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Papers by Katherine Launier

Research paper thumbnail of The state of science on including inhalation within supervised consumption services: A scoping review of academic and grey literature

International Journal of Drug Policy

Research paper thumbnail of Matrix metalloproteinase-2 mediate oncostatin-M induced cardiomyocyte dedifferentiation (1151.2)

Research paper thumbnail of Canadian Indigenous Women’s Perspectives of Maternal Health and Health Care Services: A Systematic Review

Development of policies and interventions to address health disparities between Indigenous and no... more Development of policies and interventions to address health disparities between Indigenous and non-Indigenous populations requires a comprehensive understanding of Indigenous people’s experiences and perspectives of healthcare services. We systematically reviewed the published literature on Canadian Indigenous women’s experiences and perspectives of maternal healthcare during pregnancy, childbirth, and the postpartum period. Major bibliographic databases (including PubMed, CINAHL, EMBASE, SCOPUS, and SSCI) were searched for published studies (1990–March 2015) in English. Reference lists of identified articles were searched to identify additional articles. 92 articles were retrieved for further review, of which 16 studies were included: 8 on maternal healthcare and/or medical evacuation; 3 on gestational diabetes mellitus; 3 on the impact of policies on maternal health; and 2 on maternal weight changes and/or breastfeeding. The included studies described 1043 participants: Indigenous...

Research paper thumbnail of To what extent do supervised drug consumption services incorporate non-injection routes of administration? A systematic scoping review documenting existing facilities

Harm Reduction Journal

Background Most of the existing research on supervised consumption services (SCS) is focused on i... more Background Most of the existing research on supervised consumption services (SCS) is focused on injection drug use. Less is known about the applicability of SCS for people who consume drugs orally, intranasally, or through inhalation. This is problematic because people who use drugs through modes other than injection are also at risk of overdose death and other harm, and experience barriers accessing health and social services. We aimed to describe existing SCS models that accommodate these alternate routes of drug consumption, and synthesize available information on characteristics of program participants. Methods We conducted a systematic scoping review of 9 peer-reviewed and 13 grey literature databases on SCS that incorporate non-injection routes of consumption. We screened 22,882 titles, and excluded 22,843 (99.8%) articles. We ultimately included 39 (0.2%) full-text articles; 28 (72%) of these articles explicitly identified SCS that permit alternate routes of consumption and 2...

Research paper thumbnail of Canadian Indigenous Womens Perspectives of Maternal Health and Health Care Services: A Systematic Review

Diversity & Equality in Health and Care

Research paper thumbnail of Matrix metalloproteinase-2 in oncostatin M-induced sarcomere degeneration in cardiomyocytes

American journal of physiology. Heart and circulatory physiology, Jul 6, 2016

Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facili... more Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facilitating cardiac repair. Cardiomyocyte dedifferentiation and proliferation induced by oncostatin-M (OSM) is characterized by sarcomere degeneration. However, the mechanism underlying sarcomere degeneration remains unclear. We hypothesized that this process may involve matrix metalloproteinase-2 (MMP-2), a key protease localized at the sarcomere in cardiomyocytes. To test the hypothesis that MMP-2 is involved in the sarcomere degeneration that characterizes cardiomyocyte dedifferentiation. Confocal immunofluorescence and biochemical methods were used to explore the role of MMP-2 in OSM-induced dedifferentiation of neonatal rat ventricular myocytes (NRVM). OSM caused a concentration- and time-dependent loss of sarcomeric α-actinin and troponin-I in NRVM. Upon OSM-treatment, the mature sarcomere transformed to a phenotype resembling a less developed sarcomere, i.e., loss of sarcomeric protein...

