Kazuhiko Tanaka - Academia.edu (original) (raw)
Papers by Kazuhiko Tanaka
Xenobiotica; the fate of foreign compounds in biological systems, Jan 12, 2017
1. Drug-induced liver injury is difficult to predict at the pre-clinical stage. This study aimed ... more 1. Drug-induced liver injury is difficult to predict at the pre-clinical stage. This study aimed to clarify the roles of caspase-8 and -9 in CYP2E1 metabolite-induced liver injury in both rats and cell cultures in vitro treated with carbon tetrachloride (CCl4), halothane or sevoflurane. The human hepatocarcinoma functional liver cell line was maintained in 3-dimensional culture alone or in co-culture with human acute monocytic leukemia cells. 2. In vivo, laboratory indices of liver dysfunction and histology were normal after administration of sevoflurane. CCl4 treatment increased blood AST/ALT levels, liver caspase-3 and -9 activities and liver malondialdehyde, accompanied by centrilobular hepatocyte necrosis. Halothane increased AST/ALT levels, caspase-3 and -8 activities (but not malondialdehyde) concomitant with widespread hepatotoxicity. In vitro, CCl4 treatment increased caspase-9 activity and decreased both mitochondrial membrane potential (MMP) and cell viability. In co-cultu...
Journal of Chromatography a, May 17, 2002
Se Pu Chinese Journal of Chromatography Zhongguo Hua Xue Hui, Apr 1, 2012
A combination of hydrophilic interaction chromatographic (HILIC) column and a weakly acidic catio... more A combination of hydrophilic interaction chromatographic (HILIC) column and a weakly acidic cation-exchange resin (WCX) column was used for simultaneous separation of inorganic anions and cations by ion chromatography (IC). Firstly, the capability of HILIC column for the separation of analyte ions was evaluated under acidic eluent conditions. The columns used were SeQuant ZIC-HILIC (ZIC-HILIC) with a sulfobetaine-zwitterion stationary phase (ZIC-HILIC) and Acclaim HILIC-10 with a diol stationary phase (HILIC-10). When using tartaric acid as the eluent, the HILIC columns indicated strong retentions for anions, based on ion-pair interaction. Especially, HILIC-10 could strongly retain anions compared with ZIC-HILIC. The selectivity for analyte anions of HILIC-10 with 5 mmol/L tartaric acid eluent was in the order of I(-) > NO3(-) > Br(-) > Cl(-) > H2PO4(-). However, since HILIC-10 could not separate analyte cations, a WCX column (TSKgel Super IC-A/C) was connected after the HILIC column in series. The combination column system of HILIC and WCX columns could successfully separate ten ions (Na+, NH4+, K+, Mg2+, Ca2+, H2PO4(-), Cl(-), Br(-), NO3(-) and I(-)) with elution of 4 mmol/L tartaric acid plus 8 mmol/L 18-crown-6. The relative standard deviations (RSDs) of analyte ions by the system were in the ranges of 0.02% - 0.05% in retention times and 0.18% - 5.3% in peak areas through three-time successive injections. The limits of detection at signal-to-noise ratio of 3 were 0.24 - 0.30 micromol/L for the cations and 0.31 - 1.2 micromol/L for the anions. This system was applied for the simultaneous determination of the cations and the anions in a vegetable juice sample with satisfactory results.
Fresenius J Anal Chem, 2000
European Journal of Pharmacology, Jun 22, 2007
Transplantation Proceedings, Jul 1, 1996
Cancer Research, May 1, 1982
Journal of Chromatography a, May 16, 2003
A new and simple approach is described for the determination of the haloacetic acids (such as mon... more A new and simple approach is described for the determination of the haloacetic acids (such as mono-, di- and trichloroacetic acids) usually found in drinking water as chlorination by-products after disinfection processes and acetic acid. The new approach, termed vacancy ion-exclusion chromatography, is based on an ion-exclusion mechanism but using the sample solution as the mobile phase, pure water as the injected sample, and a weakly acidic cation-exchange resin column (TSKgel OApak-A) as the stationary phase. The addition of sulfuric acid to the mobile phase results in highly sensitive conductivity detection with sharp and well-shaped peaks, leading to excellent and efficient separations. The elution order was sulfuric acid, dichloroacetic acid, monochloroacetic acid, trichloroacetic acid, and acetic acid. The separation of these acids depends on their pKa values. Acids with lower pKa values were eluted earlier than those with higher pKa, except for trichloroacetic acid due to a hydrophobic-adsorption effect occurring as a side-effect of vacancy ion-exclusion chromatography. The detection limits of these acids in the present study with conductivity detection were 3.4 microM for monochloroacetic acid, 0.86 microM for dichloroacetic acid and 0.15 microM for trichloroacetic acid.
