, a widely used procedure to preserve tissue samples, leads to damage of DNA through crosslinking activity. The factors that could influence the fixation and integrity of DNA may be numerous: incubation time, tissue type, concentration of formalin, temperature, pH and viscosity. Aim: The aim of this investigation was to examine the influence of incubation time and temperature of formalin fixation on the yield, purity and integrity of DNA isolated from healthy human heart myocardial tissue taken during medico-legal autopsy. Material and methods: Heart tissue samples were fixed in phosphate-buffered formalin at +4°C in the dark, as well as at room temperature in the presence of light. The DNA was isolated after one day, then successively every day during the first week, and then on the tenth day, and after two and four-week periods using phenol-chloroform-isoamyl alcohol extraction method. The absorbances were measured at 260 nm and 280 nm, which allows calculation of yield and purity of nucleic acid in the samples. The PCR amplification of two genes, GPDH (150 bp) and ß-actin (262 bp), were performed to evaluate the degree of DNA molecule fragmentation. Results: The highest yield, purity and preserved integrity of DNA were obtained from the samples fixed in formalin at +4°C in the darkness. In these samples, GPDH and ß-actin genes were amplified up to 14 days, unlike the samples that were fixed at the room temperature in which the ß-actin gene was amplified up to 5 days, while the GPDH gene fragment was successfully amplified up to 10 days of fixation. Conclusion: The temperature, presence of light and the incubation time of formalin fixation all have important influences on yield, purity and integrity of DNA during the fixation process.

Forensic Science, Medicine and Pathology

Acta histochemica, Jan 14, 2018

The aim of this study is to investigate the effects of formalin fixation on the degradation of DN... more The aim of this study is to investigate the effects of formalin fixation on the degradation of DNA molecules in five different healthy tissues exempted during the autopsy, as well as the selection of the method that is most suitable for the DNA isolation. Heart muscle, liver, brain, lung and kidney tissue obtained from the healthy people who suddenly died from a violent death were used. The parts of tissue were fixed in 10% phosphate-buffered formalin as well as in 4% unbuffered formalin at room temperature. Morphology of tissue was studied using H&E staining. The DNA was isolated 6 h, 1-7 days (every 24 h), 10, 14, 28 days and 2 months after fixation using two different methods: extraction with phenol-chloroform-isoamyl alcohol as well as with PureLink Genomic DNA Kit. Yield and purity of the DNA samples were measured spectrophotometrically at 260 nm and 280 nm. The PCR amplifications of the glycerol-3-phosphate dehydrogenase 1 (GPD1, 150 bp), ß actin (ACTB, 262 bp) and ribosomal p...

Legal Medicine, Dec 31, 2023

Forensic Science, Medicine and Pathology, Jan 2, 2024

Experimental and Applied Biomedical Research (EABR), Aug 31, 2023

Journal of Medical Virology, Sep 30, 2023

Vojnosanitetski pregled

Background/Aim. Traffic accident injuries are a major public health problem worldwide, with milli... more Background/Aim. Traffic accident injuries are a major public health problem worldwide, with millions of people dying every year. Although the improvement of traffic safety is based on preventive action, it is necessary to conduct an analysis of victims in traffic in order to prevent future traffic accidents with the help of such findings. The aim of the study was to determine the manner of occurrence of abdominal injuries (AIs), gender differences, and the sitting position in the vehicle that affects the severity of AIs, as well as which AIs lead more often to fatalities in traffic accidents. Methods. Materials for the present study were collected from the medico-legal autopsies, showing AIs that occurred in road traffic accidents. The total number of people who died from sustained injuries or complications of the injuries after road traffic accidents was 525. Results. In the study sample, 38.3% of subjects sustained abdominal trauma. Men were more likely to have AIs, and passengers...