Research paper thumbnail of Matrix metalloproteinase-2 mediate oncostatin-M induced cardiomyocyte dedifferentiation (1151.2)

The Faseb Journal, Apr 1, 2014

Research paper thumbnail of Matrix metalloproteinase-2 in oncostatin M-induced sarcomere degeneration in cardiomyocytes

Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facili... more Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facilitating cardiac repair. Cardiomyocyte dedifferentiation and proliferation induced by oncostatin-M (OSM) is characterized by sarcomere degeneration. However, the mechanism underlying sarcomere degeneration remains unclear. We hypothesized that this process may involve matrix metalloproteinase-2 (MMP-2), a key protease localized at the sarcomere in cardiomyocytes. We tested the hypothesis that MMP-2 is involved in the sarcomere degeneration that characterizes cardiomyocyte dedifferentiation. Confocal immunofluorescence and biochemical methods were used to explore the role of MMP-2 in OSM-induced dedifferentiation of neonatal rat ventricular myocytes (NRVM). OSM caused a concentration-and time-dependent loss of sarcomeric ␣-actinin and troponin-I in NRVM. Upon OSM-treatment, the mature sarcomere transformed to a phenotype resembling a lessdeveloped sarcomere, i.e., loss of sarcomeric proteins and Z-disk transformed into disconnected Z bodies, characteristic of immature myofibrils. OSM dose dependently increased MMP-2 activity. Both the pan-MMP inhibitor GM6001 and the selective MMP-2 inhibitor ARP 100 prevented sarcomere degeneration induced by OSM treatment. OSM also induced NRVM cell cycling and increased methylthiazolyl-tetrazolium (MTT) staining, preventable by MMP inhibition. These results suggest that MMP-2 mediates sarcomere degeneration in OSM-induced cardiomyocyte dedifferentiation and thus potentially contributes to cardiomyocyte regeneration. oncostatin-M; cardiomyocytes; sarcomere; dedifferentiation; matrix metalloproteinase-2 NEW & NOTEWORTHY The cytokine oncostatin-M (OSM) can induce cardiomyocyte dedifferentiation, associated with sarcomere degeneration. We find that OSM treatment of cardiomyocytes increases matrix metalloproteinase-2 (MMP-2) expression and activity and that pharmaceutical inhibition of MMP-2 prevents OSM-induced sarcomere degeneration. This suggests a previously unknown role for MMP-2 in modulation of sarcomere structure and integrity.

Research paper thumbnail of The state of science on including inhalation within supervised consumption services: A scoping review of academic and grey literature

International Journal of Drug Policy

Research paper thumbnail of Matrix metalloproteinase-2 mediate oncostatin-M induced cardiomyocyte dedifferentiation (1151.2)

Research paper thumbnail of Canadian Indigenous Women’s Perspectives of Maternal Health and Health Care Services: A Systematic Review

Development of policies and interventions to address health disparities between Indigenous and no... more Development of policies and interventions to address health disparities between Indigenous and non-Indigenous populations requires a comprehensive understanding of Indigenous people’s experiences and perspectives of healthcare services. We systematically reviewed the published literature on Canadian Indigenous women’s experiences and perspectives of maternal healthcare during pregnancy, childbirth, and the postpartum period. Major bibliographic databases (including PubMed, CINAHL, EMBASE, SCOPUS, and SSCI) were searched for published studies (1990–March 2015) in English. Reference lists of identified articles were searched to identify additional articles. 92 articles were retrieved for further review, of which 16 studies were included: 8 on maternal healthcare and/or medical evacuation; 3 on gestational diabetes mellitus; 3 on the impact of policies on maternal health; and 2 on maternal weight changes and/or breastfeeding. The included studies described 1043 participants: Indigenous...

Research paper thumbnail of To what extent do supervised drug consumption services incorporate non-injection routes of administration? A systematic scoping review documenting existing facilities

Harm Reduction Journal

Background Most of the existing research on supervised consumption services (SCS) is focused on i... more Background Most of the existing research on supervised consumption services (SCS) is focused on injection drug use. Less is known about the applicability of SCS for people who consume drugs orally, intranasally, or through inhalation. This is problematic because people who use drugs through modes other than injection are also at risk of overdose death and other harm, and experience barriers accessing health and social services. We aimed to describe existing SCS models that accommodate these alternate routes of drug consumption, and synthesize available information on characteristics of program participants. Methods We conducted a systematic scoping review of 9 peer-reviewed and 13 grey literature databases on SCS that incorporate non-injection routes of consumption. We screened 22,882 titles, and excluded 22,843 (99.8%) articles. We ultimately included 39 (0.2%) full-text articles; 28 (72%) of these articles explicitly identified SCS that permit alternate routes of consumption and 2...

