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Papers by Nicole Kelly

Research paper thumbnail of Photobleaching Kinetics of Optically Trapped Multilamellar Vesicles Containing Verteporfin Using Two-photon Excitation

Photochemistry and Photobiology, 2006

TPE-PDT has advantages over one-photon PDT, including lower collateral damage to healthy tissue a... more TPE-PDT has advantages over one-photon PDT, including lower collateral damage to healthy tissue and more precise delivery of PDT. As with one-photon PDT, there can be local photochemical depletion of oxygen during TPE-PDT. Here, we investigate model systems and live cells to measure local photosensitizer photobleaching and through it, infer local oxygen consumption in therapeutic volumes of the order 1 pm3. Multilamellar vesicles (MLV) and African green monkey kidney ((3-1) cells were used to study the TPE photobleaching dynamics of the photosensitizer, Verteporfin. It was found that in an oxygen-rich environment, photobleaching kinetics could not be modeled using a mono-exponential function, whereas in hypoxic conditions a mono-exponential decay was adequate to represent photobleaching. A biexponential was found to adequately model the oxygen-rich conditions and it is hypothesized that the fast part of the decay is oxygendependent, whereas the slower rate constant is largely oxygen-independent. Photobleaching recovery studies in the CV-1 cells support this hypothesis.

Research paper thumbnail of Photobleaching Kinetics of Optically Trapped Multilamellar Vesicles Containing Verteporfin Using Two-photon Excitation

Photochemistry and Photobiology, 2006

TPE-PDT has advantages over one-photon PDT, including lower collateral damage to healthy tissue a... more TPE-PDT has advantages over one-photon PDT, including lower collateral damage to healthy tissue and more precise delivery of PDT. As with one-photon PDT, there can be local photochemical depletion of oxygen during TPE-PDT. Here, we investigate model systems and live cells to measure local photosensitizer photobleaching and through it, infer local oxygen consumption in therapeutic volumes of the order 1 pm3. Multilamellar vesicles (MLV) and African green monkey kidney ((3-1) cells were used to study the TPE photobleaching dynamics of the photosensitizer, Verteporfin. It was found that in an oxygen-rich environment, photobleaching kinetics could not be modeled using a mono-exponential function, whereas in hypoxic conditions a mono-exponential decay was adequate to represent photobleaching. A biexponential was found to adequately model the oxygen-rich conditions and it is hypothesized that the fast part of the decay is oxygendependent, whereas the slower rate constant is largely oxygen-independent. Photobleaching recovery studies in the CV-1 cells support this hypothesis.

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