Eur J Pediatr Surg, 1992
A very rare case of mosaic type trisomy 18 associated with hepatoblastoma is described. The patie... more A very rare case of mosaic type trisomy 18 associated with hepatoblastoma is described. The patient underwent an extended right hepatic lobectomy at 2 years of age, and the resected tumor was diagnosed as a fetal type dominant hepatoblastoma. The results of chromosome analysis demonstrated that in the peripheral blood and skin, the trisomy 18 cells were 80% and 67%, respectively. On the other hand, although virtually 100% of the cells in the normal liver tissue were 46, XX, about one third of the cells were trisomy 18 in the tumor tissue. At 2 years and 9 months after the operation, the patient was generally healthy and had no evidence of recurrence.
Analytica Chimica Acta, Nov 1, 1998
Transplantation Proceedings, Jul 1, 1996
Journal of Chromatography a, Jul 30, 1999
A new type of zwitterionic surfactant, N-{2-[acetyl(3-sulfopropyl)amino]ethyl}-N,N-dimethyldodeca... more A new type of zwitterionic surfactant, N-{2-[acetyl(3-sulfopropyl)amino]ethyl}-N,N-dimethyldodecanaminium hydroxide (ammonium sulfobetaine-1), with a greater distance between the two charged groups, was used as the stationary phase for electrostatic ion chromatography (EIC) of polarizable anions (e.g., thiocyanate, iodide and nitrate) in saline water samples. The targeted species (polarizable anions) were baseline separated using this type of zwitterionic surfactant as the stationary phase, but the highly polarizable species (iodide and thiocyanate) were eluted faster (compared with the results obtained using N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, C12N3S, with a shorter distance between the two charged groups, as the stationary phase). In other words, the extent of binding of the highly polarizable anion (iodide and thiocyanate) was found to be smaller when using ammonium sulfobetaine-1 as the stationary phase. This provides a rapid but effective method for the analysis of highly polarizable anions in saline water samples. The results for the successful detection of iodide in seawater demonstrates the usefulness of this new type of zwitterionic surfactants for EIC.
Anal Sci, 2001
A unified ion chromatographic (IC) system was developed for the determination of acidity or alkal... more A unified ion chromatographic (IC) system was developed for the determination of acidity or alkalinity. Separation column used was a reversed-phase ODS packed column, which had been modified by saturating it with lithium dodecylsulfate. A slightly acidified LiCl (50 mM LiCl and 0.05 mM H2SO4) aqueous solution was used as the eluent. By conditioning the separation column in this way, both H+ and Li+ ions became bound to the stationary phase. Dodecylsulfate groups with Li+ counterions acted as cation-exchange sites for the separation of hydrogen ions (free acidity determination). The remaining dodecylsulfate groups, with H+ counterions acted as a titrant, which reacted with basic species (total alkalinity determination). The acidity or alkalinity of each sample was measured according to the change in conductance from the eluent baseline level. A positive peak was observed from those samples with a free acidity greater than their total alkalinity, due to the separation/elution of free H+ ions. A negative peak was observed from those samples with a free acidity less than their total alkalinity. This was due to an equivalent amount of eluent H+ ions being re-supplied to the stationary phase while the "solid titrant" consumed by the acid-base reaction was regenerated. The retention time for the peak corresponding to the acidity or alkalinity was governed by the retention time for H+ ions in this IC system. Samples with a free acidity greater than 2.25 microM (tested by determination of H+ ions in pure water in equilibrium with atmospheric CO2) could be analyzed by this method. A very similar detection level was obtained for alkalinity (tested by analyzing standard aqueous NaHCO3 solutions). Aqueous solutions of some strong-acid/strong-base inorganic salts were found to be slightly alkaline. This was measured as a percentage, relative to an NaHCO3 solution at the same concentration. Solutions of NaClO4, Na2SO4, NaI, NaNO3, and NaCl, gave comparative alkalinity values of 8.75%, 1.83%, 0.42%, 0.35%, and 0.33%, respectively.