Research paper thumbnail of Canadian Indigenous Womens Perspectives of Maternal Health and Health Care Services: A Systematic Review

Diversity & Equality in Health and Care

Research paper thumbnail of Matrix metalloproteinase-2 in oncostatin M-induced sarcomere degeneration in cardiomyocytes

American journal of physiology. Heart and circulatory physiology, Jul 6, 2016

Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facili... more Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facilitating cardiac repair. Cardiomyocyte dedifferentiation and proliferation induced by oncostatin-M (OSM) is characterized by sarcomere degeneration. However, the mechanism underlying sarcomere degeneration remains unclear. We hypothesized that this process may involve matrix metalloproteinase-2 (MMP-2), a key protease localized at the sarcomere in cardiomyocytes. To test the hypothesis that MMP-2 is involved in the sarcomere degeneration that characterizes cardiomyocyte dedifferentiation. Confocal immunofluorescence and biochemical methods were used to explore the role of MMP-2 in OSM-induced dedifferentiation of neonatal rat ventricular myocytes (NRVM). OSM caused a concentration- and time-dependent loss of sarcomeric α-actinin and troponin-I in NRVM. Upon OSM-treatment, the mature sarcomere transformed to a phenotype resembling a less developed sarcomere, i.e., loss of sarcomeric protein...

Research paper thumbnail of Matrix metalloproteinase-2 mediate oncostatin-M induced cardiomyocyte dedifferentiation (1151.2)

The Faseb Journal, Apr 1, 2014

Research paper thumbnail of Matrix metalloproteinase-2 in oncostatin M-induced sarcomere degeneration in cardiomyocytes

Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facili... more Cardiomyocyte dedifferentiation may be an important source of proliferating cardiomyocytes facilitating cardiac repair. Cardiomyocyte dedifferentiation and proliferation induced by oncostatin-M (OSM) is characterized by sarcomere degeneration. However, the mechanism underlying sarcomere degeneration remains unclear. We hypothesized that this process may involve matrix metalloproteinase-2 (MMP-2), a key protease localized at the sarcomere in cardiomyocytes. We tested the hypothesis that MMP-2 is involved in the sarcomere degeneration that characterizes cardiomyocyte dedifferentiation. Confocal immunofluorescence and biochemical methods were used to explore the role of MMP-2 in OSM-induced dedifferentiation of neonatal rat ventricular myocytes (NRVM). OSM caused a concentration-and time-dependent loss of sarcomeric ␣-actinin and troponin-I in NRVM. Upon OSM-treatment, the mature sarcomere transformed to a phenotype resembling a lessdeveloped sarcomere, i.e., loss of sarcomeric proteins and Z-disk transformed into disconnected Z bodies, characteristic of immature myofibrils. OSM dose dependently increased MMP-2 activity. Both the pan-MMP inhibitor GM6001 and the selective MMP-2 inhibitor ARP 100 prevented sarcomere degeneration induced by OSM treatment. OSM also induced NRVM cell cycling and increased methylthiazolyl-tetrazolium (MTT) staining, preventable by MMP inhibition. These results suggest that MMP-2 mediates sarcomere degeneration in OSM-induced cardiomyocyte dedifferentiation and thus potentially contributes to cardiomyocyte regeneration. oncostatin-M; cardiomyocytes; sarcomere; dedifferentiation; matrix metalloproteinase-2 NEW & NOTEWORTHY The cytokine oncostatin-M (OSM) can induce cardiomyocyte dedifferentiation, associated with sarcomere degeneration. We find that OSM treatment of cardiomyocytes increases matrix metalloproteinase-2 (MMP-2) expression and activity and that pharmaceutical inhibition of MMP-2 prevents OSM-induced sarcomere degeneration. This suggests a previously unknown role for MMP-2 in modulation of sarcomere structure and integrity.