Kenneth Storey - Academia.edu (original) (raw)

Papers by Kenneth Storey

Research paper thumbnail of Regulation of coenzyme utilization by bovine liver glutamate dehydrogenase: investigations using thionicotinamide analogues of NAD and NADP in a dual wavelength assay

The International journal of biochemistry, 1982

1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wa... more 1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wavelength spectroscopy and pairing the thionicotinamide analogues, S-NAD or S-NADP (which have absorbance maxima at 400 nm), with the natural coenzymes, NADP or NAD. 2. S-NAD and S-NADP were found to be good alternate substrates for GDH: the apparent Km's for the thioderivatives were similar to those of the corresponding natural coenzymes, the apparent Km's for glutamate were unaltered by the substitution of the thioderivatives, and the effects of inhibitors and activators on S-NAD or S-NADP kinetics were qualitatively the same as those found for NAD or NADP, respectively. 3. Dual wavelength assays paired NAD and S-NADP or S-NAD and NADP to study the simultaneous reduction of the two coenzymes. Conditions of increasing glutamate concentrations produced differential effects on the rates of the NAD vs NADP reactions, the result, with either nucleotide pair, promoting the NADP linked...

Research paper thumbnail of Glycolytic enzymes in muscle of the pacific dolphin: role of pyruvate kinase in aerobic-anaerobic transition during diving

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1974

Research paper thumbnail of Freeze tolerance and intolerance as strategies of winter survival in terrestrially-hibernating amphibians

Comparative biochemistry and physiology. A, Comparative physiology, 1986

The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in... more The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in seven species of terrestrially-hibernating amphibians found in eastern Canada. All species had only moderate supercooling abilities, with whole animal supercooling points of -1.5 to -3 degrees C. Two salamander species, Plethodon cinereus and Ambystoma laterale, and the toad, Bufo americanus, were freezing intolerant and were killed when frozen for 24 hr at temperatures just below their supercooling points. The major winter strategy of these animals appears to behavioural avoidance of subzero temperatures. Four species of frogs Rana sylvatica, Hyla versicolor, Hyla crucifer and Pseudacris triseriata, survived extracellular freezing at moderate subzero temperatures (-2 to -4 degrees C) for periods of time ranging up to 2 weeks. All four frog species accumulated low molecular weight carbohydrates as cryoprotectants, glycerol being the major cryoprotectant in adult H. versicolor, while imm...

Research paper thumbnail of Design of acetylcholinesterase for its physical environment

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Pyruvate kinase from oplophorus Gracilirostris muscle

Comparative biochemistry and physiology. B, Comparative biochemistry, 1976

Research paper thumbnail of Hydrogen shuttles in air versus water breathing fishes

Comparative biochemistry and physiology. B, Comparative biochemistry, 1979

1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaim... more 1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaima, Lepidosiren, and Synbranchus (obligate air breathers), Hoplerythriunus (facultative air breather), and Osteoglossum and Hoplias (obligate water breathers). 2. Although no respiratory evidence for significant alpha-glycerophosphate cycle participation could be shown in the air breathers, this cycle was demonstrable in hearts of water breathers. 3. In agreement with the O2 uptake studies, it was possible to reconstruct the malate-aspartate, but not the alpha-glycerophosphate cycle, in isolated mitochondria from air breathers, while both shuttles could be reconstructed with heart mitochondria in the case of water breathing fishes.

Research paper thumbnail of Hydrogen shuttles in gills of water versus air breathing osteoglossids

Comparative biochemistry and physiology. B, Comparative biochemistry, 1979

1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a... more 1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a related osteoglossid that is an obligate water breather, a comparison was made of the relative roles of the malate-aspartate cycle and the alpha-glycerophosphate (alpha-GP) cycle in transferring reducing equivalents from the cytosol to the mitochondria. 2. In aruana gill preparations, the alpha-GP cycle could be most clearly demonstrated by reconstructing it with purified isolated mitochondria, using the oxidation rate of exogenous NADH as a measure of the cycling activity. 3. Subcellular preparations of Arapaima gill, in contrast to the aruana gill, were not responsive to exogenous alpha-glycerophosphate, but a glutamate-malate stimulated O2 uptake was sensitive to aminooxyacetate, an aminotransferase inhibitor, a result that would be expected if the respiration were based on malate-aspartate cycling. 4. It was concluded that, compared to the alpha-glycerophosphate cycle, the malate-as...