Thrombosis Research, Apr 15, 1986
The thrombolytic effect of single-chain pro-urokinase (SCPU) was examined in the rabbit using a j... more The thrombolytic effect of single-chain pro-urokinase (SCPU) was examined in the rabbit using a jugular vein thrombosis model. Infusion of a low dose (120,000 IU/kg) of either urokinase (UK) or SCPU did not produce any significant thrombolysis. However, UK administration at such a low dose caused 20% degradation of circulating fibrinogen. A high dose (480,000 IU/kg) caused significant thrombolysis. The degree of fibrinogenolysis was about 20% in SCPU, but about 80% in UK. The thrombolytic efficiency of SCPU was thus about 3 times larger than that of UK. Analysis of fibrinolytic parameters such as plasminogen, alpha 2-plasmin inhibitor, etc. suggested that UK caused systemic activation of the fibrinolytic system, but SCPU, locally limited activation on the fibrin surface (fibrinolysis). These results indicate that SCPU represents a highly efficient thrombolytic agent without producing fibrinogenolysis.
Http Dx Doi Org 10 1081 Jlc 100107723, Jun 1, 2007
ABSTRACT Weakly acidic cation exchange resin can be used for simultaneous separation of anions an... more ABSTRACT Weakly acidic cation exchange resin can be used for simultaneous separation of anions and cations when acidic eluents were employed. On the weakly acidic cation exchange resin, anions were retained by ion exclusion mechanism, while cations by cation exchange mechanism. The selection of eluent is very important for the separation of interesting analytes. In this study, a mixture of 0.6 mmol/L 2,6-pyridinedicarboxylic acid (PDCA), 0.75 mmol/L sulfosalicylic acid (SSA), and 6.0 mmol/L 18-crown-6 was employed as eluent for the simultaneous separation of common inorganic anions (Cl, NO3 and SO4 ) and cations (Na, NH4 , K, Mg, and Ca).A good separation of these anions and cations on the weakly acidic cation exchange column (Tosoh TSKgel OApak-A) was achieved in 16 min. The linear range of peak area calibration curves for all analytes were up to two orders of magnitude. The conductivity detection limits calculated at S/N=3 were from 0.2 to 1.6 μmol/L for anions and cations. The method developed in this work was successfully applied to the simultaneous determination of major inorganic anions and cations in several environmental water samples.
Journal of Chromatography a, May 16, 2003
Xenobiotica; the fate of foreign compounds in biological systems, Jan 12, 2017
1. Drug-induced liver injury is difficult to predict at the pre-clinical stage. This study aimed ... more 1. Drug-induced liver injury is difficult to predict at the pre-clinical stage. This study aimed to clarify the roles of caspase-8 and -9 in CYP2E1 metabolite-induced liver injury in both rats and cell cultures in vitro treated with carbon tetrachloride (CCl4), halothane or sevoflurane. The human hepatocarcinoma functional liver cell line was maintained in 3-dimensional culture alone or in co-culture with human acute monocytic leukemia cells. 2. In vivo, laboratory indices of liver dysfunction and histology were normal after administration of sevoflurane. CCl4 treatment increased blood AST/ALT levels, liver caspase-3 and -9 activities and liver malondialdehyde, accompanied by centrilobular hepatocyte necrosis. Halothane increased AST/ALT levels, caspase-3 and -8 activities (but not malondialdehyde) concomitant with widespread hepatotoxicity. In vitro, CCl4 treatment increased caspase-9 activity and decreased both mitochondrial membrane potential (MMP) and cell viability. In co-cultu...