Romanian Journal of Legal Medicine, Jun 10, 2021

Molecular Biology Reports

Praxis medica, 2015

Тokom oružanog sukoba na Kosovu i Metohiji, i posle njega, poginulo je više od 5 000 osoba, a pre... more Тokom oružanog sukoba na Kosovu i Metohiji, i posle njega, poginulo je više od 5 000 osoba, a prema popisu Međunarodnog Komiteta Crvenog Krsta iz marta 2001. godine, 3.338 osoba su se još vodile kao nestale. Do zaključivanja ovog rada, broj nestalih lica na prostoru Kosova i Metohije za kojima se traga iznosi oko 1700. Ovaj spisak uključuje ljude iz svih etničkih zajednica.. Primarni cilj forenzičke istrage je utvrditi identitet nestalog lica i nakon toga njegove ostatke predati porodici u skladu sa Ženevskim protokolima. Postmortalnim pregledom nakon izvršene ekshumacije stručnjaci utvrđuju i uzrok i način smrti koji ukazuje na kršenje ljudskih prava i na počinjene zločine. Zaključak. Razložan je strah da će sa protokom vremena sve manje interesovanja biti posvećeno ovoj temi i da će aktivnosti na pronalasku i identifikaciji nestalih potpuno zamreti.

Background: There are enormous formalin fixed paraffin embedded tissue archives and constantly gr... more Background: There are enormous formalin fixed paraffin embedded tissue archives and constantly growing number of methods for molecular analyses but, isolation of DNA from this tissue is still challenging due to the damage effect of formalin on DNA. To determine the extent to which DNA purity, quantity and integrity depends on the process of fixation in formalin, and to what extent on the process of tissue paraffin embedding, we compared the quality of DNA isolated from fixed tissues and DNA isolated from tissues embedded in paraffin blocks after fixation. Methods and Results: Heart, liver and brain tissues obtained from healthy people who suddenly died a violent death were fixed in 10% buffered formalin as well as in 4% unbuffered formalin 6h, 1-7 days (every 24h), 10, 14, 28 days and 2 months. Also the same tissues were fixed in 4% unbuffered formalin and embedded in paraffin block and stored from few months to 30 years. Yield and purity of the DNA samples isolated from these tissu...

Serbian Journal of Experimental and Clinical Research, 2019

Formalin fixation is a widely used method in histopathology that has certain limits. Formalin oft... more Formalin fixation is a widely used method in histopathology that has certain limits. Formalin often leads to the degradation of DNA molecules in cancer tissues, which makes tissues unusable for molecular analysis. The other factors may also affect the quality of DNA isolated from fixed tissues. The aim of this study is to determine the impact of the incubation time and temperature on the quality of DNA molecules isolated from various healthy human tissues. The brain, lung and kidney tissues, excluded during the forensic autopsies of people who died of violent death, were fixed in phosphate-buffered formalin from 24h to two months. After the completion of the incubation period, the DNA was isolated using phenol-chloroform-isoamyl alcohol extraction method and the concentration and purity of the samples were determined spectrophotometrically. The degree of degradation of DNA was assessed by PCR reaction, by amplification of gene fragments which lengths were 150bp (GPD1) and 262bp (β-a...

Iranian Journal of Public Health

The Article is not available.

Vojnosanitetski pregled, 2019

Background / Aim. In road traffic accidents, chest injuries are a critical factor since death usu... more Background / Aim. In road traffic accidents, chest injuries are a critical factor since death usually occurs as a result of injuries to the heart and lungs, flail chest, pneumothorax, exsanguination etc. The aim of this study was to analyse the most vulnerable subjects in road accidents, as well as the most frequent and most significant types of chest injuries sustained by different categories of subjects, and to examine the relevance of certain injuries or risk factors to outliving the injuries. Methods. The autopsy, retrospective and cross-sectional study was performed at the Clinical Centre of Kragujevac, Department of Forensic Medicine and Toxicology. The study included all the participants in road traffic accidents on the territory of Kragujevac and the surrounding area who died from the injuries sustained or due to complications of the injuries during the period from 2001 to 2016. The subjects were divided into the following groups: pedestrians, motor vehicle drivers, front-seat passengers, back-seat passengers, bicyclists, motorcyclists and tractor drivers. Occurrence of injuries in three regions of the body (chest, head and abdomen) was analysed in all the subjects. Results. The study included 525 subjects who died due to injuries sustained in traffic accidents, which makes up for 38.4% of the total number of 1,366 autopsy cases covered by the study period. The average age of the subjects was 52±19 years. The study sample consisted of 391 (74.5%) men and 134 (25.5%) women. The most vulnerable subjects were pedestrians (220, i.e. 41.9%), followed by motor vehicle drivers (98, i.e. 18.7%), front-seat passengers (79, i.e. 15%), motorcyclists (39, i.e. 7.4%), bicyclists (38, i.e. 7.2%), back-seat passengers (29, i.e. 5.5%) and tractor drivers (22, i.e. 4.2%). Chest injuries were identified in 408 subjects (77.7%), while the most frequent type of injury was rib fracture, observed in two thirds of the sample. Out of the total number, 291 (55.4%) subjects died at the scene of the accident or on their way to the hospital, while 234 (44.6%) of them outlived injuries for a certain period of time. Drivers exhibited the highest risk of dying at the scene, while bicyclists outlived their injuries more frequently. Conclusion. Chest injuries are very common in subjects who died at the scene of road traffic accident.