Research paper thumbnail of Gill citrate synthase from an abyssal fish

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Squid muscle malic enzyme

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Squid muscle glyceraldehyde-3-phosphate dehydrogenase: control of the enzyme in a tissue with an active alpha-glycero-P cycle

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of The kinetic requirements of cytoplasmic alpha-glycerophosphate (alpha-GP) dehydrogenase in muscles with active alpha-GP cycles

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Alpha-glycerophosphate dehydrogenase: its role in the control of the cytoplasmic arm of the alpha-glycerophosphate cycle in squid mantle

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Squid muscle fructose diphosphatase and its role in the control of F6P-FDP cycling

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Redox regulation of muscle phosphofructokinase in a fast swimming squid

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Physiology, Biochemistry, and Molecular Biology of Vertebrate Freeze Tolerance

Life in the Frozen State, 2004

Research paper thumbnail of Expression profiling and structural characterization of microRNAs in adipose tissues of hibernating ground squirrels

Genomics, proteomics & bioinformatics, 2014

MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. I... more MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. In this study, we determined the expression and structural characteristics of 20 miRNAs in brown (BAT) and white adipose tissue (WAT) during torpor in thirteen-lined ground squirrels. Using a modified stem-loop technique, we found that during torpor, expression of six miRNAs including let-7a, let-7b, miR-107, miR-150, miR-222 and miR-31 was significantly downregulated in WAT (P<0.05), which was 16%-54% of euthermic non-torpid control squirrels, whereas expression of three miRNAs including miR-143, miR-200a and miR-519d was found to be upregulated by 1.32-2.34-fold. Similarly, expression of more miRNAs was downregulated in BAT during torpor. We detected reduced expression of 6 miRNAs including miR-103a, miR-107, miR-125b, miR-21, miR-221 and miR-31 (48%-70% of control), while only expression of miR-138 was significantly upregulated (2.91±0.8-fold of the control, P<0.05). Interestingl...

Research paper thumbnail of Substrate specificities of octopine dehydrogenases from marine invertebrates

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1982

Research paper thumbnail of Sodium chromate demonstrates some insulin-mimetic properties in the fruit fly Drosophila melanogaster

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2015

Research paper thumbnail of Intracellular distribution of enzymes associated with lipogenesis and gluconeogenesis in fat body of the adult cockroach, Periplaneta

Insect Biochemistry, 1978

Research paper thumbnail of The intracellular distribution of enzymes of carbohydrate degradation in the fat body of the adult male cockroach

Insect Biochemistry, 1978

Research paper thumbnail of Regulation of coenzyme utilization by bovine liver glutamate dehydrogenase: investigations using thionicotinamide analogues of NAD and NADP in a dual wavelength assay

The International journal of biochemistry, 1982

1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wa... more 1. The coenzyme preference of bovine liver glutamate dehydrogenase (GDH) was probed using dual wavelength spectroscopy and pairing the thionicotinamide analogues, S-NAD or S-NADP (which have absorbance maxima at 400 nm), with the natural coenzymes, NADP or NAD. 2. S-NAD and S-NADP were found to be good alternate substrates for GDH: the apparent Km's for the thioderivatives were similar to those of the corresponding natural coenzymes, the apparent Km's for glutamate were unaltered by the substitution of the thioderivatives, and the effects of inhibitors and activators on S-NAD or S-NADP kinetics were qualitatively the same as those found for NAD or NADP, respectively. 3. Dual wavelength assays paired NAD and S-NADP or S-NAD and NADP to study the simultaneous reduction of the two coenzymes. Conditions of increasing glutamate concentrations produced differential effects on the rates of the NAD vs NADP reactions, the result, with either nucleotide pair, promoting the NADP linked...

Research paper thumbnail of Glycolytic enzymes in muscle of the pacific dolphin: role of pyruvate kinase in aerobic-anaerobic transition during diving

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1974

Research paper thumbnail of Freeze tolerance and intolerance as strategies of winter survival in terrestrially-hibernating amphibians

Comparative biochemistry and physiology. A, Comparative physiology, 1986

The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in... more The ability to tolerate extracellular freezing as an adaptation for winter survival was tested in seven species of terrestrially-hibernating amphibians found in eastern Canada. All species had only moderate supercooling abilities, with whole animal supercooling points of -1.5 to -3 degrees C. Two salamander species, Plethodon cinereus and Ambystoma laterale, and the toad, Bufo americanus, were freezing intolerant and were killed when frozen for 24 hr at temperatures just below their supercooling points. The major winter strategy of these animals appears to behavioural avoidance of subzero temperatures. Four species of frogs Rana sylvatica, Hyla versicolor, Hyla crucifer and Pseudacris triseriata, survived extracellular freezing at moderate subzero temperatures (-2 to -4 degrees C) for periods of time ranging up to 2 weeks. All four frog species accumulated low molecular weight carbohydrates as cryoprotectants, glycerol being the major cryoprotectant in adult H. versicolor, while imm...