Journal of Chromatography a, May 17, 2002
Se Pu Chinese Journal of Chromatography Zhongguo Hua Xue Hui, Apr 1, 2012
A combination of hydrophilic interaction chromatographic (HILIC) column and a weakly acidic catio... more A combination of hydrophilic interaction chromatographic (HILIC) column and a weakly acidic cation-exchange resin (WCX) column was used for simultaneous separation of inorganic anions and cations by ion chromatography (IC). Firstly, the capability of HILIC column for the separation of analyte ions was evaluated under acidic eluent conditions. The columns used were SeQuant ZIC-HILIC (ZIC-HILIC) with a sulfobetaine-zwitterion stationary phase (ZIC-HILIC) and Acclaim HILIC-10 with a diol stationary phase (HILIC-10). When using tartaric acid as the eluent, the HILIC columns indicated strong retentions for anions, based on ion-pair interaction. Especially, HILIC-10 could strongly retain anions compared with ZIC-HILIC. The selectivity for analyte anions of HILIC-10 with 5 mmol/L tartaric acid eluent was in the order of I(-) > NO3(-) > Br(-) > Cl(-) > H2PO4(-). However, since HILIC-10 could not separate analyte cations, a WCX column (TSKgel Super IC-A/C) was connected after the HILIC column in series. The combination column system of HILIC and WCX columns could successfully separate ten ions (Na+, NH4+, K+, Mg2+, Ca2+, H2PO4(-), Cl(-), Br(-), NO3(-) and I(-)) with elution of 4 mmol/L tartaric acid plus 8 mmol/L 18-crown-6. The relative standard deviations (RSDs) of analyte ions by the system were in the ranges of 0.02% - 0.05% in retention times and 0.18% - 5.3% in peak areas through three-time successive injections. The limits of detection at signal-to-noise ratio of 3 were 0.24 - 0.30 micromol/L for the cations and 0.31 - 1.2 micromol/L for the anions. This system was applied for the simultaneous determination of the cations and the anions in a vegetable juice sample with satisfactory results.
Fresenius J Anal Chem, 2000
European Journal of Pharmacology, Jun 22, 2007
Transplantation Proceedings, Jul 1, 1996
Cancer Research, May 1, 1982
Journal of Chromatography a, May 16, 2003
A new and simple approach is described for the determination of the haloacetic acids (such as mon... more A new and simple approach is described for the determination of the haloacetic acids (such as mono-, di- and trichloroacetic acids) usually found in drinking water as chlorination by-products after disinfection processes and acetic acid. The new approach, termed vacancy ion-exclusion chromatography, is based on an ion-exclusion mechanism but using the sample solution as the mobile phase, pure water as the injected sample, and a weakly acidic cation-exchange resin column (TSKgel OApak-A) as the stationary phase. The addition of sulfuric acid to the mobile phase results in highly sensitive conductivity detection with sharp and well-shaped peaks, leading to excellent and efficient separations. The elution order was sulfuric acid, dichloroacetic acid, monochloroacetic acid, trichloroacetic acid, and acetic acid. The separation of these acids depends on their pKa values. Acids with lower pKa values were eluted earlier than those with higher pKa, except for trichloroacetic acid due to a hydrophobic-adsorption effect occurring as a side-effect of vacancy ion-exclusion chromatography. The detection limits of these acids in the present study with conductivity detection were 3.4 microM for monochloroacetic acid, 0.86 microM for dichloroacetic acid and 0.15 microM for trichloroacetic acid.
Eur J Pediatr Surg, 1992
A very rare case of mosaic type trisomy 18 associated with hepatoblastoma is described. The patie... more A very rare case of mosaic type trisomy 18 associated with hepatoblastoma is described. The patient underwent an extended right hepatic lobectomy at 2 years of age, and the resected tumor was diagnosed as a fetal type dominant hepatoblastoma. The results of chromosome analysis demonstrated that in the peripheral blood and skin, the trisomy 18 cells were 80% and 67%, respectively. On the other hand, although virtually 100% of the cells in the normal liver tissue were 46, XX, about one third of the cells were trisomy 18 in the tumor tissue. At 2 years and 9 months after the operation, the patient was generally healthy and had no evidence of recurrence.
Analytica Chimica Acta, Nov 1, 1998
Transplantation Proceedings, Jul 1, 1996
Journal of Chromatography a, Jul 30, 1999
A new type of zwitterionic surfactant, N-{2-[acetyl(3-sulfopropyl)amino]ethyl}-N,N-dimethyldodeca... more A new type of zwitterionic surfactant, N-{2-[acetyl(3-sulfopropyl)amino]ethyl}-N,N-dimethyldodecanaminium hydroxide (ammonium sulfobetaine-1), with a greater distance between the two charged groups, was used as the stationary phase for electrostatic ion chromatography (EIC) of polarizable anions (e.g., thiocyanate, iodide and nitrate) in saline water samples. The targeted species (polarizable anions) were baseline separated using this type of zwitterionic surfactant as the stationary phase, but the highly polarizable species (iodide and thiocyanate) were eluted faster (compared with the results obtained using N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, C12N3S, with a shorter distance between the two charged groups, as the stationary phase). In other words, the extent of binding of the highly polarizable anion (iodide and thiocyanate) was found to be smaller when using ammonium sulfobetaine-1 as the stationary phase. This provides a rapid but effective method for the analysis of highly polarizable anions in saline water samples. The results for the successful detection of iodide in seawater demonstrates the usefulness of this new type of zwitterionic surfactants for EIC.