Serbian Journal of Experimental and Clinical Research, 2019

Forensic genetics represents a combination of molecular and population genetics. Personal identif... more Forensic genetics represents a combination of molecular and population genetics. Personal identification and kinship analysis (e.g. paternity testing) are the two main subjects of forensic DNA analysis. Biological specimens from which DNA is isolated are blood, semen, saliva, tissues, bones, teeth, hairs. Genotyping has become a basis in the characterization of forensic biological evidence. It is performed using a variety of genetic markers, which are divided into two large groups: bi-allelic (single-nucleotide polymorphisms, SNP) and multi-allelic polymorphisms (variable number of tandem repeats, VNTR and short tandem repeats, STR). This review describes the purpose of genetic markers in forensic investigation and their limitations. The STR loci are currently the most informative genetic markers for identity testing, but in cases without a suspect SNP can predict offender’s ancestry and phenotype traits such as skin, eyes and hair color. Nowadays, many countries worldwide have esta...

Medicinski podmladak

Introduction: Fixation with formalin, a widely used procedure to preserve tissue samples, leads t... more Introduction: Fixation with formalin, a widely used procedure to preserve tissue samples, leads to damage of DNA through crosslinking activity. The factors that could influence the fixation and integrity of DNA may be numerous: incubation time, tissue type, concentration of formalin, temperature, pH and viscosity. Aim: The aim of this investigation was to examine the influence of incubation time and temperature of formalin fixation on the yield, purity and integrity of DNA isolated from healthy human heart myocardial tissue taken during medico-legal autopsy. Material and methods: Heart tissue samples were fixed in phosphate-buffered formalin at +4°C in the dark, as well as at room temperature in the presence of light. The DNA was isolated after one day, then successively every day during the first week, and then on the tenth day, and after two and four-week periods using phenol-chloroform-isoamyl alcohol extraction method. The absorbances were measured at 260 nm and 280 nm, which allows calculation of yield and purity of nucleic acid in the samples. The PCR amplification of two genes, GPDH (150 bp) and ß-actin (262 bp), were performed to evaluate the degree of DNA molecule fragmentation. Results: The highest yield, purity and preserved integrity of DNA were obtained from the samples fixed in formalin at +4°C in the darkness. In these samples, GPDH and ß-actin genes were amplified up to 14 days, unlike the samples that were fixed at the room temperature in which the ß-actin gene was amplified up to 5 days, while the GPDH gene fragment was successfully amplified up to 10 days of fixation. Conclusion: The temperature, presence of light and the incubation time of formalin fixation all have important influences on yield, purity and integrity of DNA during the fixation process.

Forensic Science, Medicine and Pathology

Acta histochemica, Jan 14, 2018

The aim of this study is to investigate the effects of formalin fixation on the degradation of DN... more The aim of this study is to investigate the effects of formalin fixation on the degradation of DNA molecules in five different healthy tissues exempted during the autopsy, as well as the selection of the method that is most suitable for the DNA isolation. Heart muscle, liver, brain, lung and kidney tissue obtained from the healthy people who suddenly died from a violent death were used. The parts of tissue were fixed in 10% phosphate-buffered formalin as well as in 4% unbuffered formalin at room temperature. Morphology of tissue was studied using H&E staining. The DNA was isolated 6 h, 1-7 days (every 24 h), 10, 14, 28 days and 2 months after fixation using two different methods: extraction with phenol-chloroform-isoamyl alcohol as well as with PureLink Genomic DNA Kit. Yield and purity of the DNA samples were measured spectrophotometrically at 260 nm and 280 nm. The PCR amplifications of the glycerol-3-phosphate dehydrogenase 1 (GPD1, 150 bp), ß actin (ACTB, 262 bp) and ribosomal p...