Research paper thumbnail of Design of acetylcholinesterase for its physical environment

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Pyruvate kinase from oplophorus Gracilirostris muscle

Comparative biochemistry and physiology. B, Comparative biochemistry, 1976

Research paper thumbnail of Hydrogen shuttles in air versus water breathing fishes

Comparative biochemistry and physiology. B, Comparative biochemistry, 1979

1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaim... more 1. The malate-aspartate cycle was demonstrable in subcellular preparations of hearts from Arapaima, Lepidosiren, and Synbranchus (obligate air breathers), Hoplerythriunus (facultative air breather), and Osteoglossum and Hoplias (obligate water breathers). 2. Although no respiratory evidence for significant alpha-glycerophosphate cycle participation could be shown in the air breathers, this cycle was demonstrable in hearts of water breathers. 3. In agreement with the O2 uptake studies, it was possible to reconstruct the malate-aspartate, but not the alpha-glycerophosphate cycle, in isolated mitochondria from air breathers, while both shuttles could be reconstructed with heart mitochondria in the case of water breathing fishes.

Research paper thumbnail of Hydrogen shuttles in gills of water versus air breathing osteoglossids

Comparative biochemistry and physiology. B, Comparative biochemistry, 1979

1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a... more 1. Using subcellular preparations of gills from Arapaima, an obligate air breather, and aruana, a related osteoglossid that is an obligate water breather, a comparison was made of the relative roles of the malate-aspartate cycle and the alpha-glycerophosphate (alpha-GP) cycle in transferring reducing equivalents from the cytosol to the mitochondria. 2. In aruana gill preparations, the alpha-GP cycle could be most clearly demonstrated by reconstructing it with purified isolated mitochondria, using the oxidation rate of exogenous NADH as a measure of the cycling activity. 3. Subcellular preparations of Arapaima gill, in contrast to the aruana gill, were not responsive to exogenous alpha-glycerophosphate, but a glutamate-malate stimulated O2 uptake was sensitive to aminooxyacetate, an aminotransferase inhibitor, a result that would be expected if the respiration were based on malate-aspartate cycling. 4. It was concluded that, compared to the alpha-glycerophosphate cycle, the malate-as...

Research paper thumbnail of Gill citrate synthase from an abyssal fish

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Squid muscle malic enzyme

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Squid muscle glyceraldehyde-3-phosphate dehydrogenase: control of the enzyme in a tissue with an active alpha-glycero-P cycle

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of The kinetic requirements of cytoplasmic alpha-glycerophosphate (alpha-GP) dehydrogenase in muscles with active alpha-GP cycles

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Alpha-glycerophosphate dehydrogenase: its role in the control of the cytoplasmic arm of the alpha-glycerophosphate cycle in squid mantle

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Squid muscle fructose diphosphatase and its role in the control of F6P-FDP cycling

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Redox regulation of muscle phosphofructokinase in a fast swimming squid

Comparative biochemistry and physiology. B, Comparative biochemistry, Jan 15, 1975

Research paper thumbnail of Physiology, Biochemistry, and Molecular Biology of Vertebrate Freeze Tolerance

Life in the Frozen State, 2004

Research paper thumbnail of Expression profiling and structural characterization of microRNAs in adipose tissues of hibernating ground squirrels

Genomics, proteomics & bioinformatics, 2014

MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. I... more MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. In this study, we determined the expression and structural characteristics of 20 miRNAs in brown (BAT) and white adipose tissue (WAT) during torpor in thirteen-lined ground squirrels. Using a modified stem-loop technique, we found that during torpor, expression of six miRNAs including let-7a, let-7b, miR-107, miR-150, miR-222 and miR-31 was significantly downregulated in WAT (P<0.05), which was 16%-54% of euthermic non-torpid control squirrels, whereas expression of three miRNAs including miR-143, miR-200a and miR-519d was found to be upregulated by 1.32-2.34-fold. Similarly, expression of more miRNAs was downregulated in BAT during torpor. We detected reduced expression of 6 miRNAs including miR-103a, miR-107, miR-125b, miR-21, miR-221 and miR-31 (48%-70% of control), while only expression of miR-138 was significantly upregulated (2.91±0.8-fold of the control, P<0.05). Interestingl...

Research paper thumbnail of Substrate specificities of octopine dehydrogenases from marine invertebrates

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1982

Research paper thumbnail of Sodium chromate demonstrates some insulin-mimetic properties in the fruit fly Drosophila melanogaster

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2015

Research paper thumbnail of Intracellular distribution of enzymes associated with lipogenesis and gluconeogenesis in fat body of the adult cockroach, Periplaneta

Insect Biochemistry, 1978

Research paper thumbnail of The intracellular distribution of enzymes of carbohydrate degradation in the fat body of the adult male cockroach

Insect Biochemistry, 1978