Anal Sci, 2001
A unified ion chromatographic (IC) system was developed for the determination of acidity or alkal... more A unified ion chromatographic (IC) system was developed for the determination of acidity or alkalinity. Separation column used was a reversed-phase ODS packed column, which had been modified by saturating it with lithium dodecylsulfate. A slightly acidified LiCl (50 mM LiCl and 0.05 mM H2SO4) aqueous solution was used as the eluent. By conditioning the separation column in this way, both H+ and Li+ ions became bound to the stationary phase. Dodecylsulfate groups with Li+ counterions acted as cation-exchange sites for the separation of hydrogen ions (free acidity determination). The remaining dodecylsulfate groups, with H+ counterions acted as a titrant, which reacted with basic species (total alkalinity determination). The acidity or alkalinity of each sample was measured according to the change in conductance from the eluent baseline level. A positive peak was observed from those samples with a free acidity greater than their total alkalinity, due to the separation/elution of free H+ ions. A negative peak was observed from those samples with a free acidity less than their total alkalinity. This was due to an equivalent amount of eluent H+ ions being re-supplied to the stationary phase while the "solid titrant" consumed by the acid-base reaction was regenerated. The retention time for the peak corresponding to the acidity or alkalinity was governed by the retention time for H+ ions in this IC system. Samples with a free acidity greater than 2.25 microM (tested by determination of H+ ions in pure water in equilibrium with atmospheric CO2) could be analyzed by this method. A very similar detection level was obtained for alkalinity (tested by analyzing standard aqueous NaHCO3 solutions). Aqueous solutions of some strong-acid/strong-base inorganic salts were found to be slightly alkaline. This was measured as a percentage, relative to an NaHCO3 solution at the same concentration. Solutions of NaClO4, Na2SO4, NaI, NaNO3, and NaCl, gave comparative alkalinity values of 8.75%, 1.83%, 0.42%, 0.35%, and 0.33%, respectively.
Thrombosis Research, Apr 15, 1986
The thrombolytic effect of single-chain pro-urokinase (SCPU) was examined in the rabbit using a j... more The thrombolytic effect of single-chain pro-urokinase (SCPU) was examined in the rabbit using a jugular vein thrombosis model. Infusion of a low dose (120,000 IU/kg) of either urokinase (UK) or SCPU did not produce any significant thrombolysis. However, UK administration at such a low dose caused 20% degradation of circulating fibrinogen. A high dose (480,000 IU/kg) caused significant thrombolysis. The degree of fibrinogenolysis was about 20% in SCPU, but about 80% in UK. The thrombolytic efficiency of SCPU was thus about 3 times larger than that of UK. Analysis of fibrinolytic parameters such as plasminogen, alpha 2-plasmin inhibitor, etc. suggested that UK caused systemic activation of the fibrinolytic system, but SCPU, locally limited activation on the fibrin surface (fibrinolysis). These results indicate that SCPU represents a highly efficient thrombolytic agent without producing fibrinogenolysis.
Http Dx Doi Org 10 1081 Jlc 100107723, Jun 1, 2007
ABSTRACT Weakly acidic cation exchange resin can be used for simultaneous separation of anions an... more ABSTRACT Weakly acidic cation exchange resin can be used for simultaneous separation of anions and cations when acidic eluents were employed. On the weakly acidic cation exchange resin, anions were retained by ion exclusion mechanism, while cations by cation exchange mechanism. The selection of eluent is very important for the separation of interesting analytes. In this study, a mixture of 0.6 mmol/L 2,6-pyridinedicarboxylic acid (PDCA), 0.75 mmol/L sulfosalicylic acid (SSA), and 6.0 mmol/L 18-crown-6 was employed as eluent for the simultaneous separation of common inorganic anions (Cl, NO3 and SO4 ) and cations (Na, NH4 , K, Mg, and Ca).A good separation of these anions and cations on the weakly acidic cation exchange column (Tosoh TSKgel OApak-A) was achieved in 16 min. The linear range of peak area calibration curves for all analytes were up to two orders of magnitude. The conductivity detection limits calculated at S/N=3 were from 0.2 to 1.6 μmol/L for anions and cations. The method developed in this work was successfully applied to the simultaneous determination of major inorganic anions and cations in several environmental water samples.
Journal of Chromatography a, May 16, 2003